Abstract：
:Intracerebral folate injections produce convulsions and brain lesions, folic acid itself and tetrahydrofolate being more potent toxins than 5-methyltetrahydrofolate, the primary folate of mammalian extracellular fluids. Folates are known to excite neurons, by unknown mechanisms Folates stimulate GTP binding and GTPase activity in slime molds. We observed folate stimulation of GTP gamma S binding and inhibition of high affinity GTPase activity in rat brain membranes. Three fold stimulation of GTP gamma S binding was observed in cerebellar membranes treated with 50 microM FA. Folic acid (FA), dihydrofolate (DHF) and tetrahydrofolate (THF) were much more potent than 5-methyltetrahydrofolate in this regard. The effect varies between brain regions and was greatest in cerebellar and hippocampal membranes. Folates inhibit GTPase activity, with DHF and FA being the most potent and maximum inhibition being to 33% of control values. We find high affinity guanine nucleotide sensitive binding of [3H]FA in cerebellar membranes, another response typical of G protein coupled membrane receptors. Folates were also shown to stimulate the release of [3H]GDP from brain membranes. These effects are seen in washed brain membranes and can not be explained by any known folate metabolic or coenzyme functions. They resemble the effects of cholera toxin, except for their reversibility. They may be relevant to known folate neuroexcitant effects of folates.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Hartley DM,Snodgrass SRdoi
10.1007/BF00973648subject
Has Abstractpub_date
1990-07-01 00:00:00pages
681-6issue
7eissn
0364-3190issn
1573-6903journal_volume
15pub_type
杂志文章abstract：:The effect of light exposure on the protein patterns of optic lobe and forebrain of the chick embryo was analysed by a high-resolution micro-two-dimensional polyacrylamide gel electrophoresis and computerized quantitation. Experiments were done on three groups of eggs: control group was incubated in the dark; simultan...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00993240
更新日期：1987-04-01 00:00:00
abstract：:Studies on primary astrocytes cultured in vitro have shown that process formation involves changes in cytoskeletal proteins and release of tension on the substratum. Actin filament reorganization has previously been found to be the major cytoskeletal change occurring during process formation. These changes are relativ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022413718447
更新日期：1998-03-01 00:00:00
abstract：:Oxidative stress-induced neuronal cell death requires opening of the mitochondrial permeability transition pore. P53 mitochondrial translocation and association with Cyclophilin D (Cyp-D) is required for the pore opening. Here we tested this signaling axis in oxygen glucose deprivation (OGD)/re-oxygenation-induced in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-0968-5
更新日期：2013-04-01 00:00:00
abstract：:Endogenous phosphorylation of proteins from rat brain synaptosomal plasma membranes was studied in vitro. Cyclic AMP (cAMP) markedly stimulated(32)P incorporation in three protein bands with molecular weights of 75,000, 57,000, and 54,000, respectively. The effect of the behaviorally active peptide ACTH1-24 on this en...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965607
更新日期：1976-12-01 00:00:00
abstract：:N-Methyl-D-aspartate (NMDA) and L-glutamate activate membrane receptors that produce substantial permeation of Na+, K+ and Ca2+ through the neuronal membrane. These ionic fluxes are intimately linked to processes that regulate neuronal survival, growth and differentiation. Intracellular free Ca2+ concentrations are th...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00966862
更新日期：1992-01-01 00:00:00
abstract：:A concise discussion of the role of different geometrical conformational states in the process of self-assembling of gangliosides is given. The report focuses on the effects of the geometrical variations occurring in the head group region of gangliosides as reflected on the geometrical properties of the whole assembly...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1020255529125
更新日期：2002-08-01 00:00:00
abstract：:Metabolic turnover of axonally transported glycoproteins has been examined in membranous and soluble subfractions of goldfish optic tectum following intraocular injection of [3H]fucose. Radioactivity in total transported glycoproteins reached a maximum in the tectum after 24-30 hr, then declined with a half-life of ap...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964224
更新日期：1980-04-01 00:00:00
abstract：:(4S)-4-Amino-5,6-heptadienoic acid [S)-gamma-allenyl-GABA; MDL 72483) is a potent inactivator of brain GABA-T in mice; (ED50 (i.p.) = 60 mg.kg-1; ED50 (oral) = 70 mg.kg-1). Its anticonvulsant effects against 3-mercaptopropionic acid (MPA)-induced seizures in mice is related to the elevation of whole brain GABA concent...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966092
更新日期：1991-03-01 00:00:00
abstract：:The diversification of neuronal subtypes during corticogenesis is fundamental to the establishment of the complex cortical structure. Although subtype specification has been assumed to occur in neural progenitor cells, increasing evidence has begun to reveal the plasticity of subtype determination in immature neurons....
