Abstract:
:Nerve growth factor (NGF) has been isolated from human placental tissue. Using the chicken embryo dorsal root ganglia assay, we determined levels of NGF activity for the amnion, placental cotyledons, cord serum, fetal serum, and maternal serum. The highest levels of NGF activity were measured in placental cotyledons. After homogenization and centrifugation of the placental cotyledons, the supernatant was sequentially chromatographed, at neutral pH, on Sephadex G-100, DEAE-11, and Sephadex G-150. A high-molecular-weight protein fraction (approximately 150,000), which contained all the biological activity, was isolated in this fashion. Analytical isoelectric focusing of this fraction revealed a basic protein component (pI 9.5) of the high-molecular-weight species. Assays for NGF activity of all protein components separated by analytical isoelectric focusing showed that NGF activity was associated only with the basic protein component. Correspondingly, preparative isoelectric focusing of the high-molecular-weight species yielded a basic protein with very high biological activity (1-3 ng per biological unit) that was immunochemically active against rabbit IgG made against mouse beta-NGF.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Goldstein LD,Reynolds CP,Perez-Polo JRdoi
10.1007/BF00964058subject
Has Abstractpub_date
1978-04-01 00:00:00pages
175-83issue
2eissn
0364-3190issn
1573-6903journal_volume
3pub_type
杂志文章abstract::Cyclin-dependent kinase 5 (cdk5) is a proline-directed serine/threonine kinase that is activated mostly by association with its activators, p35 and p39. Initially projected as a neuron-specific kinase, cdk5 is expressed ubiquitously and its kinase activity solely depends on the presence of its activators, which are al...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-008-9885-4
更新日期:2009-06-01 00:00:00
abstract::The finding that ketamine, an anaesthetic, can elicit a rapid antidepressant effect at low doses that lasts for weeks in patients with depression is arguably a major achievement in psychiatry in the last decades. However, the mechanisms of action are unclear. The glutamatergic hypothesis of ketamine action posits that...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-019-02744-1
更新日期:2020-01-01 00:00:00
abstract::We have developed a coupled Xenopus oocyte expression system for evaluating the functional effects of mutations in known or suspected adhesion molecules, which allows for a very rapid assessment of intercellular adhesion. As a model protein, we first used Protein zero (Po), an adhesion molecule that mediates self-adhe...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1010999622760
更新日期:2001-06-01 00:00:00
abstract::N-Methyl-D-aspartate (NMDA) and L-glutamate activate membrane receptors that produce substantial permeation of Na+, K+ and Ca2+ through the neuronal membrane. These ionic fluxes are intimately linked to processes that regulate neuronal survival, growth and differentiation. Intracellular free Ca2+ concentrations are th...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00966862
更新日期:1992-01-01 00:00:00
abstract::Glioblastoma is the most common and malignant brain cancer. In spite of surgical removal, radiation and chemotherapy, this cancer recurs within short time and median survival after diagnosis is less than a year. Glioblastoma stem cells (GSCs) left in the brain after surgery is thought to explain the inevitable recurre...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1868-2
更新日期:2016-07-01 00:00:00
abstract::The effects of pre-treatment with ENA-713, an acetylcholinesterase (AChE) inhibitor, on changes in pre- and postsynaptic cholinergic indices in gerbil brain following transient ischemia were studied at 4 and 14 days after recirculation. In the ischemic group, hippocampal acetylcholine (ACh) level was significantly red...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966804
更新日期:1994-02-01 00:00:00
abstract::Developing cellular models of sporadic Alzheimer's disease (sAD) is challenging due to the unknown initiator of disease onset and the slow disease progression that takes many years to develop in vivo. The use of human induced pluripotent stem cells (iPSCs) has revolutionised the opportunities to model AD pathology, in...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-018-2663-z
更新日期:2018-12-01 00:00:00
abstract::Treatment of neural membranes from rat cerebral cortex with phospholipase C (phosphatidylcholine cholinephosphohydrolase) inhibited the binding of radiolabelled antagonists to muscarinic acetylcholine receptors. This inhibition was incomplete, was not competitive, and did not appear to be related to the production of ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964517
