Abstract:
:Microdissection of selected regions of central nervous system (CNS) has provided the basis of modern chemoarchitectonics. Laser microdissection is a modern variant of the "Palkovits punch" technique and used together with gene array analysis has revolutionalized CNS molecular analysis. Here we describe the use of such an approach to elucidate molecules selectively expressed in magnocellular neuroendocrine cells (MCNs) in the supraoptic nucleus (SON). We found 123 genes that are preferentially expressed in the SON, and of these, 89 were substantially osmoregulated in their expression. One of these, C1q domain containing 1, is a novel gene that is osmoregulated much more than even vasopressin itself.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Mutsuga N,Gainer Hdoi
10.1007/s11064-005-9008-4keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
189-99issue
2eissn
0364-3190issn
1573-6903journal_volume
31pub_type
杂志文章abstract::Recent evidence has linked striatal amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor function to the adverse effects of long-term dopaminergic treatment in Parkinson's disease. The phosphorylation of AMPA subunit, GluR1, reflects AMPA receptor activity. To determine whether serine phosphorylation of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9177-9
更新日期:2006-11-01 00:00:00
abstract::Several lines of evidence suggest that the mechanism underlying drug-induced neuronal apoptosis is initiated by the increased production of reactive oxygen species (ROS). 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), a neurotoxin, has been shown to initiate an apoptotic cascade by increasing ROS in the dopamine...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1208-8
更新日期:2014-02-01 00:00:00
abstract::Ingestion of ammonium induces hyperammonemia which increases tubulin content in cerebrum but not in cerebellum. We have dissected 11 discrete areas of cerebrum and quantified the tubulin content in control and hyperammonemic rats. An heterogeneity in the induction of tubulin is shown. The areas more affected are ventr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965516
更新日期:1989-12-01 00:00:00
abstract::Nitric oxide (NO) regulates numerous physiological process and is the main source of reactive nitrogen species (RNS). NO promotes cell survival, but it also induces apoptotic death having been involved in the pathogenesis of several neurodegenerative diseases. NO and superoxide anion react to form peroxynitrite, which...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1805-9
更新日期:2016-02-01 00:00:00
abstract::Members of the PDGF family have multiple roles during embryogenesis and in a variety of pathological situations in the adult. One of the major sites of PDGF-B expression in adult mammals are postmitotic CNS neurons. Combined with reported neurotrophic and neuroprotective effects of exogenously administered PDGFs, this...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022421001288
更新日期:2003-02-01 00:00:00
abstract::The objective of the present study was to investigate the effects of preincubation of hippocampus homogenates in the presence of homocysteine or methionine on Na+, K+-ATPase and Mg2+-ATPase activities in synaptic membranes of rats. Homocysteine significantly inhibited Na+, K+-ATPase activity, whereas methionine had no...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1013907104585
更新日期:2001-11-01 00:00:00
abstract::A variety of naturally occurring amino acids, their isomers, and synthetic analogs were tested for their ability to inhibit uptake of [3H]glutamate into presynaptic vesicles from bovine cerebral cortex. Strongest inhibition (Ki < 1mM) was observed for trans-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) and erythr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966925
更新日期:1993-01-01 00:00:00
abstract::The current study demonstrates that in hippocampal neurons mitochondrial Ca(2+) processing supports Ca(2+) influx via ionotropic glutamate (Glu) receptors. We define mitochondrial Ca(2+) processing as Ca(2+) uptake via mitochondrial Ca(2+) uniporter (MCU) combined with subsequent Ca(2+) release via mitochondrial Na(+)...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1819-3
更新日期:2016-06-01 00:00:00
abstract::Passive avoidance (PA) conditioning is a fear motivated task able to initiate a cascade of altered gene expression within the hippocampus, a structure critical to learning and memory. We have previously shown that neurofibromin (NF1) and amyloid precursor protein (APP), two genes implicated in cognitive function, are ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0949-0
