Abstract：
:The incorporation of [gamma-32P]ATP into proteins of rat brain polyribosomes was studied in vitro. The effects of cyclic nucleotides, calcium, hemin, ACTH, GTP, and spermine were examined. The incorporation of phosphate into proteins increased with time and phosphatase activity was very low; thus, the extent of phosphorylation was predominantly a reflection of protein kinase activity. Phosphorylation of proteins was not sensitive to Ca2+ in the presence or absence of either calmodulin or phosphatidylserine. Phosphorylation was also unaffected by cyclic nucleotides in the absence of exogenous enzymes. However, addition of a cAMP-dependent protein kinase together with cAMP resulted in a stimulation of the incorporation of phosphate into 4 phosphoproteins (pp70, pp58, pp43, and pp32); phosphorylation of pp32 was completely dependent on the addition of the kinase. ACTH (1-24), (11-24), and spermine inhibited the endogenous phosphorylation of one protein band (pp30). The phosphorylation of this 30 kD band was also selectively increased by hemin (5 microM). Higher concentrations of hemin exerted an inhibitory effect on the majority of the phosphoproteins. Protein phosphatase activity was not influenced by ACTH or spermine. The specific inhibition of pp30 phosphorylation by ACTH or spermine is most probably explained by an interaction with a cyclic nucleotide- and Ca2+ -independent protein kinase.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Schrama LH,Frankena H,Edwards PM,Schotman Pdoi
10.1007/BF00973039subject
Has Abstractpub_date
1984-09-01 00:00:00pages
1267-81issue
9eissn
0364-3190issn
1573-6903journal_volume
9pub_type
杂志文章abstract：:Acidosis (pH 6.0) led to significant decrease in high-affinity choline uptake by rat brain synaptosomes. The effects persisted following pH readjustment (7.4) of the incubation medium, consisting of decrease in both Km and Vmax of the affinity system. pH readjustment coincided with synaptosomal leakage of lactate dehy...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969699
更新日期：1995-07-01 00:00:00
abstract：:Oxidative stress-induced neuronal cell death requires opening of the mitochondrial permeability transition pore. P53 mitochondrial translocation and association with Cyclophilin D (Cyp-D) is required for the pore opening. Here we tested this signaling axis in oxygen glucose deprivation (OGD)/re-oxygenation-induced in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-0968-5
更新日期：2013-04-01 00:00:00
abstract：:Ethylcholine mustard aziridinium ion (ECMA) was infused intracerebroventricularly (icv) to rats followed by measurement of two markers of presynaptic cholinergic neurons, choline acetyltransferase (ChAT) activity and high affinity choline transport (HAChT), in the hippocampus and cortex. Bilateral icv administration o...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00971589
更新日期：1988-08-01 00:00:00
abstract：:In vitro aggregation and fibrillization of synthetic amyloid beta-protein Abeta 1-40 was assessed in the conditioned media from rhabdomyosarcoma (CRL 1598, HTB 82, HTB 153, CCL 136), adenocarcinoma (CCL 218), neuroblastoma (SY5Y), and COS cells cultured in the absence and presence of 10% heat-inactivated fetal bovine ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1027379926976
更新日期：1997-02-01 00:00:00
abstract：:Trigeminal neuralgia (TN) is a type of chronic neuropathic pain that is caused by peripheral nerve lesions that result from various conditions, including the compression of vessels, tumors and viral infections. MicroRNAs (miRs) are increasingly recognized as potential regulators of neuropathic pain. Previous evidence ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02797-2
更新日期：2019-07-01 00:00:00
abstract：:A number of axonal proteins display changes in phosphorylation during goldfish optic nerve regeneration (Larrivee and Grafstein, 1989). (1) To determine whether the phosphorylation of these proteins was closely linked to their synthesis in the retinal ganglion cell body, cycloheximide was injected intraocularly into g...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965906
更新日期：1990-09-01 00:00:00
abstract：:Pyroptosis is a newly identified lytic form of programmed cell death which is characterized by plasma membrane blebbing and rupture. Pyroptosis occurs in cerebral ischemia injury, and contributes to the activation and secretion of the inflammatory cytokines interleukin (IL)-1β, IL-18, and IL-6. Previous reports have f...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02935-w
更新日期：2020-02-01 00:00:00
