Abstract:
:Suspensions of rat brain microsomes, synaptosomes, and synaptic vesicles were able to convert adenosine to inosine by means of adenosine deaminase. Isosbestic points of this transformation, at 222, 250 and 281 nm, remained unchanged with time-course. This fact suggests that adenosine deaminase (ADA, E.C. 3.5.4.4) is located on the surface of the vesicles whereas purine nucleoside phosphorylase (PNP, E.C. 2.1.2.4) is located inside the vesicles. Kinetic parameters of the particulate 5'-nucleotidase (5'N, E.C. 3.1.3.5) and adenosine deaminase were analogous to those of the cytosolic enzymes. These results suggest that soluble and particulate enzymes represent different pools of the same molecular species.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Centelles JJ,Franco R,Canela EI,Bozal Jdoi
10.1007/BF00965316subject
Has Abstractpub_date
1986-04-01 00:00:00pages
471-9issue
4eissn
0364-3190issn
1573-6903journal_volume
11pub_type
杂志文章abstract::This work evaluated the in vitro effect of thiazolidin-4-ones on the activity of AChE (total and isoforms) isolated from the cerebral cortex, hippocampus, and lymphocytes. Kinetic parameters were evaluated and molecular docking was performed. Our results showed that thiazolidinones derived from 4-(methylthio)benzaldeh...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-019-02929-8
更新日期:2020-02-01 00:00:00
abstract::The present study investigated the expression patterns of glial cells and interleukin-1beta (IL-1beta) in the rat spinal cord after a surgical incision, which is closely related with clinical postoperative pain. Microglia and astrocytes became activated in the spinal cord following incision. Real-time polymerase chain...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-9032-4
更新日期:2006-03-01 00:00:00
abstract::The effect of L-glutamate (Glu) and its structural analogs N-methyl-D-aspartate (NMDA), kainate (KA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA), on the activation of p42 mitogen activated protein kinase (MAPK) was examined in cultured chick radial glia cells, namely retinal Muller cells and cerebe...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027345808746
更新日期:1997-06-01 00:00:00
abstract::Studies on primary astrocytes cultured in vitro have shown that process formation involves changes in cytoskeletal proteins and release of tension on the substratum. Actin filament reorganization has previously been found to be the major cytoskeletal change occurring during process formation. These changes are relativ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022413718447
更新日期:1998-03-01 00:00:00
abstract::Subunit-specific antibodies to all the gamma subunit isoforms described in mammalian brain (gamma(1), gamma(2S), gamma(2L), and gamma(3) have been made. The proportion of GABA(A) receptors containing each gamma subunit isoform in various brain regions has been determined by quantitative immunoprecipitation. In all tes...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02529132
更新日期:1996-02-01 00:00:00
abstract::Ursolic acid (UA) is a plant derived compound which is also a component of the standard human diet. It possesses a wide range of pharmacological properties, i.e., antioxidant, anti-inflammatory, antimicrobial and antitumor, which have been used in folk medicine for centuries. Moreover, influence of UA on central nervo...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2505-z
更新日期:2018-05-01 00:00:00
abstract::The kinetics and specificity of taurine and beta-alanine uptake were studied in primary cultures of rat astrocytes under identical experimental conditions. The uptake consisted of nonsaturable penetration and saturable high-affinity transport that was strictly sodium dependent. The cells accumulated taurine more effec...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967969
更新日期:1986-02-01 00:00:00
abstract::Chemokines are small secreted proteins that are essential for the recruitment and activation of specific leukocyte subsets at sites of inflammation and for the development and homeostasis of lymphoid and nonlymphoid tissues. During the past decade, chemokines and their receptors have also emerged as key signaling mole...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/b:nere.0000021246.96864.89
更新日期:2004-05-01 00:00:00
abstract::Cyclin-dependent kinase 5 (cdk5) is a proline-directed serine/threonine kinase that is activated mostly by association with its activators, p35 and p39. Initially projected as a neuron-specific kinase, cdk5 is expressed ubiquitously and its kinase activity solely depends on the presence of its activators, which are al...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-008-9885-4
更新日期:2009-06-01 00:00:00
abstract::Neuronal excitation leads to an increase of the extracellular K(+) concentration ([K(+)]o) in brain. This increase has at least two energy-consuming consequences: (1) a depolarization-mediated change in intracellular pH (pHi) in astrocytes due to depolarization-mediated increased activity of the acid-extruding Na(+)/b...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1149-2
更新日期:2013-11-01 00:00:00
abstract::Experimentalists would like to perform large-scale expression analysis on samples drawn from small cell populations and even single cells. Amplification of the source material is required to assay such small samples under all existing array technologies. In this report we consider the very different theoretical proper...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020917223962
更新日期:2002-10-01 00:00:00
abstract::The MAO inhibitor phenelzine (PLZ) at a dose of 25 mg/kg does not affect the behavior of rats. In contrast, the equivalent dose of a deuterated analog (alpha, alpha, beta, beta-tetradeutero-PLZ, d4PLZ) elicits a biphasic behavioral syndrome in rats. In an attempt to correlate changes in cerebral monoamines with behavi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973322
更新日期:1988-02-01 00:00:00
abstract::We have attempted to show eventual modifications in the brain protein synthesis apparatus of rat during the first three weeks after birth. Through this time we noted a steady decrease (about 60%) in the free polysomes, when expressed relative to tissue weight. This decrease does not correlate with changes in the polys...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966130
更新日期:1986-09-01 00:00:00
abstract::Any spinal cord injury carries the potential for persistent disability affecting motor, sensory and autonomic functions. To prevent this outcome, it is highly desirable to block a chain of deleterious reactions developing in the spinal areas immediately around the primary lesion. Thus, early timing of pharmacological ...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-017-2459-6
