Uptake and metabolism of serotonin by ependymal primary cultures.


:Serotonin uptake and metabolism was studied in ependymal primary cultures. Serotonin uptake was facilitated by two different systems, one of which was the neuronal serotonin transporter SERT, exhibiting a Vmax value of 3.8+/-0.1 pmol x min(-1) x (mg protein)(-1) and an apparent Michaelis-Menten constant of 0.41+/-0.03 microM. The main product of metabolism was 5-hydroxyindole acetic acid, which resulted from the action of monoamine oxidase A. This enzyme showed a maximal rate of 0.85+/-0.02 nmol x min(-1) x (mg protein)(-1) and a Michaelis-Menten constant of 78+/-5 microM. Ependymal cells were able to dispose of extracellular serotonin with initial rates of approximately 600 pmol x min(-1) x (mg protein)(-1) and of 4 pmol x min(-1) x (mg protein)(-1) when challenged with 500 microM and 1 microM extracellular serotonin, respectively. Ependymal cells are concluded to facilitate the "sink" action of the CSF by removing waste compounds upon passing of the fluid from the parenchymal extracellular space into the ventricular system.


Neurochem Res


Neurochemical research


Verleysdonk S,Hamprecht B,Rapp M,Wellard J





Has Abstract


2004-09-01 00:00:00












  • Selective damage in striatum and hippocampus with in vitro anoxia.

    abstract::An in vitro model of anoxia-induced brain damage was utilized to help elucidate the biochemical basis of cell damage due to reduced oxygen availability. Previous studies suggest that anoxia-induced damage may vary presynaptically, post-synaptically or in the cell body. Thus, the consequences of an anoxic treatment inc...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Gibson GE,Freeman GB,Mykytyn V

    更新日期:1988-04-01 00:00:00

  • Three TRH-like molecules are released from rat hypothalamus in vitro.

    abstract::TRH-like immunoreactivity distinct from TRH is present in various tissues and fluids. In order to determine whether TRH-like molecules are secreted by the hypothalamus, we analyzed tissues and media from hypothalamic slices incubated in Krebs Ringer bicarbonate. Media from basal or high KCl conditions contained 3 TRH-...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Méndez M,Cisneros M,Baez A,Joseph-Bravo P,Charli JL

    更新日期:1999-07-01 00:00:00

  • Oxygen glucose deprivation (OGD)/re-oxygenation-induced in vitro neuronal cell death involves mitochondrial cyclophilin-D/P53 signaling axis.

    abstract::Oxidative stress-induced neuronal cell death requires opening of the mitochondrial permeability transition pore. P53 mitochondrial translocation and association with Cyclophilin D (Cyp-D) is required for the pore opening. Here we tested this signaling axis in oxygen glucose deprivation (OGD)/re-oxygenation-induced in ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Zhao LP,Ji C,Lu PH,Li C,Xu B,Gao H

    更新日期:2013-04-01 00:00:00

  • Voltage Gated Potassium Channel Kv1.3 Is Upregulated on Activated Astrocytes in Experimental Autoimmune Encephalomyelitis.

    abstract::Kv1.3 is a voltage gated potassium channel that has been implicated in pathophysiology of multiple sclerosis (MS). In the present study we investigated temporal and cellular expression pattern of this channel in the lumbar part of spinal cords of animals with experimental autoimmune encephalomyelitis (EAE), animal mod...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Bozic I,Tesovic K,Laketa D,Adzic M,Jakovljevic M,Bjelobaba I,Savic D,Nedeljkovic N,Pekovic S,Lavrnja I

    更新日期:2018-05-01 00:00:00

  • Possible occurrence of ornithine-omega-aminotransferase in GABAergic neurons.

    abstract::The specific precursors for neurotransmitter pools of glutamate giving rise to GABA in GABAergic neurons and nerve endings have not been clearly established. Glutamate is the immediate precursor for the production of GABA and it is suggested that ornithine (from arginine) might be serving as one of the precursors of g...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Sadasivudu B,Swamy M

    更新日期:1984-11-01 00:00:00

  • Treadmill Exercise Promotes Neurogenesis in Ischemic Rat Brains via Caveolin-1/VEGF Signaling Pathways.

    abstract::Using a model of middle cerebral artery occlusion (MCAO), we have previously demonstrated that treadmill exercise promotes angiogenesis in the ischemic penumbra through caveolin-1/VEGF signaling pathways. However, the function of caveolin-1/VEGF signaling in neurogenesis after MCAO has not been determined. In this stu...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Zhao Y,Pang Q,Liu M,Pan J,Xiang B,Huang T,Tu F,Liu C,Chen X

