Dynamic Changes in miR-126 Expression in the Hippocampus and Penumbra Following Experimental Transient Global and Focal Cerebral Ischemia-Reperfusion.


:miR-126 which is considered one of the most important miRNAs for maintaining vascular integrity, plays an important role in neuroprotection after cerebral ischemia-reperfusion (I-R). Moreover, vascular endothelial growth factor A (VEGFA), sprouty-related EVH1 domain-containing protein 1 (SPRED1), and Raf-1 are also involved in physiological processes of vascular endothelial cells (ECs). This study investigated how miR-126 changes with reperfusion time in different brain tissues after global cerebral ischemia and focal cerebral ischemia and examined the underlying mechanism miR-126 involving VEGFA, SPRED1, and Raf-1 after I-R. The results indicated decreases in the levels of miR-126-3p and miR-126-5p expression in mice and gerbils after I-R, consistent with the results after oxygen and glucose deprivation and reperfusion (OGD/R) in PC12 cells. Glial cells were activated as neuronal damage gradually increased after I-R. Inhibition of miR-126-3p exacerbated the OGD/R-induced cell death and reduced cell viability. After miR-126-3p inhibition, the levels of SPRED1 and VEGFA expression were increased, and p-Raf-1 expression was decreased after OGD/R. Moreover, based on the intervention of miR-126-3p inhibition, we found that the expression of p-Raf-1 was significantly increased after the intervention of siSPRED1, while it was not statistically significant after intervention of siVEGFA. The reduction of miR-126 expression after global and focal cerebral ischemia exacerbated neuronal death, which was closely related to increasing the SPRED1 activation and inhibiting the Raf-1 expression.


Neurochem Res


Neurochemical research


Xiao ZH,Wang L,Gan P,He J,Yan BC,Ding LD




Has Abstract


2020-05-01 00:00:00














  • [3H] clonidine binding to rat hippocampal membranes.

    abstract::Alpha adrenergic receptor subtypes in rat hippocampal membranes were studied, using [3H] clonidine as the radioactive ligand. On the basis of competitive binding studies, using the selective antagonist-prazosin, WB-4101, and yohimbine, [3H] clonidine appeared to bind to a population of presynaptic sites that are pharm...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: George CR

    更新日期:1982-02-01 00:00:00

  • Intracellular ion channel CLIC1: involvement in microglia-mediated β-amyloid peptide(1-42) neurotoxicity.

    abstract::Microglia can exacerbate central nervous system disorders, including stroke and chronic progressive neurodegenerative diseases such as Alzheimer disease. Mounting evidence points to ion channels expressed by microglia as contributing to these neuropathologies. The Chloride Intracellular Channel (CLIC) family represent...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Skaper SD,Facci L,Giusti P

    更新日期:2013-09-01 00:00:00

  • Endoplasmic reticulum calcium release engages Bax translocation in cortical astrocytes.

    abstract::Apoptosis is a highly complex form of cell death that can be triggered by alterations in Ca(2+) homeostasis. Members of the Bcl-2 family may regulate apoptosis and modulate Ca(2+) distribution within intracellular compartments. Bax, a proapoptotic member of the family, is constitutively expressed and soluble in the cy...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Morales AP,Carvalho AC,Monteforte PT,Hirata H,Han SW,Hsu YT,Smaili SS

    更新日期:2011-05-01 00:00:00

  • Inhibitors of crayfish glutamic acid decarboxylase.

    abstract::Crayfish glutamic acid decarboxylase (GAD), like the homologous enzymes from other species, is inhibited by carbonyl-trapping agents (e.g. aminooxyacetic acid; AOAA) and sulfhydryl reagents (e.g. 5,5'-dithiobis-(2-nitrobenzoic acid); DTNB). It also is inhibited by the product GABA, many anions (e.g. SCN- and Cl-), and...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Grossfeld RM,Yancey SW,Baxter CF

    更新日期:1984-07-01 00:00:00

  • Nicotinic receptor-elicited sodium flux in rat pheochromocytoma PC12 cells: effects of agonists, antagonists, and noncompetitive blockers.

    abstract::Nicotinic agonists stimulate 22Na flux in rat pheochromocytoma PC12 cells. The stimulatory effect of carbamylcholine is maximal at 1 mM, while the stimulatory effect of nicotine and anatoxin maximize at the same level at 100 microM and 10 microM, respectively. The tertiary amines arecolone and isoarecolone have no eff...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Daly JW,Nishizawa Y,Edwards MW,Waters JA,Aronstam RS