更新日期:2019-01-01 00:00:00
abstract::Danhong injection, a Chinese Materia Medica standardized product extracted from Radix Salviae miltiorrhizae and Flos Carthami tinctorii, is used extensively for the treatment of cerebrovascular diseases such as acutely cerebral infarction in clinic. In this study, we further investigated the mechanisms of Danhong inje...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1384-1
更新日期:2014-09-01 00:00:00
abstract::Using sodium (NaN3)-induced anoxia plus aglycaemia as a model of chemically-induced ischemia, we have characterized the endogenous release of excitatory and inhibitory amino acids from superfused hippocampal slices. Chemical ischemia produced an azide (1-30 mM) dose-dependent increase in the efflux of glutamate, aspar...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1011094728469
更新日期:2001-02-01 00:00:00
abstract::Glutamate dehydrogenase (GDH) is a homohexameric enzyme that catalyzes the reversible oxidative deamination of L-glutamate to 2-oxoglutarate. Only in the animal kingdom is this enzyme heavily allosterically regulated by a wide array of metabolites. The major activators are ADP and leucine and inhibitors include GTP, p...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-013-1173-2
更新日期:2014-01-01 00:00:00
abstract::The hypothalamic paraventricular nucleus (PVN) is a key site for regulating neuroendocrine functions in the magnocellular part and autonomic activities in the parvocellular part. Its anatomical proximity to the third ventricle could be a good target for intrathecal injection of baclofen. We investigated the correlatio...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0621-0
更新日期:2012-02-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is an adult-onset, irreversible neurodegenerative disease that leads to progressive paralysis and inevitable death 3-5 years after diagnosis. The mechanisms underlying this process remain unknown, but new evidence indicates that accumulating levels of D-serine result from the downre...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2131-6
更新日期:2017-04-01 00:00:00
abstract::A role for protein phosphorylation in the process of neurite outgrowth has been inferred from many studies of the effects of protein kinase inhibitors and activators on cultured neurotumor cells and primary neuronal cells from developing brain or ganglia. Here we re-examine this issue, using a culture system derived f...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1021916509858
更新日期:1997-10-01 00:00:00
abstract::A concise discussion of the role of different geometrical conformational states in the process of self-assembling of gangliosides is given. The report focuses on the effects of the geometrical variations occurring in the head group region of gangliosides as reflected on the geometrical properties of the whole assembly...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1020255529125
更新日期:2002-08-01 00:00:00
abstract::The preventive effect of dietary taurine supplementation on glial alterations in retina of streptozotocin-induced diabetic rats was examined in this study. Blood glucose content, content of taurine, glutamate and
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9763-0
更新日期:2009-02-01 00:00:00
abstract::It is reported that chitinase1 increases in Alzheimer's disease (AD). However, the alteration of chitinase1 in the progress of AD is still unclear. Thus, we designed the present study to detect chitinase1 level in different stages of APP/PS1 double transgenic mice. Experimental models were APP/PS1 double transgenic mi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1874-4
更新日期:2016-07-01 00:00:00
abstract::The synthetic Liver X receptor (LXR) activator T0901317 has been reported to exert neuroprotective effect in Alzheimer's disease, but the relationship between LXR activation and beta-site amyloid precursor protein cleaving enzyme 1 (BACE-1) remains uncertain. This study investigated the effect of T0901317 on membrane ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0513-3
更新日期:2011-10-01 00:00:00
abstract::In an effort to develop an animal model of hyperpipecolatemia, the uptake of pipecolic acid (PA) in the brain and changes of PA levels in serum following administration of D,L-PA were studied in the mouse using a new sensitive HPLC-EC method. Following i.p. injections (250 mg/kg) to adult male mice, the brain concentr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964981
更新日期:1985-10-01 00:00:00
abstract::Neuroglycan C (NGC) is a transmembrane-type chondroitin sulfate proteoglycan that promotes neurite outgrowth. To identify the ligand of NGC, we applied a detergent-solubilized membrane fraction of fetal rat brains to an NGC-immobilized affinity column. Several proteins were eluted from the column including an 18 kDa-b...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0164-9
更新日期:2010-08-01 00:00:00
abstract::The immediate and long-term effects of the selective serotonergic neurotoxin 5,7-dihydroxytryptamine (5,7-DHT) on rat striatal serotonergic neurons were examined after its intracerebroventricular administration using in vivo voltammetry. Extracellular concentration of 5-hydroxyindoles increased immediately following i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022460216822
更新日期:1998-01-01 00:00:00
abstract::Metabolic modeling of dynamic (13)C labeling curves during infusion of (13)C-labeled substrates allows quantitative measurements of metabolic rates in vivo. However metabolic modeling studies performed in the brain to date have only modeled time courses of total isotopic enrichment at individual carbon positions (posi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0782-5
更新日期:2012-11-01 00:00:00
abstract::Probenecid inhibits the transport of the small monocarboxylic acids lactate and propionate from blood to brain but does not affect the transport of 5HIAA or HVA. Neither lactate, 5HIAA, HVA, nor probenecid itself inhibits probenecid uptake into brain from blood and neither lactate nor 5HIAA itself inhibits 5HIAA uptak...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965898
更新日期:1982-10-01 00:00:00
abstract::Mitochondrial division inhibitor 1 (Mdivi-1) is the most effective pharmacological inhibitor of mitochondrial fission. Spinal cord injury (SCI) is a common and serious trauma, which lacks efficient treatment. This study aimed to detect the role of Mdivi-1 in neuronal injury and its underlying mechanism after acute SCI...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1604-3
更新日期:2015-07-01 00:00:00