abstract：:Astrocytes occupy a strategic position in the brain where they can act as an interface between neurones and blood vessels, and neurones and the cerebro-spinal fluid. This location is ideal for functioning as interoceptors, as they may sense changes in brain microenvironment and contribute to the adaptive homeostatic r...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-015-1562-9
更新日期：2015-12-01 00:00:00
abstract：:We found that chronic lithium diet affects the sensitivity of neuroleptic receptors and the content of amino acids in the brain, and that the changes in adult animals differ from those in young rats. Pregnant rats were kept on lithium diet (pellets with 0.21% Li2CO3 and 0.21% NaCl) during the gestation period and the ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964703
更新日期：1983-05-01 00:00:00
abstract：:Parkinson's disease (PD) is a severe neurodegenerative disease characterized by selective loss of dopaminergic neurons, which reduces quality of life of patients and poses a heavy burden to the society. The pathological mechanism of PD remains unclear, and increasing efforts are aimed to solve this problem. MiRNAs are...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03166-0
更新日期：2020-11-12 00:00:00
abstract：:The neuropathy associated with diabetes includes well documented impairment of axonal transport, a reduction in axon calibre and a reduced capacity for nerve regeneration. All of those aspects of nerve function rely on the integrity of the axonal cytoskeleton. Alterations in the axonal cytoskeleton in experimental dia...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022466624223
更新日期：1997-08-01 00:00:00
abstract：:The SAM strains, a group of related inbred strains consisting of senescence-prone inbred strains (SAMP) and senescence-resistant inbred strains (SAMR), have been successfully developed by selective inbreeding of the AKR/J strain of mice donated by the Jackson laboratory in 1968. The characteristic feature of aging com...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-009-9922-y
更新日期：2009-04-01 00:00:00
abstract：:Microglia, the resident immune cells of the CNS, are primary regulators of the neuroimmune response to injury. Type I interferons (IFNs), including the IFNαs and IFNβ, are key cytokines in the innate immune system. Their activity is implicated in the regulation of microglial function both during development and in res...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2307-8
更新日期：2017-09-01 00:00:00
abstract：:The major breakthrough discovery of enkephalins as endogenous opiates led our attempts to determine their inactivation mechanisms. Because the NH2-terminal tyrosine is absolutely necessary for the neuropeptides to exert analgesic effects, and aminopeptidase activities are extraordinarily high in the brain, a specific ...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-007-9356-3
更新日期：2007-12-01 00:00:00
abstract：:There is increasing evidence that proteins normally involved in the cell cycle play a role in the regulation of neuronal apoptotic death following various insults. However, it is not clear if the same mechanisms regulate cell death of oligodendrocytes as well. In this study, we investigated the mechanism of ceramide-i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000021236.32785.37
更新日期：2004-05-01 00:00:00
abstract：:Hydroxysafflor yellow A (HSYA) is a component of the flower Carthamus tinctorius L. that elicits neuroprotective effects in vivo and in vitro. The purpose of this study was to investigate pharmacological properties of HSYA on neurotoxicity of glutamate in primary cultured rat cortical neurons along with its possible m...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0191-6
更新日期：2010-09-01 00:00:00
abstract：:Gelsolin is an actin regulatory protein that generally distributed in a wide variety of body tissues, especially the brain tissues and cerebrospinal fluid. In this study we found that lumbar CSF-gelsolin concentrations markedly decreased in epileptic patients by enzyme linked immunosorbent assay. In order to help judg...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0549-4
更新日期：2011-12-01 00:00:00
abstract：:In human pathology little is known about the activating enzymes for fatty acids of different carbon chain length. In order to have a better insight into disorders of lipid metabolism in human skeletal muscle, we studied the distribution of acyl-CoA synthetases in muscular subcellular fractions. We find that in muscle ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964696
更新日期：1983-05-01 00:00:00
abstract：:Lewy bodies and Lewy neurites in the brain constitute the main histopathological features of Parkinson's disease (PD) and dementia with Lewy bodies. They comprise amyloid-like fibrils composed of α-synuclein (αS), a small protein (~14 kDa). Because the aggregation of αS in the brain has been implicated as a critical s...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2382-x