    更新日期:2017-02-01 00:00:00

  • Protein analysis of myelin isolated from the CNS of fish: developmental and species comparisons.

    abstract::The protein composition of myelin isolated from the CNS of four different fish species (trout, goldfish), eel, Tilapia) was analysed by SDS-polyacrylamide gel electrophoresis and compared with that of pig and rat brain. Thereby the following features were found typical to the myelin of fish: (1) a basic protein of par...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Jeserich G

    更新日期:1983-08-01 00:00:00

  • MEKK1 Associated with Neuronal Apoptosis Following Intracerebral Hemorrhage.

    abstract::The JNKs have been implicated in a variety of biological functions in mammalian cells, including apoptosis and the responses to stress. However, the physiological role of these pathways in the intracerebral hemorrhage (ICH) has not been fully elucidated. In this study, we identified a MAPK kinase kinase (MAPKKK), MEKK...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Lu H,Ning X,Tao X,Ren J,Song X,Tao W,Zhu L,Han L,Tao T,Yang J

    更新日期:2016-12-01 00:00:00

  • The protective effect of N-acetylcysteine on oxidative stress in the brain caused by the long-term intake of aspartame by rats.

    abstract::Long-term intake of aspartame at the acceptable daily dose causes oxidative stress in rodent brain mainly due to the dysregulation of glutathione (GSH) homeostasis. N-Acetylcysteine provides the cysteine that is required for the production of GSH, being effective in treating disorders associated with oxidative stress....

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Finamor IA,Ourique GM,Pês TS,Saccol EM,Bressan CA,Scheid T,Baldisserotto B,Llesuy SF,Partata WA,Pavanato MA

    更新日期:2014-09-01 00:00:00

  • The effects of transmitters on the affinity of the insoluble fraction of ox brain for Na+ and K+.

    abstract::The insoluble fraction of ox-brain, which had previously been shown to have a non-linear affinity for Na+ and K+, was prepared. Acetylcholine (1 x 10(-8) mol/l and 1 x 10(-7) mol/l) reduced the affinity of the fraction for Na+ and K+ to zero, while at 1 x 10(-6) mol/l, the affinity for the cations was almost as high a...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Ahmed N,Hillman H

    更新日期:1989-02-01 00:00:00

  • Functional analysis for peripheral myelin protein PASII/PMP22: is it a member of claudin superfamily?

    abstract::Two major glycoproteins, P0 and PASII/PMP22, are specifically expressed in peripheral myelin. Point mutations of these proteins and over or under expression of PASII/PMP22 cause various hereditary peripheral neuropathies. P0 is well characterized as a major adhesion molecule in PNS myelin, but the function of PASII/PM...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Takeda Y,Notsu T,Kitamura K,Uyemura K

    更新日期:2001-06-01 00:00:00

  • NPD1 induction of retinal pigment epithelial cell survival involves PI3K/Akt phosphorylation signaling.

    abstract::Neuroprotectin D1 (NPD1), a docosahexaenoic acid (DHA)-derived lipid mediator, promotes survival in cells exposed to oxidative stress by inducing the activity of anti-inflammatory mediators and suppressing the expression of pro-inflammatory genes. Though retinal pigment epithelial (RPE) cells naturally produce NPD1 fr...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Halapin NA,Bazan NG

    更新日期:2010-12-01 00:00:00

  • Microarray analysis of gene expression in rat hippocampus after chronic ethanol treatment.

    abstract::It is thought that changes in gene expression in the brain mediate chronic ethanol-induced complex behaviors such as tolerance, dependence, and sensitization, and also relate to ethanol-induced brain toxicity. Using high-density filter-based cDNA microarrays (GeneFilters), we analyzed the expression of over 5000 genes...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Saito M,Smiley J,Toth R,Vadasz C

    更新日期:2002-10-01 00:00:00

  • Prevention of monensin-induced hyperpolarization in NG108-15 cells.

    abstract::The NG 108-15 (neuroblastoma X glioma hybrid) cell line was used as an in vitro neuronal model to evaluate potential antagonists of the Na+-selective carboxylic ionophore monensin. Changes in membrane electrical characteristics induced by monensin with and without the simultaneous administration of antagonists were me...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Doebler JA

