Abstract:
:Serotonin uptake and metabolism was studied in ependymal primary cultures. Serotonin uptake was facilitated by two different systems, one of which was the neuronal serotonin transporter SERT, exhibiting a Vmax value of 3.8+/-0.1 pmol x min(-1) x (mg protein)(-1) and an apparent Michaelis-Menten constant of 0.41+/-0.03 microM. The main product of metabolism was 5-hydroxyindole acetic acid, which resulted from the action of monoamine oxidase A. This enzyme showed a maximal rate of 0.85+/-0.02 nmol x min(-1) x (mg protein)(-1) and a Michaelis-Menten constant of 78+/-5 microM. Ependymal cells were able to dispose of extracellular serotonin with initial rates of approximately 600 pmol x min(-1) x (mg protein)(-1) and of 4 pmol x min(-1) x (mg protein)(-1) when challenged with 500 microM and 1 microM extracellular serotonin, respectively. Ependymal cells are concluded to facilitate the "sink" action of the CSF by removing waste compounds upon passing of the fluid from the parenchymal extracellular space into the ventricular system.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Verleysdonk S,Hamprecht B,Rapp M,Wellard Jdoi
10.1023/b:nere.0000035810.08543.97keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
1739-47issue
9eissn
0364-3190issn
1573-6903journal_volume
29pub_type
杂志文章abstract::CNTF (ciliary neurotrophic factor) has been suggested to be an important survival factor for oligodendrocytes; however, this effect is inconsistently obtained and myelination appears normal in CNTF null animals. On the other hand, CNTF stimulates astrocytes to produce growth and trophic factors. Therefore, we tested t...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9151-6
更新日期:2007-02-01 00:00:00
abstract::Tumor Necrosis Factor Receptor Super Family 6 gene (TNFRSF6), also known as FAS, encodes the Fas antigen, a cell surface receptor mediating cell apoptosis, situated on chromosome 10q located near the region of linkage to sporadic Alzheimer's disease (sAD). FAS levels have been reported elevated in the brain of AD pati...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9329-6
更新日期:2007-09-01 00:00:00
abstract::In a study of the role of taurine in the genesis of epilepsy the effects of metrazol-induced convulsions on the uptake and distribution of taurine in the brain were measured. In vivo we found no significant uptake of taurine in the mouse brain; in rabbit brain in most areas significant taurine uptake was found. The ph...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964640
更新日期:1979-08-01 00:00:00
abstract::The concentration of vitamin E (alpha-tocopherol) was measured in samples of cortex from patients with Alzheimer's disease (AD), fetuses with Down's syndrome (DS), and also in a group of centenarians. The mean tocopherol concentrations in the two patient groups did not differ significantly from appropriate controls. W...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965511
更新日期:1989-12-01 00:00:00
abstract::Convection enhanced delivery of 6-hydroxydopamine (6-OHDA) to the rat striatum results in a model of Parkinson's disease. An important feature of this unilateral model is the progressive loss of dopaminergic (DA) neurons over the course of several weeks. To improve the understanding of this model, gene expression chan...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0133-3
更新日期:2010-05-01 00:00:00
abstract::CNS trauma has been associated with an increase in free radical production, but the cellular sources of this increase or the mechanism involved in the production of free radicals are not known. We, therefore, investigated the effects of trauma on free radical production in cultured neurons, astrocytes and BV-2 microgl...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1014875210852
更新日期:2002-02-01 00:00:00
abstract::The abnormal production of short chain fatty acid (SCFAs) caused by gut microbial dysbiosis plays an important role in the pathogenesis and progression of Parkinson's disease (PD). This study sought to evaluate how butyrate, one of SCFAs, affect the pathology in a subacute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03074-3
更新日期:2020-09-01 00:00:00
abstract::We evaluated oxidative stress associated with a model of experimental epilepsy. Male Wistar rats were injected i.p. with 150 mg/kg convulsant 3-mercaptopropionic acid and decapitated in two stages: during seizures or in the post-seizure period. Spontaneous chemiluminescence, levels of thiobarbituric acid reactive subs...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF03177490
更新日期:1998-12-01 00:00:00
