Abstract：
:The role of protein phosphorylation in catecholamine secretion from bovine adrenomedullary chromaffin cells was studied using different protein kinase inhibitors. Naphthalenesulfonamide derivatives as ML9 and ML7, more specific for the myosin light chain kinase, and the calmodulin antagonist W7 inhibited catecholamine secretion 20 and 40% respectively in digitonin-permeabilized chromaffin cells. ML9 also decreased calcium evoked protein phosphorylation of different proteins including tyrosine hydroxylase in permeabilized cells. These naphthalenesulfonamide derivatives showed also an effect in intact cells, ML9 and W7 produced 50% inhibition in catecholamine secretion and 45Ca2+ uptake, however H8 had no effect. The partial [3H]nitrendipine binding displacement of these drugs to adrenomedullary membranes suggests that these sulfonamide derivatives could interact directly with L-type calcium channels in intact cells. The results obtained in permeabilized cells suggest a possible role of protein phosphorylation in the regulation of catecholamine secretion in chromaffin cells.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Reig JA,Viniegra S,Ballesta JJ,Palmero M,Guitierrez LMdoi
10.1007/BF00969089subject
Has Abstractpub_date
1993-03-01 00:00:00pages
317-23issue
3eissn
0364-3190issn
1573-6903journal_volume
18pub_type
杂志文章abstract：:Antibodies to synthetic myelin basic protein peptide S82 (TTHYG-SLPQKAQGHRPQDEG) did not react with synthetic peptide S8 (GSLPQKAQGHRPQDENG) and only partially so with synthetic peptide S79 (AQGHRPQDEG); however, the antibodies did react to a considerable extent with an equimolar mixture of S8 and S79. Since the anti-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964671
更新日期：1984-10-01 00:00:00
abstract：:Astrocyte-neuron communication has recently been proposed as a potential mechanism participating to synaptic transmission. With the development of this concept and accumulating evidences in favor of a modulation of synaptic transmission by astrocytes, has emerged the term gliotransmission. It refers to the capacity of...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-012-0807-0
更新日期：2012-11-01 00:00:00
abstract：:The potassium-stimulated release of acetylcholine, norepinephrine, serotonin, glutamate, and 4-aminobutyrate from superfused rat cortical slices was studied during hypoxia. A reduction in oxygen tensions from 603 +/- 6 to 22 +/- 2 mm Hg selectively altered the calcium-dependent efflux of these neurotransmitters, but d...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964800
更新日期：1984-08-01 00:00:00
abstract：:Alzheimer's disease (AD) is a common neurodegenerative condition that affects the elderly population. Its primary symptom is memory loss. The memory dysfunction in AD has been associated with cortical cholinergic deficiency and loss of cholinergic neurons of the nucleus basalis of Meynert (NBM). Zizyphus jujube (ZJ) a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1232-8
更新日期：2014-02-01 00:00:00
abstract：:Alterations in lysosomal proteases have been implicated in many neurodegenerative diseases. The current study demonstrates a concentration-dependent decrease in PC12 cell viability and transient changes in cystatin C (CYSC), cathepsin B (CATB), cathepsin D (CATD) and caspase-3 following exposure to H2O2. Furthermore, ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9338-5
更新日期：2007-09-01 00:00:00
abstract：:Senescence of the central nervous system is characterized by a progressive loss of neurons that can result in physiological and behavioral impairments. Reduction in the levels of central neurotrophic factors or of neurotrophin receptors may be one of the causes of the onset of these degenerative events. Thus, a proper...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00995145
更新日期：1995-01-01 00:00:00
abstract：:Proteolipid protein (PLP) is the major intrinsic membrane protein of CNS myelin and is expressed in oligodendrocytes as part of a coordinate program of myelin-specific gene activation. In order to identify the DNA sequences and proteins involved in the regulation of PLP transcription, we have analyzed the 5' flanking ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968716
更新日期：1994-08-01 00:00:00
abstract：:The uptake of radioactive ethanolamine has been studied in exclusively neuronal and glial cell cultures from dissociated cerebral hemispheres of chick embryos. Both cell types show saturable kinetics; neurons have an apparent Km of 6.7 microM, Vmax 41.4 pmol mg prot.-1 min-1 and glial cells a Km of 119.6 microM, Vmax ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965162
