Selective alteration of neurotransmitter release by low oxygen in vitro.

abstract：:The present study examined the potential membrane retention of desipramine (DMI) following exposures of 293-hNET cells to DMI, and its effect on [3H]NE uptake. Incubation of cells with 500 nM DMI for 1 h or 1 day persistently inhibited the uptake of [3H]NE up to 7 days, despite daily repeated washing of cells with dru...

journal_title：Neurochemical research

authors： Zhu MY,Kyle PB,Hume AS,Ordway GA

更新日期：2004-02-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a promising biological marker and prognostic indicator in many neurological diseases. Although VEGF concentrations in plasma and cerebrospinal fluid (CSF) are increasingly reported, CSF-VEGF stability pre- and during-assay procedures is seldom evaluated. In the current stud...

journal_title：Neurochemical research

authors： Yang J,Dombrowski SM,Deshpande A,Krajcir N,El-Khoury S,Krishnan C,Luciano MG

更新日期：2011-11-01 00:00:00

abstract：:Proteasome inhibition has been observed in many neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Here, the effect of proteasome inhibition on the morphology of cultured rat cortical astrocytes was investigated. Increasing evidence suggests that the function of astrocytes is related close...

journal_title：Neurochemical research

authors： Ren QG,Yu Y,Pan DJ,Luo X,Wang XZ,Wang W

更新日期：2009-05-01 00:00:00

abstract：:Ecto-nucleotidases play a pivotal role in terminating the signalling via ATP and in producing adenosine, a neuromodulator in the nervous system. We have now investigated the pattern of adenosine formation with different concentrations of extracellular ATP in rat hippocampal nerve terminals. It was found that adenosine...

journal_title：Neurochemical research

authors： Cunha RA

更新日期：2001-09-01 00:00:00

abstract：:Disruptive effects of calcium upon neurofilaments and glial filaments were studied in white matter of rat optic nerve and spinal cord and in rat peripheral nerve. Filament ultrastructure and tissue protein composition were compared following a calcium influx into excised tissues. A calcium influx was induced by freeze...

journal_title：Neurochemical research

authors： Schlaepfer WW,Zimmerman UP

更新日期：1981-03-01 00:00:00

abstract：:Discrete populations of anti-S49 antibodies were found in the antisera of Lewis rats recovered from S49-induced experimental allergic encephalomyelitis (EAE). A potent inducer of EAE in Lewis rats, S49 is a synthetic peptide representing residues 69-84 of bovine myelin basic protein but with deletions at Gly-77 and Hi...

journal_title：Neurochemical research

authors： Day ED,Hashim GA,Potter NT,Lazarus KJ

更新日期：1985-12-01 00:00:00

abstract：:The phosphoinositide signal transduction system, and particularly, phospholipase Cbeta isozymes, are relevant in the etiopathogeny of human neuropsychiatric pathologies such as depression. Stimulation of phospholipase Cbeta activity by muscarinic receptors and G proteins was determined in crude and synaptosomal membra...

journal_title：Neurochemical research

authors： Garro MA,López de Jesús M,Ruíz de Azúa I,Callado LF,Meana JJ,Sallés J

更新日期：2004-07-01 00:00:00

abstract：:Radioactive azide derivatives of tetrodotoxin (TTX) were synthesized using 2-nitro-4-azidephenyl-[3H]beta alanine for the purpose of photolabeling of the Na channel. Three azide derivatives, N1, N2 and N3, were separated by ion exchange chromatography on Bio-Rex 70 resin and reversed phase high performance liquid chro...

journal_title：Neurochemical research

authors： Uehara S,Uyemura K

更新日期：1985-08-01 00:00:00

abstract：:Protein misfolding and deposition in the brain are implicated in the etiology of numerous neurodegenerative disorders. Here, organic solutes characteristic of microorganisms adapted to hot environments, were tested on experimental cell models of Huntington's and Parkinson's diseases. Diglycerol phosphate, di-myo-inosi...

journal_title：Neurochemical research

authors： Jorge CD,Ventura R,Maycock C,Outeiro TF,Santos H,Costa J

更新日期：2011-06-01 00:00:00

abstract：:The release of the inhibitory neurotransmitter GABA is generally enhanced under potentially cell-damaging conditions. The properties and regulation of preloaded [3H]GABA release from mouse hippocampal slices were now studied in free radical-containing medium in a superfusion system. Free radical production was induced...

journal_title：Neurochemical research

authors： Saransaari P,Oja SS

更新日期：2008-03-01 00:00:00

abstract：:The effect of postdecapitative ischemia (5 min at 37 degrees) and hypoxia (5% O2, 95% N2, 30 min) on the distribution of protein radioactivity in the cellular fractions of guinea pig cerebral cortex was studied. Ischemic conditions resulted in the increase of total radioactivity level and protein content in the mitoch...

