NPD1 induction of retinal pigment epithelial cell survival involves PI3K/Akt phosphorylation signaling.


:Neuroprotectin D1 (NPD1), a docosahexaenoic acid (DHA)-derived lipid mediator, promotes survival in cells exposed to oxidative stress by inducing the activity of anti-inflammatory mediators and suppressing the expression of pro-inflammatory genes. Though retinal pigment epithelial (RPE) cells naturally produce NPD1 from DHA, investigating the mechanisms through which exogenous NPD1 induces cell survival is essential to assess mechanisms of actions and the potential of this lipid mediator for treatment of retinal degenerative diseases. The PI3K/Akt and mTOR/p70S6K pathways are responsible for supporting cell survival upon exposure to oxidative stress. In human ARPE-19 cells pretreated with NPD1 then exposed to varying concentrations of oxidative stress or repeated exposures to oxidative stress, Akt, mTOR, and p70S6K were phosphorylated to a greater extent and for a greater duration than cells not pretreated with NPD1. In addition to increased phosphorylation, a subsequent decreased rate of apoptosis was observed upon NPD1 treatment. Thus NPD1 bioactivity in RPE cells enhances activation of these pathways and promotes cell integrity and survival.


Neurochem Res


Neurochemical research


Halapin NA,Bazan NG




Has Abstract


2010-12-01 00:00:00












  • [3H]muscimol and [3H]flunitrazepam binding sites in the developing cerebellum of mice treated with methylazoxymethanol at different postnatal ages.

    abstract::Two models of perturbed cerebellar ontogenesis were obtained by a single administration of methylazoxymethanol (MAM), a potent antimitotic agent, to mouse pups either on the day of birth (MAM0 mice) or at postnatal day 5 (MAM5 mice). The alterations of the cerebellar GABAergic system were studied by measuring glutamic...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Bacon E,Girard C,de Barry J,Gombos G

    更新日期:1992-07-01 00:00:00

  • Partial purification of a phosphoethanolamine methyltransferase from rat brain cytosol.

    abstract::The conversion of phosphoethanolamine to phosphocholine requires 3 separate N-methyltransferases. We had previously purified the enzyme catalyzing the last methylation, phosphodimethylethanolamine N-methyltransferase. We have successfully purified the enzyme catalyzing the initial methylation of phosphoethanolamine. A...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Mukherjee S,Freysz L,Kanfer JN

    更新日期:1995-10-01 00:00:00

  • Phospholipid requirement of Ca2+-stimulated, Mg2+-dependent ATP hydrolysis in rat brain synaptic membranes.

    abstract::The phospholipid requirement for Ca2+-stimulated, Mg2+-dependent ATP hydrolysis (Ca2+/Mg2+-ATPase) and Mg2+-stimulated ATP hydrolysis (Mg2+-ATPase) in rat brain synaptosomal membranes was studied employing partial delipidation of the membranes with phospholipase A2 (Hog pancreas), phospholipase C (Bacillus cereus) and...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Gandhi CR,Ross DH

    更新日期:1986-10-01 00:00:00

  • Uptake of acetyl-L-carnitine in the brain.

    abstract::Analysis in mouse brain slices of the uptake of acetyl-L-[N-methyl-14C]carnitine with time showed it to be concentrative, and kinetic analysis gave a Km of 1.92 mM and a Vmax of 1.96 mumol/min per ml, indicating the presence of a low-affinity carrier system. The uptake was energy-requiring and sodium-dependent, being ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Burlina AP,Sershen H,Debler EA,Lajtha A

    更新日期:1989-05-01 00:00:00

  • Effects of glia maturation factor overexpression in primary astrocytes on MAP kinase activation, transcription factor activation, and neurotrophin secretion.

    abstract::Using the replication-defective adenovirus vector, we overexpressed rat glia maturation factor (GMF) in primary astrocyte cultures derived from embryonic rat brains. Among the three isoforms of MAP kinase, there was a big increase in the phosphorylation of p38, as detected with Western blotting using the phosphospecif...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Zaheer A,Yorek MA,Lim R

    更新日期:2001-12-01 00:00:00

  • The unfolded protein response is triggered in rat neurons of the dorsal raphe nucleus after single-prolonged stress.

    abstract::The dorsal raphe nucleus (DRN) has been suggested playing an important role in the pathophysiology of post-traumatic stress disorder (PTSD), however the underlying cellular mechanisms are not fully understood. The endoplasmic reticulum (ER) is a critical organelle for synthesis of membrane and secretory proteins, and ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Xie J,Han F,Shi Y