更新日期：1986-01-01 00:00:00
abstract：:Apoptosis is a highly complex form of cell death that can be triggered by alterations in Ca(2+) homeostasis. Members of the Bcl-2 family may regulate apoptosis and modulate Ca(2+) distribution within intracellular compartments. Bax, a proapoptotic member of the family, is constitutively expressed and soluble in the cy...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0411-8
更新日期：2011-05-01 00:00:00
abstract：:The purpose of this study was to investigate whether the kappa-opioid receptor (KOR) agonist, BRL52537, has a neuroprotective effect against cerebral ischemia/reperfusion (I/R) injury in rats and further explore the underlying mechanisms. Adult male Sprague-Dawley rats were randomly assigned into sham (group A), I/R (...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1139-4
更新日期：2013-11-01 00:00:00
abstract：:Myelinated axons were isolated by flotation from bovine pons, middle cerebellar peduncle, cervical spinal cord and three regions of the subcortical white matter. The myelinated axons were osmotically and mechanically shocked, followed by fractionation on a linear 15% sucrose to 45% sucrose density gradient. Axolemma-e...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01268880
更新日期：1988-05-01 00:00:00
abstract：:We propose the following scheme for cerebral uptake and overall metabolism of glucose in vivo: that brain selects from two pools of glucose anomers in arterial blood, that it takes up excess glucose, that glucose enters the brain tissue as glucose-6-phosphate through the actions of mutarotase and hexokinase, that some...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964568
更新日期：1985-02-01 00:00:00
abstract：:There is increasing evidence to suggest that hepatic encephalopathy in acute liver failure is the result of altered glutamatergic function. In particular, the high affinity uptake of glutamate is decreased in brain slices and synaptosomes from rats with acute liver failure as well as by exposure of cultured astrocytes...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022532623281
更新日期：1999-11-01 00:00:00
abstract：:Several epidemiological studies have shown that consumption of large quantities of vegetables especially cruciferous vegetables (Broccoli and Brussels sprouts) can protect against chronic diseases. Sulforaphane, an isothiocynate found in cruciferous vegetables has been demonstrated to have neuroprotective effects in s...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1965-2
更新日期：2016-10-01 00:00:00
abstract：:Nerve growth factor (NGF) has been isolated from human placental tissue. Using the chicken embryo dorsal root ganglia assay, we determined levels of NGF activity for the amnion, placental cotyledons, cord serum, fetal serum, and maternal serum. The highest levels of NGF activity were measured in placental cotyledons. ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964058
更新日期：1978-04-01 00:00:00
abstract：:Lewy bodies and Lewy neurites in the brain constitute the main histopathological features of Parkinson's disease (PD) and dementia with Lewy bodies. They comprise amyloid-like fibrils composed of α-synuclein (αS), a small protein (~14 kDa). Because the aggregation of αS in the brain has been implicated as a critical s...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2382-x
更新日期：2017-12-01 00:00:00
abstract：:Due to the biological importance of sodium and its relative scarcity within many natural environments, 'salt appetite' has evolved whereby dietary salt is highly sought after and palatable when tasted. In addition to peripheral responses, salt depletion is detected within the brain via circumventricular organs and 11β...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2336-3
更新日期：2018-01-01 00:00:00
abstract：:Antipsychotics represent high affinity for sigma receptors and sigma-like drugs often have the psychotomimetic properties. Besides, the receptors are unevenly distributed in human brain. These findings suggest that sigma receptors might be involved in the pathophysiology of schizophrenia. Sigma receptors in rat and hu...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966825
更新日期：1992-10-01 00:00:00
abstract：:The aggravated risk on intracerebral hemorrhage (ICH) with drugs used for stroke patients should be estimated carefully. We therefore established sensitive quantification methods and provided a rat ICH model for detection of ICH deterioration. In ICH intrastriatally induced by 0.014-unit, 0.070-unit, and 0.350-unit co...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022826220469
