Abstract:
:Experimentalists would like to perform large-scale expression analysis on samples drawn from small cell populations and even single cells. Amplification of the source material is required to assay such small samples under all existing array technologies. In this report we consider the very different theoretical properties of linear and PCR-based amplification. A suggestion is made concerning a hybrid amplification strategy incorporating the advantages offered by both approaches.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Shaw CAdoi
10.1023/a:1020917223962keywords:
subject
Has Abstractpub_date
2002-10-01 00:00:00pages
1123-31issue
10eissn
0364-3190issn
1573-6903journal_volume
27pub_type
杂志文章abstract::During neural activity, neurotransmitters released at synapses reach neighbouring cells, such as astrocytes. These get excited via numerous mechanisms, including the G protein coupled receptors that regulate the cytosolic concentration of second messengers, such as Ca(2+) and cAMP. The stimulation of these pathways le...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-015-1545-x
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abstract::Schwann cell biosynthesis of glycolipids was studied by in vitro incorporation of [3H]galactose into neonatal rat sciatic nerves before and after endoneurial explant culture and in culture of purified Schwann cells. In neonatal nerves prior to culture, [3H]galactose was actively incorporated into galactocerebrosides (...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968672
更新日期:1990-03-01 00:00:00
abstract::Remyelination promoting human IgMs effectively increase the number of myelinated axons in animal models of multiple sclerosis. Hence, they ultimately stimulate myelin production by oligodendrocytes (OLs); however, their exact mechanism of action remains to be elucidated, and in particular, it remains unclear whether t...
journal_title:Neurochemical research
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964040
更新日期:1981-03-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1014898421330
更新日期:2002-02-01 00:00:00
abstract::Sleep disorders negatively affect cognition and health. Recent evidence has indicated that chromatin remodeling via histone acetylation regulates cognitive function. This study aimed to investigate the possible roles of histone acetylation in sleep deprivation (SD)-induced cognitive impairment. Results of the Morris w...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1937-6
更新日期:2016-09-01 00:00:00
abstract::Glutamine synthetase (GS) from the chick brain was purified to apparent homogeneity by ammonium sulfate fractionation followed by affinity chromatography, electrofocusing and Sephadex G-150 chromatography. The purified enzyme showed a single band on sodium dodecyl sulfate analysis in polyacrylamide gel. By sedimentati...
journal_title:Neurochemical research
pub_type: 杂志文章
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journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-016-2030-x
更新日期:2017-03-01 00:00:00
abstract::The overlapping distribution of opioid and cholecystokinin (CCK) peptides and their receptors (mu and delta opioid receptors; CCK-A and CCK-B receptors) in the central nervous system have led to a large number of studies aimed at clarifying the functional relationships between these two neuropeptides. Most of the phar...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
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abstract::Bovine brain synaptic membranes which were frozen and then extensively washed showed low affinity [3H]muscimol binding. These membranes contained GABA and calmodulin, apparently tightly bound within the membrane fraction. Membranes which were additionally treated with the detergent Triton X-100 showed high affinity [3...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970756
更新日期:1988-09-01 00:00:00
abstract::It is now established that (1) concentrations of 2-phenylethylamine (PEA) are greatly increased in brain following administration of monoamine oxidase inhibitor (MAOI) antidepressants; (2) PEA is a metabolite of the MAOI antidepressant phenelzine; and (3) PEA may be a neuromodulator of catecholamine activity. On the b...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00966762
更新日期:1993-09-01 00:00:00
abstract::The neurotoxic effects of aluminum are generally associated with reduced antioxidant capacity, increased oxidative stress and apoptosis, which lead to the induction of neurodegenerative processes. Curcumin has a lipophilic polyphenol character and effects of antioxidant and anti-apoptotic. The present study was undert...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-019-02839-9
更新日期:2019-08-01 00:00:00
abstract::Microdissection of selected regions of central nervous system (CNS) has provided the basis of modern chemoarchitectonics. Laser microdissection is a modern variant of the "Palkovits punch" technique and used together with gene array analysis has revolutionalized CNS molecular analysis. Here we describe the use of such...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-9008-4
更新日期:2006-02-01 00:00:00
abstract::The activation of caspase-3 is considered to be a reliable marker for apoptotic cell death, and a 120-kDa fragment of αII-spectrin is generated by caspase-3 mediated cleavage of this structural protein. In the present study, we compared cleaved αII-spectrin (120-kDa) and cleaved caspase-3-immunoreactive cells and thei...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0633-9
更新日期:2012-03-01 00:00:00
abstract::Oxidative stress can induce neuronal apoptosis via the production of superoxide and hydroxyl radicals. This process is as a major pathogenic mechanism in neurodegenerative disorders. In this study, we aimed to clarify whether theaflavins protect PC12 cells from oxidative stress damage induced by H2O2. A cell model of ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2069-8
更新日期:2016-12-01 00:00:00
