Abstract：
:Rat obturator nerve 16S acetylcholinesterase (16S AChE) was separated by sucrose gradient velocity sedimentation and compared to the 16S form of AChE similarly derived from endplate regions of anterior gracilis muscles. The 16S AChE from both tissues could only be extracted in high ionic strength buffer; as it aggregated under low ionic strength conditions. Treatment of nerve and muscle 16S AChE with purified collagenase, in the presence of calcium, caused an identical "shift" in the enzyme's sedimentation coefficient to 17.5S. Other properties which were also equivalent for 16S AChE from both tissue sources included: an excess substrate inhibition above 2 x 10(-3) M acetylcholine and Km of 1.6 x 10(-4) M, relative sensitivity to the specific inhibitors BW284C51 (I50 of 5 x 10(-8) M) and Iso-OMPA (I50 of 5 x 10(-4) M), and a half maximal thermal inactivation at 62.5 degrees C. These and additional results indicate that the 16S forms of AChE in both tissues are analogous molecules, which have a highly asymmetric conformation probably containing a collagen-like domain. The present findings are also consistent with the view that motor neurons provide at least a fraction of the 16S AChE present at the neuromuscular junction.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Fernandez HLdoi
10.1007/BF00965031subject
Has Abstractpub_date
1981-09-01 00:00:00pages
1005-17issue
9eissn
0364-3190issn
1573-6903journal_volume
6pub_type
杂志文章abstract：:Anesthetics such as propofol can provide neuroprotective effects against cerebral ischemia. However, the underlying mechanism of this beneficial effect is not clear. Therefore, we subjected male Sprague-Dawley rats to 2 h of middle cerebral artery occlusion and investigated how post-ischemic administration of propofol...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0945-4
更新日期：2013-03-01 00:00:00
abstract：:The release of preloaded D-[3H]aspartate, an unmetabolizable analogue of L-glutamate, was studied in superfused hippocampal slices from 7-day-old and 3-month-old (adult) mice under various cell-damaging conditions, including hypoxia, hypoglycemia, ischemia, oxidative stress and the presence of free radicals and metabo...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021052725921
更新日期：1999-08-01 00:00:00
abstract：:The current study investigated the neuroprotective activity of idebenone against pilocarpine-induced seizures and hippocampal injury in rats. Idebenone is a ubiquinone analog with antioxidant, and ATP replenishment effects. It is well tolerated and has low toxicity. Previous studies reported the protective effects of ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1236-z
更新日期：2014-02-01 00:00:00
abstract：:The effect of isoproterenol, norepinephrine, dopamine, and serotonin on the accumulation of adenosine 3',5'-monophosphate (cAMP) was studied in homogeneous neuronal cultures from 8-day chick embryo hemispheres. Among the catecholamines, isoproterenol had a more pronounced effect on the accumulation of cAMP. Norepineph...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966167
更新日期：1980-10-01 00:00:00
abstract：:N-Methyl-D-aspartate (NMDA) and L-glutamate activate membrane receptors that produce substantial permeation of Na+, K+ and Ca2+ through the neuronal membrane. These ionic fluxes are intimately linked to processes that regulate neuronal survival, growth and differentiation. Intracellular free Ca2+ concentrations are th...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00966862
更新日期：1992-01-01 00:00:00
abstract：:Transgenic therapy for central neuralgia faces the problems of low expression and weak targeting and affects superficial but not deep neurons. In this study, we generated a lentivirus vector with human preproenkephalin gene (hPPE) expression driven by the transcriptional amplification strategy system (TAS) and establi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03068-1
更新日期：2020-09-01 00:00:00
abstract：:Systemic inflammation triggered by lipopolysaccharide (LPS) administration disrupts blood-brain barrier (BBB) homeostasis in animal models. This event leads to increased susceptibility of several encephalic structures to potential neurotoxicants present in the bloodstream. In this study, we investigated the effects of...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2477-z
更新日期：2018-03-01 00:00:00
abstract：:The influence of theophylline (2.5--100 mg/kg p.o.) on cyclic 3',5'-adenosine monophosphate (cAMP) and cyclic 3',5'-guanosine monophosphate (cGMP) in brain of Sprague-Dawley rats (0.5--3.0 hr after administration of theophylline) was investigated. It was found that theophylline increases cAMP and cGMP levels when admi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964436
更新日期：1979-10-01 00:00:00
abstract：:Alpha adrenergic receptor subtypes in rat hippocampal membranes were studied, using [3H] clonidine as the radioactive ligand. On the basis of competitive binding studies, using the selective antagonist-prazosin, WB-4101, and yohimbine, [3H] clonidine appeared to bind to a population of presynaptic sites that are pharm...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965058
更新日期：1982-02-01 00:00:00
abstract：:Exercise has been regarded as an effective rehabilitation strategy to facilitate motor and cognitive functional recovery after stroke, even though the complex effects associated with exercise-induced repair of cerebral ischemic injury are not fully elucidated. The enhancement of angiogenesis and neurogenesis, and the ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02728-1
更新日期：2019-04-01 00:00:00
abstract：:Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system (CNS) and exerts its actions via a number of ionotropic glutamate receptors/channels and metabotropic glutamate (mGlu) receptors. In addition to being expressed in neurons, glutamate receptors are expressed in different types of...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-008-9694-9
更新日期：2008-12-01 00:00:00
abstract：:Methylguanidine (MG) is known as not only a nephrotoxin but also as a neurotoxine. We have previously showed that MG itself generates hydroxyl radicals (*OH) in an in vitro study. In this study, we examined the inhibitory effects of ascorbate, EPC-K(1) (alpha-tocopheryl-L-ascorbate-2-O-phosphate diester), Trolox (wate...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9811-9