    更新日期:1991-04-01 00:00:00

  • Comparison and effects of acute lamotrigine treatment on extracellular excitatory amino acids in the hippocampus of PTZ-kindled epileptic and PTZ-induced status epilepticus rats.

    abstract::In this communication, the effect of acute treatment with lamotrigine (LTG) was investigated on release of main excitatory amino acids (EAA) such as glutamate (Glu) and aspartate (Asp) in the hippocampus of pentylenetetrazol (PTZ)-induced and PTZ-kindled freely moving rats using micro dialysis. The results show that, ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Deng Y,Wang M,Wang W,Ma C,He N

    更新日期:2013-03-01 00:00:00

  • Postnatal development and GABA allosteric modulation of benzodiazepine receptor binding in the vitamin B-6 deficient rat brain.

    abstract::We have measured the postnatal development and GABA modulation of benzodiazepine receptors in neuronal membranes from vitamin B-6 deficient and normal rats. In rats fed vitamin B-6 adequate and deficient diets there were age-dependent changes in [3H]flunitrazepam binding site affinity and in the number of binding site...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Pilachowski J,Guilarte TR

    更新日期:1993-12-01 00:00:00

  • Comparison of neurosphere-like cell clusters derived from dental follicle precursor cells and retinal Müller cells.

    abstract::Unrelated cells such as dental follicle precursor cells (DFPCs) and retinal Müller cells (MCs) make spheres after cultivation in serum-replacement medium (SRM). Until today, the relation and molecular processes of sphere formation from different cell types remain undescribed. Thus in this study we compared proteomes o...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Beck HC,Petersen J,Felthaus O,Schmalz G,Morsczeck C

    更新日期:2011-11-01 00:00:00

  • Age-related changes in acetylcholinesterase and its molecular forms in various brain areas of rats.

    abstract::A previous study conducted in this laboratory revealed a decrease in total cholinesterase (total ChE) in the cerebral cortex, hippocampus and striatum in aged rats (24 months) of various strains, as compared with young animals (3 months). The purpose of the present experiments was to extend the study to other brain ar...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Meneguz A,Bisso GM,Michalek H

    更新日期:1992-08-01 00:00:00

  • Granulocyte colony stimulating factor reduces brain injury in a cardiopulmonary bypass-circulatory arrest model of ischemia in a newborn piglet.

    abstract::Ischemic brain injury continues to be of major concern in patients undergoing cardiopulmonary bypass (CPB) surgery for congenital heart disease. Striatum and hippocampus are particularly vulnerable to injury during these processes. Our hypothesis is that the neuronal injury resulting from CPB and the associated circul...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Pastuszko P,Schears GJ,Greeley WJ,Kubin J,Wilson DF,Pastuszko A

    更新日期:2014-11-01 00:00:00

  • High-affinity uptake of taurine and beta-alanine in primary cultures of rat astrocytes.

    abstract::The kinetics and specificity of taurine and beta-alanine uptake were studied in primary cultures of rat astrocytes under identical experimental conditions. The uptake consisted of nonsaturable penetration and saturable high-affinity transport that was strictly sodium dependent. The cells accumulated taurine more effec...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Holopainen I,Kontro P

    更新日期:1986-02-01 00:00:00

  • Ginsennoside rd attenuates cognitive dysfunction in a rat model of Alzheimer's disease.

    abstract::Alzheimer's disease is a neurodegenerative disease characterized by the production of β-amyloid proteins and hyperphosphorylation of tau protein. Inflammation and apoptotic severity were highly correlated with earlier age at onset of Alzheimer's disease and were also associated with cognitive decline. This study aims ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Liu J,Yan X,Li L,Zhu Y,Qin K,Zhou L,Sun D,Zhang X,Ye R,Zhao G

    更新日期:2012-12-01 00:00:00

  • Acetylcholinesterase inhibitor ENA-713 protects against ischemia-induced decrease in pre- and postsynaptic cholinergic indices in the gerbil brain following transient ischemia.

    abstract::The effects of pre-treatment with ENA-713, an acetylcholinesterase (AChE) inhibitor, on changes in pre- and postsynaptic cholinergic indices in gerbil brain following transient ischemia were studied at 4 and 14 days after recirculation. In the ischemic group, hippocampal acetylcholine (ACh) level was significantly red...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Tanaka K,Ogawa N,Mizukawa K,Asanuma M,Kondo Y,Nishibayashi S,Mori A

    更新日期:1994-02-01 00:00:00

  • The antiretroviral protease inhibitor ritonavir accelerates glutathione export from cultured primary astrocytes.

