Abstract：
:Cathepsin B (EC 3.4.22.1) was purified 746-fold with a 21% recovery from bovine brain by autolysis, fractional precipitation with acetone, carboxy-methyl-Sephadex chromatography, affinity chromatography on a cystamine containing column and gel filtration chromatography. The purified cathepsin B eluted on gel filtration with an apparent molecular weight of 27,000 but was resolved into three bands of 30,000, 25,000 and 5,000 molecular weight by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). Antibodies to cathepsin B, raised against the 30,000 dalton band, were shown by immunoblots to react with both the 30,000 and 25,000 dalton proteins with results suggesting that the former predominated as the immunoreactive form in bovine brain homogenates. Isoelectric focusing demonstrated multiple bands, ranging from pH 4.75-5.2 with the major band at pH 5.1-5.2, all of which were capable of degrading N alpha-carbobenzoxy-L-arginyl-L-arginine 4-methoxy-beta-naphthylamide. The cathepsin B activity against N alpha-benzoyl-DL-arginine beta-naphthylamide (BANA) and bovine myelin basic protein (MBP) had a pH optimum of pH 6.0. The Km for the degradation of BANA was 1.0 mM and 5.1 mM when assayed in the presence of 1% and 2.5% dimethylsulfoxide, respectively. Cathepsin B from bovine brain has many properties similar to cathepsin B isolated from other organs. The degradative effect of cathepsin B on MBP suggests a role for this proteinase in inflammatory demyelination.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Bradley JD,Whitaker JNdoi
10.1007/BF00965209subject
Has Abstractpub_date
1986-06-01 00:00:00pages
851-67issue
6eissn
0364-3190issn
1573-6903journal_volume
11pub_type
杂志文章abstract：:The SAM strains, a group of related inbred strains consisting of senescence-prone inbred strains (SAMP) and senescence-resistant inbred strains (SAMR), have been successfully developed by selective inbreeding of the AKR/J strain of mice donated by the Jackson laboratory in 1968. The characteristic feature of aging com...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-009-9922-y
更新日期：2009-04-01 00:00:00
abstract：:There is evidence that many of the GABA(A) receptor subunits contain consensus sequence for tyrosine kinase, and phosphorylation may play a key role in ethanol's regulation of GABA(A) receptors. Recently, we investigated the effect of chronic exposure of ethanol (CE) on tyrosine kinase phosphorylation and reported tha...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9124-9
更新日期：2006-09-01 00:00:00
abstract：:Development of the central somatosensory system is profoundly modulated by the sensory periphery. Cauterization of facial whiskers alters the segregation pattern of barrels in rodents only during a few days just after birth (critical period). Although a molecular basis of the segregation of barrel neurons and the crit...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022075508176
更新日期：1997-07-01 00:00:00
abstract：:The lysosomal degradation of several sphingolipids requires the presence of four small glycoproteins called saposins, generated by proteolytic processing of a common precursor, prosaposin. Saposins share several structural properties, including six similarly located cysteines forming three disulfide bridges with the s...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022530508763
更新日期：1999-02-01 00:00:00
abstract：:Neuronal perikarya were isolated, using bulk preparative procedures, from bovine brains. Synaptosomes, neuronal perikarya, and brain homogenates had similar ganglioside patterns, with the synaptosomes containing at least four times more total ganglioside per mg protein than the neuronal perikarya and twice that of the...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966108
更新日期：1976-04-01 00:00:00
abstract：:Neuronal MHC class I proteins have been previously reported to regulate synaptic plasticity. Several reports indicate MHC class I proteins are expressed early during development of the nervous system, suggesting they may also play a role in neuronal development. Using cultured cortical neurons, we show MHC class I pro...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2676-7
更新日期：2019-02-01 00:00:00
abstract：:Blue light impinging on the many mitochondria associated with retinal ganglion cells (RGCs) in situ has the potential of eliciting necroptosis through an action on RIP1/RIP3 to stimulate RGC death in diseases like glaucoma and diabetic retinopathy. Cells in culture die when exposed to blue light. The death process is ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1946-5
更新日期：2016-09-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1970-5
更新日期：2016-10-01 00:00:00
abstract：:Deprenyl has been discovered by Knoll and co-workers. The R-enantiomer of deprenyl (selegiline) is a selective and irreversible inhibitor of the B-isoform of monoamine oxidase (MAO-B) enzyme. Due to its dopamine potentiating and possible neuroprotective properties it has an established role in the treatment of parkins...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0238-8
更新日期：2010-12-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964144
更新日期：1979-04-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022451224748
更新日期：1998-05-01 00:00:00
abstract：:The mechanism of focal demyelination in multiple sclerosis has been a long-standing enigma of this disorder. Cytokines, a diverse family of signalling molecules, are viewed as potential mediators of the process based on clinical observations and studies with animal models and tissue/cell culture systems. Myelin and ol...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022493013904
更新日期：1998-03-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964040
更新日期：1981-03-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1185-y
更新日期：2013-12-01 00:00:00
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journal_title：Neurochemical research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1023/b:nere.0000026402.09466.54
更新日期：2004-07-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964851
更新日期：1989-05-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0059-9
更新日期：2010-02-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966692
更新日期：1993-10-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966679
更新日期：1981-11-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-9969-9
更新日期：2009-10-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1783-y
更新日期：2016-05-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-0985-4
更新日期：2013-04-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0621-0
更新日期：2012-02-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965332
更新日期：1986-05-01 00:00:00
abstract：:Fecal incontinence and constipation still remain the major complications after procedures for anorectal malformations (ARMs). Previous studies have demonstrated a decrease of neural cell in lumbosacral spinal cord of ARMs patients and rat models. However, the underlying mechanism remains elusive. In this study, the ne...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2354-1
更新日期：2017-11-01 00:00:00
abstract：:A concise discussion of the role of different geometrical conformational states in the process of self-assembling of gangliosides is given. The report focuses on the effects of the geometrical variations occurring in the head group region of gangliosides as reflected on the geometrical properties of the whole assembly...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1020255529125
更新日期：2002-08-01 00:00:00
abstract：:α-Synuclein (α-Syn) plays a crucial role in the pathophysiology of Parkinson's disease (PD), the degeneration of dopaminergic neurons. Previous studies have shown that α-Syn regulates dopamine synthesis by binding to and inhibiting tyrosine hydroxylase (TH). In neurons, protein phosphatases (PPs) play a prominent role...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1477-x
更新日期：2015-03-01 00:00:00
abstract：:The pathogenesis of sepsis associated encephalopathy (SAE) is not yet clear: the blood-brain barrier (BBB) disruption has been indicated among the possible causative mechanisms. S100B, a calcium binding protein, originates in the central nervous system but it can be also produced by extra-cerebral sources; it is passi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9907-2
更新日期：2009-07-01 00:00:00
abstract：:Thiamine deficiency (TD) produces severe neurodegenerative lesions. Studies have suggested that primary neurodegenerative events are associated with both oxidative stress and inflammation. Very little is known about the downstream effects on intracellular signaling pathways involved in neuronal death. The primary aim ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-02975-7
更新日期：2020-04-01 00:00:00
abstract：:The effects of pre-treatment with ENA-713, an acetylcholinesterase (AChE) inhibitor, on changes in pre- and postsynaptic cholinergic indices in gerbil brain following transient ischemia were studied at 4 and 14 days after recirculation. In the ischemic group, hippocampal acetylcholine (ACh) level was significantly red...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966804
更新日期：1994-02-01 00:00:00