abstract::Parkinson's disease (PD) is characterized by the presence of insoluble protein clusters containing α-synuclein. Impairment of mitochondria, endoplasmic reticulum, autophagy and intracellular trafficking proper function has been suggested to be caused by α-synuclein toxicity, which is also associated with the higher le...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-018-2673-x
更新日期:2018-12-01 00:00:00
abstract::The activity, properties, and developmental pattern of cysteine sulfinate transaminase (CSA-T) were studied in chick retina and compared with the activity, properties, and developmental pattern of glutamate oxaloacetate transaminase (GOT). Their optimum pH is identical whereas the effect of pyridoxal phosphate seems t...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964358
更新日期:1978-02-01 00:00:00
abstract::Apoptotic cell death pathways have been implicated in acute brain injuries, including cerebral ischemia, brain trauma, and spinal cord injury, and in chronic neurodegenerative diseases. Experimental ischemia and reperfusion models, such as transient focal/global ischemia in rodents, have been thoroughly studied and su...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-004-6869-x
更新日期:2004-11-01 00:00:00
abstract::Exploring the neurochemical continuum between frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) with respect to monoamines and kynurenines in cerebrospinal fluid (CSF) and serum, may be useful to identify possible new research/therapeutic targets. Hence, we analysed monoamines and kynurenines in CS...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03002-5
更新日期:2020-05-01 00:00:00
abstract::This study examined changes in neuropeptide Y (NPY) Y2 receptor binding in the brains of C57BL/6 mice in response to different levels of high-fat diets via three dietary intervention methods: high-fat diet, switching from high- to low-fat diet and finally, energy restricted high-fat diet. Forty-five C57Bl/6 male mice ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9661-5
更新日期:2008-09-01 00:00:00
abstract::Parkinson's disease (PD) is a common progressive neurodegenerative disorder, for which at present no causal treatment is available. On the understanding that the causes of PD are mainly oxidative stress and mitochondrial dysfunction, antioxidants and other drugs are expected to be used. In the present study, we demons...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-0046-1
更新日期:2010-02-01 00:00:00
abstract::The aim of present study was to determine the effect of 3',4'-dihydroxyflavonol (DiOHF) on lipid peroxidation in experimental brain ischemia-reperfusion in rats. Present study was performed on the 34 male Wistar-albino rats, weigth 350-400 g. Experiment groups were designed as 1-Sham; 2-Ischemia-reperfusion; animal we...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1889-x
更新日期:2016-07-01 00:00:00
abstract::Regional and developmental changes in metabolite concentrations were measured by 1H NMR spectroscopy and HPLC of perchloric acid extracts from rat brain and eye. The highest concentrations of N-acetylaspartate were found in grey matter as opposed to white matter with concentration increasing with age from neonate to a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02532417
更新日期:1996-09-01 00:00:00
abstract::Our previous studies have shown that three sigma (sigma) receptor ligands, (+)-N-allylnormetazocine ((+)-SKF-10,047), (+/-)-pentazocine and 1,3-di(2-tolyl)guanidine (DTG) differently regulated the dopamine (DA) transmission in the rat brain. In the present study, we attempted to clarify the role of sigma 1 receptor su...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027361101419
更新日期:1997-09-01 00:00:00
abstract::Protocatechuic acid (PA), a major metabolite of anthocyanins, has been reported to possess antioxidant and anti-inflammatory activities. However, the effects of PA on LPS-induced inflammatory responses in microglia have not been reported. The aim of this study was to investigate the anti-inflammatory effects and molec...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1646-6
更新日期:2015-08-01 00:00:00
abstract::Changes in serotonin (5-HT) and 5-hydroxy indole acetic acid (5-HIAA), its major metabolite, in cerebral cortex, corpus striatum and hippocampus were investigated at 10th and 21st days of chronic ethanol ingestion in Wistar rats. Ethanol (7.2% v/v) was given to rats in a modified liquid diet. Biochemical analysis was ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1007579705230
更新日期:2000-02-01 00:00:00
abstract::A hemagglutinating activity was detected in a synaptic vesicle-enriched fraction prepared from adult rat brain, using trypsinized glutaraldehyde-fixed rabbit erythrocytes. The specific activity of the fraction, in two series of experiments, was 7.5 and 16-fold higher than in the other subcellular fractions. The activi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971856
更新日期:1988-01-01 00:00:00
abstract::Aging is accompanied by the loss of memory and cognitive functions. The extracellular signal-regulated kinase (ERK) pathway has been shown to play an essential role in synaptic plasticity and memory. Although a reduction in basal ERK1/2 activity has been found in the cerebral cortex in aged rats, changes in ERK1/2 mRN...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1024948532633
更新日期:2003-09-01 00:00:00
abstract::Acetylcholinesterase (AChE) inhibition has been described as the main mechanism of organophosphate (OP)-evoked toxicity. OPs represent a human health threat, because chronic exposure to low doses can damage the developing brain, and acute exposure can produce long-lasting damage to adult brains, despite post-exposure ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1548-7
更新日期:2015-10-01 00:00:00
abstract::Parkinson's disease (PD) is a severe neurodegenerative disease characterized by selective loss of dopaminergic neurons, which reduces quality of life of patients and poses a heavy burden to the society. The pathological mechanism of PD remains unclear, and increasing efforts are aimed to solve this problem. MiRNAs are...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03166-0
更新日期:2020-11-12 00:00:00