Excitable Astrocytes: Ca(2+)- and cAMP-Regulated Exocytosis.

abstract：:A simple, rapid method for determining depolarization-induced 45Ca influx into synaptosomes is described. Synaptosomes which had been depolarized in the presence of 45Ca were applied to a small column of Chelex-100 resin to separate free Ca2+ from that taken up by the tissue. Approximately 70% of the synaptosomal prot...

journal_title：Neurochemical research

authors： Michaelis ML,Michaelis EK,Simpson AE

更新日期：1982-02-01 00:00:00

abstract：:Endoplasmic reticulum (ER) and mitochondria are intracellular organelles and their interactions are directly involved in different processes such as Ca(2+) signaling in cell survival and death mechanisms. Bcl-2 is an anti-apoptotic protein intrinsically related to ER and mitochondria, modulating Ca(2+) content in thes...

journal_title：Neurochemical research

authors： Hirata H,Lopes GS,Jurkiewicz A,Garcez-do-Carmo L,Smaili SS

更新日期：2012-02-01 00:00:00

abstract：:The concentrations of dopamine (DA), 5-hydroxytryptamine (5-HT) and noradrenaline (NA) in the rat vas deferens divided in eight or four sections were determined by high performance liquid chromatography with electrochemical detection. Dopamine and NA had the same regional distribution; their concentrations were maxima...

journal_title：Neurochemical research

authors： Celuch SM,Sloley BD

更新日期：1988-10-01 00:00:00

abstract：:The diversification of neuronal subtypes during corticogenesis is fundamental to the establishment of the complex cortical structure. Although subtype specification has been assumed to occur in neural progenitor cells, increasing evidence has begun to reveal the plasticity of subtype determination in immature neurons....

journal_title：Neurochemical research

authors： Oishi K,Nakajima K

更新日期：2018-01-01 00:00:00

abstract：:Consumption of fifteen percent alcohol, during gestation did not cause any decrease in the total calorie or fluid intake of the rats maintained on normal dietary regimen. However, the alcohol consumption by gestating mothers resulted in a decreased contents of both DNA and protein in the CNS of the in utero alcohol ex...

journal_title：Neurochemical research

authors： Prasad VV

更新日期：1989-11-01 00:00:00

abstract：:Glycogen is the main storage form of glucose in the brain. In contrast with previous beliefs, brain glycogen has recently been shown to play important roles in several brain functions. A fraction of metabolized glucose molecules are being shunted through glycogen before reentering the glycolytic pathway, a phenomenon ...

journal_title：Neurochemical research

authors： Jakobsen E,Bak LK,Walls AB,Reuschlein AK,Schousboe A,Waagepetersen HS

更新日期：2017-09-01 00:00:00

abstract：:Hemin is a breakdown product of the blood protein, hemoglobin and is responsible for much of the secondary damage caused following a hemorrhagic stroke. Hemin is toxic to cultured astrocytes and it is thought that this toxicity is due to iron that is liberated when hemin is degraded. However, free iron applied to astr...

journal_title：Neurochemical research

authors： Owen JE,Bishop GM,Robinson SR

更新日期：2016-02-01 00:00:00

abstract：:Serotonin uptake and metabolism was studied in ependymal primary cultures. Serotonin uptake was facilitated by two different systems, one of which was the neuronal serotonin transporter SERT, exhibiting a Vmax value of 3.8+/-0.1 pmol x min(-1) x (mg protein)(-1) and an apparent Michaelis-Menten constant of 0.41+/-0.03...

journal_title：Neurochemical research

authors： Verleysdonk S,Hamprecht B,Rapp M,Wellard J

更新日期：2004-09-01 00:00:00

abstract：:Parkinson's disease (PD) is the second most prevalent age-related neurodegenerative disease with physiological manifestations including tremors, bradykinesia, abnormal postural reflexes, rigidity and akinesia and pathological landmarks showing losses of dopaminergic neurons in the substantia nigra. Although the etiolo...

journal_title：Neurochemical research

authors： Miller RL,James-Kracke M,Sun GY,Sun AY

更新日期：2009-01-01 00:00:00

abstract：:Previous studies have shown that hypoxia induces nitric oxide synthase-mediated generation of nitric oxide free radicals leading to peroxynitrite production. The present study tests the hypothesis that hypoxia results in NO-mediated modification of Na+, K+-ATPase in the fetal brain. Studies were conducted in guinea pi...

journal_title：Neurochemical research

authors： Qayyum I,Zubrow AB,Ashraf QM,Kubin J,Delivoria-Papadopoulos M,Mishra OP

更新日期：2001-10-01 00:00:00

abstract：:One hour incubation of rat cortical slices in a medium without oxygen and glucose (oxygen-glucose deprivation, OGD) increased S100B release to 6.53 ± 0.3 ng/ml/mg protein from its control value of 3.61 ± 0.2 ng/ml/mg protein. When these slices were then transferred to a medium containing oxygen and glucose (reoxygenat...

