Abstract：
:Glutamine synthetase (GS) from the chick brain was purified to apparent homogeneity by ammonium sulfate fractionation followed by affinity chromatography, electrofocusing and Sephadex G-150 chromatography. The purified enzyme showed a single band on sodium dodecyl sulfate analysis in polyacrylamide gel. By sedimentation equilibrium analysis and gel electrophoresis analysis, it was shown that the enzyme has a subunit molecular weight of 45,000 and a native molecular weight of 364,000, which is consistent with an octameric structure. Sedimentation analysis in the presence of Mg2+ revealed three different forms of macromolecules corresponding respectively to a monomer, a tetramer and an octamer. Among eight cations tested (Ca2+, Co2+, Fe2+, Li+, Mg2+, Mn2+, Ni2+, Zn2+) only Co2+, Mg2+ and Mn2+ supported GS activity; the order of activatory ability was Mg2+ greater than Co2+ greater than Mn2+. The maximum activating effect of Mn2+ occurs only within a very narrow range of concentration: with an excess of cation causing strong inhibition of GS activity. For each cation, maximal GS activity occurs at a defined cation/ATP ratio. A regulatory system in which Mn2+, modulates the Mg2+ dependent GS activity, is proposed; such cation interactions may be of significance in the intracellular control of glutamine synthesis.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Tholey G,Bloch S,Ledig M,Mandel P,Wedler Fdoi
10.1007/BF00970934subject
Has Abstractpub_date
1987-11-01 00:00:00pages
1041-7issue
11eissn
0364-3190issn
1573-6903journal_volume
12pub_type
杂志文章abstract：:This study evaluates whether physiological variables differentially affect the local synthesis of protein constituents of synapses in subcellular fractions containing pinched-off dendrites (synaptodendrosomes). Synaptodendrosomes were pulse-labeled in a medium containing 35S-methionine with 3 or 25 mM KCl and in the p...
journal_title：Neurochemical research
pub_type： 杂志文章
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：1989-11-01 00:00:00
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更新日期：1989-12-01 00:00:00
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更新日期：1995-09-01 00:00:00
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更新日期：2009-04-01 00:00:00
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doi：10.1023/a:1021916509858
更新日期：1997-10-01 00:00:00
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更新日期：1999-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：1991-11-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：1986-06-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965151
更新日期：1994-09-01 00:00:00
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更新日期：2015-11-01 00:00:00
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更新日期：2017-11-01 00:00:00
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更新日期：1980-02-01 00:00:00
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更新日期：2004-01-01 00:00:00
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更新日期：2002-08-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2012-12-01 00:00:00
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更新日期：2007-12-01 00:00:00
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更新日期：2003-04-01 00:00:00
abstract：:The folate cycle, which has a close correlation with nucleic acid synthesis as well as with sulfur amino acid metabolism, may have some bearing on the pathogenesis of amyotrophic lateral sclerosis. The folate cycle hypothesis offers a unified explanation of the reduction of RNA and the elevation of taurine in nervous ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963985
更新日期：1984-03-01 00:00:00
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journal_title：Neurochemical research
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更新日期：1988-05-01 00:00:00
abstract：:Epilepsy is a disorder of the brain characterized by an enduring predisposition to generate epileptic seizures. The glycolytic inhibitor 2-deoxy-D-glucose (2-DG) has been reported to exert antiepileptic effects by upregulating KATP subunits (kir6.1 and kir6.2). We evaluated whether 2-DG exhibits anti-seizure effect by...
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更新日期：2019-04-01 00:00:00
abstract：:The kinetics and specificity of taurine and beta-alanine uptake were studied in primary cultures of rat astrocytes under identical experimental conditions. The uptake consisted of nonsaturable penetration and saturable high-affinity transport that was strictly sodium dependent. The cells accumulated taurine more effec...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00967969
更新日期：1986-02-01 00:00:00
abstract：:The effect of isoproterenol, norepinephrine, dopamine, and serotonin on the accumulation of adenosine 3',5'-monophosphate (cAMP) was studied in homogeneous neuronal cultures from 8-day chick embryo hemispheres. Among the catecholamines, isoproterenol had a more pronounced effect on the accumulation of cAMP. Norepineph...
journal_title：Neurochemical research
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更新日期：1980-10-01 00:00:00
