Abstract:
:Neuronal perikarya were isolated, using bulk preparative procedures, from bovine brains. Synaptosomes, neuronal perikarya, and brain homogenates had similar ganglioside patterns, with the synaptosomes containing at least four times more total ganglioside per mg protein than the neuronal perikarya and twice that of the homogenate. Synaptosomes had 26-33 nmol total sialic acid/mg protein, while the neurons had only 15-17 nmol. Determination of ganglioside sialidase activity showed that neuronal perikarya had very low levels (negligible), in comparison with synaptosomes or whole-brain homogenates. Trypsin treatment during the isolation procedure enhanced sialidase activity two-to threefold in the particulate fraction of the whole-brain homogenate. Determination of the distribution of sialidase activity in the fractions obtained during the isolation of the neuronal perikarya showed that the sialidase activity was associated with the myelin, broken-off dendritic processes, and glial-cell fractions that banded in the less dense sucrose.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Schengrund CL,Nelson JTdoi
10.1007/BF00966108subject
Has Abstractpub_date
1976-04-01 00:00:00pages
171-80issue
2eissn
0364-3190issn
1573-6903journal_volume
1pub_type
杂志文章abstract::miR-126 which is considered one of the most important miRNAs for maintaining vascular integrity, plays an important role in neuroprotection after cerebral ischemia-reperfusion (I-R). Moreover, vascular endothelial growth factor A (VEGFA), sprouty-related EVH1 domain-containing protein 1 (SPRED1), and Raf-1 are also in...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-02986-4
更新日期:2020-05-01 00:00:00
abstract::The composition of retrogradely transported axonal proteins was examined by acrylamide gel electrophoresis and gel autoradiography in the experimental neuropathy induced in rats by p-bromophenylacetylurea (BPAU). Protein composition was normal during the early phase of retrograde transport but showed significant abnor...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966781
更新日期:1993-06-01 00:00:00
abstract::Despite the consensus that activation of TWIK-related spinal cord K+ (TRESK) might contribute to the pathogenesis of chronic pain, the specific mechanisms underlying the transfer and development of pain signals still remain obscure. In the present study, we validated that TRESK was expressed in neurons instead of glia...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2170-z
更新日期:2017-05-01 00:00:00
abstract::Spinal cord injury (SCI), a severe health problem in worldwide, was commonly associated with functional disability and reduced quality of life. As the expression of brain-derived neurotrophic factor (BDNF) was substantial event in injured spinal cord, we hypothesized whether BDNF-overexpression could be in favor of th...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1970-5
更新日期:2016-10-01 00:00:00
abstract::Chicken neurons in culture display measurable activities of mono- and diacylglycerol lipases. Treatment of chicken neuronal cultures with gangliosides (10(-8)M to 10(-5)M) resulted in a time and dose dependent increase in monoacylglycerol lipase activity. The diacylglycerol lipase showed significant increase in specif...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966702
更新日期:1991-11-01 00:00:00
abstract::Decreased antioxidant activity is considered as one of the causes of tardive dyskinesia in schizophrenic patients in a prolonged neuroleptic treatment course. Haloperidol (HAL) has been hypothesized to increase oxidative stress, while clozapine (CLO) would produce less oxidative damage. The objective was to determine ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9311-3
更新日期:2007-08-01 00:00:00
abstract::It is reported that CNS hemorrhage causes membrane dysfunction and may exacerbate this damage as a result of secondary ischemia or hypoxia. Since hyperbaric oxygenation improves oxygen metabolism, it may reduce this membrane damage. The present study was conducted to reveal whether hyperbaric oxygenation influences me...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966765
更新日期:1993-09-01 00:00:00
abstract::Aneurysmal subarachnoid hemorrhage still has a high mortality and morbidity despite notable advances in surgical approaches to cerebral aneurysm (CA). We examined the role of aspirin in vascular inflammation and degeneration. CA was induced in male Sprague-Dawley rats by ligating left common carotid artery and bilater...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1603-4
更新日期:2015-07-01 00:00:00
