Hydrogen peroxide induces lysosomal protease alterations in PC12 cells.

Abstract:

:Alterations in lysosomal proteases have been implicated in many neurodegenerative diseases. The current study demonstrates a concentration-dependent decrease in PC12 cell viability and transient changes in cystatin C (CYSC), cathepsin B (CATB), cathepsin D (CATD) and caspase-3 following exposure to H2O2. Furthermore, activation of CATD occurred following exposure to H2O2 and cysteine protease suppression, while inhibition of CATD with pepstatin A significantly improved cell viability. Additionally, significant PARP cleavage, suggestive of caspase-3-like activity, was observed following H2O2 exposure, while inhibition of caspase-3 significantly increased cell viability compared to H2O2 administration alone. Collectively, our data suggest that H2O2 induced cell death is regulated at least in part by caspase-3 and CATD. Furthermore, cysteine protease suppression increases CATD expression and activity. These studies provide insight for alternate pathways and potential therapeutic targets of cell death associated with oxidative stress and lysosomal protease alterations.

journal_name

Neurochem Res

journal_title

Neurochemical research

authors

Lee DC,Mason CW,Goodman CB,Holder MS,Kirksey OW,Womble TA,Severs WB,Palm DE

doi

10.1007/s11064-007-9338-5

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

1499-510

issue

9

eissn

0364-3190

issn

1573-6903

journal_volume

32

pub_type

杂志文章
  • Characterization of divalent cation-induced [3H]acetylcholine release from EGTA-treated rat hippocampal synaptosomes.

    abstract::Calcium-naive synaptosomes were used to assess the effects of divalent cations on [3H]acetylcholine release from rat hippocampal homogenates. Following equilibration with calcium-free buffer (containing 10 microM EGTA), calcium reversibly increased [3H]acetylcholine efflux (up to five-fold) while causing no measurable...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00966598

    authors: Vickroy TW,Schneider CJ

    更新日期:1991-10-01 00:00:00

  • Phospholipid requirement of Ca2+-stimulated, Mg2+-dependent ATP hydrolysis in rat brain synaptic membranes.

    abstract::The phospholipid requirement for Ca2+-stimulated, Mg2+-dependent ATP hydrolysis (Ca2+/Mg2+-ATPase) and Mg2+-stimulated ATP hydrolysis (Mg2+-ATPase) in rat brain synaptosomal membranes was studied employing partial delipidation of the membranes with phospholipase A2 (Hog pancreas), phospholipase C (Bacillus cereus) and...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00966224

    authors: Gandhi CR,Ross DH

    更新日期:1986-10-01 00:00:00

  • Harpagophytum procumbens prevents oxidative stress and loss of cell viability in vitro.

    abstract::Harpagophytum procumbens, popularly known as devil's claw, is a plant commonly used in the treatment of diseases of inflammatory origin. The anti-inflammatory effects of H. procumbens have been studied; however, the mechanism of action is not elucidated. It is known that excess of reactive oxygen and nitrogen species ...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-013-1133-x

    authors: Schaffer LF,Peroza LR,Boligon AA,Athayde ML,Alves SH,Fachinetto R,Wagner C

    更新日期:2013-11-01 00:00:00

  • Isolation and characterization of endogenous modulators for GABA system.

    abstract::Pig brain extracts from both soluble and membrane fractions were found to contain potent inhibitors for GABA synthesizing enzyme, GAD, referred to as endogenous GAD inhibitors (EGIs) and for the binding of GABA agonist, muscimol, referred to as muscimol binding inhibitors (MBIs). EGIs and MBIs were first purified thro...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00966871

    authors: Yarom M,Bao J,Tang XW,Wu E,Lee YH,Tsai WH,Wu JY

    更新日期:1992-01-01 00:00:00

  • L-kynurenine: its synthesis and possible regulatory function in brain.

    abstract::One pathway by which tryptophan is metabolized in the brain as well as in the periphery is through cleavage of the indole ring to formylkynurenine and then kynurenine. Indoleamine-2,3-dioxygenase, the enzyme that catalyzes this cleavage, and kynurenine are distributed all across the different anatomic regions of brain...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审

    doi:10.1007/BF00964611

    authors: Gál EM,Sherman AD

    更新日期:1980-03-01 00:00:00

  • Catecholaminergic expression in 2N27 immortal neural cell line is enhanced by glial-derived factors.

    abstract::The goal of this study was to examine the responsiveness of an immortalized catecholaminergic neuronal line, 2N27, to various growth factors and identify those which promote catecholaminergic expression. 2N27 is a newly established neural cell line derived from fetal rat mesencephalic tissue and, thus, contains tyrosi...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1023/a:1022482520358

    authors: Grove J,Kentroti S,Prasad K,Vernadakis A

    更新日期:1997-03-01 00:00:00

  • Uptake and metabolism of serotonin by ependymal primary cultures.

