Abstract:
:In this study, we were interested to compare the responsiveness to growth factors, NGF, b-FGF and EGF and cytokines, IL1 beta, and TNF-alpha, in late passages (74-79) C6 glial cells committed astrocytes and astrocytes of advanced passages (26-28) in cultures derived from aged mouse cerebral hemispheres (MACH). Cultures were grown in either DMEM or chemically defined medium (CDM/TIPS) in order to test the effects of growth factors or cytokines. The activity of glutamine synthetase (GS), a marker for astrocytes, was used as a test parameter. We found that treatment with growth factors increased GS activity in both glial cell culture systems with the exception of EGF in C-6 glial cells. Treatment with cytokines markedly decreased GS activity in the late passage C6 glial cells whereas only TNF-alpha had a similar effect on MACH astrocytes. In view of the generally opposite effects of growth factors and cytokines on GS activity, we speculate that these molecules which are also endogenously present in glial cells may play a role in the maintenance of cellular homeostasis.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Kazazoglou T,Fleischer-Lambropoulos E,Geladopoulos T,Kentroti S,Stefanis C,Vernadakis Adoi
10.1007/BF02527760subject
Has Abstractpub_date
1996-05-01 00:00:00pages
609-14issue
5eissn
0364-3190issn
1573-6903journal_volume
21pub_type
杂志文章abstract::Glutamate dehydrogenase (GDH) is a homohexameric enzyme that catalyzes the reversible oxidative deamination of L-glutamate to 2-oxoglutarate. Only in the animal kingdom is this enzyme heavily allosterically regulated by a wide array of metabolites. The major activators are ADP and leucine and inhibitors include GTP, p...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-013-1173-2
更新日期:2014-01-01 00:00:00
abstract::Consumption of fifteen percent alcohol, during gestation did not cause any decrease in the total calorie or fluid intake of the rats maintained on normal dietary regimen. However, the alcohol consumption by gestating mothers resulted in a decreased contents of both DNA and protein in the CNS of the in utero alcohol ex...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965614
更新日期:1989-11-01 00:00:00
abstract::Mutations in presenilin 1 (PS-1) are associated with most early-onset familiar Alzheimer's disease (AD). Previous studies have demonstrated that PS-1 mutations enhance the production of beta-amyloid (Aβ). In this study, we further examined the in vivo effects of PS-1 mutation on tau and synapse protein markers. The da...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0575-2
更新日期:2012-01-01 00:00:00
abstract::Exploring the neurochemical continuum between frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) with respect to monoamines and kynurenines in cerebrospinal fluid (CSF) and serum, may be useful to identify possible new research/therapeutic targets. Hence, we analysed monoamines and kynurenines in CS...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03002-5
更新日期:2020-05-01 00:00:00
abstract::In order to investigate the role of two free radical detoxificant enzymes in patients with aging brain disorders, superoxide dismutase (SOD) and catalase (CAT) activities have been measured in blood from male and female human patients of different ages with several types of aging brain disorders. When compared with ac...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02532336
更新日期:1996-08-01 00:00:00
abstract::The labeling of RNA in young and adult rat brain has been studied by measuring in vitro (tissue slices incubation) the incorporation of labeled uridine into RNA of total tissue and of the various subcellular fractions purified from cerebral hemispheres of 1- and 10-month-old rats. Gel electrophoretic analysis of the n...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965100
更新日期:1983-04-01 00:00:00
abstract::Ochratoxin A (OA) is a mycotoxin produced by several storage fungi, such as Aspergillus ochraceus and several Penicillium species. OA (3 mg/kg) was given intraperitoneally to pregnant mice on day 11 of gestation (day 1 = day of insemination), and neurochemical changes in brains of their offspring were examined at feta...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971631
更新日期:1988-12-01 00:00:00
abstract::Activation of glial cells has been observed in neurodegenerative diseases including Alzheimer's disease (AD). Aggregation of amyloid β (Aβ) is profusely observed as characteristic pathology in AD brain. In our previous study using microglial cell line BV-2, tissue-type transglutaminase (TG2) was found to be involved i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2284-y
