Abstract：
:Protocatechuic acid (PA), a major metabolite of anthocyanins, has been reported to possess antioxidant and anti-inflammatory activities. However, the effects of PA on LPS-induced inflammatory responses in microglia have not been reported. The aim of this study was to investigate the anti-inflammatory effects and molecular mechanisms of PA on LPS-stimulated BV2 microglia. The production of inflammatory mediators TNF-α, IL-6, IL-1β, and PGE2 were detected by ELISA. TLR4, NF-κB and MAPKs activation were detected by western blotting. Our results demonstrated that PA dose-dependently inhibited LPS-induced TNF-α, IL-6, IL-1β, and PGE2 production. In addition, PA suppressed LPS-induced TLR4 expression, NF-κB and MAPKs activation, which resulted in the inhibition of inflammatory mediators. In conclusion, these results suggested that PA exhibited anti-inflammatory effects on LPS-stimulated BV2 microglia and the mechanisms were involved in the inhibition of TLR4-mediated NF-κB and MAPKs signaling pathways.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Wang HY,Wang H,Wang JH,Wang Q,Ma QF,Chen YYdoi
10.1007/s11064-015-1646-6subject
Has Abstractpub_date
2015-08-01 00:00:00pages
1655-60issue
8eissn
0364-3190issn
1573-6903journal_volume
40pub_type
杂志文章abstract：:In vitro synthesis of myelin proteolipid protein (PLP) was explored at different ages using rat brain total homogenates, incubated for 30 min with [3H]glycine. Total proteolipids, extracted from the incubated samples, were separated by SDS-PAGE and the radioactivity was measured in the band corresponding to myelin PLP...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973044
更新日期：1984-09-01 00:00:00
abstract：:The SAM strains, a group of related inbred strains consisting of senescence-prone inbred strains (SAMP) and senescence-resistant inbred strains (SAMR), have been successfully developed by selective inbreeding of the AKR/J strain of mice donated by the Jackson laboratory in 1968. The characteristic feature of aging com...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-009-9922-y
更新日期：2009-04-01 00:00:00
abstract：:Oxidative stress-induced neuronal cell death requires opening of the mitochondrial permeability transition pore. P53 mitochondrial translocation and association with Cyclophilin D (Cyp-D) is required for the pore opening. Here we tested this signaling axis in oxygen glucose deprivation (OGD)/re-oxygenation-induced in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-0968-5
更新日期：2013-04-01 00:00:00
abstract：:Nerve growth factor (NGF) has been isolated from human placental tissue. Using the chicken embryo dorsal root ganglia assay, we determined levels of NGF activity for the amnion, placental cotyledons, cord serum, fetal serum, and maternal serum. The highest levels of NGF activity were measured in placental cotyledons. ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964058
更新日期：1978-04-01 00:00:00
abstract：:Using the replication-defective adenovirus vector, we overexpressed rat glia maturation factor (GMF) in primary astrocyte cultures derived from embryonic rat brains. Among the three isoforms of MAP kinase, there was a big increase in the phosphorylation of p38, as detected with Western blotting using the phosphospecif...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1014241300179
更新日期：2001-12-01 00:00:00
abstract：:Alterations in lysosomal proteases have been implicated in many neurodegenerative diseases. The current study demonstrates a concentration-dependent decrease in PC12 cell viability and transient changes in cystatin C (CYSC), cathepsin B (CATB), cathepsin D (CATD) and caspase-3 following exposure to H2O2. Furthermore, ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9338-5
更新日期：2007-09-01 00:00:00
abstract：:A cortical cup model with continuous perfusion of artificial cerebrospinal fluid (containing 134 mM NaCl) was used to investigate the effects of anion channel blockers on the hyposmotically-induced release of amino acids from the in vivo rat cerebral cortex. The hyposmotic stimulus (25 mM NaCl) evoked a release of tau...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020902104056
更新日期：1999-03-01 00:00:00
abstract：:We investigated the action of the new hypothalamic proline-rich peptide (PRP-1), normally produced by neurosecretory cells of hypothalamic nuclei (NPV and NSO), 3 and 4 weeks following rat sciatic nerve transection. The impulse activity flow of interneurons (IN) and motoneurons (MN) on stimulation of mixed (n. ischiad...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-2685-1
更新日期：2005-04-01 00:00:00
abstract：:Depression afflicts more than 300 million people worldwide, but there is currently no universally effective drug in clinical practice. In this study, chronic restraint stress (CRS)-induced mice depression model was used to study the antidepressant effects of resveratrol and its mechanism. Our results showed that resve...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03200-1
更新日期：2021-01-03 00:00:00
abstract：:Beta-amyloid (Abeta) peptides may cause malfunction and death of neurons in Alzheimer's disease. We investigated the effect of Abeta on key transporters of amino acid neurotransmission in cells cultured from rat cerebral cortex. The cultures were treated with Abeta(25-35) at 3 and 10 microM for 12 and 24 h followed by...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9527-2
更新日期：2008-02-01 00:00:00
abstract：:Pre-eclampsia (PE) is a pregnancy complicated syndrome that affects multiple organs including the brain that continue post- delivery in both mother and the offspring. We evaluated the expression of oligodendrocytes in the brain of PE rat model through development as well as the cognitive changes and other behavioural ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-02969-5