更新日期：2017-12-01 00:00:00
abstract：:We analyzed two disease model groups with rats infected by Japanese encephalitis virus (JEV), a 90-day group and a 180-day group after JEV infection. The time measured by the modified pole test showed that motor activities in these two groups were slower than those of age-matched control groups. Striatal dopamine (DA)...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9197-5
更新日期：2006-12-01 00:00:00
abstract：:Eclalbasaponin II derived from Eclipta prostrata L. (Asteraceae) has been reported to have anti-fibrotic, anti-bacterial and autophagic activities, but its effect on cognitive function has not been investigated. We studied the effect of eclalbasaponin II on cholinergic blockade-induced memory impairment in mice using ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2430-6
更新日期：2018-02-01 00:00:00
abstract：:Effects of methylmercury (MetHg) on the specific [3H]flunitrazepam binding were studied in rat cortical and cerebellar P2-fractions in vitro. MetHg did not affect significantly the specific [3H]flunitrazepam binding in unwashed P2-fraction but increased it marginally (by 16%) at 100 microM in washed P2-fraction, in bo...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01705532
更新日期：1995-06-01 00:00:00
abstract：:The K-stimulated release of [3H]ACh from rat midbrain slices prelabeled by incubation with [3H]choline was dependent on extracellular Ca. Phenobarbital inhibited the K-stimulated [3H]ACh release and the IC50 was equal to that found for K-stimulated endogenous ACh release. These results support the suggestion that barb...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964710
更新日期：1980-07-01 00:00:00
abstract：:5- and 6-Hydroxydopamine, which we had earlier identified as naturally occurring amines in human urine, were quantified in Parkinson's patients treated with L-DOPA, Parkinson's patients whose treatment did not include L-DOPA and in age matched controls. Analysis was carried out by GC-MS of the ditrifluoromethylbenzoyl...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00978370
更新日期：1993-11-01 00:00:00
abstract：:We have attempted to show eventual modifications in the brain protein synthesis apparatus of rat during the first three weeks after birth. Through this time we noted a steady decrease (about 60%) in the free polysomes, when expressed relative to tissue weight. This decrease does not correlate with changes in the polys...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966130
更新日期：1986-09-01 00:00:00
abstract：:Disruptive effects of calcium upon neurofilaments and glial filaments were studied in white matter of rat optic nerve and spinal cord and in rat peripheral nerve. Filament ultrastructure and tissue protein composition were compared following a calcium influx into excised tissues. A calcium influx was induced by freeze...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964040
更新日期：1981-03-01 00:00:00
abstract：:Oxidative stress is known to induce cell death in a wide variety of cell types, apparently by modulating intracellular signaling pathways. Activation of extracellular signal-regulated kinase (ERK) in oxidative stress remains controversial. In some cellular systems, the ERK activation is associated with protection agai...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-2449-y
更新日期：2005-02-01 00:00:00
abstract：:Several studies showed that the up-regulation of glial glutamate transporter-1 (GLT-1) participates in the acquisition of brain ischemic tolerance induced by cerebral ischemic preconditioning or ceftriaxone pretreatment in rats. To explore whether GLT-1 plays a role in the acquisition of brain ischemic tolerance induc...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0639-3
更新日期：2012-03-01 00:00:00
abstract：:Parkinson's disease (PD) is characterized by the presence of insoluble protein clusters containing α-synuclein. Impairment of mitochondria, endoplasmic reticulum, autophagy and intracellular trafficking proper function has been suggested to be caused by α-synuclein toxicity, which is also associated with the higher le...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-018-2673-x
更新日期：2018-12-01 00:00:00
abstract：:The monoclonal antibody 14E immunocytochemically stains the nuclear membrane of oligodendrocytes but not myelin in tissue sections of adult normal human white matter. The nuclear membranes of Schwann cells in human peripheral nerve and cerebellar Bergmann glia were also visualized with this antibody. In actively demye...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00993270
更新日期：1992-09-01 00:00:00
