Abstract：
:Brains of Alzheimer disease patients in early stages of dementia contain an increased 24(S)-hydroxycholesterol (cerebrosterol)/cholesterol ratio when compared to controls. In this study, effects of amyloid beta peptides and of racemic 24-hydroxycholesterol were evaluated in vitro on undepleted or cholesterol-depleted hippocampal synaptosomes of young and old rats via a high-affinity choline transport and membrane anisotropy measurements. Depletion of membrane cholesterol decreased the transport of [3H]choline, increased the specific binding of [3H]hemicholinium-3 and decreased membrane anisotropy. However, less alterations were found in old when compared to young brains. 500 nM nonaggregated peptides were ineffective but aggregated fragment 1-42 evoked marked drops in the transport and anisotropy values on depleted synaptosomes. 50 microM 24-hydroxycholesterol inhibited choline transport on depleted synaptosomes but it did not influence membrane anisotropy. Peptides eliminated the actions of oxysterol on choline carriers in young but not in old rats. On the other hand, oxysterol eliminated the effects of peptides on membrane anisotropy. Our study suggests a possible role of membrane cholesterol in the regulation of choline carriers and supports data reporting a protective role of membrane cholesterol against toxic effects of amyloid beta peptides. Moreover, via Raman spectroscopy we demonstrate for the first time that peptides form a complex with 24-hydroxycholesterol.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Kristofiková Z,Kopecký V Jr,Hofbauerová K,Hovorková P,Rípová Ddoi
10.1007/s11064-007-9443-5subject
Has Abstractpub_date
2008-03-01 00:00:00pages
412-21issue
3eissn
0364-3190issn
1573-6903journal_volume
33pub_type
杂志文章abstract：:Oxidative stress-induced neuronal cell death requires opening of the mitochondrial permeability transition pore. P53 mitochondrial translocation and association with Cyclophilin D (Cyp-D) is required for the pore opening. Here we tested this signaling axis in oxygen glucose deprivation (OGD)/re-oxygenation-induced in ...
journal_title：Neurochemical research
pub_type： 杂志文章
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abstract：:Postmortem changes in the activity of GDH, GAD, GABA-T, and GS in the adult forebrain and cerebellum and in the forebrain of newborns have been investigated. The activities of GDH, GAD, and GS were stable till 12 hr after death in both adult and young brain regions studied. The activity of GABA-T was stable till 30 mi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966226
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pub_type： 杂志文章
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journal_title：Neurochemical research
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更新日期：2012-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-08-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1990-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00965323
更新日期：1986-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1023/b:nere.0000018839.59457.5c
更新日期：2004-04-01 00:00:00
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