Uptake and metabolism of serotonin by ependymal primary cultures.

Abstract:

:Serotonin uptake and metabolism was studied in ependymal primary cultures. Serotonin uptake was facilitated by two different systems, one of which was the neuronal serotonin transporter SERT, exhibiting a Vmax value of 3.8+/-0.1 pmol x min(-1) x (mg protein)(-1) and an apparent Michaelis-Menten constant of 0.41+/-0.03 microM. The main product of metabolism was 5-hydroxyindole acetic acid, which resulted from the action of monoamine oxidase A. This enzyme showed a maximal rate of 0.85+/-0.02 nmol x min(-1) x (mg protein)(-1) and a Michaelis-Menten constant of 78+/-5 microM. Ependymal cells were able to dispose of extracellular serotonin with initial rates of approximately 600 pmol x min(-1) x (mg protein)(-1) and of 4 pmol x min(-1) x (mg protein)(-1) when challenged with 500 microM and 1 microM extracellular serotonin, respectively. Ependymal cells are concluded to facilitate the "sink" action of the CSF by removing waste compounds upon passing of the fluid from the parenchymal extracellular space into the ventricular system.

journal_name

Neurochem Res

journal_title

Neurochemical research

authors

Verleysdonk S,Hamprecht B,Rapp M,Wellard J

doi

10.1023/b:nere.0000035810.08543.97

keywords:

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

1739-47

issue

9

eissn

0364-3190

issn

1573-6903

journal_volume

29

pub_type

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