Abstract:
:With the aid of histochemical and electrophoretic techniques activities for esterase and esterprotease were investigated in the digastric and masseter muscles from normal and dystrophic mice. The substrates used were alpha-naphthyl acetate and N-acetyl-L-alanine alpha-naphthyl ester. According to the microscopic observations of the dystrophic muscles the histopathological changes in the masseter muscle were much more pronounced than in the digastric muscle. The connective tissue surrounding the myofibers of the dystrophic masseter contained a large number of cells with pronounced enzyme activity. Among them were mast cells that were strongly stained for esterprotease. The connective tissue of the dystrophic digastricus was much less infiltrated with cellular elements reacting for esterprotease. In zymograms the normal digastricus, the dystrophic masseter and the dystrophic digastricus showed a strong activity for certain isoenzymes that were absent or weakly expressed in the normal masseter.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Kirkeby S,Moe D,Vilmann Hdoi
10.1007/BF00971312subject
Has Abstractpub_date
1989-03-01 00:00:00pages
211-5issue
3eissn
0364-3190issn
1573-6903journal_volume
14pub_type
杂志文章abstract::We investigated the ability of hydrogen peroxide (H2O2) to cause apoptotic cell death in cultured rat forebrain neurons and the potential mechanisms by which oxidative stress triggers delayed neuronal death. H2O2 (25 microM for 5 min) reduced cell viability to 34.5 +/- 8.3% of untreated controls 20 h after exposure, a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022403224901
更新日期:1997-03-01 00:00:00
abstract::Oxidative stress is one of the most important pathological mechanisms in neurodegenerative diseases and ischemia. Recent studies have indicated that the sonic hedgehog (SHH) signaling pathway is involved in these diseases, but the underlying mechanisms remains elusive. Here we report that the SHH pathway was activated...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0264-6
更新日期:2011-01-01 00:00:00
abstract::Age-related increase of reactive oxygen species (ROS) is particularly detrimental in postmitotic tissues. Calorie restriction (CR) has been shown to exert beneficial effects, consistent with reduced ROS generation by mitochondria. Many antioxidant compounds also mimic such effects. N-acetyl cysteine (NAC) provides thi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-6867-7
更新日期:2005-06-01 00:00:00
abstract::In vitro aggregation and fibrillization of synthetic amyloid beta-protein Abeta 1-40 was assessed in the conditioned media from rhabdomyosarcoma (CRL 1598, HTB 82, HTB 153, CCL 136), adenocarcinoma (CCL 218), neuroblastoma (SY5Y), and COS cells cultured in the absence and presence of 10% heat-inactivated fetal bovine ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027379926976
更新日期:1997-02-01 00:00:00
abstract::The effect of isoproterenol, norepinephrine, dopamine, and serotonin on the accumulation of adenosine 3',5'-monophosphate (cAMP) was studied in homogeneous neuronal cultures from 8-day chick embryo hemispheres. Among the catecholamines, isoproterenol had a more pronounced effect on the accumulation of cAMP. Norepineph...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966167
更新日期:1980-10-01 00:00:00
abstract::One pathway by which tryptophan is metabolized in the brain as well as in the periphery is through cleavage of the indole ring to formylkynurenine and then kynurenine. Indoleamine-2,3-dioxygenase, the enzyme that catalyzes this cleavage, and kynurenine are distributed all across the different anatomic regions of brain...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00964611
更新日期:1980-03-01 00:00:00
abstract::The binding of the enkephalin dimer [D-Ala2, Leu5-NH-CH2-]2 (DPE2) is characterized by its high affinity for receptors on NG108-15 hybrid cells, the affinity constant K = 4.7 X 10(9) M-1 is up to 8-fold that of monomers (0.6 to 1.0 X 10(9) M-1), and a maximal binding capacity equal to one half that of the monomers. Ki...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965208
更新日期:1986-06-01 00:00:00
abstract::Social isolation during early development is one of the most potent stressors that can cause alterations in the processes of brain maturation, leading to behavioral and neurochemical changes that may persist to adulthood. Exposure to palatable diets during development can also affect neural circuits with long-term con...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1083-3
