Abstract:
:Cationic lipid formulations consisting of 3beta [N-(N', N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) and the helper lipid dioleoylphosphatidylethanolamine (DOPE) (1.5: 1 molar ratio) were prepared by solvent evaporation and sized by high pressure extrusion. Liposomes made of 1:1 molar ratio 1 ,2-dioleoyl-3-trimethyl-ammonium-propane (DOTAP)/DOPE were used as controls in the study. The two formulations were characterized and evaluated for their efficiency in transfecting SKnSH (neuroblastoma) and primary rat neuronal cell lines. DC-Chol/DOPE liposomes were more efficient at transfecting both the SKnSH and the primary rat neuronal cells and also less toxic compared to the DOTAP/DOPE liposomes. The cellular-associated signal of rhodamine-labeled DC-Chol/DOPE liposomes into SKnSH and primary rat neuronal cells was higher than the rhodamine-labeled DOTAP/DOPE liposomes. These results demonstrate that DC-Chol/DOPE cationic liposomes provide an efficient vehicle for the delivery of plasmids into SKnSH and primary neuronal cells compared to DOTAP/DOPE liposomes. DC-Chol/DOPE liposomes may provide a good non-viral candidate for transfecting primary rat neuronal cells.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Ajmani PS,Hughes JAdoi
10.1023/a:1021012727796keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
699-703issue
5eissn
0364-3190issn
1573-6903journal_volume
24pub_type
杂志文章abstract::Developing cellular models of sporadic Alzheimer's disease (sAD) is challenging due to the unknown initiator of disease onset and the slow disease progression that takes many years to develop in vivo. The use of human induced pluripotent stem cells (iPSCs) has revolutionised the opportunities to model AD pathology, in...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-018-2663-z
更新日期:2018-12-01 00:00:00
abstract::The primary objective of this investigation was to assess the neuroprotective efficacy of spice active principles namely Eugenol (Eug) and isoeugenol (IE) in an acrylamide (ACR) neuropathy model in rats. In the present study, ACR administration (50 mg/kg bw, i.p. 3 times/week) for 5 weeks to growing rats caused typica...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0924-9
更新日期:2013-02-01 00:00:00
abstract::N-terminal protein arginylation has been demonstrated in vitro and in situ and has been reported to increase following injury to sciatic nerves of rats. The present study attempts to demonstrate these reactions in vivo by applying [3H]Arg to the cut end of sciatic nerves in anesthetized rats and assaying for N-termina...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1021998227237
更新日期:1997-12-01 00:00:00
abstract::An analytical method for the determination of saturated very long chain (VLC) fatty acids in the serum has been devised. Free fatty acids obtained after hydrolysis of total lipid extracts were converted into p-bromophenacyl esters. The derivatives were purified in two sequential steps by clean-up on C18 reversed-phase...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968082
更新日期:1984-12-01 00:00:00
abstract::Several lines of evidence suggest that the mechanism underlying drug-induced neuronal apoptosis is initiated by the increased production of reactive oxygen species (ROS). 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), a neurotoxin, has been shown to initiate an apoptotic cascade by increasing ROS in the dopamine...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1208-8
更新日期:2014-02-01 00:00:00
abstract::Protein kinase C has recently attracted considerable attention because of its importance in the control of cell division, cell differentiation, and signal transduction across the cell membrane. The activity of this enzyme is altered by several lipids such as diacylglycerol, free fatty acids, lipoxins, gangliosides, an...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00973288
更新日期:1988-06-01 00:00:00
abstract::There is increasing evidence that proteins normally involved in the cell cycle play a role in the regulation of neuronal apoptotic death following various insults. However, it is not clear if the same mechanisms regulate cell death of oligodendrocytes as well. In this study, we investigated the mechanism of ceramide-i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000021236.32785.37
更新日期:2004-05-01 00:00:00
abstract::The importance of astrocytic K(+) uptake for extracellular K(+) ([K(+)](e)) clearance during neuronal stimulation or pathophysiological conditions is increasingly acknowledged. It occurs by preferential stimulation of the astrocytic Na(+),K(+)-ATPase, which has higher K(m) and V(max) values than its neuronal counterpa...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0938-3
