Abstract：
:Amphiphile dependency, solubility in aqueous solutions, and sensitivity to proteolysis of acetylcholinesterase (AChE) and nonspecific cholinesterase (nsChE) in the rat superior cervical ganglion were studied and compared to properties of soluble plasma cholinesterases. Ganglion AChE shows strong amphiphile dependency: an amphyphilic substance must be present in the homogenizing medium in order to obtain maximal apparent enzyme activity. Apparent activity of AChE solubilized in Ringer's solution was also increased after subsequent addition of a detergent. The 4 S molecular form, predominant in this extract (corresponding to the fastest electrophoretic band), is very sensitive to papain proteolysis but can be protected by a detergent. This molecular form therefore carries an important hydrophobic domain and is probably membrane bound in situ. The 10 S form of ganglionic AChE, extracted in Ringer's solution, is probably a soluble enzyme since, like soluble plasma enzymes, it is not amphiphile dependent and is rather resistant to proteolysis. Ganglion nsChE is more water soluble, less amphiphile dependent and more protease resistant than AChE.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Klinar B,Kamarić L,Sketelj J,Brzin Mdoi
10.1007/BF00964537subject
Has Abstractpub_date
1985-06-01 00:00:00pages
797-808issue
6eissn
0364-3190issn
1573-6903journal_volume
10pub_type
杂志文章abstract：:The spontaneously hypertensive rat (SHR) has been proposed as an animal model for attention-deficit hyperactivity disorder (ADHD). The behavioural problems have been suggested to be secondary to altered reinforcement mechanisms in which nucleus accumbens dopaminergic activity plays an important role. Interaction betwe...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973098
更新日期：1995-04-01 00:00:00
abstract：:Emerging data suggests that mitochondrial dysfunction is prominently involved in Alzheimer disease (AD) progression. Sirtuin-3 (Sirt3) is a member of the sirtuin family of nicotinamide adenine dinucleotide dependent deacetylases that regulates a variety of mitochondrial functions and suppresses mitochondria-related ph...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1630-1
更新日期：2015-08-01 00:00:00
abstract：:Repeated electroconvulsive shock is an effective treatment for affective disorders. Striatum, hippocampus and brainstem are involved in affective disorders. Sodium-potassium/ATPase is of paramount importance for the proper functioning of the brain and its involvement in the affective disorders has been claimed for a l...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9046-6
更新日期：2006-04-01 00:00:00
abstract：:Despite the consensus that activation of TWIK-related spinal cord K+ (TRESK) might contribute to the pathogenesis of chronic pain, the specific mechanisms underlying the transfer and development of pain signals still remain obscure. In the present study, we validated that TRESK was expressed in neurons instead of glia...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-2170-z
更新日期：2017-05-01 00:00:00
abstract：:The SAM strains, a group of related inbred strains consisting of senescence-prone inbred strains (SAMP) and senescence-resistant inbred strains (SAMR), have been successfully developed by selective inbreeding of the AKR/J strain of mice donated by the Jackson laboratory in 1968. The characteristic feature of aging com...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-009-9922-y
更新日期：2009-04-01 00:00:00
abstract：:Caspase activation and apoptotic events may take place in terminal regions far removed from the cell body and contribute to synapse loss in neurodegenerative diseases. For examination of events in terminals, we have developed a cell-free assay using quantitative flow cytometric analysis (fluorescence-activated cell so...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1019840417796
更新日期：2002-06-01 00:00:00
abstract：:We investigated the distribution of serotonin (5-HT) receptors of type 3 (5-HT3) in human brain areas, by means of the the specific binding of [3H]GR65630. The brains were obtained during autoptic sessions from 6 subjects. Human brain membranes and the binding of [3H]GR65630 were carried out according to standardized ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1010939530412
更新日期：2001-03-01 00:00:00
abstract：:This review summarizes the recent research development on mammalian sialidase molecular cloning. Sialic acid-containing compounds are involved in several physiological processes, and sialidases, as glycohydrolytic enzymes that remove sialic acid residues, play a pivotal role as well. Sialidases hydrolyze the nonreduci...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1020276000901
