NPD1 induction of retinal pigment epithelial cell survival involves PI3K/Akt phosphorylation signaling.

Abstract:

:Neuroprotectin D1 (NPD1), a docosahexaenoic acid (DHA)-derived lipid mediator, promotes survival in cells exposed to oxidative stress by inducing the activity of anti-inflammatory mediators and suppressing the expression of pro-inflammatory genes. Though retinal pigment epithelial (RPE) cells naturally produce NPD1 from DHA, investigating the mechanisms through which exogenous NPD1 induces cell survival is essential to assess mechanisms of actions and the potential of this lipid mediator for treatment of retinal degenerative diseases. The PI3K/Akt and mTOR/p70S6K pathways are responsible for supporting cell survival upon exposure to oxidative stress. In human ARPE-19 cells pretreated with NPD1 then exposed to varying concentrations of oxidative stress or repeated exposures to oxidative stress, Akt, mTOR, and p70S6K were phosphorylated to a greater extent and for a greater duration than cells not pretreated with NPD1. In addition to increased phosphorylation, a subsequent decreased rate of apoptosis was observed upon NPD1 treatment. Thus NPD1 bioactivity in RPE cells enhances activation of these pathways and promotes cell integrity and survival.

journal_name

Neurochem Res

journal_title

Neurochemical research

authors

Halapin NA,Bazan NG

doi

10.1007/s11064-010-0351-8

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

1944-7

issue

12

eissn

0364-3190

issn

1573-6903

journal_volume

35

pub_type

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