更新日期:1984-05-01 00:00:00
abstract::Rat obturator nerve 16S acetylcholinesterase (16S AChE) was separated by sucrose gradient velocity sedimentation and compared to the 16S form of AChE similarly derived from endplate regions of anterior gracilis muscles. The 16S AChE from both tissues could only be extracted in high ionic strength buffer; as it aggrega...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965031
更新日期:1981-09-01 00:00:00
abstract::Astrocytes occupy a strategic position in the brain where they can act as an interface between neurones and blood vessels, and neurones and the cerebro-spinal fluid. This location is ideal for functioning as interoceptors, as they may sense changes in brain microenvironment and contribute to the adaptive homeostatic r...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-015-1562-9
更新日期:2015-12-01 00:00:00
abstract::In this study, we were interested to compare the responsiveness to growth factors, NGF, b-FGF and EGF and cytokines, IL1 beta, and TNF-alpha, in late passages (74-79) C6 glial cells committed astrocytes and astrocytes of advanced passages (26-28) in cultures derived from aged mouse cerebral hemispheres (MACH). Culture...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02527760
更新日期:1996-05-01 00:00:00
abstract::In membranes of rat striatum, phorbol 12-myristate 13-acetate (PMA), a potent activator of Ca2+/phospholipid-dependent protein kinase, enhanced adenylate cyclase activity by counteracting the inhibition elicited by GTP. Exposure to pertussis toxin caused a similar alteration of the GTP-regulation of the enzyme activit...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01101712
更新日期:1990-11-01 00:00:00
abstract::In our previous study vesamicol, an inhibitor of the acetylcholine transporter of the cholinergic vesicles, inhibited veratridine-evoked external Ca(2+)-dependent acetylcholine release from striatal slices but did not influence acetylcholine release observed in Ca(2+)-free medium (4). Here we examined if the effect of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968780
更新日期:1992-06-01 00:00:00
abstract::Umbilical cord blood-derived marrow stromal cells (UCB-MSCs) with high proliferation capacity and immunomodulatory properties are considered to be a good candidate for cell-based therapies. But until now, little work has been focused on the differentiation of UCB-MSCs. In this work, UCB-MSCs were demonstrated to be ne...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1186-x
更新日期:2013-12-01 00:00:00
abstract::There is a growing body of evidence confirming the involvement of oxidative stress and inflammation in pathogenesis of schizophrenia. Inter-individual variation in antioxidant capacity caused by different genetic profile could potentially influence patient's susceptibility to oxidative damage. In this study we evaluat...
journal_title:Neurochemical research
pub_type: 杂志文章,随机对照试验
doi:10.1007/s11064-012-0937-4
更新日期:2013-02-01 00:00:00
abstract::Evidence was accumulated indicating that cyclic nucleotides are involved in regulation of growth, differentiation and function of lymphoid cells. It was previously shown that the N-fragment (1-4) of thymosin beta 4 (Ac-Ser-Asp-Lys-Pro-OH) inhibits in vivo the entry of cell populations into S-phase. In the course of th...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00995153
更新日期:1995-01-01 00:00:00
abstract::Mutations in presenilin 1 (PS-1) are associated with most early-onset familiar Alzheimer's disease (AD). Previous studies have demonstrated that PS-1 mutations enhance the production of beta-amyloid (Aβ). In this study, we further examined the in vivo effects of PS-1 mutation on tau and synapse protein markers. The da...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0575-2
更新日期:2012-01-01 00:00:00
abstract::We investigated the ability of hydrogen peroxide (H2O2) to cause apoptotic cell death in cultured rat forebrain neurons and the potential mechanisms by which oxidative stress triggers delayed neuronal death. H2O2 (25 microM for 5 min) reduced cell viability to 34.5 +/- 8.3% of untreated controls 20 h after exposure, a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022403224901
更新日期:1997-03-01 00:00:00
abstract::Diisopropylfluorophosphate (DFP) is an irreversible inhibitor of acetylcholine esterase and a surrogate of the organophosphorus (OP) nerve agent sarin. The neurotoxicity of DFP was assessed as a reduction of population spike (PS) area elicited by synaptic stimulation in acute hippocampal slices. Two classical antidote...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1729-4