更新日期:2013-03-01 00:00:00
abstract::Because it appears that oxidative stress and inflammation are implicated with disease pathogenesis in the diabetic brain, many researchers have used streptozotocin (STZ)-induced diabetic animals to study superoxide production and the effects of superoxide scavengers like Cu,Zn-superoxide dismutase (SOD1). However, man...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0280-6
更新日期:2011-01-01 00:00:00
abstract::To clarify the molecular basis of the cytoprotective properties of immunophilin ligands (IPLs), the anti-apoptotic effects of IPLs were determined in human glioma U251 cells. GPI1046 and V10367, non-immunosuppressive IPLs (NI-IPLs), as well as FK506, an immunosuppressive IPL (I-IPL), had cytoprotective effects against...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000029565.92587.25
更新日期:2004-08-01 00:00:00
abstract::We report further analysis of axonally transported proteoglycans in soluble and membranous subfractions of goldfish optic tectum. Distribution of transported 35SO4 radioactivity was 35.2% soluble, 63.4% Triton-NaCl extractable and 1.4% unextracted. Proteoglycans isolated on DEAE cellulose were treated with chondroitin...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970543
更新日期:1995-02-01 00:00:00
abstract::Trans-caryophyllene (TC), a component of essential oil found in many flowering plants, has shown its neuroprotective effects in various neurological disorders. However, the effects of TC on epilepsy haven't been reported before. In this study, we investigated the effect of TC on kainic acid-induced seizure activity ca...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1474-0
更新日期:2015-01-01 00:00:00
abstract::Ingestion of ammonium induces hyperammonemia which increases tubulin content in cerebrum but not in cerebellum. We have dissected 11 discrete areas of cerebrum and quantified the tubulin content in control and hyperammonemic rats. An heterogeneity in the induction of tubulin is shown. The areas more affected are ventr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965516
更新日期:1989-12-01 00:00:00
abstract::Members of the PDGF family have multiple roles during embryogenesis and in a variety of pathological situations in the adult. One of the major sites of PDGF-B expression in adult mammals are postmitotic CNS neurons. Combined with reported neurotrophic and neuroprotective effects of exogenously administered PDGFs, this...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022421001288
更新日期:2003-02-01 00:00:00
abstract::The labeling of RNA in young and adult rat brain has been studied by measuring in vitro (tissue slices incubation) the incorporation of labeled uridine into RNA of total tissue and of the various subcellular fractions purified from cerebral hemispheres of 1- and 10-month-old rats. Gel electrophoretic analysis of the n...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965100
更新日期:1983-04-01 00:00:00
abstract::We analyzed two disease model groups with rats infected by Japanese encephalitis virus (JEV), a 90-day group and a 180-day group after JEV infection. The time measured by the modified pole test showed that motor activities in these two groups were slower than those of age-matched control groups. Striatal dopamine (DA)...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9197-5
更新日期:2006-12-01 00:00:00
abstract::The microdialysis technique was utilized to study the local effects of D1 and D2 family type dopamine (DA) receptor (R) ligands on the in vivo release of endogenous glutamate (GLU) and aspartate (ASP) from rat substantia nigra (SN). Addition to the dialysis perfusion solution of either D1-R and D2-R agonists, such as ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970540
更新日期:1995-02-01 00:00:00
abstract::We have previously showed that peptide neurotensin inhibits neuronal Na(+), K(+)-ATPase activity, an effect which involves high affinity neurotensin receptor. Nitric oxide (NO) acts as a neurotransmitter or as a neuromodulator when it is synthesized by neuronal nitric oxide synthase. Neurotensin effect on Na(+), K(+)-...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0552-9
更新日期:2011-12-01 00:00:00
abstract::Cholinergic muscarinic receptors, when stimulated by arecoline, can activate cytosolic phospholipase A(2) (cPLA(2)) to release arachidonic acid (AA) from membrane phospholipid. This signal can be imaged in the brain in vivo using quantitative autoradiography following the intravenous injection of radiolabeled AA, as a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9372-3