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2441-3
更新日期：2018-01-01 00:00:00
abstract：:The modulation of intracellular GABA activated 36Cl- in-->out permeation across single Deiters' neuron membranes has been studied in a microchamber system. Addition of Mg2+/ATP on the membrane cytoplasmic side reduces strongly the GABA effect as does ATP alone. However, the greatest inhibition of the GABA effect is gi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021073230464
更新日期：1999-08-01 00:00:00
abstract：:It is thought that changes in gene expression in the brain mediate chronic ethanol-induced complex behaviors such as tolerance, dependence, and sensitization, and also relate to ethanol-induced brain toxicity. Using high-density filter-based cDNA microarrays (GeneFilters), we analyzed the expression of over 5000 genes...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020937728506
更新日期：2002-10-01 00:00:00
abstract：:A role for protein phosphorylation in the process of neurite outgrowth has been inferred from many studies of the effects of protein kinase inhibitors and activators on cultured neurotumor cells and primary neuronal cells from developing brain or ganglia. Here we re-examine this issue, using a culture system derived f...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021916509858
更新日期：1997-10-01 00:00:00
abstract：:The effects of phenylalanine (PHE) and its deaminated metabolites phenylpyruvate (PHP), phenyllactate (PHL) and phenylacetate (PHA) on sodium and potassium activated adenosinetriphosphatase (Na+,K+-ATPase) in synaptosomes from rat brain were investigated. At very low concentrations (5-10 microM). PHE, PHL and PHA inhi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965524
更新日期：1982-06-01 00:00:00
abstract：:Altered neurogenesis has been reported in Alzheimer disease (AD), the most common neurodegenerative disorder characterized with hyperphosphorylated tau and accumulation of β-amyloid (Aβ). Recent studies suggest that tau phosphorylation is essential for hippocampal neurogenesis, however, it is not known whether tau pho...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0316-y
更新日期：2011-02-01 00:00:00
abstract：:The presynaptic dopamine (DA) D2 receptor-mediated regulation of ATP-sensitive potassium (K+ATP) channels was examined in slices of the rat caudate-putamen. When slices were incubated with the specific D2 receptor antagonist (-)-sulpiride (SLP), a concentration-dependent increase of extracellular DA release was observ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1026620316090
更新日期：2000-12-01 00:00:00
abstract：:Ion fluxes in mammalian myelinated axons are restricted to the nodes of Ranvier, where, in particular, voltage-gated Na+ channels are clustered at a high density. The node of Ranvier is separated from the internode by two distinct domains of the axolemma, the paranode and the juxtaparanode. Each axonal domain is chara...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021608514646
更新日期：2003-01-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common form of dementia that progressively disrupts neurocognitive function, which has neither cure nor effective treatment. Hypercholesterolemia might be involved in brain alterations that could evolve into AD. The present study aims to evaluate the potential of omega-3, Co-enzyme...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-02996-2
更新日期：2020-05-01 00:00:00
abstract：:This investigation was performed to evaluate the effects of ACPD [(1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid], a metabotropic glutamate receptor agonist, on cerebral O2 consumption during focal cerebral ischemia. Male Wistar rats were placed in control (n = 7) and ACPD (n = 7) groups under isoflurane anesthesia...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1026159422237
更新日期：2003-12-01 00:00:00
abstract：:Chlorpyrifos (CPF), an organophosphorus insecticide is known to cause ill health in non-target animals by inducing oxidative stress. In this study influence of cold stress (15°C and 20°C) and age as modulating factors on CPF induced oxidative stress was addressed to assess age-related differences and vulnerability in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0311-3
更新日期：2011-02-01 00:00:00