更新日期:2013-03-01 00:00:00
abstract::Factors secreted by C6 glioma cells which induce electrical resistances across endothelial monolayers in an in vitro blood-brain barrier model have been partially characterised for the first time. These transendothelial electrical resistances (TEERs) were only evident when cell-free conditioned medium derived from C6 ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020932121378
更新日期:1998-12-01 00:00:00
abstract::The participation of voltage-sensitive Na+ channels (VSSC) on the changes on internal (i) Na+, K+, Ca2+, and on DA, Glu, and GABA release caused by different concentrations of 4-AP was investigated in striatum synaptosomes. TTX, which abolished the increase in Na(i) (as determined with SBFI), induced by 0.1 mM 4-AP on...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000013737.17288.ce
更新日期:2004-02-01 00:00:00
abstract::Cathepsin B (EC 3.4.22.1) was purified 746-fold with a 21% recovery from bovine brain by autolysis, fractional precipitation with acetone, carboxy-methyl-Sephadex chromatography, affinity chromatography on a cystamine containing column and gel filtration chromatography. The purified cathepsin B eluted on gel filtratio...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965209
更新日期:1986-06-01 00:00:00
abstract::The incorporation of [gamma-32P]ATP into proteins of rat brain polyribosomes was studied in vitro. The effects of cyclic nucleotides, calcium, hemin, ACTH, GTP, and spermine were examined. The incorporation of phosphate into proteins increased with time and phosphatase activity was very low; thus, the extent of phosph...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973039
更新日期:1984-09-01 00:00:00
abstract::Nicotinic agonists stimulate 22Na flux in rat pheochromocytoma PC12 cells. The stimulatory effect of carbamylcholine is maximal at 1 mM, while the stimulatory effect of nicotine and anatoxin maximize at the same level at 100 microM and 10 microM, respectively. The tertiary amines arecolone and isoarecolone have no eff...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965571
更新日期:1991-04-01 00:00:00
abstract::Donepezil is a selective inhibitor of acetylcholinesterase (AChE) clinically used for treating Alzheimer's disease. Cholinergic effects after short-term exposure of donepezil (up to 12 h) have been extensively studied in rats, but few have addressed the potential long-term effects. After 14 days administration (1x3 mg...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-8828-6
更新日期:2005-12-01 00:00:00
abstract::(4S)-4-Amino-5,6-heptadienoic acid [S)-gamma-allenyl-GABA; MDL 72483) is a potent inactivator of brain GABA-T in mice; (ED50 (i.p.) = 60 mg.kg-1; ED50 (oral) = 70 mg.kg-1). Its anticonvulsant effects against 3-mercaptopropionic acid (MPA)-induced seizures in mice is related to the elevation of whole brain GABA concent...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966092
更新日期:1991-03-01 00:00:00
abstract::During pregnancy, emergence of endogenous opioid inhibition of oxytocin neurons is revealed by increased oxytocin secretion after administration of the opioid receptor antagonist, naloxone. Here we show that prolonged estradiol-17beta and progesterone treatment (mimicking pregnancy levels) potentiates naloxone-induced...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9506-7
更新日期:2008-04-01 00:00:00
abstract::DNase I sensitivity experiments were performed utilizing DNA probes to genes which are either transcribed in rat cortical neurons (the 68 kDa neurofilament gene and the neuron-specific enolase gene) or are transcriptionally silent (albumin). Results suggest that unlike liver, in which a hierarchy in chromatin conforma...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969627
更新日期:1989-02-01 00:00:00
abstract::Parkinson's disease (PD) is a neurodegenerative disease, with genetics and environment contributing to the disease onset. The limited pathological cognize of the disease restrained the approaches to improve the clinical treatment. Recently, studies showed that endoplasmic reticulum (ER) stress played an important role...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1922-0
更新日期:2016-08-01 00:00:00
