Abstract：
:In this paper we propose a powerful procedure to measure functional activation of the mouse delta-opioid receptor transiently expressed in mammalian cells. Receptor stimulation was assessed using a population of electroporated COS cells, transfected at a 50% efficiency. Under those conditions, agonist-promoted activation of the receptor was measured by [35S]GTP gamma S binding. Both BW373U86, an alkaloid compound, and DADLE, a peptide agonist, elicited increase of specific [35S]GTP gamma S binding representing 300% of basal level. Maximal activation was compared to that obtained for the cloned receptor stably expressed in CHO cells. Agonist efficacy was similar in both expressions systems, demonstrating the high sensitivity of the proposed method applied to transient expression. Finally dose-response curves were found highly reproducible across transfection experiments, opening the possibility for a direct comparison of distinct recombinant receptor preparations. This method represents a powerful tool for the study of opioid signal transduction at the receptor level. It may also be extended to investigate signalling properties of other Gi/Go coupled receptors.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Befort K,Tabbara L,Kieffer BLdoi
10.1007/BF02532371subject
Has Abstractpub_date
1996-11-01 00:00:00pages
1301-7issue
11eissn
0364-3190issn
1573-6903journal_volume
21pub_type
杂志文章abstract：:Protein kinase A (PKA) plays a crucial role in tau hyperphosphorylation, an early event of Alzheimer disease (AD), and 17beta-estradiol replacement in aging women forestalls the onset of AD. However, the role of estradiol in PKA-induced tau hyperphosphorylation is not known. Here, we investigated the effect of 17beta-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9638-4
更新日期：2008-09-01 00:00:00
abstract：:Astrocyte-neuron communication has recently been proposed as a potential mechanism participating to synaptic transmission. With the development of this concept and accumulating evidences in favor of a modulation of synaptic transmission by astrocytes, has emerged the term gliotransmission. It refers to the capacity of...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-012-0807-0
更新日期：2012-11-01 00:00:00
abstract：:We have developed a coupled Xenopus oocyte expression system for evaluating the functional effects of mutations in known or suspected adhesion molecules, which allows for a very rapid assessment of intercellular adhesion. As a model protein, we first used Protein zero (Po), an adhesion molecule that mediates self-adhe...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1010999622760
更新日期：2001-06-01 00:00:00
abstract：:Evidence that brain glutamatergic activity is pathologically elevated in bipolar disorder suggests that mood stabilizers are therapeutic in the disease in part by downregulating glutamatergic activity. Such activity can involve the second messenger, arachidonic acid (AA, 20:4n - 6). We tested this hypothesis with rega...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9700-2
更新日期：2008-11-01 00:00:00
abstract：:Direct evidence for the excitotoxicity of 3-N-oxalyl-L-alpha,beta-diaminopropionic acid (ODAP), the Lathyrus sativus neurotoxin has been studied by examining the binding of chemically synthesized [2,3 3H]ODAP ([3H]ODAP) to synaptic membranes. [3H]ODAP binding to membranes was mostly nonspecific, with only a very low s...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020782119057
更新日期：1998-09-01 00:00:00
abstract：:We investigated the effects of H(2)O(2)-induced oxidative stress on the delayed-rectifier current (IK(DR)), neuronal physiological and morphological properties. Measurements were obtained from hippocampal CA1 neurons in control solution and from the same neurons after exposure to oxidative stress (short- and long-term...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9287-z
更新日期：2007-07-01 00:00:00
abstract：:The central control of growth hormone (GH) secretion from the pituitary gland is ultimately achieved by the interaction between two hypothalamic neurohormones, somatostatin which inhibits and growth hormone-releasing hormone (GHRH) which stimulates GH release. The regulation of the somatostatin and GHRH release from t...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-005-9017-3
更新日期：2006-02-01 00:00:00
abstract：:Serotonin (5HT) synthesis in brain is influenced by precursor (tryptophan (TRP)) concentrations, which are modified by food ingestion. Hence, in rats, a carbohydrate meal raises brain TRP and 5HT; a protein-containing meal does not, but little attention has focused on differences among dietary proteins. Recently, sing...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0382-1