更新日期：2009-04-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963986
更新日期：1984-03-01 00:00:00
abstract：:Mice with a heterozygous knock-out of the myelin protein P0 gene (P0+/-) develop a neuropathy similar to human Charcot-Marie-Tooth disease. They are indistinguishable from wild-types (WT) at birth and develop a slowly progressing demyelinating neuropathy. The aim of this study was to investigate whether the regenerati...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1030-3
更新日期：2013-06-01 00:00:00
abstract：:We have previously reported that angiotensin receptor blockade reduces reperfusion hemorrhage in a suture occlusion model of stroke, despite increasing matrix metalloproteinase (MMP-9) activity. We hypothesized that candesartan will also decrease hemorrhage associated with delayed (6 h) tissue plasminogen activator (t...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1185-y
更新日期：2013-12-01 00:00:00
abstract：:Malformations of cortical development (MCD) are critical brain development disorders associated with varied abnormalities in both anatomic structures and neural functioning. It is also a very common etiology to the epilepsy, in which the alteration on excitability of cortical neurons is hypothesized as one of importan...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1936-7
更新日期：2016-09-01 00:00:00
abstract：:The microdialysis technique was used to study the effect of nitric oxide synthase (NOS) activity on taurine release. Taurine release was characterized in rat striatum that was excitotoxically lesioned compared to normal conditions. The basal taurine level of the dialysate decreased during quinolinate (QUIN) lesion in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022418231496
更新日期：1998-04-01 00:00:00
abstract：:There is growing evidence that ketone bodies (KB)-derived from fatty acid oxidation and produced during fasting or consumption of high-fat diets-can exert broad neuroprotective effects. With respect to epilepsy, KB (such as β-hydroxybutyrate or BHB, acetoacetate and acetone) have been shown to block acutely induced an...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2253-5
更新日期：2017-07-01 00:00:00
abstract：:Subacute exposure to manganese (Mn) produced Parkinson's disease-like syndrome called Manganism. Chronic onset and progression are characteristics of Manganism, therefore, this study aimed to examine Mn toxicity following chronic exposures. Male Sprague-Dawley rats were injected Mn2+ 1 and 5 mg/kg, every 10 days for 1...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03059-2
更新日期：2020-08-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-008-9885-4
更新日期：2009-06-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1262-x
更新日期：2014-04-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964927
更新日期：1983-09-01 00:00:00
abstract：:Serotonin-synthesizing raphé/parapyramidal neurons (5-HT neurons) may function as sympathetic premotor neurons regulating sympathetic outflow to the cutaneous vascular bed. In the present study a genetically engineered pseudorabies virus (PRV) expressing green fluorescent protein (GFP) was injected into the rat tail. ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-9018-2
更新日期：2006-02-01 00:00:00
abstract：:This study was undertaken to evaluate whether chemical hypoxia-induced cell injury is a result of reactive oxygen species (ROS) generation, ATP depletion, mitochondrial permeability transition, and an increase in intracellular Ca2+, in A172 cells, a human glioma cell line. Chemical hypoxia was induced by incubating ce...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1024280429036
更新日期：2003-08-01 00:00:00
abstract：:Some crustacean axons remain functional for months after injury. This unusual property may require stress proteins synthesized by those neurons or provided to them by glial cells. To begin to explore this hypothesis, we examined the conditions that stimulated stress protein synthesis by crayfish CNS tissue in vitro. I...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00974871
更新日期：1991-05-01 00:00:00
abstract：:5- and 6-Hydroxydopamine, which we had earlier identified as naturally occurring amines in human urine, were quantified in Parkinson's patients treated with L-DOPA, Parkinson's patients whose treatment did not include L-DOPA and in age matched controls. Analysis was carried out by GC-MS of the ditrifluoromethylbenzoyl...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00978370
更新日期：1993-11-01 00:00:00
abstract：:Association of the candidate gene DLST with late-onset Alzheimer's disease (LOAD) risk has been suggested on the basis of case-control studies. This gene, located on chromosome 14q24.3, encodes a subunit of a mitochondrial component known to be defective in AD, the alpha-ketoglutarate dehydrogenase complex. Positive r...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000014833.54481.1d
更新日期：2004-03-01 00:00:00
abstract：:The characteristics and properties of the increase in cytosolic [Ca2+] that occurs in bovine adrenal medullary chromaffin cells on exposure to angiotensin 11 have been investigated. In fura-2 loaded cells exposure to a maximally effective concentration of angiotensin II (100 nM) caused a rapid, but transient increase ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1024987128090
更新日期：2003-09-01 00:00:00
abstract：:The putative dopamine D4 receptor protein in rat brain was labelled and quantified autoradiographically using two selective benzamides: [3H]YM-09151-2 which labels D2, D3 and D4 dopamine receptors and [3H]Raclopride which labels D2 and D3. The difference in densities of both ligands at saturable concentrations, show a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1027399408608
更新日期：1997-04-01 00:00:00
abstract：:We have developed a coupled Xenopus oocyte expression system for evaluating the functional effects of mutations in known or suspected adhesion molecules, which allows for a very rapid assessment of intercellular adhesion. As a model protein, we first used Protein zero (Po), an adhesion molecule that mediates self-adhe...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1010999622760
更新日期：2001-06-01 00:00:00