更新日期:2018-01-01 00:00:00
abstract::Age related changes in brain cortex NO metabolism were investigated in mitochondria and cytosolic extracts from youth to adulthood. Decreases of 19%, 40% and 71% in NO production were observed in mitochondrial fractions from 3, 7, and 14 months old rats, respectively, as compared with 1-month-old rats. Decreased nNOS ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9570-z
更新日期:2008-07-01 00:00:00
abstract::CNS trauma has been associated with an increase in free radical production, but the cellular sources of this increase or the mechanism involved in the production of free radicals are not known. We, therefore, investigated the effects of trauma on free radical production in cultured neurons, astrocytes and BV-2 microgl...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1014875210852
更新日期:2002-02-01 00:00:00
abstract::In the experimental setting, taurine is known to be released from swollen cells to reestablish their normal volume. However, its clinical relevance has not been fully understood. This study was undertaken to reveal changes in cerebrospinal fluid (CSF) amino acids concentration in patients with severe traumatic brain i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-9693-4
更新日期:2005-01-01 00:00:00
abstract::Protein kinase C (PKC) plays a key role in cellular events including proliferation, survival and differentiation. Our previous study showed the effect of phorbol 12-myristate 13-acetate (PMA), a PKC activator, inducing a decrease in retinal cells proliferation. This effect was mediated by muscarinic type 1 receptors (...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9847-x
更新日期:2009-05-01 00:00:00
abstract::Ingestion of ammonium induces hyperammonemia which increases tubulin content in cerebrum but not in cerebellum. We have dissected 11 discrete areas of cerebrum and quantified the tubulin content in control and hyperammonemic rats. An heterogeneity in the induction of tubulin is shown. The areas more affected are ventr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965516
更新日期:1989-12-01 00:00:00
abstract::Somatostatin (SS) is an inhibitory regulator of secretory and proliferative responses that activates a group of receptors in the plasma membrane termed SSR1-5. SSR2 is one of the most abundant SSR, which also is expressed in high numbers in many neuroendocrine tumor types. Here, we describe a study of the presence and...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022848718109
更新日期:2003-04-01 00:00:00
abstract::The overlapping distribution of opioid and cholecystokinin (CCK) peptides and their receptors (mu and delta opioid receptors; CCK-A and CCK-B receptors) in the central nervous system have led to a large number of studies aimed at clarifying the functional relationships between these two neuropeptides. Most of the phar...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF02532381
更新日期:1996-11-01 00:00:00
abstract::Social isolation is one of the most potent stressors in the prepubertal period and may influence disease susceptibility or resilience in adulthood. The glucocorticoid response and, consequently, the adaptive response to stress involve important changes in mitochondrial functions and apoptotic signaling. Previous studi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0915-x
更新日期:2013-02-01 00:00:00
abstract::The enzymes degrading leucine enkephalin in human plasma and the inhibitors active on these enzymes were studied by kinetic and chromatographic techniques. Data obtained evidence the existence of complex kinetics of leu-enkephalin hydrolysis and of formation of its hydrolysis by-products. These appear to originate fro...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00995551
更新日期:1995-09-01 00:00:00
abstract::We have utilized quantitative autoradiography with the GABA(A) receptor chloride channel blocker [35S]t-butylbicyclophosphorothionate ([35S]TBPS) in rodent brain sections to investigate if differential proton modulation of various GABA(A) receptor subtypes expressed in various brain regions are differentially sensitiv...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000018849.54169.c4
更新日期:2004-04-01 00:00:00
abstract::Any spinal cord injury carries the potential for persistent disability affecting motor, sensory and autonomic functions. To prevent this outcome, it is highly desirable to block a chain of deleterious reactions developing in the spinal areas immediately around the primary lesion. Thus, early timing of pharmacological ...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-017-2459-6