    更新日期:2014-04-01 00:00:00

  • Supersensitivity of GABA-A receptors in hepatic encephalopathy.

    abstract::During the past decade a new approach to pathogenetic studies of hepatic encephalopathy has been undertaken to identify the neurochemical alterations which characterize the syndrome. Using animal models of hepatic encephalopathy electrophysiological, behavioral, pharmacological and biochemical evidence were provided o...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: Baraldi M

    更新日期:1990-02-01 00:00:00

  • GABA metabolism in Venezuelan equine encephalomyelitis virus infection.

    abstract::Mice infected with the Venezuelan equine encephalomyelitis virus showed a significant decrease in the GABA content of cerebral hemispheres. Activity of the enzyme which synthetizes GABA, glutamate decarboxylase, is also reduced in whole cerebral hemispheres, neostriatum, and frontal cortex of infected animals, as comp...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Bonilla E,Ryder E,Ryder S

    更新日期:1980-02-01 00:00:00

  • Inhibition of nitric oxide synthase with 7-nitroindazole does not modify early metabolic recovery following focal cerebral ischemia in rats.

    abstract::Nitric oxide has been strongly implicated in the development of tissue infarction in response to focal cerebral ischemia. Nitric oxide and its derivatives can inhibit components of the electron transport chain, providing a likely target for these substances in ischemic and post-ischemic brain. Lactate content is incre...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Helps SC,Sims NR

    更新日期:2007-04-01 00:00:00

  • Inhibitory avoidance learning inhibits ectonucleotidases activities in hippocampal synaptosomes of adult rats.

    abstract::Several lines of evidence indicate that ATP may play an important role in Long-Term Potentiation. In this investigation we evaluated the effect of a memory task (step-down inhibitory avoidance) on the synaptosomal ecto-enzymes (ATP diphosphohydrolase and 5'-nucleotidase) involved in the degradation of ATP to adenosine...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Bonan CD,Dias MM,Battastini AM,Dias RD,Sarkis JJ

    更新日期:1998-07-01 00:00:00

  • Acetylcholinesterase inhibitor ENA-713 protects against ischemia-induced decrease in pre- and postsynaptic cholinergic indices in the gerbil brain following transient ischemia.

    abstract::The effects of pre-treatment with ENA-713, an acetylcholinesterase (AChE) inhibitor, on changes in pre- and postsynaptic cholinergic indices in gerbil brain following transient ischemia were studied at 4 and 14 days after recirculation. In the ischemic group, hippocampal acetylcholine (ACh) level was significantly red...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Tanaka K,Ogawa N,Mizukawa K,Asanuma M,Kondo Y,Nishibayashi S,Mori A

    更新日期:1994-02-01 00:00:00

  • Neurotensin effect on Na+, K+-ATPase is CNS area- and membrane-dependent and involves high affinity NT1 receptor.

    abstract::We have previously shown that peptide neurotensin inhibits cerebral cortex synaptosomal membrane Na+, K+-ATPase, an effect fully prevented by blockade of neurotensin NT1 receptor by antagonist SR 48692. The work was extended to analyze neurotensin effect on Na+, K+-ATPase activity present in other synaptosomal membran...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: López Ordieres MG,Rodríguez de Lores Arnaiz G

    更新日期:2002-11-01 00:00:00

  • Analysis and separation of synaptosomal membrane proteins.

    abstract::Synaptosomal membrane proteins solubilized with 8% CHAPS-8 M urea were analyzed with two-dimensional electrophoresis (2DE). The membrane proteins were resolved up to 250 spots on a 2DE map, ranging in isoelectric points (pI) from 3.5 to 10.0 and molecular weights (MW) from 10 kDa to 200 kDa. Comparison of the mapped p...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Ishioka N,Oda T,Natake Y,Kurioka S

    更新日期:1990-05-01 00:00:00

  • Human Remyelination Promoting Antibody Stimulates Astrocytes Proliferation Through Modulation of the Sphingolipid Rheostat in Primary Rat Mixed Glial Cultures.

    abstract::Remyelination promoting human IgMs effectively increase the number of myelinated axons in animal models of multiple sclerosis. Hence, they ultimately stimulate myelin production by oligodendrocytes (OLs); however, their exact mechanism of action remains to be elucidated, and in particular, it remains unclear whether t...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Grassi S,Giussani P,Prioni S,Button D,Cao J,Hakimi I,Sarmiere P,Srinivas M,Cabitta L,Sonnino S,Prinetti A