    abstract::Serotonin uptake and metabolism was studied in ependymal primary cultures. Serotonin uptake was facilitated by two different systems, one of which was the neuronal serotonin transporter SERT, exhibiting a Vmax value of 3.8+/-0.1 pmol x min(-1) x (mg protein)(-1) and an apparent Michaelis-Menten constant of 0.41+/-0.03...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1023/b:nere.0000035810.08543.97

    authors: Verleysdonk S,Hamprecht B,Rapp M,Wellard J

    更新日期:2004-09-01 00:00:00

  • Effect of acute administration of L-tyrosine on oxidative stress parameters in brain of young rats.

    abstract::Tyrosinemia type II, also known as Richner-Hanhart syndrome, is an autosomal recessive inborn error of metabolism caused by a deficiency of hepatic cytosolic tyrosine aminotransferase, and is associated with neurologic and development difficulties in numerous patients. Considering that the mechanisms underlying the ne...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-013-1180-3

    authors: Macêdo LG,Carvalho-Silva M,Ferreira GK,Vieira JS,Olegário N,Gonçalves RC,Vuolo FS,Ferreira GC,Schuck PF,Dal-Pizzol F,Streck EL

    更新日期:2013-12-01 00:00:00

  • Metabolism, compartmentation, transport and production of acetate in the cortical brain tissue slice.

    abstract::Acetate is a two carbon intermediate in metabolism. It is an accepted marker of astrocytic metabolism, and a substrate for production of metabolites such as glutamine, glutamate and GABA. However, anomalies exist in the current explanations of compartmentation and metabolism of acetate. Here, we investigated these ano...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审

    doi:10.1007/s11064-012-0847-5

    authors: Rae C,Fekete AD,Kashem MA,Nasrallah FA,Bröer S

    更新日期:2012-11-01 00:00:00

  • Characterization of endogenous amino acid efflux from hippocampal slices during chemically-induced ischemia.

    abstract::Using sodium (NaN3)-induced anoxia plus aglycaemia as a model of chemically-induced ischemia, we have characterized the endogenous release of excitatory and inhibitory amino acids from superfused hippocampal slices. Chemical ischemia produced an azide (1-30 mM) dose-dependent increase in the efflux of glutamate, aspar...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1023/a:1011094728469

    authors: Djali S,Dawson LA

    更新日期:2001-02-01 00:00:00

  • Comparative study of miscellaneous properties of cysteine sulfinate transaminase and glutamate oxaloacetate transaminase in chick retina homogenate.

    abstract::The activity, properties, and developmental pattern of cysteine sulfinate transaminase (CSA-T) were studied in chick retina and compared with the activity, properties, and developmental pattern of glutamate oxaloacetate transaminase (GOT). Their optimum pH is identical whereas the effect of pyridoxal phosphate seems t...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00964358

    authors: Recasens M,Gabellec MM,Mack G,Mandel P

    更新日期:1978-02-01 00:00:00

  • Sphingolipid metabolism and caveolin expression in gonadotropin-releasing hormone-expressing GN11 and gonadotropin-releasing hormone-secreting GT1-7 neuronal cells.

    abstract::In this paper, we show that caveolin-1 is abundantly present in a cell line of immortalized gonadotropin-releasing hormone-expressing neurons (GN11). In contrast to GN11, caveolin is undetectable in a cognate cell line of immortalized gonadotropin-releasing hormone-secreting neurons (GT1-7). These two cell lines are c...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1023/a:1020217309987

    authors: Prioni S,Loberto N,Prinetti A,Chigorno V,Guzzi F,Maggi R,Parenti M,Sonnino S

    更新日期:2002-08-01 00:00:00

  • Antidepressant-Like Effect of Isorhynchophylline in Mice.

    abstract::Isorhynchophylline (IRN), an oxindole alkaloid, has been identified as the main active ingredient responsible for the biological activities of Uncaria rhynchophylla (Miq) Miq ex Havil. (Rubiaceae). Previous studies in our laboratory have revealed that IRN possesses potent neuroprotective effects in different models of...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-016-2124-5

    authors: Xian YF,Fan D,Ip SP,Mao QQ,Lin ZX

    更新日期:2017-02-01 00:00:00

  • Functional analysis for peripheral myelin protein PASII/PMP22: is it a member of claudin superfamily?

    abstract::Two major glycoproteins, P0 and PASII/PMP22, are specifically expressed in peripheral myelin. Point mutations of these proteins and over or under expression of PASII/PMP22 cause various hereditary peripheral neuropathies. P0 is well characterized as a major adhesion molecule in PNS myelin, but the function of PASII/PM...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1023/a:1010927001378

    authors: Takeda Y,Notsu T,Kitamura K,Uyemura K

    更新日期:2001-06-01 00:00:00

  • Differential involvement of intracellular Ca2+ in 1-methyl-4-phenylpyridinium- or 6-hydroxydopamine-induced cell viability loss in PC12 cells.