journal_title：Neurochemical research

authors： Rossowska M,Zalewska T

更新日期：1979-02-01 00:00:00

abstract：:Ethyl pyruvate (EP) has recently been reported to afford protection against neurodegenerative disorders. However, the mechanism underlying EP-mediated neuroprotection remains to be elucidated. Because peroxynitrite has been extensively implicated in the pathogenesis of various forms of neurodegenerative disorders via ...

journal_title：Neurochemical research

authors： Chen W,Jia Z,Zhu H,Zhou K,Li Y,Misra HP

更新日期：2010-02-01 00:00:00

abstract：:Analysis of gene expression in the brain is a valuable tool to study the function of the brain under normal and pathological conditions. Although there are many techniques used to measure gene expression the validity of any such experiment is directly related to the quality of the RNA in the samples. The most readily ...

journal_title：Neurochemical research

authors： Van Deerlin VM,Gill LH,Nelson PT

更新日期：2002-10-01 00:00:00

abstract：:Schwann cell biosynthesis of glycolipids was studied by in vitro incorporation of [3H]galactose into neonatal rat sciatic nerves before and after endoneurial explant culture and in culture of purified Schwann cells. In neonatal nerves prior to culture, [3H]galactose was actively incorporated into galactocerebrosides (...

journal_title：Neurochemical research

authors： Yao JK,Windebank AJ,Poduslo JF,Yoshino JE

更新日期：1990-03-01 00:00:00

abstract：:The tyrosine phosphatase inhibitor BpV(phen) stimulated a concentration-dependent increase of phospholipase C (PLC) activity in bovine adrenal medullary chromaffin cells. This response was accompanied by an increase in PLCgamma1 tyrosine phosphorylation and its cytosketetal translocation. Insulin, at high concentratio...

journal_title：Neurochemical research

authors： Roberts-Thomson EL,Herd LM,Saunders HI,Dunkley PR,Bunn SJ

更新日期：2004-10-01 00:00:00

abstract：:We have previously reported that angiotensin receptor blockade reduces reperfusion hemorrhage in a suture occlusion model of stroke, despite increasing matrix metalloproteinase (MMP-9) activity. We hypothesized that candesartan will also decrease hemorrhage associated with delayed (6 h) tissue plasminogen activator (t...

journal_title：Neurochemical research

authors： Ishrat T,Pillai B,Ergul A,Hafez S,Fagan SC

更新日期：2013-12-01 00:00:00

abstract：:Microglia can exacerbate central nervous system disorders, including stroke and chronic progressive neurodegenerative diseases such as Alzheimer disease. Mounting evidence points to ion channels expressed by microglia as contributing to these neuropathologies. The Chloride Intracellular Channel (CLIC) family represent...

journal_title：Neurochemical research

authors： Skaper SD,Facci L,Giusti P

更新日期：2013-09-01 00:00:00

abstract：:Adult neural stem cells can be isolated from various regions of the rat brain and seem to have multilineage differentiation potential. In this study, we investigated the hypothesis that global protein expression of adult neural stem cells isolated from rat hippocampus is changed during in vitro differentiation. After ...

journal_title：Neurochemical research

authors： Maurer MH,Feldmann RE Jr,Fütterer CD,Butlin J,Kuschinsky W

更新日期：2004-06-01 00:00:00

abstract：:Harpagophytum procumbens, popularly known as devil's claw, is a plant commonly used in the treatment of diseases of inflammatory origin. The anti-inflammatory effects of H. procumbens have been studied; however, the mechanism of action is not elucidated. It is known that excess of reactive oxygen and nitrogen species ...

journal_title：Neurochemical research

authors： Schaffer LF,Peroza LR,Boligon AA,Athayde ML,Alves SH,Fachinetto R,Wagner C

更新日期：2013-11-01 00:00:00

abstract：:This work evaluated the in vitro effect of thiazolidin-4-ones on the activity of AChE (total and isoforms) isolated from the cerebral cortex, hippocampus, and lymphocytes. Kinetic parameters were evaluated and molecular docking was performed. Our results showed that thiazolidinones derived from 4-(methylthio)benzaldeh...

journal_title：Neurochemical research

authors： da Silva DS,Soares MSP,Martini F,Pesarico AP,de Mattos BDS,de Souza AA,da Silva CEH,Scaini JLR,Machado KDS,Wayne Nogueira C,Spanevello RM,Cunico W

更新日期：2020-02-01 00:00:00

abstract：:Exposure of guinea pig brain slices to low concentrations (10 microM) of NMDA caused decreases in PCr and ATP within 30 min, with a slower decrease in NAA and increase in lactate, both detectable after 1 h. Exposure to NMDA for over 1 h or at higher concentrations caused further increases in lactate and decreases in N...