更新日期：2002-08-01 00:00:00
abstract：:Zinc oxide nanoparticles (ZnO NPs) have diverse utility these days ranging from being part of nanosensors to be ingredient of cosmetics. Present study was designed to report the effect of variable doses of ZnO NPs on selected aspects of male albino mice behavior. Nano particles were synthesized by sol-gel auto-combust...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-2090-y
更新日期：2017-02-01 00:00:00
abstract：:The goal of this study was to examine the responsiveness of an immortalized catecholaminergic neuronal line, 2N27, to various growth factors and identify those which promote catecholaminergic expression. 2N27 is a newly established neural cell line derived from fetal rat mesencephalic tissue and, thus, contains tyrosi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022482520358
更新日期：1997-03-01 00:00:00
abstract：:Radioactive azide derivatives of tetrodotoxin (TTX) were synthesized using 2-nitro-4-azidephenyl-[3H]beta alanine for the purpose of photolabeling of the Na channel. Three azide derivatives, N1, N2 and N3, were separated by ion exchange chromatography on Bio-Rex 70 resin and reversed phase high performance liquid chro...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965886
更新日期：1985-08-01 00:00:00
abstract：:The finding that ketamine, an anaesthetic, can elicit a rapid antidepressant effect at low doses that lasts for weeks in patients with depression is arguably a major achievement in psychiatry in the last decades. However, the mechanisms of action are unclear. The glutamatergic hypothesis of ketamine action posits that...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-019-02744-1
更新日期：2020-01-01 00:00:00
abstract：:Tau is a protein involved in regulation of microtubule stability, axonal differentiation and transport. Alteration of retrograde transport may lead to motor neuron degeneration. Thus alternative mRNA splicing and expression of tau isoforms were studied in a transgenic mouse model harboring the human SOD1 G93A mutation...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9057-3
更新日期：2006-05-01 00:00:00
abstract：:Exposure of rat pheochromocytoma PC12 cells to 0.1 mM 6-aminonicotinamide (6AN) for 24 hours resulted in a 500-fold increase in 6-phosphogluconate indicating active metabolism of glucose via the oxidative enzymes of the pentose phosphate pathway. Amounts of 6-phosphogluconate that accumulated in 6AN-treated cells at 2...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01000236
更新日期：1987-06-01 00:00:00
abstract：:Metformin is the most frequently used drug for the treatment of type 2 diabetes in humans. However, only little is known about effects of metformin on brain metabolism. To investigate potential metabolic consequences of an exposure of brain cells to metformin, we incubated rat astrocyte-rich primary cultures with this...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1733-8
更新日期：2017-01-01 00:00:00
abstract：:Bovine brain synaptic membranes which were frozen and then extensively washed showed low affinity [3H]muscimol binding. These membranes contained GABA and calmodulin, apparently tightly bound within the membrane fraction. Membranes which were additionally treated with the detergent Triton X-100 showed high affinity [3...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00970756
更新日期：1988-09-01 00:00:00
abstract：:Several lines of evidence indicate that ATP may play an important role in Long-Term Potentiation. In this investigation we evaluated the effect of a memory task (step-down inhibitory avoidance) on the synaptosomal ecto-enzymes (ATP diphosphohydrolase and 5'-nucleotidase) involved in the degradation of ATP to adenosine...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021084422228
更新日期：1998-07-01 00:00:00
abstract：:Antidepressive drugs offer considerable symptomatic relief in mood disorders and, although commonly discovered by screening with single biological targets, most interact with multiple receptors and signaling pathways. Antidepressants require a treatment regimen of several weeks before clinical efficacy is achieved in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-8513-9
更新日期：2005-11-01 00:00:00
abstract：:Hyperprolinemia type II (HPII) is an autosomal recessive disorder caused by the severe deficiency of enzyme delta1-pyrroline-5-carboxylic acid dehydrogenase leading to tissue accumulation of proline. Chronic administration of Pro led to significant reduction of cytosolic ALT activity of olfactory lobes (50.57%), cereb...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-7026-2