    更新日期:2000-07-01 00:00:00

  • Harpagophytum procumbens prevents oxidative stress and loss of cell viability in vitro.

    abstract::Harpagophytum procumbens, popularly known as devil's claw, is a plant commonly used in the treatment of diseases of inflammatory origin. The anti-inflammatory effects of H. procumbens have been studied; however, the mechanism of action is not elucidated. It is known that excess of reactive oxygen and nitrogen species ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Schaffer LF,Peroza LR,Boligon AA,Athayde ML,Alves SH,Fachinetto R,Wagner C

    更新日期:2013-11-01 00:00:00

  • Role of selenium on calcium signaling and oxidative stress-induced molecular pathways in epilepsy.

    abstract::Epilepsy is one of the oldest neurological conditions known to humankind. It is known that oxidative stress and generation of reactive oxygen species are a cause and consequence of epileptic seizures. Although recent years have seen tremendous progress in the molecular biology and metabolism of selenium, we still know...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Nazıroglu M

    更新日期:2009-12-01 00:00:00

  • Effect of Imipramine, Paroxetine, and Lithium Carbonate on Neurobehavioral Changes of Streptozotocin in Rats: Impact on Glycogen Synthase Kinase-3 and Blood Glucose Level.

    abstract::Recent studies have demonstrated a scrutinized association of diabetes mellitus with depressive symptoms and major depression. Glycogen synthase kinase-3 (GSK-3) is a protein kinase enzyme constitutively active in non-stimulated cells and in multiple signalings. Independent lines of research provide a converging evide...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Nadeem RI,Ahmed HI,El-Denshary EE

    更新日期:2015-09-01 00:00:00

  • All Trans Retinoic Acid Attenuates Markers of Neuroinflammation in Rat Brain by Modulation of SIRT1 and NFκB.

    abstract::Alcohol abuse affects several neurological pathways and causes significant alterations in the brain. Abstention from alcohol causes only a marginal decrease in oxidative stress and neuro inflammation. Our previous studies had shown that an active metabolite of vitamin A, all trans retinoic acid (ATRA), ameliorates alc...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Priyanka SH,Syam Das S,Thushara AJ,Rauf AA,Indira M

    更新日期:2018-09-01 00:00:00

  • Inhibition of brain energy metabolism by the branched-chain amino acids accumulating in maple syrup urine disease.

    abstract::In the present work we investigated the in vitro effect of the branched-chain amino acids (BCAA) accumulating in maple syrup urine disease (MSUD) on some parameters of energy metabolism in cerebral cortex of rats. 14CO2 production from [1-14C]acetate, [1-5-14C]citrate and [U-14C]glucose, as well as glucose uptake by t...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Ribeiro CA,Sgaravatti AM,Rosa RB,Schuck PF,Grando V,Schmidt AL,Ferreira GC,Perry ML,Dutra-Filho CS,Wajner M

    更新日期:2008-01-01 00:00:00

  • Glial expression of interleukin-18 and its receptor after excitotoxic damage in the mouse hippocampus.

    abstract::Interleukin (IL)-18, a member of the IL-1 cytokine family, is an important mediator of peripheral inflammation and host defence responses. However, although IL-1 is a key proinflammatory cytokine in the brain, little is known about IL-18 changes in glial cells under excitotoxic neurodegeneration. In this study, we cha...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Jeon GS,Park SK,Park SW,Kim DW,Chung CK,Cho SS

    更新日期:2008-01-01 00:00:00

  • N-acetylaspartic acid (NAA) and N-acetylaspartylglutamic acid (NAAG) in human ventricular, subarachnoid, and lumbar cerebrospinal fluid.

    abstract::N-Acetylaspartic and N-acetylaspartylglutamic acid concentrations in human ventricular, subarachnoid and lumbar cerebrospinal fluid were measured by combined gas chromatography-mass spectrometry using selected ion monitoring with deuterated internal standards. N-Acetylaspartate concentrations were in the range 55, 9, ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Faull KF,Rafie R,Pascoe N,Marsh L,Pfefferbaum A

    更新日期:1999-10-01 00:00:00

  • Isolation of human nerve growth factor from placental tissue.

    abstract::Nerve growth factor (NGF) has been isolated from human placental tissue. Using the chicken embryo dorsal root ganglia assay, we determined levels of NGF activity for the amnion, placental cotyledons, cord serum, fetal serum, and maternal serum. The highest levels of NGF activity were measured in placental cotyledons. ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Goldstein LD,Reynolds CP,Perez-Polo JR

