Abstract:
:We report further analysis of axonally transported proteoglycans in soluble and membranous subfractions of goldfish optic tectum. Distribution of transported 35SO4 radioactivity was 35.2% soluble, 63.4% Triton-NaCl extractable and 1.4% unextracted. Proteoglycans isolated on DEAE cellulose were treated with chondroitinase AC or nitrous acid and remaining heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans (CSPGs) were sized on Sepharose CL-6B. Kav values and estimated molecular weights were: Soluble CSPG-0.36 (160 kDa), Triton-NaCl extracted CSPG-.031 (200 kDa), Soluble HSPG-0.37 (150 kDa), Triton-NaCl extracted HSPG-0.37 (150 kDa). For constituent CS and HS chains the Kav values and estimated molecular weights on CL-6B were: Soluble CS-0.55 (15 kDa), Triton-NaCl extracted CS-0.55 (15 kDa), Soluble HS-0.59 (13 kDa) and Triton-NaCl extracted HS-0.65 (9 kDa). CS was shown to be sulfated exclusively at carbon 4 for both soluble and Triton NaCl extracted fractions.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Elam JSdoi
10.1007/BF00970543subject
Has Abstractpub_date
1995-02-01 00:00:00pages
187-93issue
2eissn
0364-3190issn
1573-6903journal_volume
20pub_type
杂志文章abstract::Previous studies suggest that alterations of brain glutamate synthesis and release occur in experimental thiamine deficiency. In order to assess the integrity of post-synaptic glutamatergic receptors in thiamine deficiency, binding sites for [3H]glutamate (displaced by NMDA), [3H]-kainate, and [3H]quisqualate (AMPA si...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00995378
更新日期:1995-10-01 00:00:00
abstract::It is known that the NMDA-R NR1 subunit is needed for the receptor activity and that under hypoxia the evolution toward apoptosis or neuronal survival depends on the balance NR2A/NR2B subunits. This paper analyzes the effect of acute hypoxia on the above mentioned subunits mRNAs during development. The mean percentage...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0221-4
更新日期:2010-10-01 00:00:00
abstract::Several studies showed that the up-regulation of glial glutamate transporter-1 (GLT-1) participates in the acquisition of brain ischemic tolerance induced by cerebral ischemic preconditioning or ceftriaxone pretreatment in rats. To explore whether GLT-1 plays a role in the acquisition of brain ischemic tolerance induc...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0639-3
更新日期:2012-03-01 00:00:00
abstract::With the aid of histochemical and electrophoretic techniques activities for esterase and esterprotease were investigated in the digastric and masseter muscles from normal and dystrophic mice. The substrates used were alpha-naphthyl acetate and N-acetyl-L-alanine alpha-naphthyl ester. According to the microscopic obser...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971312
更新日期:1989-03-01 00:00:00
abstract::Effects of single and repeated doses of acrylamide on fast and slow axonal transport of radio labeled proteins following the injection of L-[4,5-3H] leucine have been studied in the optic system of male Sprague-Dawley rats. A single dose of acrylamide (100 mg/kg) had no effect, but higher concentrations (200-300 mg/kg...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973750
更新日期:1990-06-01 00:00:00
abstract::gamma-L-Glutamylglutamate (LGG), an endogenous constituent of the brain, reduced the glutamate-evoked increase in intracellular Ca2+ in cultured cerebellar granule cells. The extent and properties of this inhibition were different at different Mg2+ concentrations. The intracellular Ca2+ response to NMDA was slightly e...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970596
更新日期:1995-12-01 00:00:00
abstract::Ellagic acid (EA, 2,3,7,8-tetrahydroxy-chromeno; C14H6O8) is a polyphenol derived from fruits (pomegranates, berries) and nuts. EA exhibits antioxidant capacity and induces anti-inflammatory actions in several mammalian tissues. EA has been characterized as a possible neuroprotective agent, but the number of reports i...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-016-1853-9
更新日期:2016-06-01 00:00:00
abstract::Focal epileptic seizures can in some patients be managed by inhibiting γ-aminobutyric acid (GABA) uptake via the GABA transporter 1 (GAT1) using tiagabine (Gabitril®). Synergistic anti-seizure effects achieved by inhibition of both GAT1 and the betaine/GABA transporter (BGT1) by tiagabine and EF1502, compared to tiaga...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03017-y
更新日期:2020-07-01 00:00:00
