Abstract:
:Prion diseases are conformational diseases, many factors are involved in altering the conformation of prion, such as RNA, DNA, pH, and copper etc. However the neurotoxic mechanism of prion diseases is not clear yet. The aim of this study is to investigate the effect of the nucleoprotein complex of RNA and recombinant ovine prion protein (OvPrP(C)) on the cultured rat cortical neurons in vitro. Our previous study revealed that the nucleoprotein complex (OvPrP(C)-RNA) is characterized with high β sheet conformation and proteinase K resistance. Here we found that the OvPrP(C)-RNA induced marked neuronal cell death by the MTT (3-(4,5-dimethyl-thiazole -2-yl)-2,5-diphenyl -tetrazolium bromide) and TUNEL (TdT mediated biotin-dUTP nicked-end labeling) assay, and the neurotoxic effects were confirmed by testing the content of Bcl-2 Associated X protein (Bax) in the immunoprecipitation assay and Western blot assay. Compared to the control group, there is no significant difference of active Bax or total Bax after RNA alone treatment or OvPrP(C) alone treatment, but the OvPrP(C)-RNA induced significant increases of active Bax level, while the contents of total Bax had no obvious changes after OvPrP(C)-RNA treatment. The results suggested that OvPrP(C)-RNA is neurotoxic in vitro, which added further evidence to the current understanding of mechanism of cellular injury by RNA molecules for transformation of the PrP(C) to PrP(Sc).
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Liu ML,Wen JJ,Xu XF,Zhao DMdoi
10.1007/s11064-011-0506-2subject
Has Abstractpub_date
2011-10-01 00:00:00pages
1863-9issue
10eissn
0364-3190issn
1573-6903journal_volume
36pub_type
杂志文章abstract::Turnover rate of individual molecular species of sphingomyelin of adult rat brain myelin and microsomal membranes was determined after an intracerebral injection of 100 microCi of [C3H3]choline. Myelin and microsomal membrane sphingomyelins were isolated from the rest of the lipids. The individual molecular species of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964414
更新日期:1981-10-01 00:00:00
abstract::The anterograde axonal transport of choline-phosphoglycerides was studied in sciatic nerve motoneurons of adult (3-month-old) and aged (24-month-old) rats. After the spinal cord injection of [2-3H]glycerol, choline-phosphoglycerides; the major phospholipid class was transported along the nerve. The axonal transport ra...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971365
更新日期:1987-01-01 00:00:00
abstract::Gangliosides, the acidic glycosphingolipids (GSLs) containing N-acetylgalactosamine and sialic acid are ubiquitous in the central nervous system. At least six DSL-glycosyltransferase activities (GLTs Gangliosides, the acidic glycosphingolipids (GSLs) containing N-acetylgalactosamine and sialic acid (or NAc-Neuraminic ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0762-9
更新日期:2012-06-01 00:00:00
abstract::It is thought that changes in gene expression in the brain mediate chronic ethanol-induced complex behaviors such as tolerance, dependence, and sensitization, and also relate to ethanol-induced brain toxicity. Using high-density filter-based cDNA microarrays (GeneFilters), we analyzed the expression of over 5000 genes...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020937728506
更新日期:2002-10-01 00:00:00
abstract::The standard ELISA technique was improved for the detection of antigalactocerebroside antibody in biological fluid. Mouse monoclonal antigalactocerebroside antibody was used to demonstrate specificity and sensitivity of the technique. After optimization of the assay, the usefulness of this measurement for the evaluati...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971533
更新日期:1988-03-01 00:00:00
abstract::Activation of cholinergic muscarinic receptors results in an increased turnover of membrane inositol phospholipids. In rat cerebral cortex slices, carbachol- and acetylcholine-induced inositol phosphates ([3H]InsPs) accumulation is maximal in 7 day-old rats and lowest in adults, while the density of muscarinic binding...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969181
更新日期:1990-01-01 00:00:00
abstract::Several studies showed that the up-regulation of glial glutamate transporter-1 (GLT-1) participates in the acquisition of brain ischemic tolerance induced by cerebral ischemic preconditioning or ceftriaxone pretreatment in rats. To explore whether GLT-1 plays a role in the acquisition of brain ischemic tolerance induc...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0639-3
更新日期:2012-03-01 00:00:00