journal_title：Neurochemical research

authors： Demircan C,Gül Z,Büyükuysal RL

更新日期：2014-07-01 00:00:00

abstract：:The tumor suppressor PTEN (phosphatase and tensin homolog deleted on chromosome 10) is not only a protein, but also a lipid phosphatase that can negatively regulate the serine/threonine kinase Akt. It has been reported that PTEN can be regulated by means of phosphorylation. However, whether PTEN can be regulated by an...

journal_title：Neurochemical research

authors： Pei DS,Sun YF,Song YJ

更新日期：2009-08-01 00:00:00

abstract：:Thiamine deficiency (TD) produces severe neurodegenerative lesions. Studies have suggested that primary neurodegenerative events are associated with both oxidative stress and inflammation. Very little is known about the downstream effects on intracellular signaling pathways involved in neuronal death. The primary aim ...

journal_title：Neurochemical research

authors： Medeiros RCN,Moraes JO,Rodrigues SDC,Pereira LM,Aguiar HQDS,de Cordova CAS,Yim Júnior A,de Cordova FM

更新日期：2020-04-01 00:00:00

abstract：:We previously showed that extracellular levels of N-acetylaspartate (NAA) increase when a medium with reduced NaCl concentration is perfused through a microdialysis probe, and proposed that NAA may be released during hypoosmotic swelling. Here, we demonstrate that this effect is due to hypoosmolarity of the perfusion ...

journal_title：Neurochemical research

authors： Davies SE,Gotoh M,Richards DA,Obrenovitch TP

更新日期：1998-08-01 00:00:00

abstract：:The finding that ketamine, an anaesthetic, can elicit a rapid antidepressant effect at low doses that lasts for weeks in patients with depression is arguably a major achievement in psychiatry in the last decades. However, the mechanisms of action are unclear. The glutamatergic hypothesis of ketamine action posits that...

journal_title：Neurochemical research

authors： Stenovec M,Božić M,Pirnat S,Zorec R

更新日期：2020-01-01 00:00:00

abstract：:Glioblastoma is the most common and malignant brain cancer. In spite of surgical removal, radiation and chemotherapy, this cancer recurs within short time and median survival after diagnosis is less than a year. Glioblastoma stem cells (GSCs) left in the brain after surgery is thought to explain the inevitable recurre...

journal_title：Neurochemical research

authors： Skjellegrind HK,Fayzullin A,Johnsen EO,Eide L,Langmoen IA,Moe MC,Vik-Mo EO

更新日期：2016-07-01 00:00:00

abstract：:Myelinated axons were isolated by flotation from bovine pons, middle cerebellar peduncle, cervical spinal cord and three regions of the subcortical white matter. The myelinated axons were osmotically and mechanically shocked, followed by fractionation on a linear 15% sucrose to 45% sucrose density gradient. Axolemma-e...

journal_title：Neurochemical research

authors： Detskey PZ,Bigbee JW,DeVries GH

更新日期：1988-05-01 00:00:00

abstract：:The aggravated risk on intracerebral hemorrhage (ICH) with drugs used for stroke patients should be estimated carefully. We therefore established sensitive quantification methods and provided a rat ICH model for detection of ICH deterioration. In ICH intrastriatally induced by 0.014-unit, 0.070-unit, and 0.350-unit co...

journal_title：Neurochemical research

authors： Terai K,Suzuki M,Sasamata M,Miyata K

更新日期：2003-05-01 00:00:00

abstract：:Methylphenidate is commonly used for the treatment of attention deficit/hyperactivity disorder. There are still few works regarding the effects of methylphenidate on brain energy metabolism. Thus, in the present study we evaluated the effect of chronic administration of methylphenidate on the activities of mitochondri...

journal_title：Neurochemical research

authors： Fagundes AO,Aguiar MR,Aguiar CS,Scaini G,Sachet MU,Bernhardt NM,Rezin GT,Valvassori SS,Quevedo J,Streck EL

更新日期：2010-11-01 00:00:00

abstract：:Activation of cholinergic muscarinic receptors results in an increased turnover of membrane inositol phospholipids. In rat cerebral cortex slices, carbachol- and acetylcholine-induced inositol phosphates ([3H]InsPs) accumulation is maximal in 7 day-old rats and lowest in adults, while the density of muscarinic binding...

journal_title：Neurochemical research

authors： Balduini W,Costa LG,Murphy SD

更新日期：1990-01-01 00:00:00

abstract：:It has been established that oligodendrocytes, the myelin forming cells, participate in iron homeostasis through the synthesis and secretion of transferrin. Here we investigated whether a correlation exists between myelination, the commonly studied function of oligodendrocytes, and that of transferrin synthesis and se...