    更新日期:1978-04-01 00:00:00

  • Metabonomic characterization of the 3-nitropropionic acid rat model of Huntington's disease.

    abstract::3-Nitropropionic acid (3-NP)-induced neurotoxicity can be used as a model for the genetic neurodegenerative disorder Huntington's disease (HD). A metabolic profiling strategy was adopted to explore the biochemical consequences of 3-NP administered to rats in specific brain regions. (1)H NMR spectroscopy was used to ch...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Tsang TM,Haselden JN,Holmes E

    更新日期:2009-07-01 00:00:00

  • Partial purification of a phosphoethanolamine methyltransferase from rat brain cytosol.

    abstract::The conversion of phosphoethanolamine to phosphocholine requires 3 separate N-methyltransferases. We had previously purified the enzyme catalyzing the last methylation, phosphodimethylethanolamine N-methyltransferase. We have successfully purified the enzyme catalyzing the initial methylation of phosphoethanolamine. A...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Mukherjee S,Freysz L,Kanfer JN

    更新日期:1995-10-01 00:00:00

  • Cholinergic systems in muscle and brain in vitamin E-deficient rats.

    abstract::Rats fed a vitamin E-deficient diet for 7--8 weeks postweaning showed no change in brain weight or the activity in brain of various enzymes involved in neurotransmitter synthesis and metabolism. Body and muscle weights were markedly reduced. Muscle choline acetyltransferase and acetylcholinesterase activities were sig...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Sung SC,Sandberg PR,McGeer EG

    更新日期:1978-12-01 00:00:00

  • The relationship between taurine and 3-nitrotyrosine level of hepatocytes in experimental endotoxemia.

    abstract::It has been proposed that taurine may function as an oxidant in a dose-dependent manner in vivo and in vitro. The present study was carried out to investigate the relationship between taurine concentration and 3-nitrotyrosine level, a stable marker of peroxynitrite action, in hepatocytes of guinea pig in endotoxemia b...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Erdamar H,Türközkan N,Balabanli B,Ozan G,Bircan FS

    更新日期:2007-11-01 00:00:00

  • Hypoosmolarity induces an increase of extracellular N-acetylaspartate concentration in the rat striatum.

    abstract::We previously showed that extracellular levels of N-acetylaspartate (NAA) increase when a medium with reduced NaCl concentration is perfused through a microdialysis probe, and proposed that NAA may be released during hypoosmotic swelling. Here, we demonstrate that this effect is due to hypoosmolarity of the perfusion ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Davies SE,Gotoh M,Richards DA,Obrenovitch TP

    更新日期:1998-08-01 00:00:00

  • Effects of L-carnitine on the formation of fatty acid ethyl esters in brain and peripheral organs after short-term ethanol administration in rat.

    abstract::A study was undertaken in rats to evaluate the effects of short-term oral ethanol administration on the levels of fatty acid ethyl esters (FAEE) in brain and peripheral organs in the presence and absence of pretreatment with L-carnitine. Administration of ethanol to rats for seven days resulted in fatty acid ethyl est...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Calabrese V,Rizza V

    更新日期:1999-01-01 00:00:00

  • Carbachol-induced hydrolysis of phospholipids in hippocampal slices may be mediated in part by subsequent activation of metabotropic glutamate receptors.

    abstract::We observed that AP-3, an antagonist of metabotropic glutamate receptors, reduced carbachol-induced hydrolysis of phospholipids in hippocampal slices. This inhibition could be explained in different ways, e.g.: 1) AP-3 acts also as antagonist of muscarinic receptors mediating the hydrolysis of phospholipids induced by...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Llansola M,Hermenegildo C,Miñana MD,Felipo V

    更新日期:1998-06-01 00:00:00

  • Apoptotic mode of cell death in substantia nigra following intranigral infusion of the parkinsonian neurotoxin, MPP+ in Sprague-Dawley rats: cellular, molecular and ultrastructural evidences.

    abstract::The potent parkinsonian neurotoxin 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) is known to cause dopaminergic neurodegeneration in nigrostriatal system. In the present study we investigated the nuclear morphology of cells in the substantia nigra pars compacta (SNpc) region of rats following unilateral intra...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Banerjee R,Sreetama S,Saravanan KS,Dey SN,Mohanakumar KP

    更新日期:2007-07-01 00:00:00