更新日期:2013-09-01 00:00:00
abstract::Using the replication-defective adenovirus vector, we overexpressed rat glia maturation factor (GMF) in primary astrocyte cultures derived from embryonic rat brains. Among the three isoforms of MAP kinase, there was a big increase in the phosphorylation of p38, as detected with Western blotting using the phosphospecif...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1014241300179
更新日期:2001-12-01 00:00:00
abstract::Radioactive azide derivatives of tetrodotoxin (TTX) were synthesized using 2-nitro-4-azidephenyl-[3H]beta alanine for the purpose of photolabeling of the Na channel. Three azide derivatives, N1, N2 and N3, were separated by ion exchange chromatography on Bio-Rex 70 resin and reversed phase high performance liquid chro...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965886
更新日期:1985-08-01 00:00:00
abstract::We found that chronic lithium diet affects the sensitivity of neuroleptic receptors and the content of amino acids in the brain, and that the changes in adult animals differ from those in young rats. Pregnant rats were kept on lithium diet (pellets with 0.21% Li2CO3 and 0.21% NaCl) during the gestation period and the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964703
更新日期:1983-05-01 00:00:00
abstract::Most inborn errors of tyrosine catabolism produce hypertyrosinemia. Neurological manifestations are variable and some patients are developmentally normal, while others show different degrees of developmental retardation. Considering that current data do not eliminate the possibility that elevated levels of tyrosine an...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1078-0
更新日期:2013-08-01 00:00:00
abstract::We identify and characterize two classes of immediate-early genes: (i) genes, induced by depolarization in neurons, that play a role in depolarization-induced neuronal plasticity and (ii) genes, induced in neuronal precursors by neurotrophins, that play a causal role in neurotrophin-directed neuronal differentiation. ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1007546600535
更新日期:2000-05-01 00:00:00
abstract::Rabbits were immunized with the myelin-associated glycoprotein (MAG) that had been purified from isolated rat brain myelin by selective extraction with lithium diiodosalicylate (LIS) and phenol followed by preparative SDS gel electrophoresis. Antibodies to MAG were detected qualitatively by immunodiffusion and quantit...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964417
更新日期:1981-10-01 00:00:00
abstract::Schwann cell biosynthesis of glycolipids was studied by in vitro incorporation of [3H]galactose into neonatal rat sciatic nerves before and after endoneurial explant culture and in culture of purified Schwann cells. In neonatal nerves prior to culture, [3H]galactose was actively incorporated into galactocerebrosides (...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968672
更新日期:1990-03-01 00:00:00
abstract::Bilateral injection of 6-hydroxydopamine into the medial forebrain bundle (MFB) significantly decreased monoamine concentrations in the hypothalamus. The noradrenaline and serotonin content of the paraventricular nucleus (PVN) was also significantly reduced. These drastic decreases in neurotransmitter concentration di...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01474679
更新日期:1993-02-01 00:00:00
abstract::The axonal transport of tRNA has been investigated in the chick optic system. Chicks were injected with [3H]uridine intraocularly or intracranially and the RNA of the retina, nerve complex, and tecta separated by polyacrylamide gel electrophoresis and then counted. The ratio of tRNA to rRNA specific activities increas...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966323
更新日期:1978-08-01 00:00:00
abstract::Neuroprotectin D1 (NPD1), a docosahexaenoic acid (DHA)-derived lipid mediator, promotes survival in cells exposed to oxidative stress by inducing the activity of anti-inflammatory mediators and suppressing the expression of pro-inflammatory genes. Though retinal pigment epithelial (RPE) cells naturally produce NPD1 fr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0351-8
更新日期:2010-12-01 00:00:00
abstract::Neuronal excitation leads to an increase of the extracellular K(+) concentration ([K(+)]o) in brain. This increase has at least two energy-consuming consequences: (1) a depolarization-mediated change in intracellular pH (pHi) in astrocytes due to depolarization-mediated increased activity of the acid-extruding Na(+)/b...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1149-2
