Abstract:
:Factors secreted by C6 glioma cells which induce electrical resistances across endothelial monolayers in an in vitro blood-brain barrier model have been partially characterised for the first time. These transendothelial electrical resistances (TEERs) were only evident when cell-free conditioned medium derived from C6 glioma cells was applied to the basolateral surfaces of confluent ECV304 or ECV304-9 cells which are both human umbilical vein endothelial cell lines (HUVEC). Electrical resistance values as high as 600 ohm. sq cm were obtained with this blood-brain barrier model and ultrafiltration techniques suggest that any factor(s) in the conditioned medium responsible for these TEERs have molecular masses of less than 1000 Da. Enzymic proteolysis and heat treatment carried out on the conditioned medium failed to inhibit its effect on the HUVEC monolayers suggesting that these C6 cell-secreted factors are unlikely to be proteins.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Ramsohoye PV,Fritz IBdoi
10.1023/a:1020932121378subject
Has Abstractpub_date
1998-12-01 00:00:00pages
1545-51issue
12eissn
0364-3190issn
1573-6903journal_volume
23pub_type
杂志文章abstract::Metabotropic glutamate receptors (mGluR) play a role in synaptic transmission, neuronal modulation and plasticity but their action in epileptic activity is still controversial. On the other hand adenosine acts as a neuromodulator with endogenous anticonvulsive properties. Since cerebellum from epileptic patients has s...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9275-8
更新日期:2007-07-01 00:00:00
abstract::Ménière's disease (MD) is characterized by the triad of fluctuating hearing loss, episodic vertigo and tinnitus, and by endolymphatic hydrops found on post-mortem examination. Increasing evidence suggests that oxidative stress is involved in the development of endolymphatic hydrops and that cellular damage and apoptot...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0304-2
更新日期:2010-12-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2016-06-01 00:00:00
abstract::Mice infected with the Venezuelan equine encephalomyelitis virus showed a significant decrease in the GABA content of cerebral hemispheres. Activity of the enzyme which synthetizes GABA, glutamate decarboxylase, is also reduced in whole cerebral hemispheres, neostriatum, and frontal cortex of infected animals, as comp...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964333
更新日期:1980-02-01 00:00:00
abstract::Parkinson's disease (PD) is characterized by the presence of insoluble protein clusters containing α-synuclein. Impairment of mitochondria, endoplasmic reticulum, autophagy and intracellular trafficking proper function has been suggested to be caused by α-synuclein toxicity, which is also associated with the higher le...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
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更新日期:2018-12-01 00:00:00
abstract::The present study examined the potential membrane retention of desipramine (DMI) following exposures of 293-hNET cells to DMI, and its effect on [3H]NE uptake. Incubation of cells with 500 nM DMI for 1 h or 1 day persistently inhibited the uptake of [3H]NE up to 7 days, despite daily repeated washing of cells with dru...
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更新日期:2004-02-01 00:00:00
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journal_title:Neurochemical research
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更新日期:2003-06-01 00:00:00
abstract::An efficient exchange of lactate between different cell types (such as astrocytes and neurones) would require that lactate transporters are expressed in contiguous parts of the respective plasma membranes. To settle this issue we explored the subcellular expression pattern of monocarboxylate transporters (MCTs) by use...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
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更新日期:2002-02-01 00:00:00
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journal_title:Neurochemical research
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更新日期:2017-02-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章,评审
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更新日期:2015-02-01 00:00:00
abstract::Single prolonged stress (SPS) rats is a rodent model of post traumatic stress disorder (PTSD). Abnormal hippocampal morphology and function were found in the PTSD patients. Our previous study has shown that SPS induce loss of hippocampal neurons. But the effects of SPS on glial cells in the hippocampus have not been e...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1549-6
更新日期:2015-05-01 00:00:00
abstract::Association of the candidate gene DLST with late-onset Alzheimer's disease (LOAD) risk has been suggested on the basis of case-control studies. This gene, located on chromosome 14q24.3, encodes a subunit of a mitochondrial component known to be defective in AD, the alpha-ketoglutarate dehydrogenase complex. Positive r...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000014833.54481.1d
更新日期:2004-03-01 00:00:00
abstract::The insoluble fraction of ox-brain, which had previously been shown to have a non-linear affinity for Na+ and K+, was prepared. Acetylcholine (1 x 10(-8) mol/l and 1 x 10(-7) mol/l) reduced the affinity of the fraction for Na+ and K+ to zero, while at 1 x 10(-6) mol/l, the affinity for the cations was almost as high a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969636
更新日期:1989-02-01 00:00:00
abstract::The influence of theophylline (2.5--100 mg/kg p.o.) on cyclic 3',5'-adenosine monophosphate (cAMP) and cyclic 3',5'-guanosine monophosphate (cGMP) in brain of Sprague-Dawley rats (0.5--3.0 hr after administration of theophylline) was investigated. It was found that theophylline increases cAMP and cGMP levels when admi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964436
更新日期:1979-10-01 00:00:00
abstract::Because it appears that oxidative stress and inflammation are implicated with disease pathogenesis in the diabetic brain, many researchers have used streptozotocin (STZ)-induced diabetic animals to study superoxide production and the effects of superoxide scavengers like Cu,Zn-superoxide dismutase (SOD1). However, man...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0280-6