更新日期：2011-03-01 00:00:00
abstract：:The potassium-stimulated release of acetylcholine, norepinephrine, serotonin, glutamate, and 4-aminobutyrate from superfused rat cortical slices was studied during hypoxia. A reduction in oxygen tensions from 603 +/- 6 to 22 +/- 2 mm Hg selectively altered the calcium-dependent efflux of these neurotransmitters, but d...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964800
更新日期：1984-08-01 00:00:00
abstract：:Recent evidence suggests that young rodents submitted to high fructose (FRU) diet develop metabolic, and cognitive dysfunctions. However, it remains unclear whether these detrimental effects of FRU intake can also be observed in middle-aged mice. Nine months-old C57BL/6 female mice were fed with water (Control) or 10%...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03135-7
更新日期：2020-12-01 00:00:00
abstract：:The effects of palmitate on intracellular and extracellular amino acid concentrations of cultured astrocytes was studied. Exposure of astrocytes to either 0.72 mM or 0.36 mM palmitate was associated with a significant reduction in the intracellular pool of glutamine and taurine. In contrast, the intracellular concentr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01000040
更新日期：1989-04-01 00:00:00
abstract：:GABAergic and cholinergic synaptosome populations were isolated by immunomagnetophoresis. Analysis of 8 amino acids showed that the GABAergic population was enriched in GABA (3 fold). The cholinergic population was enriched in citrulline (5 fold). CCK was found in both populations, but was enriched in the GABAergic. N...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00967242
更新日期：1993-04-01 00:00:00
abstract：:Specific [3H]MK801 binding to rat brain NMDA receptors after the administration of the convulsant drug 3-mercaptopropionic acid (MP) and the adenosine analogue cyclopentyladenosine (CPA) was studied by means of a quantitative autoradiographic method. MP administration (150 mg/kg, i.p.) caused significant decreases in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020708603495
更新日期：1998-10-01 00:00:00
abstract：:Repeated electroconvulsive shock is an effective treatment for affective disorders. Striatum, hippocampus and brainstem are involved in affective disorders. Sodium-potassium/ATPase is of paramount importance for the proper functioning of the brain and its involvement in the affective disorders has been claimed for a l...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9046-6
更新日期：2006-04-01 00:00:00
abstract：:Neuronal perikarya were isolated, using bulk preparative procedures, from bovine brains. Synaptosomes, neuronal perikarya, and brain homogenates had similar ganglioside patterns, with the synaptosomes containing at least four times more total ganglioside per mg protein than the neuronal perikarya and twice that of the...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966108
更新日期：1976-04-01 00:00:00
abstract：:Neurons of the rat cerebral hemispheres are known to undergo a postnatal shift to a short DNA repeat length. In the present study we report that rat neuronal nuclei are more sensitive to digestion with DNAse I when isolated at a developmental stage after the shift in neuronal DNA repeated length compared to nuclei iso...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965136
更新日期：1982-08-01 00:00:00
abstract：:Adaptive changes in serotonergic 5HT1 receptor signalling are believed to underlie the therapeutic effectiveness of antidepressant drugs. Since cells are continuously exposed to neurotransmitters/neuromodulators, spatially and temporally integrated, the responsiveness of a receptor system is dependent upon the physio-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9582-8
更新日期：2008-07-01 00:00:00
abstract：:Excessive hyperbilirubinemia can cause irreversible neurological damage in the neonatal period. However, the complete understanding of the pathogenesis of unconjugated bilirubin (UCB) encephalopathy remains a matter of debate. This study investigates whether UCB inhibits the endocytosis of cationized ferritin (CF) by ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1011608017870
更新日期：2001-07-01 00:00:00
abstract：:Zinc (Zn2+) was shown to invariably inhibit muscimol-stimulated 36Cl- uptake by synaptoneurosomes in the cerebral cortex, hippocampus and cerebellum. The Zn2+ sensitivity of the GABAA receptor-gated 36Cl- uptake in the cerebral cortex was comparable to that in the hippocampus, whereas the uptake in the cerebellum was ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02532346
