Abstract：
:The vesicular hypothesis has stimulated fruitful investigations on many secreting systems. In the case of rapid synaptic transmission, however, the hypothesis has been found difficult to reconcile with a number of well established observations. Brief impulses of transmitter molecules (quanta) are emitted from nerve terminals at the arrival of an action potential by a mechanism which is under the control of multiple regulations. It is therefore not surprising that quantal release could be disrupted by experimental manipulation of a variety of cellular processes, such as a) transmitter uptake, synthesis, or transport, b) energy supply, c) calcium entry, sequestration and extrusion, d) exo- or endocytosis, e) expression of vesicular and plasmalemmal proteins, f) modulatory systems and second messengers, g) cytoskeleton integrity, etc. Hence, the approaches by "ablation strategy" do not provide unequivocal information on the final step of the release process since there are so many ways to stop the release. We propose an alternate approach: the "reconstitution strategy". To this end, we developed several preparations for determining the minimal system supporting Ca2+-dependent transmitter release. Release was reconstituted in proteoliposomes, Xenopus oocytes and transfected cell lines. Using these systems, it appears that a presynaptic plasmalemmal proteolipid, that we called mediatophore should be considered as a key molecule for the generation of transmitter quanta in natural synapses.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Dunant Y,Israël Mdoi
10.1023/a:1022451224748subject
Has Abstractpub_date
1998-05-01 00:00:00pages
709-18issue
5eissn
0364-3190issn
1573-6903journal_volume
23pub_type
杂志文章,评审abstract：:Donepezil is a selective inhibitor of acetylcholinesterase (AChE) clinically used for treating Alzheimer's disease. Cholinergic effects after short-term exposure of donepezil (up to 12 h) have been extensively studied in rats, but few have addressed the potential long-term effects. After 14 days administration (1x3 mg...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-8828-6
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
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doi：10.1007/s11064-014-1239-9
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0233-0
更新日期：2010-11-01 00:00:00
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journal_title：Neurochemical research
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更新日期：2021-01-13 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964696
更新日期：1983-05-01 00:00:00
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journal_title：Neurochemical research
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1206-x
更新日期：2014-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2007-04-01 00:00:00
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更新日期：2011-10-01 00:00:00
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更新日期：2015-10-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02532346
更新日期：1996-08-01 00:00:00
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更新日期：2003-05-01 00:00:00
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更新日期：1997-02-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2015-02-01 00:00:00
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journal_title：Neurochemical research
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更新日期：2011-07-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：2000-06-01 00:00:00
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更新日期：1978-02-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：1992-12-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968559
更新日期：1990-08-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
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更新日期：2020-01-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：2011-08-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966679
更新日期：1981-11-01 00:00:00
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更新日期：2017-05-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：2002-05-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：2015-08-01 00:00:00
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更新日期：2008-01-01 00:00:00