abstract::We investigated the effects of H(2)O(2)-induced oxidative stress on the delayed-rectifier current (IK(DR)), neuronal physiological and morphological properties. Measurements were obtained from hippocampal CA1 neurons in control solution and from the same neurons after exposure to oxidative stress (short- and long-term...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9287-z
更新日期:2007-07-01 00:00:00
abstract::Interleukin (IL)-18, a member of the IL-1 cytokine family, is an important mediator of peripheral inflammation and host defence responses. However, although IL-1 is a key proinflammatory cytokine in the brain, little is known about IL-18 changes in glial cells under excitotoxic neurodegeneration. In this study, we cha...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9434-6
更新日期:2008-01-01 00:00:00
abstract::Association of the candidate gene DLST with late-onset Alzheimer's disease (LOAD) risk has been suggested on the basis of case-control studies. This gene, located on chromosome 14q24.3, encodes a subunit of a mitochondrial component known to be defective in AD, the alpha-ketoglutarate dehydrogenase complex. Positive r...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000014833.54481.1d
更新日期:2004-03-01 00:00:00
abstract::Emerging data suggests that mitochondrial dysfunction is prominently involved in Alzheimer disease (AD) progression. Sirtuin-3 (Sirt3) is a member of the sirtuin family of nicotinamide adenine dinucleotide dependent deacetylases that regulates a variety of mitochondrial functions and suppresses mitochondria-related ph...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1630-1
更新日期:2015-08-01 00:00:00
abstract::Effects of methylmercury (MetHg) on the specific [3H]flunitrazepam binding were studied in rat cortical and cerebellar P2-fractions in vitro. MetHg did not affect significantly the specific [3H]flunitrazepam binding in unwashed P2-fraction but increased it marginally (by 16%) at 100 microM in washed P2-fraction, in bo...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01705532
更新日期:1995-06-01 00:00:00
abstract::Focal epileptic seizures can in some patients be managed by inhibiting γ-aminobutyric acid (GABA) uptake via the GABA transporter 1 (GAT1) using tiagabine (Gabitril®). Synergistic anti-seizure effects achieved by inhibition of both GAT1 and the betaine/GABA transporter (BGT1) by tiagabine and EF1502, compared to tiaga...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03017-y
更新日期:2020-07-01 00:00:00
abstract::Selective and limited proteolysis is a key step in the post-translational modification of peptide hormone precursors. This process appears to involve a proteolytic machinery including highly specific endoproteases. Some of the enzyme systems possibly involved in the processing of pro-neuropeptides will be described an...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00966318
更新日期:1987-10-01 00:00:00
abstract::Poly(A)-RNA prepared from the brains of 30-day-old male rats has been shown to direct the synthesis of neuron-specific enolase (NSE) in a cell-free system derived from rabbit reticulocytes. The newly synthesized polypeptides were immunoprecipitated and analyzed on SDS-polyacrylamide slab gels. Autoradiography indicate...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964225
更新日期:1980-04-01 00:00:00
abstract::The peroxisome proliferator-activated receptor gamma (PPARγ) is a ligand-activated transcriptional factor that belongs to the nuclear hormone receptor superfamily. PPARγ was initially identified through its role in the regulation of glucose and lipid metabolism and cell differentiation. It also influences the expressi...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-014-1377-0
更新日期:2015-02-01 00:00:00
abstract::In this study, changes of the expression of two mitochondrial and two nuclear genes encoding the subunits of cytochrome c oxidase (CO) and NADH dehydrogenase (ND) were studied in the hippocampus, inferior parietal lobule, and cerebellum of 10 Alzheimer's disease (AD) and 10 age-matched control subjects. The altered pr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020783614031
更新日期:1999-06-01 00:00:00
abstract::Convection enhanced delivery of 6-hydroxydopamine (6-OHDA) to the rat striatum results in a model of Parkinson's disease. An important feature of this unilateral model is the progressive loss of dopaminergic (DA) neurons over the course of several weeks. To improve the understanding of this model, gene expression chan...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0133-3
更新日期:2010-05-01 00:00:00