abstract::Estrogen action is necessary for evidencing the stimulatory action of mifepristone and naloxone on prolactin (PRL) secretion during late pregnancy. Our aim is to determine the mechanism mediating this facilitator action of estrogens. To investigate the hypothalamic mechanisms involved in estrogen actions in PRL secret...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2067-x
更新日期:2016-12-01 00:00:00
abstract::We have developed a coupled Xenopus oocyte expression system for evaluating the functional effects of mutations in known or suspected adhesion molecules, which allows for a very rapid assessment of intercellular adhesion. As a model protein, we first used Protein zero (Po), an adhesion molecule that mediates self-adhe...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1010999622760
更新日期:2001-06-01 00:00:00
abstract::The study provides detailed biochemical correlates to the common histopathological diagnoses in epilepsy. A dot immunobinding procedure was used for quantification of NSE, GFA, S-100, NCAM, NF 68 and NF 200. The material consisted of samples from 48 patients either selected for surgical treatment of partial epilepsy o...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967255
更新日期:1993-04-01 00:00:00
abstract::The effect of 4-aminopyridine on [3H]acetylcholine release was studied in rat cerebral cortical synaptosomes in the presence of a several secretagogues that have different mechanisms of action. As found previously, 4-aminopyridine increased [3H]acetylcholine release in a concentration-dependent manner (5-10 mM); a hig...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969632
更新日期:1989-02-01 00:00:00
abstract::We measured the activity of several acid hydrolases in oligodendrocyte and mixed glial (predominantly astrocytic) cell cultures prepared from neonatal rat cerebra. When compared with the mixed glial cultures, the cultured oligodendrocytes exhibited higher levels for all the hydrolases when activities were normalized t...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970764
更新日期:1988-10-01 00:00:00
abstract::Members of the PDGF family have multiple roles during embryogenesis and in a variety of pathological situations in the adult. One of the major sites of PDGF-B expression in adult mammals are postmitotic CNS neurons. Combined with reported neurotrophic and neuroprotective effects of exogenously administered PDGFs, this...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022421001288
更新日期:2003-02-01 00:00:00
abstract::Glutamine synthetase (GS) is the key enzyme in cerebral glutamine production. Understanding the regulation of the expression of GS is important for definition of the control of glutamine metabolism in brain. Therefore, we studied the control of GS expression by 8-bromo cyclic AMP in primary cultures of astrocytes prep...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973746
更新日期:1990-06-01 00:00:00
abstract::The Fatty Acid method was used to determine whether incorporation of plasma radiolabeled arachidonic acid into brain phospholipids is controlled by phospholipase A2. Awake rats received an i.v. injection of a phospholipase A2 inhibitor, manoalide (10 mg/kg), and then were infused i.v. with [1-(14)C]arachidonate or [3H...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020788031720
更新日期:1998-10-01 00:00:00
abstract::Neurovascular injury comprises a wide spectrum of pathophysiology that underlies the progression of brain injury after cerebral ischemia. Recently, it has been shown that activation of the integrin-associated protein CD47 mediates the development of blood-brain barrier injury and edema after cerebral ischemia. However...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0159-6
更新日期:2010-07-01 00:00:00
abstract::Any spinal cord injury carries the potential for persistent disability affecting motor, sensory and autonomic functions. To prevent this outcome, it is highly desirable to block a chain of deleterious reactions developing in the spinal areas immediately around the primary lesion. Thus, early timing of pharmacological ...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-017-2459-6
更新日期:2019-01-01 00:00:00
abstract::DYT1 dystonia is caused by a glutamic acid deletion (ΔE) in the endoplasmic reticulum (ER) protein torsinA. Previous studies suggest that torsinA modulates the aggregation of cytosolic misfolded proteins and ER stress responses, although the mechanisms underlying those effects remain unclear. In order to investigate t...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0363-4
更新日期:2011-03-01 00:00:00