更新日期:2019-01-01 00:00:00
abstract::Propofol and ketamine may provide certain degree of neuroprotection, but the underlying mechanism remains unclear to date. The cAMP response element-binding protein (CREB) was proposed that its phosphorylation at Ser133 (P-CREB) constituted a convergence point involved in neuroprotection. The purpose of this study was...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0582-3
更新日期:2012-01-01 00:00:00
abstract::Analysis in mouse brain slices of the uptake of acetyl-L-[N-methyl-14C]carnitine with time showed it to be concentrative, and kinetic analysis gave a Km of 1.92 mM and a Vmax of 1.96 mumol/min per ml, indicating the presence of a low-affinity carrier system. The uptake was energy-requiring and sodium-dependent, being ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964865
更新日期:1989-05-01 00:00:00
abstract::In this study, we observed the effects of metformin, one of the most widely prescribed drugs for the treatment of type 2 diabetes, on cell proliferation and neuroblast differentiation in the subgranular zone of the hippocampal dentate gyrus (SZDG) in Zucker diabetic fatty (ZDF) rats, which are a model for type 2 diabe...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-0115-5
更新日期:2010-04-01 00:00:00
abstract::Bilateral lesion of the nucleus basalis with ibotenic acid infusions in young and aged rats results in the degeneration of cholinergic neurons which innervate the cortex. As expected, high-affinity uptake of choline was decreased in the frontal cortex subsequent to the lesion. Twenty one days after surgery there was a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00974576
更新日期:1992-04-01 00:00:00
abstract::This study aimed at comparing the binding characteristics of [3H]ketanserin, a high-affinity serotonin 2A (5-HT2A) receptor antagonist, in the prefrontal cortex, hippocampus and striatum of human brain post-mortem. The results indicated the presence of a single population of binding sites in all the regions investigat...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027366413289
更新日期:1997-06-01 00:00:00
abstract::The antibodies against omega-conotoxin GVIA (omega-CTX GVIA; N-type voltage-dependent calcium channel [VDCC] blocker) and B1Nt (N-terminal segment [residues 1-13] of BI alpha1 subunits of VDCCs) were prepared, and the selectivity for each antigen omega-CTX GVIA and B1Nt was investigated. For the antigen selectivity of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1023208103438
更新日期:2003-06-01 00:00:00
abstract::Diisopropylfluorophosphate (DFP) is an irreversible inhibitor of acetylcholine esterase and a surrogate of the organophosphorus (OP) nerve agent sarin. The neurotoxicity of DFP was assessed as a reduction of population spike (PS) area elicited by synaptic stimulation in acute hippocampal slices. Two classical antidote...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1729-4
更新日期:2015-10-01 00:00:00
abstract::Brofaromine, a selective and reversible inhibitor of monoamine oxidase-A (MAO-A) was given to 19 women while 17 received placebo for 8 weeks. All met DSM III-R criteria for bulimia nervosa, a psychiatric disorder in which uncontrolled overeating episodes are accompanied by purging activities and extreme concerns about...
journal_title:Neurochemical research
pub_type: 临床试验,杂志文章
doi:10.1007/BF00975048
更新日期:1993-12-01 00:00:00
abstract::Using a model of middle cerebral artery occlusion (MCAO), we have previously demonstrated that treadmill exercise promotes angiogenesis in the ischemic penumbra through caveolin-1/VEGF signaling pathways. However, the function of caveolin-1/VEGF signaling in neurogenesis after MCAO has not been determined. In this stu...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2081-z
更新日期:2017-02-01 00:00:00
abstract::Acidosis (pH 6.0) led to significant decrease in high-affinity choline uptake by rat brain synaptosomes. The effects persisted following pH readjustment (7.4) of the incubation medium, consisting of decrease in both Km and Vmax of the affinity system. pH readjustment coincided with synaptosomal leakage of lactate dehy...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969699
更新日期:1995-07-01 00:00:00
abstract::Alzheimer's disease (AD) is pathologically characterized by presence of senile plaques in the hippocampus, which are composed mainly of extracellular deposition of a polypeptide known as the beta amyloid, the Aβ. It has been demonstrated on numerous occasions that it was the deposition and aggregation of this Aβ pepti...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0846-6
更新日期:2012-10-01 00:00:00