abstract::The importance of astrocytic K(+) uptake for extracellular K(+) ([K(+)](e)) clearance during neuronal stimulation or pathophysiological conditions is increasingly acknowledged. It occurs by preferential stimulation of the astrocytic Na(+),K(+)-ATPase, which has higher K(m) and V(max) values than its neuronal counterpa...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0938-3
更新日期:2013-03-01 00:00:00
abstract::The consumption of flavonoid-rich foods, in particular fruits and vegetables, has been epidemiologically associated with a reduced risk of heart disease, neurodegenerative disease, cancer and other chronic diseases. Flavonoid glycosides, the main class of flavonoids, have been shown to exert CNS-mediated activities, p...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-9969-9
更新日期:2009-10-01 00:00:00
abstract::The conversion of phosphoethanolamine to phosphocholine requires 3 separate N-methyltransferases. We had previously purified the enzyme catalyzing the last methylation, phosphodimethylethanolamine N-methyltransferase. We have successfully purified the enzyme catalyzing the initial methylation of phosphoethanolamine. A...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00995388
更新日期:1995-10-01 00:00:00
abstract::Planarians are rapidly developing into very useful research subjects in pharmacology and neuroscience research. Here we report that curare, a cholinergic nicotinic receptor antagonist, alleviates the nicotine-induced planarian seizure-like movements (pSLM) by up to 50 % at equimolar concentrations of nicotine and cura...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1512-6
更新日期:2015-10-01 00:00:00
abstract::We previously reported that vanilloid receptor type 1 (VR1, or TRPV1) was up-regulated in dorsal root ganglion (DRG) and the spinal dorsal horn after chronic inflammatory pain produced by complete Freund's adjuvant (CFA) injection into the plantar of rat hind paw. In the present study, we found that subcutaneous or in...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9751-4
更新日期:2008-10-01 00:00:00
abstract::Tumor Necrosis Factor Receptor Super Family 6 gene (TNFRSF6), also known as FAS, encodes the Fas antigen, a cell surface receptor mediating cell apoptosis, situated on chromosome 10q located near the region of linkage to sporadic Alzheimer's disease (sAD). FAS levels have been reported elevated in the brain of AD pati...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9329-6
更新日期:2007-09-01 00:00:00
abstract::Endogenous phosphorylation of proteins from rat brain synaptosomal plasma membranes was studied in vitro. Cyclic AMP (cAMP) markedly stimulated(32)P incorporation in three protein bands with molecular weights of 75,000, 57,000, and 54,000, respectively. The effect of the behaviorally active peptide ACTH1-24 on this en...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965607
更新日期:1976-12-01 00:00:00
abstract::Propofol and ketamine may provide certain degree of neuroprotection, but the underlying mechanism remains unclear to date. The cAMP response element-binding protein (CREB) was proposed that its phosphorylation at Ser133 (P-CREB) constituted a convergence point involved in neuroprotection. The purpose of this study was...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0582-3
更新日期:2012-01-01 00:00:00
abstract::Several lines of evidence indicate that ATP may play an important role in Long-Term Potentiation. In this investigation we evaluated the effect of a memory task (step-down inhibitory avoidance) on the synaptosomal ecto-enzymes (ATP diphosphohydrolase and 5'-nucleotidase) involved in the degradation of ATP to adenosine...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1021084422228
更新日期:1998-07-01 00:00:00
abstract::Isozyme patterns of cholinesterase (ChE) from heart, tongue, and skeletal muscle of normal and dystrophic hamsters are presented. Two principal bands, bands 1 and 2, were evaluated. Band 1 migrates faster towards the anode than does band 2. While bands 1 and 2 stain for AChE and were found in control muscles, only ban...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964958
更新日期:1980-12-01 00:00:00
abstract::Natural products including those derived from plants, have over the years greatly contributed to the development of therapeutic drugs. Polygodial and drimanial are sesquiterpenes isolated from the bark of the plant Drymis Winteri (Winteraceae) that exhibit antinociceptive properties. Since peripheral glutamate present...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-9033-3
更新日期:2006-03-01 00:00:00
abstract::Incomplete ischemia of the spinal cord was produced in dogs by 40 min occlusion of the abdominal aorta that was followed by 5-40 min of recirculation. Amino acid incorporation into ribosomes in vitro in the presence of venous blood sera was estimated. The most significant reduction in incorporation was produced by ser...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965210
