Abstract：
:Bovine brain synaptic membranes which were frozen and then extensively washed showed low affinity [3H]muscimol binding. These membranes contained GABA and calmodulin, apparently tightly bound within the membrane fraction. Membranes which were additionally treated with the detergent Triton X-100 showed high affinity [3H]muscimol binding. These membranes did not appear to contain GABA or calmodulin. Transmission electron microscopy studies demonstrated that the washed membrane fraction contained many synaptosomal and vesicular structures. Triton treatment led to the extensive rupture of these structures. These studies explain the well-reported findings of tightly bound GABA and calmodulin in brain membrane fractions, as being due to the entrapment of these compounds inside sealed membrane-bound structures which are still present after a freeze-thaw and extensive wash treatment, their complete removal requiring Triton-treatment to rupture the vesicles.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
O'Neill C,Williams DCdoi
10.1007/BF00970756subject
Has Abstractpub_date
1988-09-01 00:00:00pages
871-7issue
9eissn
0364-3190issn
1573-6903journal_volume
13pub_type
杂志文章abstract：:The incubation of neuronal nitric oxide synthase with the five amyloid peptide fragments [Aβ17-21; Aβ25-29; Aβ29-33; Aβ33-37; Aβ25-37] catalyzed the formation of fibrils. The role of neuronal isomer (nNOS) involved the entrapment of free monomers and seed aggregates to initiate the events of nucleation and elongation,...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1206-x
更新日期：2014-01-01 00:00:00
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journal_title：Neurochemical research
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更新日期：2008-07-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964358
更新日期：1978-02-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：1996-09-01 00:00:00
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更新日期：1989-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11064-015-1662-6
更新日期：2015-09-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：2017-02-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：1995-07-01 00:00:00
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更新日期：2020-01-01 00:00:00
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journal_title：Neurochemical research
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doi：10.1023/a:1021623700009
更新日期：2002-11-01 00:00:00
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更新日期：1990-08-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022023207326
更新日期：1997-11-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2505-z
更新日期：2018-05-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966022
更新日期：1977-02-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2477-z
更新日期：2018-03-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：2010-08-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964651
更新日期：1985-04-01 00:00:00
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更新日期：2010-03-01 00:00:00
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journal_title：Neurochemical research
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更新日期：1992-08-01 00:00:00
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更新日期：2008-09-01 00:00:00
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更新日期：2015-03-01 00:00:00
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journal_title：Neurochemical research
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doi：10.1007/s11064-020-03202-z
更新日期：2021-01-03 00:00:00
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journal_title：Neurochemical research
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更新日期：1994-02-01 00:00:00
abstract：:The neurotrophins brain derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) are both expressed in developing cerebellum in addition to their tyrosine kinase receptors. TrkB and TrkC. In contrast to BDNF.NT-3 has only a negligible or a transient survival activity on cultured cerebellar granule neurons. The gra...
journal_title：Neurochemical research
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更新日期：1996-07-01 00:00:00
abstract：:Abnormally high levels of iron are observed in the brain of patients suffering from neurodegenerative disorders. The mechanisms involved in iron accumulation in neurodegenerative disorders remain poorly understood. In the present study we investigated the effects of aging and neonatal iron overload on the mRNA express...
journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：2010-04-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common type of neurodegenerative dementia that affects the elderly population. Nerve growth factor (NGF) contributes to the survival, regeneration and death of neurons during aging and in neurodegenerative diseases. Recently, research has shown that NGF is related to the pathology,...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
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更新日期：2016-06-01 00:00:00
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journal_title：Neurochemical research
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doi：10.1023/b:nere.0000023600.25994.11
更新日期：2004-06-01 00:00:00
abstract：:Metabolic turnover of axonally transported glycoproteins has been examined in membranous and soluble subfractions of goldfish optic tectum following intraocular injection of [3H]fucose. Radioactivity in total transported glycoproteins reached a maximum in the tectum after 24-30 hr, then declined with a half-life of ap...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964224
更新日期：1980-04-01 00:00:00
abstract：:The monoclonal antibody 14E immunocytochemically stains the nuclear membrane of oligodendrocytes but not myelin in tissue sections of adult normal human white matter. The nuclear membranes of Schwann cells in human peripheral nerve and cerebellar Bergmann glia were also visualized with this antibody. In actively demye...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00993270
更新日期：1992-09-01 00:00:00