journal_title：Neurochemical research

authors： Espinosa de los Monteros A,Kumar S,Zhao P,Huang CJ,Nazarian R,Pan T,Scully S,Chang R,de Vellis J

更新日期：1999-02-01 00:00:00

abstract：:Sandhoff disease is an incurable neurodegenerative disorder caused by mutations in the lysosomal hydrolase β-hexosaminidase. Deficiency in this enzyme leads to excessive accumulation of ganglioside GM2 and its asialo derivative, GA2, in brain and visceral tissues. Small molecule inhibitors of ceramide-specific glucosy...

journal_title：Neurochemical research

authors： Arthur JR,Wilson MW,Larsen SD,Rockwell HE,Shayman JA,Seyfried TN

更新日期：2013-04-01 00:00:00

abstract：:The vanilloid receptor 1(VR1) is a nonselective cation channel that is activated by pungent vanilloid compound, extracellular protons, or noxious heat. mRNA of VR1 and vanilloid receptor 1-like receptor (VRL1) were expressed in PC12 cells, and only VRI mRNA was detected in glioma and A10 cell lines. VRI protein was de...

journal_title：Neurochemical research

authors： Qiao S,Li W,Tsubouchi R,Murakami K,Yoshino M

更新日期：2004-04-01 00:00:00

abstract：:It has been reported that central dopaminergic system is implicated in the mechanism underlying general anesthesia. Whether dopamine (DA) neurons in midbrain ventral periaqueductal gray (vPAG) are involved in general anesthesia and how general anesthetics affect these neurons remain sparsely documented. To determine t...

journal_title：Neurochemical research

authors： Li J,Yu T,Shi F,Zhang Y,Duan Z,Fu B,Zhang Y

更新日期：2018-04-01 00:00:00

abstract：:Human dental follicle cells (DFCs) are ectomesenchymal multipotent stem cells that form spheroid cell clusters (SCCs) under serum free medium cell culture conditions (SFM). Until today, molecular mechanisms for the formation of SCCs are unknown. In this study a quantitative phosphoproteomics approach revealed regulate...

journal_title：Neurochemical research

authors： Beck HC,Gosau M,Kristensen LP,Morsczeck C

更新日期：2014-07-01 00:00:00

abstract：:Human neuroblastoma cholinergic LA-N-2 cells were used as an experimental model to test the possibility that the methylation of phosphoethanolamine (PEtn) to phosphocholine (PCho) and free choline (Cho) (Andriamampandry et al. 1989) could contribute to acetylcholine (AcCho) synthesis. LA-N-2 cells were incubated with ...

journal_title：Neurochemical research

authors： Haidar NE,Carrara M,Andriamampandry C,Kanfer JN,Freysz L,Dreyfus H,Massarelli R

更新日期：1994-01-01 00:00:00

abstract：:Metabolic modeling of dynamic (13)C labeling curves during infusion of (13)C-labeled substrates allows quantitative measurements of metabolic rates in vivo. However metabolic modeling studies performed in the brain to date have only modeled time courses of total isotopic enrichment at individual carbon positions (posi...

journal_title：Neurochemical research

authors： Shestov AA,Valette J,Deelchand DK,Uğurbil K,Henry PG

更新日期：2012-11-01 00:00:00

abstract：:Glucocorticoid receptors (GRs) exert actions on the hippocampus that are important for memory formation. There are correlations between vascular dysfunctions and GR-related gene expression. Both vascular dysfunction and GR gene expression decline occur during the ageing process. Therefore, hypotensors, which have effe...

journal_title：Neurochemical research

authors： Li X,Ma J,Xu J,Zhu D,Li A,Che Y,Chen D,Feng X

更新日期：2017-11-01 00:00:00

abstract：:Implication of protein phosphatases in Alzheimer disease led us to a systemic investigation of the identification of these enzyme activities in human brain. Human brain phosphatases eluted from DEAE-Sephacel with 0.22 M NaCl were resolved into two main groups by affi-gel blue chromatography, namely affi-gel blue-bindi...

journal_title：Neurochemical research

authors： Cheng LY,Wang JZ,Gong CX,Pei JJ,Zaidi T,Grundke-Iqbal I,Iqbal K

更新日期：2000-01-01 00:00:00

abstract：:Propofol and ketamine may provide certain degree of neuroprotection, but the underlying mechanism remains unclear to date. The cAMP response element-binding protein (CREB) was proposed that its phosphorylation at Ser133 (P-CREB) constituted a convergence point involved in neuroprotection. The purpose of this study was...

journal_title：Neurochemical research

authors： Shu L,Li T,Han S,Ji F,Pan C,Zhang B,Li J

更新日期：2012-01-01 00:00:00