    abstract::Antiretroviral protease inhibitors are a class of important drugs that are used for the treatment of human immunodeficiency virus infections. Among those compounds, ritonavir is applied frequently in combination with other antiretroviral protease inhibitors, as it has been reported to boost their therapeutic efficienc...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Arend C,Brandmann M,Dringen R

    更新日期:2013-04-01 00:00:00

  • Expression Changes of NMDA and AMPA Receptor Subunits in the Hippocampus in rats with Diabetes Induced by Streptozotocin Coupled with Memory Impairment.

    abstract::Cognitive impairment in diabetes (CID) is a severe chronic complication of diabetes mellitus (DM). It has been hypothesized that diabetes can lead to cognitive dysfunction due to expression changes of excitatory neurotransmission mediated by N-methyl-D-aspartate receptors (NMDAR) and α-amino-3-hydroxy-5-methyl-4-isoxa...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Wang XP,Ye P,Lv J,Zhou L,Qian ZY,Huang YJ,Mu ZH,Wang X,Liu XJ,Wan Q,Yang ZH,Wang F,Zou YY

    更新日期:2019-04-01 00:00:00

  • Modification of [3H]MK801 binding to rat brain NMDA receptors after the administration of a convulsant drug and an adenosine analogue: a quantitative autoradiographic study.

    abstract::Specific [3H]MK801 binding to rat brain NMDA receptors after the administration of the convulsant drug 3-mercaptopropionic acid (MP) and the adenosine analogue cyclopentyladenosine (CPA) was studied by means of a quantitative autoradiographic method. MP administration (150 mg/kg, i.p.) caused significant decreases in ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Giraldez L,Girardi E

    更新日期:1998-10-01 00:00:00

  • Brain tumor stem cells.

    abstract::Primary malignant brain cancer, one of the most deadly diseases, has a high rate of recurrence after treatment. Studies in the past several years have led to the hypothesis that the root of the recurrence may be brain tumor stem cells (BTSCs), stem-like subpopulation of cells that are responsible for propagating the t...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: Xie Z

    更新日期:2009-12-01 00:00:00

  • The effects of polyphenols on survival and locomotor activity in Drosophila melanogaster exposed to iron and paraquat.

    abstract::Parkinson's disease (PD) is a common progressive neurodegenerative disorder, for which at present no causal treatment is available. On the understanding that the causes of PD are mainly oxidative stress and mitochondrial dysfunction, antioxidants and other drugs are expected to be used. In the present study, we demons...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Jimenez-Del-Rio M,Guzman-Martinez C,Velez-Pardo C

    更新日期:2010-02-01 00:00:00

  • Molecular profiling of a 6-hydroxydopamine model of Parkinson's disease.

    abstract::Convection enhanced delivery of 6-hydroxydopamine (6-OHDA) to the rat striatum results in a model of Parkinson's disease. An important feature of this unilateral model is the progressive loss of dopaminergic (DA) neurons over the course of several weeks. To improve the understanding of this model, gene expression chan...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Na SJ,DiLella AG,Lis EV,Jones K,Levine DM,Stone DJ,Hess JF

    更新日期:2010-05-01 00:00:00

  • Differential involvement of intracellular Ca2+ in 1-methyl-4-phenylpyridinium- or 6-hydroxydopamine-induced cell viability loss in PC12 cells.

    abstract::1-Methyl-4-phenylpyridinium (MPP(+)) or 6-hydroxydopamine (6-OHDA) caused a nuclear damage, the mitochondrial membrane permeability changes, leading to the cytochrome c release and caspase-3 activation, the formation of reactive oxygen species and the depletion of GSH in PC12 cells. Nicardipine (a calcium channel bloc...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Lee DH,Han YS,Han ES,Bang H,Lee CS

    更新日期:2006-07-01 00:00:00

  • Aspirin Inhibits Degenerative Changes of Aneurysmal Wall in a Rat Model.

    abstract::Aneurysmal subarachnoid hemorrhage still has a high mortality and morbidity despite notable advances in surgical approaches to cerebral aneurysm (CA). We examined the role of aspirin in vascular inflammation and degeneration. CA was induced in male Sprague-Dawley rats by ligating left common carotid artery and bilater...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Li S,Wang D,Tian Y,Wei H,Zhou Z,Liu L,Wang D,Dong JF,Jiang R,Zhang J

    更新日期:2015-07-01 00:00:00

  • The receptor concept in 3D: from hypothesis and metaphor to GPCR-ligand structures.

    abstract::The first mentioning of the word "receptor" for the structure with which a bioactive compound should react for obtaining its specific influence on a physiological system goes back to the years around 1900. The receptor concept was adapted from the lock and key theory for the enzyme substrate and blockers interactions....