    更新日期:2019-06-01 00:00:00

  • Interaction of adenosine and guanine derivatives in the rat hippocampus: effects on cyclic AMP levels and on the binding of adenosine analogues and GMP.

    abstract::Guanine nucleotides (GN) have been implicated in many intracellular mechanisms. Extracellular actions, probably as glutamate receptor antagonists, have also been recently attributed to these compounds. GN may have a neuroprotective role by inhibiting excitotoxic events evoked by glutamate. Effects of extracellular GN ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Tasca CI,Souza DO

    更新日期:2000-02-01 00:00:00

  • Acrylamide Retards the Slow Axonal Transport of Neurofilaments in Rat Cultured Dorsal Root Ganglia Neurons and the Corresponding Mechanisms.

    abstract::Chronic acrylamide (ACR) exposure induces peripheral-central axonopathy in occupational workers and laboratory animals, but the underlying mechanisms remain unclear. In this study, we first investigated the effects of ACR on slow axonal transport of neurofilaments in cultured rat dorsal root ganglia (DRG) neurons thro...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: An L,Li G,Si J,Zhang C,Han X,Wang S,Jiang L,Xie K

    更新日期:2016-05-01 00:00:00

  • Effect of Variable Doses of Zinc Oxide Nanoparticles on Male Albino Mice Behavior.

    abstract::Zinc oxide nanoparticles (ZnO NPs) have diverse utility these days ranging from being part of nanosensors to be ingredient of cosmetics. Present study was designed to report the effect of variable doses of ZnO NPs on selected aspects of male albino mice behavior. Nano particles were synthesized by sol-gel auto-combust...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Zahra J,Iqbal S,Zahra K,Javed Z,Shad MA,Akbar A,Ashiq MN,Iqbal F

    更新日期:2017-02-01 00:00:00

  • L-kynurenine: its synthesis and possible regulatory function in brain.

    abstract::One pathway by which tryptophan is metabolized in the brain as well as in the periphery is through cleavage of the indole ring to formylkynurenine and then kynurenine. Indoleamine-2,3-dioxygenase, the enzyme that catalyzes this cleavage, and kynurenine are distributed all across the different anatomic regions of brain...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: Gál EM,Sherman AD

    更新日期:1980-03-01 00:00:00

  • Dissection of calcium signaling events in exocrine secretion.

    abstract::The secretion of fluid and electrolytes by salivary gland acinar cells requires the coordinated regulation of multiple ion channel and transporter proteins, signaling components, and water transport. Importantly, neurotransmitter stimulated increase in the cytosolic free [Ca(2+)] ([Ca(2+)](i)) is critical for the regu...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: Ambudkar IS

    更新日期:2011-07-01 00:00:00

  • Influence of intraventricular application of baclofen on arterial blood pressure and neurotransmitter concentrations in the hypothalamic paraventricular nucleus of rats.

    abstract::The hypothalamic paraventricular nucleus (PVN) is a key site for regulating neuroendocrine functions in the magnocellular part and autonomic activities in the parvocellular part. Its anatomical proximity to the third ventricle could be a good target for intrathecal injection of baclofen. We investigated the correlatio...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Czell D,Efe T,Preuss M,Schofer MD,Becker R

    更新日期:2012-02-01 00:00:00

  • Increased seizure susceptibility induced by guanidinoethane sulfonate in E1 mice and its relation to glutamatergic neurons.

    abstract::Convulsions and brain levels of amino acids and 5-hydroxytryptamine (5-HT) in E1 mice were examined after oral administration of a 1% guanidinoethane sulfonate (GES) solution. The incidence of convulsions increased 3 days after starting GES administration, and this effect continued throughout the 6 months of drug admi...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Hiramatsu M,Edamatsu R,Kabuto H,Higashihara Y,Mori A

    更新日期:1989-01-01 00:00:00

  • Protective effects of hypothalamic proline-rich peptide and cobra venom Naja Naja Oxiana on dynamics of vestibular compensation following unilateral labyrinthectomy.

    abstract::We tested the action of proline-rich peptide (PRP-1) and cobra venom Naja Naja Oxiana (NOX) on Deiters' nucleus neurons at 3rd, 15th and 35th days after unilateral labyrinthectomy (UL). Early and late tetanic, post-tetanic potentiation and depression of Deiters'neurons to bilateral high frequency stimulation of hypoth...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Galoyan AA,Khalaji N,Hambardzumyan LE,Manukyan LP,Meliksetyan IB,Chavushyan VA,Sarkisian VH,Sarkissian JS

