Abstract：
:Cell surface β-Amyloid precursor protein (APP) is known to have a functional role in iron homeostasis through stabilising the iron export protein ferroportin (FPN). Mechanistic evidence of this role has previously only been provided through transcriptional or translational depletion of total APP levels. However, numerous post-translational modifications of APP are reported to regulate the location and trafficking of this protein to the cell surface. Stable overexpressing cell lines were generated that overexpressed APP with disrupted N-glycosylation (APPN467K and APPN496K) or ectodomain phosphorylation (APPS206A); sites selected for their proximity to the FPN binding site on the E2 domain of APP. We hypothesise that impaired N-glycosylation or phosphorylation of APP disrupts the functional location on the cell surface or binding to FPN to consequentially alter intracellular iron levels through impaired cell surface FPN stability. Outcomes confirm that these post-translational modifications are essential for the correct location of APP on the cell surface and highlight a novel mechanism by which the cell can modulate iron homeostasis. Further interrogation of other post-translational processes to APP is warranted in order to fully understand how each modification plays a role on regulating intracellular iron levels in health and disease.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Tsatsanis A,Dickens S,Kwok JCF,Wong BX,Duce JAdoi
10.1007/s11064-019-02747-ysubject
Has Abstractpub_date
2019-06-01 00:00:00pages
1367-1374issue
6eissn
0364-3190issn
1573-6903pii
10.1007/s11064-019-02747-yjournal_volume
44pub_type
杂志文章abstract：:The present investigation evaluates the anti-stress activity of risperidone (RIS) in the cold restraint stress (CRS) model and related stress pathways. Rats were pretreated with RIS (0.1 and 1.0 mg/kg) for 21 days before subjecting to CRS. Ultra low dose of RIS (ULD; 0.1 mg/kg) in contrast to higher dose (1.0 mg/kg) s...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0490-6
更新日期：2011-10-01 00:00:00
abstract：:The activity, properties, and developmental pattern of cysteine sulfinate transaminase (CSA-T) were studied in chick retina and compared with the activity, properties, and developmental pattern of glutamate oxaloacetate transaminase (GOT). Their optimum pH is identical whereas the effect of pyridoxal phosphate seems t...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964358
更新日期：1978-02-01 00:00:00
abstract：:The presynaptic dopamine (DA) D2 receptor-mediated regulation of ATP-sensitive potassium (K+ATP) channels was examined in slices of the rat caudate-putamen. When slices were incubated with the specific D2 receptor antagonist (-)-sulpiride (SLP), a concentration-dependent increase of extracellular DA release was observ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1026620316090
更新日期：2000-12-01 00:00:00
abstract：:The role of protein phosphorylation in catecholamine secretion from bovine adrenomedullary chromaffin cells was studied using different protein kinase inhibitors. Naphthalenesulfonamide derivatives as ML9 and ML7, more specific for the myosin light chain kinase, and the calmodulin antagonist W7 inhibited catecholamine...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969089
更新日期：1993-03-01 00:00:00
abstract：:Quantitative measurements were made of choline acetyltransferase (CAT) activity, acetylcholinesterase (AChE) activity and cholinergic muscarinic receptor binding ([3H]QNB) in eight areas of a cross-section of the rat medulla oblongata. A fourth cholinergic parameter, high-affinity choline uptake, was measured in three...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964389
更新日期：1981-05-01 00:00:00
abstract：:T-588(R(-)-1-(benzo(b)thiophen-5yl)-2-[2(N,N-diethylamino)ethoxy]ethanol hydrochloride) is a novel compound which has been shown to exhibit a wide range of neurotrophic effects both in vivo and in vitro. This compound can slow the motor deterioration of wobbler mouse motor neuron disease. However, it is not known whet...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1014884504879
更新日期：2002-03-01 00:00:00
abstract：:Protein kinase A (PKA) plays a crucial role in tau hyperphosphorylation, an early event of Alzheimer disease (AD), and 17beta-estradiol replacement in aging women forestalls the onset of AD. However, the role of estradiol in PKA-induced tau hyperphosphorylation is not known. Here, we investigated the effect of 17beta-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9638-4
更新日期：2008-09-01 00:00:00
