Abstract:
:Alterations in lysosomal proteases have been implicated in many neurodegenerative diseases. The current study demonstrates a concentration-dependent decrease in PC12 cell viability and transient changes in cystatin C (CYSC), cathepsin B (CATB), cathepsin D (CATD) and caspase-3 following exposure to H2O2. Furthermore, activation of CATD occurred following exposure to H2O2 and cysteine protease suppression, while inhibition of CATD with pepstatin A significantly improved cell viability. Additionally, significant PARP cleavage, suggestive of caspase-3-like activity, was observed following H2O2 exposure, while inhibition of caspase-3 significantly increased cell viability compared to H2O2 administration alone. Collectively, our data suggest that H2O2 induced cell death is regulated at least in part by caspase-3 and CATD. Furthermore, cysteine protease suppression increases CATD expression and activity. These studies provide insight for alternate pathways and potential therapeutic targets of cell death associated with oxidative stress and lysosomal protease alterations.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Lee DC,Mason CW,Goodman CB,Holder MS,Kirksey OW,Womble TA,Severs WB,Palm DEdoi
10.1007/s11064-007-9338-5subject
Has Abstractpub_date
2007-09-01 00:00:00pages
1499-510issue
9eissn
0364-3190issn
1573-6903journal_volume
32pub_type
杂志文章abstract::Calcium-naive synaptosomes were used to assess the effects of divalent cations on [3H]acetylcholine release from rat hippocampal homogenates. Following equilibration with calcium-free buffer (containing 10 microM EGTA), calcium reversibly increased [3H]acetylcholine efflux (up to five-fold) while causing no measurable...
journal_title:Neurochemical research
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abstract::The goal of this study was to examine the responsiveness of an immortalized catecholaminergic neuronal line, 2N27, to various growth factors and identify those which promote catecholaminergic expression. 2N27 is a newly established neural cell line derived from fetal rat mesencephalic tissue and, thus, contains tyrosi...
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更新日期:2017-02-01 00:00:00
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journal_title:Neurochemical research
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00
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doi:10.1007/BF00966312
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