Abstract：
:A number of axonal proteins display changes in phosphorylation during goldfish optic nerve regeneration (Larrivee and Grafstein, 1989). (1) To determine whether the phosphorylation of these proteins was closely linked to their synthesis in the retinal ganglion cell body, cycloheximide was injected intraocularly into goldfish whose optic nerves had been regenerating for 3 weeks. Cycloheximide reduced the incorporation of [3H]proline and 32P orthophosphate into total nerve protein by 84% and 46%, respectively. Of the 20 individual proteins examined, 17 contained less than 15% of the [3H]proline label measured in corresponding controls, whereas 18 proteins contained 50% or more of the 32P label, suggesting that phosphorylation was largely independent of synthesis. (2) To determine whether the proteins were phosphorylated in the ganglion cell axons, axonal transport of proteins was blocked by intraocular injection of vincristine. Vincristine reduced [3H]proline labeling of total protein by 88% and 32P labeling by 49%. Among the individual proteins [3H]proline labeling was reduced by 90% or more in 18 cases but 32P labeling was reduced only by 50% or less. (3) When 32P was injected into the cranial cavity near the ends of the optic axons, all of the phosphoproteins were labeled more intensely in the optic tract than in the optic nerve. These results suggest that most of the major phosphoproteins that undergo changes in phosphorylation in the course of regeneration are phosphorylated in the optic axons.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Larrivee Ddoi
10.1007/BF00965906subject
Has Abstractpub_date
1990-09-01 00:00:00pages
875-80issue
9eissn
0364-3190issn
1573-6903journal_volume
15pub_type
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journal_title：Neurochemical research
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更新日期：1984-07-01 00:00:00
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journal_title：Neurochemical research
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更新日期：2008-02-01 00:00:00
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更新日期：2014-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF02527668
更新日期：1996-01-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969699
更新日期：1995-07-01 00:00:00
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更新日期：2016-05-01 00:00:00
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更新日期：2019-06-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：1981-11-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00995551
更新日期：1995-09-01 00:00:00
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doi：10.1007/BF00965316
更新日期：1986-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00967320
更新日期：1994-04-01 00:00:00
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更新日期：2014-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00966130
更新日期：1986-09-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00967242
更新日期：1993-04-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966598
更新日期：1991-10-01 00:00:00
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更新日期：2007-08-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966781
更新日期：1993-06-01 00:00:00
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更新日期：2003-12-01 00:00:00
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更新日期：2011-10-01 00:00:00
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更新日期：2000-12-01 00:00:00
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doi：10.1007/s11064-005-2754-5
更新日期：2005-05-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966825
更新日期：1992-10-01 00:00:00
abstract：:Docosahexaenoic acid (DHA) can alleviate cerebral ischemia/reperfusion injury by reducing blood-brain barrier permeability and maintaining its integrity, accompanied by an increased Ang-1/Ang-2 ratio; however, the underlying mechanisms of these effects remain unclear. Src-suppressed C kinase substrates (SSeCKS), a sub...
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pub_type： 杂志文章
doi：10.1007/s11064-019-02915-0
更新日期：2020-02-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：2016-10-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1603-4
更新日期：2015-07-01 00:00:00
abstract：:Convulsant doses of penicillin and elevated ambient pressure of 41 bar enhance the excitability of neurons. Their effects have been studied in neostriatal tissue with methods allowing differentiation between transmitter and metabolic glutamate pools. Levels of glutamate (Glu), glutamine (Gln), aspartate (Asp); gamma-a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965621
更新日期：1989-11-01 00:00:00
abstract：:The phospholipid requirement for Ca2+-stimulated, Mg2+-dependent ATP hydrolysis (Ca2+/Mg2+-ATPase) and Mg2+-stimulated ATP hydrolysis (Mg2+-ATPase) in rat brain synaptosomal membranes was studied employing partial delipidation of the membranes with phospholipase A2 (Hog pancreas), phospholipase C (Bacillus cereus) and...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966224
更新日期：1986-10-01 00:00:00