更新日期：2020-04-01 00:00:00
abstract：:Glutamate and its receptors are expressed very early during development and may play important roles in neurogenesis, synapse formation and brain wiring. The levels of glutamate and activity of its receptors can be influenced by exogenous factors, leading to neurodevelopmental disorders. To investigate the role of NMD...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9213-9
更新日期：2007-02-01 00:00:00
abstract：:The presynaptic dopamine (DA) D2 receptor-mediated regulation of ATP-sensitive potassium (K+ATP) channels was examined in slices of the rat caudate-putamen. When slices were incubated with the specific D2 receptor antagonist (-)-sulpiride (SLP), a concentration-dependent increase of extracellular DA release was observ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1026620316090
更新日期：2000-12-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common form of dementia in the aged population. Early-onset familial AD (FAD) involves mutations in a gene on chromosome 21 encoding the amyloid protein precursor or on chromosomes 14 or 1 encoding genes known as presenilins. All mutations examined have been found to increase the p...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022471729291
更新日期：1998-05-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9638-4
更新日期：2008-09-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00970527
更新日期：1987-08-01 00:00:00
abstract：:The tumor suppressor PTEN (phosphatase and tensin homolog deleted on chromosome 10) is not only a protein, but also a lipid phosphatase that can negatively regulate the serine/threonine kinase Akt. It has been reported that PTEN can be regulated by means of phosphorylation. However, whether PTEN can be regulated by an...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-9938-3
更新日期：2009-08-01 00:00:00
abstract：:The current studies focus on what mechanisms regulate the concentration of PLP mRNA in cells. The PLP mRNA is very stable and these studies suggest that its stability is regulated by a trans-acting factor specific to oligodendrocytes. In order to test whether the 3'untranslated region (3'UTR) of the PLP mRNA might reg...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021623700009
更新日期：2002-11-01 00:00:00
abstract：:The finding that ketamine, an anaesthetic, can elicit a rapid antidepressant effect at low doses that lasts for weeks in patients with depression is arguably a major achievement in psychiatry in the last decades. However, the mechanisms of action are unclear. The glutamatergic hypothesis of ketamine action posits that...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-019-02744-1
更新日期：2020-01-01 00:00:00
abstract：:The effect of maternal hypoxia on the modification of the fetal brain cell membrane N-methyl-D-aspartate (NMDA) receptor and its modulatory sites was investigated. Experiments were conducted in pregnant guinea pigs of 60 days of gestation. Guinea pig fetuses were exposed to maternal hypoxia (FiO2 = 7%) for 60 minutes....
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968402
更新日期：1992-12-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1006-3
更新日期：2013-05-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965100
更新日期：1983-04-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1922-0
更新日期：2016-08-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964537
更新日期：1985-06-01 00:00:00
abstract：:Intracerebral folate injections produce convulsions and brain lesions, folic acid itself and tetrahydrofolate being more potent toxins than 5-methyltetrahydrofolate, the primary folate of mammalian extracellular fluids. Folates are known to excite neurons, by unknown mechanisms Folates stimulate GTP binding and GTPase...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973648
更新日期：1990-07-01 00:00:00
abstract：:The lysosomal degradation of several sphingolipids requires the presence of four small glycoproteins called saposins, generated by proteolytic processing of a common precursor, prosaposin. Saposins share several structural properties, including six similarly located cysteines forming three disulfide bridges with the s...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022530508763
更新日期：1999-02-01 00:00:00
abstract：:There is a growing body of evidence confirming the involvement of oxidative stress and inflammation in pathogenesis of schizophrenia. Inter-individual variation in antioxidant capacity caused by different genetic profile could potentially influence patient's susceptibility to oxidative damage. In this study we evaluat...
journal_title：Neurochemical research
pub_type： 杂志文章,随机对照试验
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更新日期：2013-02-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000010450.21586.a6
更新日期：2004-01-01 00:00:00
abstract：:Nitric oxide (NO) regulates numerous physiological process and is the main source of reactive nitrogen species (RNS). NO promotes cell survival, but it also induces apoptotic death having been involved in the pathogenesis of several neurodegenerative diseases. NO and superoxide anion react to form peroxynitrite, which...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1805-9
更新日期：2016-02-01 00:00:00
abstract：:It was found that acute ethanol intoxication caused an imbalance of the neurotransmitters in the CNS: accumulation of GABA and serotonin and depletion of catecholamines. Alcohol depression was characterized by suppression of the evoked potentials of the various rat brain structures. Under chronic ethanol intoxication ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966062
更新日期：1982-11-01 00:00:00