abstract::Taurine is a semiessential amino acid that is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, and leukocytes. Taurine reaches up to 50 mM concentration in leukocytes. Taurine has been shown to be tissue-protecti...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/b:nere.0000010440.37629.17
更新日期:2004-01-01 00:00:00
abstract::Thiamine deficiency (TD) produces severe neurodegenerative lesions. Studies have suggested that primary neurodegenerative events are associated with both oxidative stress and inflammation. Very little is known about the downstream effects on intracellular signaling pathways involved in neuronal death. The primary aim ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-02975-7
更新日期:2020-04-01 00:00:00
abstract::We examined the impact of peroxiredoxin-I (Prx-I) and peroxiredoxin-II (Prx-II) stable transduction on oxidative stress in PC12 neurons and NIH3T3 fibroblasts and found variability depending on cell type and Prx subtype. In PC12 neurons, Prx-II suppressed reactive oxygen species (ROS) generation by 36% (p < 0.01) rela...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1026670620633
更新日期:2000-12-01 00:00:00
abstract::Deprenyl has been discovered by Knoll and co-workers. The R-enantiomer of deprenyl (selegiline) is a selective and irreversible inhibitor of the B-isoform of monoamine oxidase (MAO-B) enzyme. Due to its dopamine potentiating and possible neuroprotective properties it has an established role in the treatment of parkins...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0238-8
更新日期:2010-12-01 00:00:00
abstract::Human amniotic membrane mesenchymal stem cells (hAMSCs) are considered ideal candidate stem cells for cell-based therapy. In this study, we assessed whether hAMSCs transplantation promotes neurological functional recovery in rats after traumatic spinal cord injury (SCI). In addition, the potential mechanisms underlyin...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1987-9
更新日期:2016-10-01 00:00:00
abstract::Acetate is a two carbon intermediate in metabolism. It is an accepted marker of astrocytic metabolism, and a substrate for production of metabolites such as glutamine, glutamate and GABA. However, anomalies exist in the current explanations of compartmentation and metabolism of acetate. Here, we investigated these ano...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-012-0847-5
更新日期:2012-11-01 00:00:00
abstract::Olfactory ensheathing cells (OECs) and Schwann cells (SCs) are closely-related cell types with regeneration-promoting properties. Comparative gene expression analysis is particularly relevant since it may explain cell type-specific effects and guide the use of each cell type into special clinical applications. In the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1006-3
更新日期:2013-05-01 00:00:00
abstract::Postnatal developmental patterns of activity for acid and alkaline deoxyribonuclease (DNase) were determined in the cerebellum of the androgenized female rat. Littermates were injected s.c. with either 1.25 mg testosterone propionate (TP) or vehicle 48 hours after birth. The enzymes and DNA content were measured in th...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964659
更新日期:1985-04-01 00:00:00
abstract::Aliskiren (ALK), a pharmacological renin inhibitor, is an effective antihypertensive drug and has potent anti-apoptotic activity, but it is currently unknown whether ALK is able to attenuate brain damage caused by acute cerebral ischemia independent of its blood pressure-lowering effects. This study aimed to investiga...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1944-7
更新日期:2016-09-01 00:00:00
abstract::Kainic acid-induced seizures produced early (2 hr) generalized edema and later (24 and 48 hr) necrotic edema in temporal cortex and hippocampus as measured by specific gravity changes. Mannitol given during the seizure partially protected against the early edema and prevented the necrotic edema indicating early edema ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01000241
更新日期:1987-06-01 00:00:00
abstract::A number of recent findings support the notion of mechanistic parallels between Alzheimer disease (AD) and oncogenic processes, specifically, that neurons in AD, like cancer cells, display aberrant mitotic cell cycle re-entry. However, the mechanism that drives postmitotic neurons to reenter cell cycle remains elusive...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9146-3
更新日期:2007-04-01 00:00:00