    abstract::1-Methyl-4-phenylpyridinium (MPP(+)) or 6-hydroxydopamine (6-OHDA) caused a nuclear damage, the mitochondrial membrane permeability changes, leading to the cytochrome c release and caspase-3 activation, the formation of reactive oxygen species and the depletion of GSH in PC12 cells. Nicardipine (a calcium channel bloc...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-006-9088-9

    authors: Lee DH,Han YS,Han ES,Bang H,Lee CS

    更新日期:2006-07-01 00:00:00

  • Ifenprodil Attenuates Methamphetamine-Induced Behavioral Sensitization and Activation of Ras-ERK-∆FosB Pathway in the Caudate Putamen.

    abstract::Addiction is a debilitating, chronic psychiatric disorder that is difficult to cure completely owing to the high rate of relapse. Behavioral sensitization is considered to may underlie behavioral changes, such as relapse, caused by chronic abuse of psychomotor stimulants. Thus, its animal models have been widely used ...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-016-1976-z

    authors: Li L,Liu X,Qiao C,Chen G,Li T

    更新日期:2016-10-01 00:00:00

  • Long-term support by injured brain extract of a subpopulation of ciliary ganglion neurons purified by differential adhesion.

    abstract::Ciliary ganglion neurons and nonneurons can be separated from each other, based on the greater adhesivity of the nonneurons to untreated tissue culture plastic in the presence of serum. When the separation was carried out in the presence of Serum Plus (a commercially available supplemented serum), two populations of n...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00966312

    authors: Needels DL,Nieto-Sampedro M,Cotman CW

    更新日期:1987-10-01 00:00:00

  • Assessment of the efficacy of solutes from extremophiles on protein aggregation in cell models of Huntington's and Parkinson's diseases.

    abstract::Protein misfolding and deposition in the brain are implicated in the etiology of numerous neurodegenerative disorders. Here, organic solutes characteristic of microorganisms adapted to hot environments, were tested on experimental cell models of Huntington's and Parkinson's diseases. Diglycerol phosphate, di-myo-inosi...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-011-0440-3

    authors: Jorge CD,Ventura R,Maycock C,Outeiro TF,Santos H,Costa J

    更新日期:2011-06-01 00:00:00

  • Chlorpyrifos induced region specific vulnerability in rat CNS and modulation by age and cold stress: an interactive study.

    abstract::Chlorpyrifos (CPF), an organophosphorus insecticide is known to cause ill health in non-target animals by inducing oxidative stress. In this study influence of cold stress (15°C and 20°C) and age as modulating factors on CPF induced oxidative stress was addressed to assess age-related differences and vulnerability in ...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-010-0311-3

    authors: Basha PM,Poojary A

    更新日期:2011-02-01 00:00:00

  • Neurochemical effects of prenatal treatment with ochratoxin A on fetal and adult mouse brain.

    abstract::Ochratoxin A (OA) is a mycotoxin produced by several storage fungi, such as Aspergillus ochraceus and several Penicillium species. OA (3 mg/kg) was given intraperitoneally to pregnant mice on day 11 of gestation (day 1 = day of insemination), and neurochemical changes in brains of their offspring were examined at feta...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00971631

    authors: Tamaru M,Hirata Y,Matsutani T

    更新日期:1988-12-01 00:00:00

  • Acetylcholinesterase inhibitor ENA-713 protects against ischemia-induced decrease in pre- and postsynaptic cholinergic indices in the gerbil brain following transient ischemia.

    abstract::The effects of pre-treatment with ENA-713, an acetylcholinesterase (AChE) inhibitor, on changes in pre- and postsynaptic cholinergic indices in gerbil brain following transient ischemia were studied at 4 and 14 days after recirculation. In the ischemic group, hippocampal acetylcholine (ACh) level was significantly red...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00966804

    authors: Tanaka K,Ogawa N,Mizukawa K,Asanuma M,Kondo Y,Nishibayashi S,Mori A

    更新日期:1994-02-01 00:00:00

  • Neurite outgrowth in PC12 cells stimulated by acetyl-L-carnitine arginine amide.

    abstract::Senescence of the central nervous system is characterized by a progressive loss of neurons that can result in physiological and behavioral impairments. Reduction in the levels of central neurotrophic factors or of neurotrophin receptors may be one of the causes of the onset of these degenerative events. Thus, a proper...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00995145

    authors: Taglialatela G,Navarra D,Olivi A,Ramacci MT,Werrbach-Perez K,Perez-Polo JR,Angelucci L