abstract：:It is well known that the principal biomolecules involved in Alzheimer's disease (AD) are acetylcholinesterase (AChE), acetylcholine (ACh) and the amyloid beta peptide of 42 amino acid residues (Aβ42). ACh plays an important role in human memory and learning, but it is susceptible to hydrolysis by AChE, while the aggr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1400-5
更新日期：2014-11-01 00:00:00
abstract：:Enolase is a glycolytic enzyme whose amino acid sequence is highly conserved across a wide range of animal species. In mammals, enolase is known to be a dimeric protein composed of distinct but closely related subunits: alpha (non-neuronal), beta (muscle-specific), and gamma (neuron-specific). However, little informat...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00970738
更新日期：1995-08-01 00:00:00
abstract：:The putative dopamine D4 receptor protein in rat brain was labelled and quantified autoradiographically using two selective benzamides: [3H]YM-09151-2 which labels D2, D3 and D4 dopamine receptors and [3H]Raclopride which labels D2 and D3. The difference in densities of both ligands at saturable concentrations, show a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1027399408608
更新日期：1997-04-01 00:00:00
abstract：:Recent evidence reveals that prolactin gene expression (PRL-GE) in mammotropes occurs in pulses, but the molecular process(es) underlying this phenomenon remains unclear. Earlier, we have identified an E-box (E-box133) in the rat PRL promoter that binds several circadian elements and is critical for this dynamic proce...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1843-y
更新日期：2016-06-01 00:00:00
abstract：:Brains of Alzheimer disease patients in early stages of dementia contain an increased 24(S)-hydroxycholesterol (cerebrosterol)/cholesterol ratio when compared to controls. In this study, effects of amyloid beta peptides and of racemic 24-hydroxycholesterol were evaluated in vitro on undepleted or cholesterol-depleted ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9443-5
更新日期：2008-03-01 00:00:00
abstract：:The effect of 4-aminopyridine on [3H]acetylcholine release was studied in rat cerebral cortical synaptosomes in the presence of a several secretagogues that have different mechanisms of action. As found previously, 4-aminopyridine increased [3H]acetylcholine release in a concentration-dependent manner (5-10 mM); a hig...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969632
更新日期：1989-02-01 00:00:00
abstract：:Dual-specificity tyrosine-regulated kinase 2 (DYRK2), a protein kinase that phosphorylates its substrates on serine/threonine, is expressed in numerous human tumors, but little is known about its role in the pathophysiology of glioma. In this study, we made an effort to explore the expression and function in human gli...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2345-2
更新日期：2017-11-01 00:00:00
abstract：:The neuropathy associated with diabetes includes well documented impairment of axonal transport, a reduction in axon calibre and a reduced capacity for nerve regeneration. All of those aspects of nerve function rely on the integrity of the axonal cytoskeleton. Alterations in the axonal cytoskeleton in experimental dia...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022466624223
更新日期：1997-08-01 00:00:00
abstract：:Radioactive 125I seeds-based radiotherapy has achieved great success in treatment of human cancers. However, radioresistance and severe side effects badly limited its clinic application. Recently, chemoradiotherapy as a superior strategy has been rapidly developed and widely used in clinic. However, the underlying mec...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2547-2
更新日期：2018-07-01 00:00:00
abstract：:Ursolic acid (UA) is a plant derived compound which is also a component of the standard human diet. It possesses a wide range of pharmacological properties, i.e., antioxidant, anti-inflammatory, antimicrobial and antitumor, which have been used in folk medicine for centuries. Moreover, influence of UA on central nervo...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2505-z
更新日期：2018-05-01 00:00:00
abstract：:The potassium-stimulated release of acetylcholine, norepinephrine, serotonin, glutamate, and 4-aminobutyrate from superfused rat cortical slices was studied during hypoxia. A reduction in oxygen tensions from 603 +/- 6 to 22 +/- 2 mm Hg selectively altered the calcium-dependent efflux of these neurotransmitters, but d...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964800
更新日期：1984-08-01 00:00:00
abstract：:The stability of somatostatin (SS) in brain tissue was studied in human material obtained post-mortem and in the rat. In both human and rat brain, loss of SS was found to occur in tissue frozen to -70 degrees C. In the rat, this loss varied from 26 to 70 percent depending on the type of tissue processing used. These d...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973289