journal_title：Neurochemical research

authors： Thatcher NM,Badar-Goffer RS,Ben-Yoseph O,McLean MA,Morris PG,Prior MJ,Taylor A,Bachelard HS

更新日期：2002-02-01 00:00:00

abstract：:Mice with a heterozygous knock-out of the myelin protein P0 gene (P0+/-) develop a neuropathy similar to human Charcot-Marie-Tooth disease. They are indistinguishable from wild-types (WT) at birth and develop a slowly progressing demyelinating neuropathy. The aim of this study was to investigate whether the regenerati...

journal_title：Neurochemical research

authors： Rosberg MR,Alvarez S,Krarup C,Moldovan M

更新日期：2013-06-01 00:00:00

abstract：:We have previously shown that angiotensin II (Ang II) stimulates astrocyte growth through activation of ERK1/2 mitogen activated protein (MAP) kinases. In the current study, we determined whether Ang II stimulates the expression of c-fos, c-jun and c-myc in brainstem astrocyte cultures. Reverse transcriptase-PCR analy...

journal_title：Neurochemical research

authors： Delaney J,Chiarello R,Villar D,Kandalam U,Castejon AM,Clark MA

更新日期：2008-03-01 00:00:00

abstract：:Two models of perturbed cerebellar ontogenesis were obtained by a single administration of methylazoxymethanol (MAM), a potent antimitotic agent, to mouse pups either on the day of birth (MAM0 mice) or at postnatal day 5 (MAM5 mice). The alterations of the cerebellar GABAergic system were studied by measuring glutamic...

journal_title：Neurochemical research

authors： Bacon E,Girard C,de Barry J,Gombos G

更新日期：1992-07-01 00:00:00

abstract：:We measured the activity of several acid hydrolases in oligodendrocyte and mixed glial (predominantly astrocytic) cell cultures prepared from neonatal rat cerebra. When compared with the mixed glial cultures, the cultured oligodendrocytes exhibited higher levels for all the hydrolases when activities were normalized t...

journal_title：Neurochemical research

authors： Bradel EJ,Sloan HR

更新日期：1988-10-01 00:00:00

abstract：:We recently presented evidence that distinct morphine and enkephalin receptors coexist in an opioid receptor complex (Mol. Pharmacol. 21:548-557, 1982). In this paper, we present data which demonstrate that in the presence of sodium leucine enkephalin noncompetitively inhibits the binding of [3H]naloxone to a crude pa...

journal_title：Neurochemical research

authors： Rothman RB,Westfall TC

更新日期：1983-07-01 00:00:00

abstract：:(4S)-4-Amino-5,6-heptadienoic acid [S)-gamma-allenyl-GABA; MDL 72483) is a potent inactivator of brain GABA-T in mice; (ED50 (i.p.) = 60 mg.kg-1; ED50 (oral) = 70 mg.kg-1). Its anticonvulsant effects against 3-mercaptopropionic acid (MPA)-induced seizures in mice is related to the elevation of whole brain GABA concent...

journal_title：Neurochemical research

authors： Sarhan S,Casara P,Knödgen B,Seiler N

更新日期：1991-03-01 00:00:00

abstract：:Nicotine is the principal psychoactive ingredient in cigarette smoke, and has been associated with health problems in humans. However, the pure form of nicotine may prove to be a valuable pharmaceutical agent for the treatment of AD. However, the beneficial effects of nicotine remain a matter of much controversy. In o...

journal_title：Neurochemical research

authors： Shim SB,Lee SH,Chae KR,Kim CK,Hwang DY,Kim BG,Jee SW,Lee SH,Sin JS,Bae CJ,Lee BC,Lee HH,Kim YK

更新日期：2008-09-01 00:00:00

abstract：:Experimental evidence has suggested that the high endogenous levels of taurine found in the rat heart and retina are maintained to a large extent by transport processes out of the blood, rather than by endogenous biosynthesis. When these high levels are depleted, dysfunction ensues. In vitro studies have shown that be...

journal_title：Neurochemical research

authors： Lake N,De Marte L

更新日期：1988-10-01 00:00:00

abstract：:Specific [3H]MK801 binding to rat brain NMDA receptors after the administration of the convulsant drug 3-mercaptopropionic acid (MP) and the adenosine analogue cyclopentyladenosine (CPA) was studied by means of a quantitative autoradiographic method. MP administration (150 mg/kg, i.p.) caused significant decreases in ...

journal_title：Neurochemical research

authors： Giraldez L,Girardi E

更新日期：1998-10-01 00:00:00