abstract::We have previously shown that 5-methyltetrahydrofolate influences neuro-secretion. The present study more precisely characterises the processes involved and considers one probable site of action. Focusing on the tyrosine-noradrenalin axis in cerebellum we showed 5-methyltetrahydrofolate causes a significant reduction ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01705542
更新日期:1995-06-01 00:00:00
abstract::A variety of naturally occurring amino acids, their isomers, and synthetic analogs were tested for their ability to inhibit uptake of [3H]glutamate into presynaptic vesicles from bovine cerebral cortex. Strongest inhibition (Ki < 1mM) was observed for trans-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) and erythr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966925
更新日期:1993-01-01 00:00:00
abstract::Zonisamide (ZNS), an antiepileptic drug having beneficial effects also against Parkinson's disease symptoms, has proven to display an antioxidant effects in different experimental models. In the present study, the effects of ZNS on rotenone-induced cell injury were investigated in human neuroblastoma SH-SY5Y cells dif...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1181-2
更新日期:2013-12-01 00:00:00
abstract::The Editors have retracted this article [1] following an investigation conducted by the journal. After publication concerns were raised regarding interpretation of the data presented in Fig. 4. The Editors requested additional data and clarification to confirm interpretation of data results. After further review, the ...
journal_title:Neurochemical research
pub_type: 杂志文章,撤回出版物
doi:10.1007/s11064-020-03077-0
更新日期:2020-09-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is a promising biological marker and prognostic indicator in many neurological diseases. Although VEGF concentrations in plasma and cerebrospinal fluid (CSF) are increasingly reported, CSF-VEGF stability pre- and during-assay procedures is seldom evaluated. In the current stud...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0517-z
更新日期:2011-11-01 00:00:00
abstract::The aim of the present study was to investigate possible membrane and genomic effects of corticosterone on the noradrenergic system of the rat brain. Corticosterone effects were studied in vivo by treating rats s.c. with 10 mg/kg corticosterone for 7 or 14 days. In the first two experiments corticosterone significantl...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966670
更新日期:1992-03-01 00:00:00
abstract::A synthetic peptide corresponding to the first 28 amino acids of the Alzheimer disease amyloid beta/A4 peptide (3.2 kDa) aggregated to a high molecular weight (15 kDa) on SDS/urea polyacrylamide gels. Proteinase K, V8 protease, trypsin, and endopeptidase Lys-C readily degraded the aggregate. By contrast, when digested...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00974579
更新日期:1992-04-01 00:00:00
abstract::The exposure to hypobaric hypoxia increased lipid peroxidation as indicated by thiobarbituric acid-reactive substances [TBARS] in rat brain. Plasma lactate/pyruvate ratio was used as a marker of hypoxia. We compared the protective effect of alpha-tocopherol with the effect of L-carnitine or phosphocreatine. Rats pretr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020339530924
更新日期:2002-09-01 00:00:00
abstract::The long-acting opiate antagonist naltrexone hydrochloride was administered by intraperitoneal injection, in a dose response protocol, to adult rats. The drug was used to observe effects of opiate receptor blockade on cells of the pituitary gland and adjacent hypothalamus. At higher drug doses (5 mg/kg or 10 mg/kg), n...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964168
更新日期:1984-02-01 00:00:00
abstract::Rat obturator nerve 16S acetylcholinesterase (16S AChE) was separated by sucrose gradient velocity sedimentation and compared to the 16S form of AChE similarly derived from endplate regions of anterior gracilis muscles. The 16S AChE from both tissues could only be extracted in high ionic strength buffer; as it aggrega...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965031
更新日期:1981-09-01 00:00:00
abstract::The particulars regarding the amine precursor therapy for treatment of neuropsychiatric disorders and their neurochemical background are described historically. Furthermore, peculiar or artificial amino acids which are metabolized into the brain amine or their derivatives and promised their clinical application are al...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF02529142
更新日期:1996-02-01 00:00:00