更新日期：2003-05-01 00:00:00
abstract：:The specific molecular events that underlie the age-related loss of cognitive function are poorly understood. Although not experimentally substantiated, age-dependent neuronal loss has long been considered central to age-related cognitive decline. More recently, age-related changes in brain white matter have taken pre...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-007-9341-x
更新日期：2007-12-01 00:00:00
abstract：:Donepezil is a selective inhibitor of acetylcholinesterase (AChE) clinically used for treating Alzheimer's disease. Cholinergic effects after short-term exposure of donepezil (up to 12 h) have been extensively studied in rats, but few have addressed the potential long-term effects. After 14 days administration (1x3 mg...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-8828-6
更新日期：2005-12-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common form of dementia in the aged population. Early-onset familial AD (FAD) involves mutations in a gene on chromosome 21 encoding the amyloid protein precursor or on chromosomes 14 or 1 encoding genes known as presenilins. All mutations examined have been found to increase the p...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022471729291
更新日期：1998-05-01 00:00:00
abstract：:We investigated the distribution of serotonin (5-HT) receptors of type 3 (5-HT3) in human brain areas, by means of the the specific binding of [3H]GR65630. The brains were obtained during autoptic sessions from 6 subjects. Human brain membranes and the binding of [3H]GR65630 were carried out according to standardized ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1010939530412
更新日期：2001-03-01 00:00:00
abstract：:Serotonin-synthesizing raphé/parapyramidal neurons (5-HT neurons) may function as sympathetic premotor neurons regulating sympathetic outflow to the cutaneous vascular bed. In the present study a genetically engineered pseudorabies virus (PRV) expressing green fluorescent protein (GFP) was injected into the rat tail. ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-9018-2
更新日期：2006-02-01 00:00:00
abstract：:Association of the candidate gene DLST with late-onset Alzheimer's disease (LOAD) risk has been suggested on the basis of case-control studies. This gene, located on chromosome 14q24.3, encodes a subunit of a mitochondrial component known to be defective in AD, the alpha-ketoglutarate dehydrogenase complex. Positive r...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000014833.54481.1d
更新日期：2004-03-01 00:00:00
abstract：:Previous studies have shown that chronic administration of SR-3 (a 1:4 mixture of alpha-linolenic and linoleic acid) affects spatial learning, thermoregulation, pain threshold and protection from seizures. The mode of action is unknown. One possible explanation is that the preparation induces changes in the fatty acid...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022430620205
更新日期：1998-05-01 00:00:00
abstract：:One hour incubation of rat cortical slices in a medium without oxygen and glucose (oxygen-glucose deprivation, OGD) increased S100B release to 6.53 ± 0.3 ng/ml/mg protein from its control value of 3.61 ± 0.2 ng/ml/mg protein. When these slices were then transferred to a medium containing oxygen and glucose (reoxygenat...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1301-7
更新日期：2014-07-01 00:00:00
abstract：:Cell surface β-Amyloid precursor protein (APP) is known to have a functional role in iron homeostasis through stabilising the iron export protein ferroportin (FPN). Mechanistic evidence of this role has previously only been provided through transcriptional or translational depletion of total APP levels. However, numer...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02747-y
更新日期：2019-06-01 00:00:00
abstract：:A synthetic peptide corresponding to the first 28 amino acids of the Alzheimer disease amyloid beta/A4 peptide (3.2 kDa) aggregated to a high molecular weight (15 kDa) on SDS/urea polyacrylamide gels. Proteinase K, V8 protease, trypsin, and endopeptidase Lys-C readily degraded the aggregate. By contrast, when digested...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00974579
更新日期：1992-04-01 00:00:00
abstract：:The overlapping distribution of opioid and cholecystokinin (CCK) peptides and their receptors (mu and delta opioid receptors; CCK-A and CCK-B receptors) in the central nervous system have led to a large number of studies aimed at clarifying the functional relationships between these two neuropeptides. Most of the phar...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF02532381
更新日期：1996-11-01 00:00:00