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: Kooistra AJ,de Graaf C,Timmerman H

    更新日期:2014-10-01 00:00:00

  • L-kynurenine: its synthesis and possible regulatory function in brain.

    abstract::One pathway by which tryptophan is metabolized in the brain as well as in the periphery is through cleavage of the indole ring to formylkynurenine and then kynurenine. Indoleamine-2,3-dioxygenase, the enzyme that catalyzes this cleavage, and kynurenine are distributed all across the different anatomic regions of brain...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: Gál EM,Sherman AD

    更新日期:1980-03-01 00:00:00

  • S100beta protein expression: gender- and age-related daily changes.

    abstract::S100beta is a soluble protein released by glial cells mainly under the activation of the 5-HT1A receptor. It has been reported as a neuro-trophic and -tropic factor that promotes neurite maturation and outgrowth during development. This protein also plays a role in axonal stability and the plasticity underlying long-t...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Nogueira MI,Abbas SY,Campos LG,Allemandi W,Lawson P,Takada SH,Azmitia EC

    更新日期:2009-08-01 00:00:00

  • Incorporation of [3H]ethanolamine into acetylcholine by a human cholinergic neuroblastoma clone.

    abstract::Human neuroblastoma cholinergic LA-N-2 cells were used as an experimental model to test the possibility that the methylation of phosphoethanolamine (PEtn) to phosphocholine (PCho) and free choline (Cho) (Andriamampandry et al. 1989) could contribute to acetylcholine (AcCho) synthesis. LA-N-2 cells were incubated with ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Haidar NE,Carrara M,Andriamampandry C,Kanfer JN,Freysz L,Dreyfus H,Massarelli R

    更新日期:1994-01-01 00:00:00

  • The oxidative and morphological effects of high concentration chronic toluene exposure on rat sciatic nerves.

    abstract::This study was designed to investigate the effects of chronic toluene inhalation in high concentration on lipid peroxidation, antioxidant enzyme activities and ultrastructural changes in the sciatic nerves of rats. Male Wistar albino rats (150-250 g) were divided in two experimental groups: the control and the toluene...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Coskun O,Oter S,Korkmaz A,Armutcu F,Kanter M

    更新日期:2005-01-01 00:00:00

  • 2-Deoxy-D-Glucose Exhibits Anti-seizure Effects by Mediating the Netrin-G1-KATP Signaling Pathway in Epilepsy.

    abstract::Epilepsy is a disorder of the brain characterized by an enduring predisposition to generate epileptic seizures. The glycolytic inhibitor 2-deoxy-D-glucose (2-DG) has been reported to exert antiepileptic effects by upregulating KATP subunits (kir6.1 and kir6.2). We evaluated whether 2-DG exhibits anti-seizure effect by...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Long Y,Zhuang K,Ji Z,Han Y,Fei Y,Zheng W,Song Z,Yang H

    更新日期:2019-04-01 00:00:00

  • Inhibition of vesicular glutamate uptake by Rose Bengal-related compounds: structure-activity relationship.

    abstract::Synaptic vesicular accumulation of glutamate is a vital initial step in glutamate transmission. We have previously shown that Rose Bengal, a polyhalogenated fluorescein analog, is a potent inhibitor of glutamate uptake into synaptic vesicles. Here, we report the structural features of Rose Bengal required for this inh...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Bole DG,Ueda T

    更新日期:2005-03-01 00:00:00

  • Decreased GABA receptor binding in the cerebral cortex of insulin induced hypoglycemic and streptozotocin induced diabetic rats.

    abstract::Hypoglycemia is the major problem to blood glucose homeostasis in treatment of diabetes and is associated with severe irreversible consequences including seizures, coma and death. GABAergic inhibitory function in the cerebral cortex plays an important role in controlling the excitability and responsiveness of cortical...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Antony S,Kumar TP,Kuruvilla KP,George N,Paulose CS

    更新日期:2010-10-01 00:00:00

  • The protection of novel 2-arylethenylquinoline derivatives against impairment of associative learning memory induced by neural Aβ in C. elegans Alzheimer's disease model.

    abstract::Cerebral deposition of amyloid β-peptide (Aβ), a fundamental feature of Alzheimer's disease (AD), damages the neurocytes and impairs the cognition functions and associative learning memory of AD patients. A series of novel 2-arylethenylquinoline derivatives were synthesized and evaluated in our previous study, which i...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: He Q,Huang G,Chen Y,Wang X,Huang Z,Chen Z

    更新日期:2017-11-01 00:00:00