更新日期:1986-06-01 00:00:00
abstract::"Kindling" is a phenomenon of epileptogenesis, which has been widely used as an experimental model of temporal lobe epilepsy. At the present work we investigated the contribution of NMDA receptors in the Pentylenetetrazol-induced "kindling" model in the mouse brain, by using quantitative autoradiography and the radioa...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1021143813935
更新日期:1999-12-01 00:00:00
abstract::In our previous study vesamicol, an inhibitor of the acetylcholine transporter of the cholinergic vesicles, inhibited veratridine-evoked external Ca(2+)-dependent acetylcholine release from striatal slices but did not influence acetylcholine release observed in Ca(2+)-free medium (4). Here we examined if the effect of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968780
更新日期:1992-06-01 00:00:00
abstract::Silibinin has been widely used to treat liver diseases due to its antioxidant activity. However, the effects of silibinin on the central nervous system have not been thoroughly investigated. The pathological hallmarks of Alzheimer's disease are the accumulation of amyloid β protein, development of neurofibrillary tang...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2389-3
更新日期:2017-12-01 00:00:00
abstract::Cholinesterase activities in rat forebrain, erythrocytes, and plasma were assessed after a single oral administration of metrifonate or dichlorvos. In 3-month-old rats, the dichlorvos (10 mg/kg p.o.)induced inhibition of cholinesterase reached its peak in brain after l5-45 min and after 10-30 min in erythrocytes and p...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02531650
更新日期:1996-03-01 00:00:00
abstract::It has been established that oligodendrocytes, the myelin forming cells, participate in iron homeostasis through the synthesis and secretion of transferrin. Here we investigated whether a correlation exists between myelination, the commonly studied function of oligodendrocytes, and that of transferrin synthesis and se...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-1826-2
更新日期:1999-02-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a degenerative disorder caused by motor neuron loss. T-cell intracellular antigen-1 (TIA-1), a cytotoxic T lymphocyte granule-associated RNA binding protein, is a key component of stress granules. However, it remains uncertain whether ALS-causing superoxide dismutase-1 (SOD1) tox...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03137-5
更新日期:2020-12-01 00:00:00
abstract::Hypoxia-induced oxidative stress is one of the major hallmark reasons underlying brain dysfunction. In the present manuscript, we have used CoCl2-induced hypoxic mice to investigate alterations in the activities of chief antioxidative stress enzymes- superoxide dismutase (SOD) and catalase (CAT) and expression of thei...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1388-x
更新日期:2014-09-01 00:00:00
abstract::There is growing evidence that ketone bodies (KB)-derived from fatty acid oxidation and produced during fasting or consumption of high-fat diets-can exert broad neuroprotective effects. With respect to epilepsy, KB (such as β-hydroxybutyrate or BHB, acetoacetate and acetone) have been shown to block acutely induced an...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-017-2253-5
更新日期:2017-07-01 00:00:00
abstract::Autoantibodies to three eukaryotic 60S ribosomal phosphoproteins P0, P1 and P2 have been found in the sera of 10-20% of patients with systemic lupus erythematosus (SLE). These antibodies inhibit protein synthesis in vitro and when microinjected into cultured human fibroblasts. The three proteins share a common epitope...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969925
更新日期:1990-04-01 00:00:00
abstract::Turmeric has been in use since ancient times as a condiment and due to its medicinal properties. Curcumin, the yellow colouring principle in turmeric, is polyphenolic and major active constituent. Besides anti-inflammatory, thrombolytic and anticarcinogenic activities, curcumin also possesses strong antioxidant proper...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9547-y
更新日期:2008-06-01 00:00:00
abstract::The number of c-Fos protein-like immunoreactive (c-Fos-IR) neurons in the spinal dorsal horn evoked by noxious stimulation was previously shown to be increased following peripheral nerve injury, and this increase was proposed to reflect the neuropathic pain state. The aim of this study was to investigate whether anoma...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1484-y
更新日期:2015-03-01 00:00:00