更新日期:2007-11-01 00:00:00
abstract::Abnormal myo-[2-3H]inositol incorporation into phosphatidylinositol has been found in phentolamine-treated synaptosomes that were isolated from the cerebral hemispheres of galactose toxic rats and incubated with [33P]Pi and myo-[2-3H] inositol. In galactose toxic rats phentolamine-stimulated myo-[2-3H]inositol labelin...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965068
更新日期:1982-01-01 00:00:00
abstract::Proteasome inhibition has been observed in many neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Here, the effect of proteasome inhibition on the morphology of cultured rat cortical astrocytes was investigated. Increasing evidence suggests that the function of astrocytes is related close...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9830-6
更新日期:2009-05-01 00:00:00
abstract::Alzheimer's disease (AD) is the most common type of neurodegenerative dementia that affects the elderly population. Nerve growth factor (NGF) contributes to the survival, regeneration and death of neurons during aging and in neurodegenerative diseases. Recently, research has shown that NGF is related to the pathology,...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-016-1829-9
更新日期:2016-06-01 00:00:00
abstract::Our previous studies have shown that three sigma (sigma) receptor ligands, (+)-N-allylnormetazocine ((+)-SKF-10,047), (+/-)-pentazocine and 1,3-di(2-tolyl)guanidine (DTG) differently regulated the dopamine (DA) transmission in the rat brain. In the present study, we attempted to clarify the role of sigma 1 receptor su...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027361101419
更新日期:1997-09-01 00:00:00
abstract::Recent studies have demonstrated a scrutinized association of diabetes mellitus with depressive symptoms and major depression. Glycogen synthase kinase-3 (GSK-3) is a protein kinase enzyme constitutively active in non-stimulated cells and in multiple signalings. Independent lines of research provide a converging evide...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1670-6
更新日期:2015-09-01 00:00:00
abstract::Rett syndrome (RS) is an X-linked neurodevelopmental disorder mostly involving mutations in the gene for methyl-CpG-binding protein 2 (MECP2). Ganglioside abnormalities were previously found in cerebrum and cerebellum in RS patients. We evaluated total lipid distribution in cerebrum/brainstem, hippocampus, and cerebel...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9874-7
更新日期:2009-06-01 00:00:00
abstract::The p53 tumor suppressor gene is a sequence-specific transcription factor that activates the expression of genes engaged in promoting growth arrest or cell death in response to multiple forms of cellular stress. p53 expression is elevated in damaged neurons in acute models of injury such as ischemia and epilepsy and i...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1021687810103
更新日期:2003-01-01 00:00:00
abstract::Microglia, the resident immune cells of the CNS, are primary regulators of the neuroimmune response to injury. Type I interferons (IFNs), including the IFNαs and IFNβ, are key cytokines in the innate immune system. Their activity is implicated in the regulation of microglial function both during development and in res...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-017-2307-8
更新日期:2017-09-01 00:00:00
abstract::We have utilized quantitative autoradiography with the GABA(A) receptor chloride channel blocker [35S]t-butylbicyclophosphorothionate ([35S]TBPS) in rodent brain sections to investigate if differential proton modulation of various GABA(A) receptor subtypes expressed in various brain regions are differentially sensitiv...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000018849.54169.c4
更新日期:2004-04-01 00:00:00
abstract::The concentrations of taurine in a number of brain regions and in other tissues of rhesus monkeys fed a taurine-free human infant formula for 3 months are substantially lower than in similar monkeys fed the same formula supplemented with taurine. Activities of enzymes involved in taurine biosynthesis were not differen...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00972479
更新日期:1988-04-01 00:00:00
abstract::Systemic inflammation is present in obesity and emerging evidence, primarily from studies using male rodents fed high-fat diets, suggests neuroimmune signaling also is involved. We investigated early changes in neuroimmune signaling during the weight gain that follows ovariectomy in rats. Ovariectomized (OVX) rats wer...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2596-6
更新日期:2018-09-01 00:00:00