更新日期：1996-08-01 00:00:00
abstract：:This article puts forward the hypothesis that the Low Density Lipid Receptor (LDLR) is one of the molecules that is involved in the clearance of amyloid proteins in the brain and that it may play a role in Alzheimer's Disease (AD) via its up-regulation by statins. The hypothesis is built on the following observations:...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-006-9084-0
更新日期：2006-06-01 00:00:00
abstract：:Chronic acrylamide (ACR) exposure induces peripheral-central axonopathy in occupational workers and laboratory animals, but the underlying mechanisms remain unclear. In this study, we first investigated the effects of ACR on slow axonal transport of neurofilaments in cultured rat dorsal root ganglia (DRG) neurons thro...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1782-z
更新日期：2016-05-01 00:00:00
abstract：:The elevated plus maze (EPM) is an animal model of anxiety used to test the effects of anxioselective drugs. The loss of the anxiolytic effect of drugs during the second exposure to the EPM is called the "one trial tolerance" (OTT) phenomenon. The present study was designed to investigate the relationship between the ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1051-y
更新日期：2013-07-01 00:00:00
abstract：:Hypoxia-induced oxidative stress is one of the major hallmark reasons underlying brain dysfunction. In the present manuscript, we have used CoCl2-induced hypoxic mice to investigate alterations in the activities of chief antioxidative stress enzymes- superoxide dismutase (SOD) and catalase (CAT) and expression of thei...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1388-x
更新日期：2014-09-01 00:00:00
abstract：:Epilepsy is one of the oldest neurological conditions known to humankind. It is known that oxidative stress and generation of reactive oxygen species are a cause and consequence of epileptic seizures. Although recent years have seen tremendous progress in the molecular biology and metabolism of selenium, we still know...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0015-8
更新日期：2009-12-01 00:00:00
abstract：:The lysosomal degradation of several sphingolipids requires the presence of four small glycoproteins called saposins, generated by proteolytic processing of a common precursor, prosaposin. Saposins share several structural properties, including six similarly located cysteines forming three disulfide bridges with the s...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022530508763
更新日期：1999-02-01 00:00:00
abstract：:We have synthesized the tertiary amines of pyridostigmine and neostigmine, 3-pyridinol dimethylcarbamate (norpyridostigmine) and 3-dimethylaminophenol dimethylcarbamate (norneostigmine) respectively, and we have tested their abilities to cross the blood-brain barrier and inhibit mouse brain AChE activity. The in vivo ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973747
更新日期：1990-06-01 00:00:00
abstract：:This review presents data on metabotropic uridine nucleotide receptor subtypes (P2UR) activated by UTP, sometimes also by UDP and/or ATP. Some chemical details of receptor subtypes and ligand interactions are described. Ligand-activated P2UR subtypes may couple to different second messengers, yet little is known about...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022487128766
更新日期：1997-08-01 00:00:00
abstract：:Olfactory ensheathing cells (OECs) and Schwann cells (SCs) are closely-related cell types with regeneration-promoting properties. Comparative gene expression analysis is particularly relevant since it may explain cell type-specific effects and guide the use of each cell type into special clinical applications. In the ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1006-3
更新日期：2013-05-01 00:00:00
abstract：:This study was designed to investigate the protective effects of extracellular superoxide dismutase (SOD3) against hydrogen peroxide (H2O2) induced damage in human neuroblastoma SH-SY5Y cells and to elucidate the mechanisms responsible for this beneficial effect. SOD3-overexpressing SH-SY5Y cells were generated by ade...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1897-x
更新日期：2016-07-01 00:00:00
abstract：:Pig brain extracts from both soluble and membrane fractions were found to contain potent inhibitors for GABA synthesizing enzyme, GAD, referred to as endogenous GAD inhibitors (EGIs) and for the binding of GABA agonist, muscimol, referred to as muscimol binding inhibitors (MBIs). EGIs and MBIs were first purified thro...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966871
更新日期：1992-01-01 00:00:00