abstract::Pathogens such as bacterial lipopolysaccharide (LPS) play an important role in promoting the production of the inflammatory cytokines interleukin-1 beta (IL-1β) and tumour necrosis factor-α (TNF-α) in response to infection or damage in microglia. However, whether different signalling pathways regulate these two inflam...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-019-02895-1
更新日期:2019-12-01 00:00:00
abstract::Parkinson's disease (PD) is a neurodegenerative disease, with genetics and environment contributing to the disease onset. The limited pathological cognize of the disease restrained the approaches to improve the clinical treatment. Recently, studies showed that endoplasmic reticulum (ER) stress played an important role...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1922-0
更新日期:2016-08-01 00:00:00
abstract::Status epilepticus is a common manifestation of nerve agent toxicity and represents a serious medical emergency with high rates of mortality and neurologic injury in those that survive. The aim of the current study was to determine if targeting oxidative stress with the catalytic antioxidant, AEOL10150, would reduce p...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2273-1
更新日期:2017-07-01 00:00:00
abstract::In membranes of rat striatum, phorbol 12-myristate 13-acetate (PMA), a potent activator of Ca2+/phospholipid-dependent protein kinase, enhanced adenylate cyclase activity by counteracting the inhibition elicited by GTP. Exposure to pertussis toxin caused a similar alteration of the GTP-regulation of the enzyme activit...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01101712
更新日期:1990-11-01 00:00:00
abstract::The experiments reported in this paper address the hypothesis that [3H]leucine enkephalin labels both mu and delta receptors. As reported by other workers, this peptide dissociates from rat brain membranes in a biphasic manner. This is consistent with a two site binding model which hypothesizes that the peptide labels...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963918
更新日期:1983-02-01 00:00:00
abstract::Regional and developmental changes in metabolite concentrations were measured by 1H NMR spectroscopy and HPLC of perchloric acid extracts from rat brain and eye. The highest concentrations of N-acetylaspartate were found in grey matter as opposed to white matter with concentration increasing with age from neonate to a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02532417
更新日期:1996-09-01 00:00:00
abstract::Brain development is a highly orchestrated complex process. The developing brain utilizes many substrates including glucose, ketone bodies, lactate, fatty acids and amino acids for energy, cell division and the biosynthesis of nucleotides, proteins and lipids. Metabolism is crucial to provide energy for all cellular p...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-015-1600-7
更新日期:2015-12-01 00:00:00
abstract::Rabbits sensitized with whole nervous tissue or myelin basic protein (MBP) plus adjuvant and developing experimental allergic encephalomyelitis (EAE) were studied for the presence of oligoclonal immunoglobulin (Ig) bands in spinal fluid and serum. Samples obtained prior to sensitization and at the time of sacrifice we...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963909
更新日期:1981-01-01 00:00:00
abstract::Sonic hedgehog (Shh), a secreted glycoprotein factor, can activate the Shh pathway, which has been implicated in neuronal polarization involving neurite outgrowth. However, little evidence is available about the effect of Shh on neurite outgrowth in primary cortical neurons and its potential mechanism. Here, we reveal...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1736-5
更新日期:2016-04-01 00:00:00
abstract::Mice with a heterozygous knock-out of the myelin protein P0 gene (P0+/-) develop a neuropathy similar to human Charcot-Marie-Tooth disease. They are indistinguishable from wild-types (WT) at birth and develop a slowly progressing demyelinating neuropathy. The aim of this study was to investigate whether the regenerati...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1030-3
更新日期:2013-06-01 00:00:00
abstract::Hyperhomocysteinemia occurs in homocystinuria, an inherited metabolic disease clinically characterized by thromboembolic episodes and a variable degree of neurological dysfunction whose pathophysiology is poorly known. In this study, we induced elevated levels of homocysteine (Hcy) in blood (500 microM), comparable to...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1021670607647
更新日期:2002-12-01 00:00:00