更新日期：2004-12-01 00:00:00
abstract：:Neuronal differentiation and aging are known to involve many genes, which may also be differentially expressed during these developmental processes. From primary cultured cerebral cortical neurons, we have previously identified various differentially expressed gene transcripts from cultured cortical neurons using the ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-8800-5
更新日期：2005-10-01 00:00:00
abstract：:The present study has attempted to elucidate the alteration of serotonin turnover after 24 h REM sleep deprivation in different regions in brain of young rat. Sleep deprivation was induced by the inverted flower pot technique. Results of this study show increased serotonin turnover after 24 h REM sleep deprivation in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-9139-7
更新日期：2006-01-01 00:00:00
abstract：:Two models of perturbed cerebellar ontogenesis were obtained by a single administration of methylazoxymethanol (MAM), a potent antimitotic agent, to mouse pups either on the day of birth (MAM0 mice) or at postnatal day 5 (MAM5 mice). The alterations of the cerebellar GABAergic system were studied by measuring glutamic...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968010
更新日期：1992-07-01 00:00:00
abstract：:More than a decade has passed since Zic family zinc finger proteins were discovered to be transcription factors controlling neuroectodermal differentiation (neural induction) in Xenopus laevis embryos. Although BMP-signal blocking has been shown to be a major upregulator of Zic genes in neuroectodermal differentiation...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-011-0422-5
更新日期：2011-07-01 00:00:00
abstract：:Mutations in presenilin 1 (PS-1) are associated with most early-onset familiar Alzheimer's disease (AD). Previous studies have demonstrated that PS-1 mutations enhance the production of beta-amyloid (Aβ). In this study, we further examined the in vivo effects of PS-1 mutation on tau and synapse protein markers. The da...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0575-2
更新日期：2012-01-01 00:00:00
abstract：:The effects of a number of drugs that deplete catecholamines or block their receptors were investigated on the levels of free amino acids and glutamate decarboxylase activity in rat striatum and substantia nigra, in an attempt to clarify the possible links between catecholamine and amino acid transmitter function. Whi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966009
更新日期：1977-10-01 00:00:00
abstract：:Alterations in lysosomal proteases have been implicated in many neurodegenerative diseases. The current study demonstrates a concentration-dependent decrease in PC12 cell viability and transient changes in cystatin C (CYSC), cathepsin B (CATB), cathepsin D (CATD) and caspase-3 following exposure to H2O2. Furthermore, ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9338-5
更新日期：2007-09-01 00:00:00
abstract：:Convection enhanced delivery of 6-hydroxydopamine (6-OHDA) to the rat striatum results in a model of Parkinson's disease. An important feature of this unilateral model is the progressive loss of dopaminergic (DA) neurons over the course of several weeks. To improve the understanding of this model, gene expression chan...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0133-3
更新日期：2010-05-01 00:00:00
abstract：:Populations of enriched glial precursor cells and astrocytes isolated from primary cultures of newborn rat brain were used to study the synthesis of sulfated glycoproteins. Both cell types incorporated [3H]glucosamine and [35S]sulfate into carbohydrate side chains of proteoglycans and glycoproteins. The rate of incorp...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965947
更新日期：1986-08-01 00:00:00
abstract：:A panel of mouse monoclonal antibodies to rat and human myelin-associated glycoprotein (MAG) was developed. Normal mice were unresponsive to rat MAG, and successful immunization with rat MAG was only achieved in autoimmune NZB mice. By contrast, all strains of mice were responsive to human MAG. The monoclonal antibodi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964654
更新日期：1985-04-01 00:00:00
abstract：:Protocatechuic acid (PA), a major metabolite of anthocyanins, has been reported to possess antioxidant and anti-inflammatory activities. However, the effects of PA on LPS-induced inflammatory responses in microglia have not been reported. The aim of this study was to investigate the anti-inflammatory effects and molec...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1646-6
更新日期：2015-08-01 00:00:00