    更新日期:1995-01-01 00:00:00

  • Regional vulnerability to oxidative stress in a model of experimental epilepsy.

    abstract::We evaluated oxidative stress associated with a model of experimental epilepsy. Male Wistar rats were injected i.p. with 150 mg/kg convulsant 3-mercaptopropionic acid and decapitated in two stages: during seizures or in the post-seizure period. Spontaneous chemiluminescence, levels of thiobarbituric acid reactive subs...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF03177490

    authors: Lores Arnaiz S,Travacio M,Llesuy S,Rodríguez de Lores Arnaiz G

    更新日期:1998-12-01 00:00:00

  • Polysomes during early postnatal development of brain in the rat.

    abstract::We have attempted to show eventual modifications in the brain protein synthesis apparatus of rat during the first three weeks after birth. Through this time we noted a steady decrease (about 60%) in the free polysomes, when expressed relative to tissue weight. This decrease does not correlate with changes in the polys...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00966130

    authors: Gauthier D,Ven Murthy MR

    更新日期:1986-09-01 00:00:00

  • Neurochemical changes in rat striatum and substantia nigra following drug administration : Evidence for an amino acid-dopamine-acetylcholine link.

    abstract::The effects of a number of drugs that deplete catecholamines or block their receptors were investigated on the levels of free amino acids and glutamate decarboxylase activity in rat striatum and substantia nigra, in an attempt to clarify the possible links between catecholamine and amino acid transmitter function. Whi...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00966009

    authors: Roberts PJ,Kapur H

    更新日期:1977-10-01 00:00:00

  • The Antidepressant Effects of Resveratrol are Accompanied by the Attenuation of Dendrite/Dendritic Spine Loss and the Upregulation of BDNF/p-cofilin1 Levels in Chronic Restraint Mice.

    abstract::Depression afflicts more than 300 million people worldwide, but there is currently no universally effective drug in clinical practice. In this study, chronic restraint stress (CRS)-induced mice depression model was used to study the antidepressant effects of resveratrol and its mechanism. Our results showed that resve...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-020-03200-1

    authors: Chen JJ,Shen JX,Yu ZH,Pan C,Han F,Zhu XL,Xu H,Xu RT,Wei TY,Lu YP

    更新日期:2021-01-03 00:00:00

  • Post Translational Modulation of β-Amyloid Precursor Protein Trafficking to the Cell Surface Alters Neuronal Iron Homeostasis.

    abstract::Cell surface β-Amyloid precursor protein (APP) is known to have a functional role in iron homeostasis through stabilising the iron export protein ferroportin (FPN). Mechanistic evidence of this role has previously only been provided through transcriptional or translational depletion of total APP levels. However, numer...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-019-02747-y

    authors: Tsatsanis A,Dickens S,Kwok JCF,Wong BX,Duce JA

    更新日期:2019-06-01 00:00:00

  • Müller Cells as a source of brain-derived neurotrophic factor in the retina: noradrenaline upregulates brain-derived neurotrophic factor levels in cultured rat Müller cells.

    abstract::Müller cells, the predominant glial cells in the retina, are thought to play important roles in the survival of retinal neurons. Previous studies have demonstrated that Müller cells express brain-derived neurotrophic factor (BDNF), which has a pronounced neurotrophic effect on retinal ganglion cells. In this study, we...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-005-7936-7

    authors: Seki M,Tanaka T,Sakai Y,Fukuchi T,Abe H,Nawa H,Takei N

    更新日期:2005-09-01 00:00:00

  • Effects of aging on the interaction of quinuclidinyl benzilate, N-methylscopolamine, pirenzepine, and gallamine with brain muscarinic receptors.

    abstract::The objective of the present study was to investigate the effects of senescence on the binding characteristics of muscarinic receptors by using [3H]quinuclidinyl benzilate ([3H]QNB) and [3H]N-methylscopolamine ([3H]NMS) as ligands in young (3 months), middle-age (10 months) and old (24 months) male Fischer 344 rats. M...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00971637

    authors: Surichamorn W,Kim ON,Lee NH,Lai WS,el-Fakahany EE

    更新日期:1988-12-01 00:00:00

  • Arginine specific endopeptidases modify the aggregation properties of a synthetic peptide derived from Alzheimer beta/A4 amyloid.

    abstract::A synthetic peptide corresponding to the first 28 amino acids of the Alzheimer disease amyloid beta/A4 peptide (3.2 kDa) aggregated to a high molecular weight (15 kDa) on SDS/urea polyacrylamide gels. Proteinase K, V8 protease, trypsin, and endopeptidase Lys-C readily degraded the aggregate. By contrast, when digested...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00974579

    authors: Honda T,Marotta CA

    更新日期:1992-04-01 00:00:00