更新日期:2015-10-01 00:00:00
abstract::The effect of optic nerve transsection on proteolytic degradation of axonally transported proteins in the superior colliculus of the rabbit was studied. Proteolysis of labeled proteins was determined in vitro in small pieces of the superior colliculus. Within 2 hours after sectioning the optic nerve there was a decrea...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973280
更新日期:1988-07-01 00:00:00
abstract::miR-126 which is considered one of the most important miRNAs for maintaining vascular integrity, plays an important role in neuroprotection after cerebral ischemia-reperfusion (I-R). Moreover, vascular endothelial growth factor A (VEGFA), sprouty-related EVH1 domain-containing protein 1 (SPRED1), and Raf-1 are also in...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-02986-4
更新日期:2020-05-01 00:00:00
abstract::We examined the effects of small unilamellar vesicles composed of dipalmitoylphosphatidylcholine on rat cerebral cortical [3H]acetylcholine release. Synaptosomes from this region were loaded with the labeled transmitter, and then incubated with the lipid (0-6 mg/ml) for specified intervals before adding various secret...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970530
更新日期:1987-08-01 00:00:00
abstract::This study attempted to investigate the possible involvement of the brain stem noradrenergic system in the development of hypertension in spontaneously hypertensive rats. Steady-state norepinephrine, dopamine, serotonin and 5-hydroxyindoleacetic acid concentrations and norepinephrine turnover were determined in the in...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969761
更新日期:1989-01-01 00:00:00
abstract::The effect of oxidative stress, induced by Fe(2+)-EDTA system, on Na+,K(+)-ATPase, Na+/CA2+ exchanger and membrane fluidity of synaptosomes was investigated. Synaptosomes isolated from gerbil whole forebrain were incubated in the presence of 200 microM FeSO4-EDTA per mg of protein at 37 degrees C for 30 min. The oxida...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1021918931780
更新日期:1997-12-01 00:00:00
abstract::We propose the following scheme for cerebral uptake and overall metabolism of glucose in vivo: that brain selects from two pools of glucose anomers in arterial blood, that it takes up excess glucose, that glucose enters the brain tissue as glucose-6-phosphate through the actions of mutarotase and hexokinase, that some...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964568
更新日期:1985-02-01 00:00:00
abstract::We observed that AP-3, an antagonist of metabotropic glutamate receptors, reduced carbachol-induced hydrolysis of phospholipids in hippocampal slices. This inhibition could be explained in different ways, e.g.: 1) AP-3 acts also as antagonist of muscarinic receptors mediating the hydrolysis of phospholipids induced by...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022423415238
更新日期:1998-06-01 00:00:00
abstract::The effect of L-glutamate (Glu) and its structural analogs N-methyl-D-aspartate (NMDA), kainate (KA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA), on the activation of p42 mitogen activated protein kinase (MAPK) was examined in cultured chick radial glia cells, namely retinal Muller cells and cerebe...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027345808746
更新日期:1997-06-01 00:00:00
abstract::The aim of present study was to determine the effect of 3',4'-dihydroxyflavonol (DiOHF) on lipid peroxidation in experimental brain ischemia-reperfusion in rats. Present study was performed on the 34 male Wistar-albino rats, weigth 350-400 g. Experiment groups were designed as 1-Sham; 2-Ischemia-reperfusion; animal we...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1889-x
更新日期:2016-07-01 00:00:00
abstract::Blue light impinging on the many mitochondria associated with retinal ganglion cells (RGCs) in situ has the potential of eliciting necroptosis through an action on RIP1/RIP3 to stimulate RGC death in diseases like glaucoma and diabetic retinopathy. Cells in culture die when exposed to blue light. The death process is ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1946-5
更新日期:2016-09-01 00:00:00
abstract::Astrocytes transport the monocarboxylate acetate, but synaptosomes do not. The reason for this is unknown, because both preparations express monocarboxylate transporters (MCT). The transport and metabolism of lactate, another monocarboxylate, was examined in these two preparations, and the results were compared to tho...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000010450.21586.a6
更新日期:2004-01-01 00:00:00