Abstract：
:Caspase activation and apoptotic events may take place in terminal regions far removed from the cell body and contribute to synapse loss in neurodegenerative diseases. For examination of events in terminals, we have developed a cell-free assay using quantitative flow cytometric analysis (fluorescence-activated cell sorting) of neuronal particles in a P2 synaptosomal preparation (P-2) from rat brain as a model system. Staurosporine-induced loss of neuronal particles was blocked by nonselective caspase inhibition (z-VAD-fmk) and by calpain inhibition (calpain inhibitor II [ALLM]). Phosphatidylserine exposure was increased in the P-2 by staurosporine treatment, and this increase was blocked by a peptide inhibitor of caspase-3-like activity (Ac-DEVD-CHO). Increased caspase activity in the crude synaptosomal fraction was confirmed by direct measurement with a fluorometric assay. These results indicate activation of both caspase and calpain in the P-2 fraction and suggest a role for these cysteine proteases in the in vitro degradation of nerve terminals.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Gylys KH,Fein JA,Cole GMdoi
10.1023/a:1019840417796keywords:
subject
Has Abstractpub_date
2002-06-01 00:00:00pages
465-72issue
6eissn
0364-3190issn
1573-6903journal_volume
27pub_type
杂志文章abstract：:A study was undertaken in rats to evaluate the effects of short-term oral ethanol administration on the levels of fatty acid ethyl esters (FAEE) in brain and peripheral organs in the presence and absence of pretreatment with L-carnitine. Administration of ethanol to rats for seven days resulted in fatty acid ethyl est...
journal_title：Neurochemical research
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pub_type： 杂志文章,撤回出版物
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更新日期：2009-12-01 00:00:00
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更新日期：2013-02-01 00:00:00
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