abstract::Sex differences exist in the functioning of the two brain corticosteroid receptor systems. Ovarian steroid replacement alters receptor mRNA expression, receptor binding capacities, and receptor affinity. The abundance of both mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) message can be reduced by es...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1022023207326
更新日期:1997-11-01 00:00:00
abstract::We studied the effects of transcranial magnetic stimulation (TMS, 60 Hz and 0.7 mT for 4 h/day for 14 days) on oxidative and cell damage caused by olfactory bulbectomy (OBX) in Wistar rats. The levels of lipid peroxidation products and caspase-3 were enhanced by OBX, whereas it prompted a reduction in reduced glutathi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0172-9
更新日期:2010-08-01 00:00:00
abstract::The primary auditory cortex is subject to the modulation of numerous neurotransmitters including norepinephrine (NE), which has been shown to decrease cellular excitability by yet unclear mechanisms. We investigated the possibility that NE directly affects excitatory glutamatergic synapses. We found that bath applicat...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-9966-z
更新日期:2009-11-01 00:00:00
abstract::The SAM strains, a group of related inbred strains consisting of senescence-prone inbred strains (SAMP) and senescence-resistant inbred strains (SAMR), have been successfully developed by selective inbreeding of the AKR/J strain of mice donated by the Jackson laboratory in 1968. The characteristic feature of aging com...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-009-9922-y
更新日期:2009-04-01 00:00:00
abstract::Dual-specificity tyrosine-regulated kinase 2 (DYRK2), a protein kinase that phosphorylates its substrates on serine/threonine, is expressed in numerous human tumors, but little is known about its role in the pathophysiology of glioma. In this study, we made an effort to explore the expression and function in human gli...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2345-2
更新日期:2017-11-01 00:00:00
abstract::The present work describes the kinetics of enkephalin hydrolysis by plasma enzymes and the fragmentation pattern of both the parent peptide and of the first hydrolysis by-products. The degradation kinetics were followed by positive identification of the hydrolysis fragments by chromatographic methods, by amino acid an...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964980
更新日期:1985-10-01 00:00:00
abstract::Regional and developmental changes in metabolite concentrations were measured by 1H NMR spectroscopy and HPLC of perchloric acid extracts from rat brain and eye. The highest concentrations of N-acetylaspartate were found in grey matter as opposed to white matter with concentration increasing with age from neonate to a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02532417
更新日期:1996-09-01 00:00:00
abstract::We review here the possible mechanisms of neuronal degeneration caused by L-cysteine, an odd excitotoxin. L-Cysteine lacks the omega carboxyl group required for excitotoxic actions via excitatory amino acid receptors, yet it evokes N-methyl-D-aspartate (NMDA) -like excitotoxic neuronal death and potentiates the Ca2+ i...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1007616817499
更新日期:2000-10-01 00:00:00
abstract::The selective vulnerability of specific neuronal subpopulations to trimethyltin (TMT), an organotin compound with neurotoxicant effects selectively involving the limbic system and especially marked in the hippocampus, makes it useful to obtain in vivo models of neurodegeneration associated with behavioural alterations...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-012-0932-9
更新日期:2013-02-01 00:00:00
abstract::The effect of optic nerve transsection on proteolytic degradation of axonally transported proteins in the superior colliculus of the rabbit was studied. Proteolysis of labeled proteins was determined in vitro in small pieces of the superior colliculus. Within 2 hours after sectioning the optic nerve there was a decrea...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973280
更新日期:1988-07-01 00:00:00
abstract::We found that chronic lithium diet affects the sensitivity of neuroleptic receptors and the content of amino acids in the brain, and that the changes in adult animals differ from those in young rats. Pregnant rats were kept on lithium diet (pellets with 0.21% Li2CO3 and 0.21% NaCl) during the gestation period and the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964703
更新日期:1983-05-01 00:00:00