更新日期：1988-06-01 00:00:00
abstract：:Traumatic brain injury (TBI) initiates a series of neurochemical and signaling changes that could eventually lead to neuronal apoptosis. Recent studies indicated that mature neurons cell cycle re-enter played a crucial role in neuronal apoptosis. In this study, we identified that the chaperonin containing TCP-1, subun...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1683-1
更新日期：2015-09-01 00:00:00
abstract：:The incorporation of [gamma-32P]ATP into proteins of rat brain polyribosomes was studied in vitro. The effects of cyclic nucleotides, calcium, hemin, ACTH, GTP, and spermine were examined. The incorporation of phosphate into proteins increased with time and phosphatase activity was very low; thus, the extent of phosph...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973039
更新日期：1984-09-01 00:00:00
abstract：:Previous studies have shown that early life stress induced by maternal separation or non-handling can lead to behavioural deficits in rats and that these deficits can be alleviated by providing palatable cafeteria high-fat diet (HFD). In these studies we investigated the effects of maternal separation or non-handling ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-0985-4
更新日期：2013-04-01 00:00:00
abstract：:Guanine nucleotides (GN) have been implicated in many intracellular mechanisms. Extracellular actions, probably as glutamate receptor antagonists, have also been recently attributed to these compounds. GN may have a neuroprotective role by inhibiting excitotoxic events evoked by glutamate. Effects of extracellular GN ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007557600687
更新日期：2000-02-01 00:00:00
abstract：:The regional distribution of 2-amino-4-phosphonobutyrate (APB)/chloride-insensitive L-[3H]glutamate binding sites in the rat central nervous system was compared with that of APB/chloride-sensitive and with sodium-dependent binding sites. The distribution of APB-sensitive and APB-insensitive sites was not correlated, b...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963986
更新日期：1984-03-01 00:00:00
abstract：:Glutamine synthetase (GS) from the chick brain was purified to apparent homogeneity by ammonium sulfate fractionation followed by affinity chromatography, electrofocusing and Sephadex G-150 chromatography. The purified enzyme showed a single band on sodium dodecyl sulfate analysis in polyacrylamide gel. By sedimentati...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00970934
更新日期：1987-11-01 00:00:00
abstract：:Amyloidogenic proteins (Abeta peptide) in Alzheimer's disease (AD) and alpha-synuclein (alpha-Syn) in Parkinson's disease (PD) are typically soluble monomeric precursors, which undergo remarkable conformational changes and culminate in the form of aggregates in diseased condition. Overlap of clinical and neuropatholog...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9140-9
更新日期：2006-09-01 00:00:00
abstract：:Parkinson's disease (PD) is a neurodegenerative disease, with genetics and environment contributing to the disease onset. The limited pathological cognize of the disease restrained the approaches to improve the clinical treatment. Recently, studies showed that endoplasmic reticulum (ER) stress played an important role...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1922-0
更新日期：2016-08-01 00:00:00
abstract：:Trigeminal neuralgia (TN) is a type of chronic neuropathic pain that is caused by peripheral nerve lesions that result from various conditions, including the compression of vessels, tumors and viral infections. MicroRNAs (miRs) are increasingly recognized as potential regulators of neuropathic pain. Previous evidence ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02797-2
更新日期：2019-07-01 00:00:00
abstract：:The effects of 100microg melatonin injection at postnatal day 5 (PD 5) on the development of the central serotonergic systems in male and female rats were investigated. The contents of serotonin (5-HT) and 5-hydroxy-3-indolacetic acid (5-HIAA) were measured in several extrahypothalamic regions at 3, 10 and 42 weeks of...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007517524179
更新日期：2000-06-01 00:00:00
abstract：:We investigated the action of the new hypothalamic proline-rich peptide (PRP-1), normally produced by neurosecretory cells of hypothalamic nuclei (NPV and NSO), 3 and 4 weeks following rat sciatic nerve transection. The impulse activity flow of interneurons (IN) and motoneurons (MN) on stimulation of mixed (n. ischiad...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-2685-1
更新日期：2005-04-01 00:00:00