    更新日期:2010-11-01 00:00:00

  • The flavonoid glycosides, myricitrin, gossypin and naringin exert anxiolytic action in mice.

    abstract::The consumption of flavonoid-rich foods, in particular fruits and vegetables, has been epidemiologically associated with a reduced risk of heart disease, neurodegenerative disease, cancer and other chronic diseases. Flavonoid glycosides, the main class of flavonoids, have been shown to exert CNS-mediated activities, p...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Fernandez SP,Nguyen M,Yow TT,Chu C,Johnston GA,Hanrahan JR,Chebib M

    更新日期:2009-10-01 00:00:00

  • Metabolic Alterations in Developing Brain After Injury: Knowns and Unknowns.

    abstract::Brain development is a highly orchestrated complex process. The developing brain utilizes many substrates including glucose, ketone bodies, lactate, fatty acids and amino acids for energy, cell division and the biosynthesis of nucleotides, proteins and lipids. Metabolism is crucial to provide energy for all cellular p...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: McKenna MC,Scafidi S,Robertson CL

    更新日期:2015-12-01 00:00:00

  • Binding sites for L-glutamate in the central nervous system of the rat.

    abstract::The regional distribution of 2-amino-4-phosphonobutyrate (APB)/chloride-insensitive L-[3H]glutamate binding sites in the rat central nervous system was compared with that of APB/chloride-sensitive and with sodium-dependent binding sites. The distribution of APB-sensitive and APB-insensitive sites was not correlated, b...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Larder AP,McLennan H

    更新日期:1984-03-01 00:00:00

  • Studies on new, centrally active and reversible acetylcholinesterase inhibitors.

    abstract::We have synthesized the tertiary amines of pyridostigmine and neostigmine, 3-pyridinol dimethylcarbamate (norpyridostigmine) and 3-dimethylaminophenol dimethylcarbamate (norneostigmine) respectively, and we have tested their abilities to cross the blood-brain barrier and inhibit mouse brain AChE activity. The in vivo ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Arnal F,Coté LJ,Ginsburg S,Lawrence GD,Naini A,Sano M

    更新日期:1990-06-01 00:00:00

  • PPARγ and PGC-1α as therapeutic targets in Parkinson's.

    abstract::The peroxisome proliferator-activated receptor gamma (PPARγ) is a ligand-activated transcriptional factor that belongs to the nuclear hormone receptor superfamily. PPARγ was initially identified through its role in the regulation of glucose and lipid metabolism and cell differentiation. It also influences the expressi...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: Corona JC,Duchen MR

    更新日期:2015-02-01 00:00:00

  • Exploring the Therapeutic Potentials of Highly Selective Oxygenated Chalcone Based MAO-B Inhibitors in a Haloperidol-Induced Murine Model of Parkinson's Disease.

    abstract::Parkinson's disease (PD) is a neurodegenerative disorder of dopaminergic, noradrenergic, and serotonergic systems, in which dopamine, noradrenaline, and serotonin levels are depleted and lead to the development of motor and non-motor symptoms such as tremor, bradykinesia, weight changes, fatigue, depression, and visua...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Parambi DGT,Saleem U,Shah MA,Anwar F,Ahmad B,Manzar A,Itzaz A,Harilal S,Uddin MS,Kim H,Mathew B

    更新日期:2020-11-01 00:00:00

  • Percutaneous exposure to VX: clinical signs, effects on brain acetylcholine levels and EEG.

    abstract::The nerve agent VX has a variable and delayed absorption through the skin, which may have implications for treatment regimens. In the present study, central and peripheral effects of percutaneous VX intoxication were investigated in hairless guinea pigs. Although onset times of clinical signs varied considerably, the ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Joosen MJ,van der Schans MJ,van Helden HP

    更新日期:2008-02-01 00:00:00

  • Increased levels of methylated intermediates of phosphatidylcholine lead to enhanced phospholipase D activity.

    abstract::Previous work from this laboratory and others has shown that neurotransmitters can activate phospholipase D. Unlike the phospholipase C that specifically hydrolyzes inositol-containing phospholipids, phospholipase D in neuronal tissue specifically hydrolyzes phosphatidylcholine. One route for the synthesis of phosphat...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Jacobs TQ,Passarello B,Horwitz J

    更新日期:1998-08-01 00:00:00