abstract::Cortical spreading depression (CSD) enhances ischemic tolerance to temporary focal ischemia. Although this effect most likely requires the expression or activation of neuroprotective factors, their identity remains relatively unknown. One important factor involved in neuroprotection is adenosine monophosphate-dependen...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1447-3
更新日期:2014-12-01 00:00:00
abstract::Fecal incontinence and constipation still remain the major complications after procedures for anorectal malformations (ARMs). Previous studies have demonstrated a decrease of neural cell in lumbosacral spinal cord of ARMs patients and rat models. However, the underlying mechanism remains elusive. In this study, the ne...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2354-1
更新日期:2017-11-01 00:00:00
abstract::The monoclonal antibody 14E immunocytochemically stains the nuclear membrane of oligodendrocytes but not myelin in tissue sections of adult normal human white matter. The nuclear membranes of Schwann cells in human peripheral nerve and cerebellar Bergmann glia were also visualized with this antibody. In actively demye...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00993270
更新日期:1992-09-01 00:00:00
abstract::The control and maintenance of the intracellular redox state is an essential task for cells and organisms. NAD(+) and NADH constitute a redox pair crucially involved in cellular metabolism as a cofactor for many dehydrogenases. In addition, NAD(+) is used as a substrate independent of its redox-carrier function by enz...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-012-0760-y
更新日期:2012-11-01 00:00:00
abstract::Silibinin has been widely used to treat liver diseases due to its antioxidant activity. However, the effects of silibinin on the central nervous system have not been thoroughly investigated. The pathological hallmarks of Alzheimer's disease are the accumulation of amyloid β protein, development of neurofibrillary tang...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2389-3
更新日期:2017-12-01 00:00:00
abstract::Tetrahydrobiopterin and the folate coenzymes can reciprocally interact in ways that would be useful to the metabolic pathways subserved by both of these coenzymes. Thus, through one of the reactions catalyzed by methylene tetrahydrofolate reductase, 5-CH3-H4-folate can regenerate BH4 from q-BH2 and q-BH2 can provide a...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00965847
更新日期:1991-09-01 00:00:00
abstract::A major part of the Ca-activated proteolytic activity in the soluble fraction from rabbit brain could be due to the activity of the neutral thiol-proteases calpain I and II. The activity of calpains exceeded that of the endogenous inhibitor, calpastatin, at all developmental stages studied. The level of calpains incre...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965622
更新日期:1989-11-01 00:00:00
abstract::The MAO inhibitor phenelzine (PLZ) at a dose of 25 mg/kg does not affect the behavior of rats. In contrast, the equivalent dose of a deuterated analog (alpha, alpha, beta, beta-tetradeutero-PLZ, d4PLZ) elicits a biphasic behavioral syndrome in rats. In an attempt to correlate changes in cerebral monoamines with behavi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973322
更新日期:1988-02-01 00:00:00
abstract::Pyridostigmine bromide (PB), an inhibitor of acetylcholinesterase, has been used as a prophylactic for nerve gas poisoning. N,N'-diethyl-m-toluamide (DEET) is the active ingredient in most insect repellents and is thought to interact synergistically with PB. Since PB can inhibit the binding of organophosphates to tubu...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1007527129743
更新日期:2000-01-01 00:00:00
abstract::Attempts were made to solubilize acetylcholinesterase (AChE) from microsomal membranes isolated from rabbit white muscle. The preparative procedure included a step in which the microsomes were incubated in a solution containing high salt concentration (0.6 M KCl). About 15% of the total enzyme activity could be solubi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971007
更新日期:1987-07-01 00:00:00
abstract::We have shown that acute ammonia toxicity is mediated by activation of the NMDA type of glutamate receptors. Although it is well known that L-carnitine prevents acute ammonia toxicity, the underlying molecular mechanism is not clear. We suspected that L-carnitine would prevent ammonia toxicity by preventing the toxic ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971588
更新日期:1994-03-01 00:00:00