更新日期:2011-01-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966203
更新日期:1990-05-01 00:00:00
abstract::Isozyme patterns of cholinesterase (ChE) from heart, tongue, and skeletal muscle of normal and dystrophic hamsters are presented. Two principal bands, bands 1 and 2, were evaluated. Band 1 migrates faster towards the anode than does band 2. While bands 1 and 2 stain for AChE and were found in control muscles, only ban...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964958
更新日期:1980-12-01 00:00:00
abstract::The stability of somatostatin (SS) in brain tissue was studied in human material obtained post-mortem and in the rat. In both human and rat brain, loss of SS was found to occur in tissue frozen to -70 degrees C. In the rat, this loss varied from 26 to 70 percent depending on the type of tissue processing used. These d...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973289
更新日期:1988-06-01 00:00:00
abstract::A set of synaptic membrane antigens has been investigated in a number of tissues by indirect immunofluorescence histochemistry, using an antiserum (SPM-I) raised against a purified synaptosomal plasma membrane fraction prepared from day-old chick forebrain. The antigens were found to be present in both the central and...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966022
更新日期:1977-02-01 00:00:00
abstract::The role of polyamines (PA) synthesis in NMDA receptor-mediated 45Ca2+ fluxes and norepinephrine release was studied in rat hippocampal synaptosomes. NMDA (50 microM) caused a sharp (> 2-fold) transient increase in PA synthesis regulating enzyme, ornithine decarboxylase (ODC) activity with concomitant elevation in PA ...
journal_title:Neurochemical research
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doi:10.1007/BF00972471
更新日期:1994-11-01 00:00:00
abstract::An analytical method for the determination of saturated very long chain (VLC) fatty acids in the serum has been devised. Free fatty acids obtained after hydrolysis of total lipid extracts were converted into p-bromophenacyl esters. The derivatives were purified in two sequential steps by clean-up on C18 reversed-phase...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968082
更新日期:1984-12-01 00:00:00
abstract::The study examined (a) whether there is sex difference in spinal cord and plasma oxidative stress profiles in Dark Agouti rats immunised for experimental autoimmune encephalomyelitis (EAE), the principal experimental model of multiple sclerosis, and (b) whether there is correlation between the oxidative stress in spin...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2094-7
更新日期:2017-02-01 00:00:00
abstract::The most important therapeutic effect of cholinesterase inhibitors (ChEI) on approximately 50% of Alzheimer's disease (AD) patients is to stabilize cognitive function at a steady level during a 1-year period of treatment as compared to placebo. Recent studies show that in a certain percentage (approximately 20%) of pa...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1022869222652
更新日期:2003-04-01 00:00:00
abstract::Previous studies suggest that alterations of brain glutamate synthesis and release occur in experimental thiamine deficiency. In order to assess the integrity of post-synaptic glutamatergic receptors in thiamine deficiency, binding sites for [3H]glutamate (displaced by NMDA), [3H]-kainate, and [3H]quisqualate (AMPA si...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00995378
更新日期:1995-10-01 00:00:00
abstract::Previous studies have shown that early life stress induced by maternal separation or non-handling can lead to behavioural deficits in rats and that these deficits can be alleviated by providing palatable cafeteria high-fat diet (HFD). In these studies we investigated the effects of maternal separation or non-handling ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-0985-4
更新日期:2013-04-01 00:00:00
abstract::Caspase activation and apoptotic events may take place in terminal regions far removed from the cell body and contribute to synapse loss in neurodegenerative diseases. For examination of events in terminals, we have developed a cell-free assay using quantitative flow cytometric analysis (fluorescence-activated cell so...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1019840417796
更新日期:2002-06-01 00:00:00
abstract::The present study has attempted to elucidate the alteration of serotonin turnover after 24 h REM sleep deprivation in different regions in brain of young rat. Sleep deprivation was induced by the inverted flower pot technique. Results of this study show increased serotonin turnover after 24 h REM sleep deprivation in ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-9139-7
更新日期:2006-01-01 00:00:00
abstract::Brain regional 5-HT (5-hydroxytryptamine) and 5-HIAA (5-hydroxyindole-3-acetic acid) concentrations in male LE rats at three times were measured by a fluorescence method, to evaluate effects of intracranial surgery and administration of melatonin on the changes in these compounds during the first part of the dark phas...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965929
更新日期:1989-10-01 00:00:00
abstract::Discrete populations of anti-S49 antibodies were found in the antisera of Lewis rats recovered from S49-induced experimental allergic encephalomyelitis (EAE). A potent inducer of EAE in Lewis rats, S49 is a synthetic peptide representing residues 69-84 of bovine myelin basic protein but with deletions at Gly-77 and Hi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00988601
更新日期:1985-12-01 00:00:00
abstract::Bovine brain synaptic membranes which were frozen and then extensively washed showed low affinity [3H]muscimol binding. These membranes contained GABA and calmodulin, apparently tightly bound within the membrane fraction. Membranes which were additionally treated with the detergent Triton X-100 showed high affinity [3...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970756
更新日期:1988-09-01 00:00:00