abstract：:SENP3 (SUMO-specific proteases 3), a member of the small ubiquitin-like modifier specific protease family, was identified as a molecule that deconjugates SUMOylation of modified protein substrates and functions as an isopeptidase by disrupting SUMO homeostasis to facilitate cancer development and progression. However,...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0869-z
更新日期：2012-12-01 00:00:00
abstract：:The present study addressed the hypothesis that the DA transporter ligand, [3H]mazindol, labels multiple sites/states associated with the dopamine (DA) transporter in striatal membranes. Incubations with [3H]mazindol proceeded for 18-24 hr at 4 degrees C in 55.2 mM sodium phosphate buffer, pH 7.4, with a protease inhi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966817
更新日期：1994-02-01 00:00:00
abstract：:Neuroprotectin D1 (NPD1), a docosahexaenoic acid (DHA)-derived lipid mediator, promotes survival in cells exposed to oxidative stress by inducing the activity of anti-inflammatory mediators and suppressing the expression of pro-inflammatory genes. Though retinal pigment epithelial (RPE) cells naturally produce NPD1 fr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0351-8
更新日期：2010-12-01 00:00:00
abstract：:Endogenous and xenobiotic sulphur-containing convulsant and non-convulsant compounds containing structural moieties of, or bearing a structural resemblance to, GABA and homocysteine were tested in binding studies for their potency in displacing the GABA-mimetic [3H]muscimol from specific, high-affinity sites (Kd = 3.6...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00971006
更新日期：1987-07-01 00:00:00
abstract：:In the present study, we aimed to investigate the effects of pinealectomy and chronic melatonin administration on focal epileptiform activity induced by penicillin in the rat cortex and to determine the relation between melatonin levels and electrocorticogram (ECoG) power spectrum. For this purpose, male Sprague-Dawle...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1069-1
更新日期：2013-08-01 00:00:00
abstract：:Mouse isolated vas deferens preincubated with [3-H]noradrenaline was superfused and the effect of alpha 1-adrenoceptor agonists was studied on the release of total radioactivity ([3H]noradrenaline + 3H-metabolites) and [3H]noradrenaline. Reverse phase high pressure liquid chromatography (HPLC) combined with scintillat...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965167
更新日期：1986-01-01 00:00:00
abstract：:Mammalian MTH1 protein, a MutT-related protein, catalyzes the hydrolysis of 8-oxo-7,8-dihydroguanosine triphosphate (8-oxoGTP) to monophosphate, thereby preventing incorporation of 8-oxo-7,8-dihydroguanine (8-oxoguanine) into RNA. In this study, we applied immunohistochemistry to follow the expression of MTH1 and the ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0484-4
更新日期：2011-08-01 00:00:00
abstract：:The effects of 100microg melatonin injection at postnatal day 5 (PD 5) on the development of the central serotonergic systems in male and female rats were investigated. The contents of serotonin (5-HT) and 5-hydroxy-3-indolacetic acid (5-HIAA) were measured in several extrahypothalamic regions at 3, 10 and 42 weeks of...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007517524179
更新日期：2000-06-01 00:00:00
abstract：:Recent evidence reveals that prolactin gene expression (PRL-GE) in mammotropes occurs in pulses, but the molecular process(es) underlying this phenomenon remains unclear. Earlier, we have identified an E-box (E-box133) in the rat PRL promoter that binds several circadian elements and is critical for this dynamic proce...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1843-y
更新日期：2016-06-01 00:00:00
abstract：:Memory impairment is the most common symptom in patients with Alzheimer's disease. The purpose of this study is to evaluate the memory enhancing effects of P7C3, a recently identified compound with robust proneurogenic and neuroprotective effects, on the cognitive impairment induced by scopolamine, a muscarinic acetyl...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1783-y
更新日期：2016-05-01 00:00:00
abstract：:We have previously showed that peptide neurotensin inhibits neuronal Na(+), K(+)-ATPase activity, an effect which involves high affinity neurotensin receptor. Nitric oxide (NO) acts as a neurotransmitter or as a neuromodulator when it is synthesized by neuronal nitric oxide synthase. Neurotensin effect on Na(+), K(+)-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0552-9
更新日期：2011-12-01 00:00:00