更新日期:2013-11-01 00:00:00
abstract::The Wistar-Kyoto (WKY) rat strain has been described as an animal model of depressive behavior that consumes significantly greater amounts of alcohol compared to the Wistar (WIS) rat strain. Since the mesolimbic dopamine (DA) type-2 (D2) receptors mediate reward-related behaviors, the present study measured the bindin...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0233-0
更新日期:2010-11-01 00:00:00
abstract::There is evidence that many of the GABA(A) receptor subunits contain consensus sequence for tyrosine kinase, and phosphorylation may play a key role in ethanol's regulation of GABA(A) receptors. Recently, we investigated the effect of chronic exposure of ethanol (CE) on tyrosine kinase phosphorylation and reported tha...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9124-9
更新日期:2006-09-01 00:00:00
abstract::Brief limb ischemia was reported to protect neurons against injury induced by subsequent cerebral ischemia-reperfusion, and this phenomenon is known as limb ischemic preconditioning (LIP). To explore the role of nitric oxide (NO) in neuroprotection of LIP in rats, we observed changes in the content of nitric oxide (NO...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9381-2
更新日期:2007-11-01 00:00:00
abstract::Parkinson's disease (PD) is characterized by the presence of insoluble protein clusters containing α-synuclein. Impairment of mitochondria, endoplasmic reticulum, autophagy and intracellular trafficking proper function has been suggested to be caused by α-synuclein toxicity, which is also associated with the higher le...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-018-2673-x
更新日期:2018-12-01 00:00:00
abstract::Bovine brain synaptic membranes which were frozen and then extensively washed showed low affinity [3H]muscimol binding. These membranes contained GABA and calmodulin, apparently tightly bound within the membrane fraction. Membranes which were additionally treated with the detergent Triton X-100 showed high affinity [3...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970756
更新日期:1988-09-01 00:00:00
abstract::Some crustacean axons remain functional for months after injury. This unusual property may require stress proteins synthesized by those neurons or provided to them by glial cells. To begin to explore this hypothesis, we examined the conditions that stimulated stress protein synthesis by crayfish CNS tissue in vitro. I...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00974871
更新日期:1991-05-01 00:00:00
abstract::A simple, rapid method for determining depolarization-induced 45Ca influx into synaptosomes is described. Synaptosomes which had been depolarized in the presence of 45Ca were applied to a small column of Chelex-100 resin to separate free Ca2+ from that taken up by the tissue. Approximately 70% of the synaptosomal prot...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965062
更新日期:1982-02-01 00:00:00
abstract::Phosphate activated glutaminase (PAG) was assayed in whole homogenate and synaptosomes of cerebral cortex from normal or fasted for 120 h rats. The specific activity (s.a.) of PAG was found diminished by 25% in the whole homogenate from the fasted animals compared to the normal. On the contrary, fasting did not affect...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965830
更新日期:1991-01-01 00:00:00
abstract::The concept of exosomes has been progressively changed from the status of cellular trashcans to multitasking organelles involved in many processes, including internalization, transport and transfer of macromolecules such as proteins, lipids and nucleic acids. While underpinning the mechanisms behind neurodegeneration ...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-020-03111-1
更新日期:2020-11-01 00:00:00
abstract::Biotinylated derivatives of tetanus toxin were prepared and isolated by chromatofocusing and ganglioside-affinity chromatography. Biotinylation was monitored by the appearance of a 210,00 dalton complex upon SDS-polyacrylamide gel electrophoresis in the presence of avidin, and by selective binding to an avidin-Sepharo...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969922
更新日期:1990-04-01 00:00:00
abstract::Overactivation of glutamate receptors results in neurodegeneration in a variety of brain pathologies, including ischemia, epilepsy, traumatic brain injury and slow-progressing neurodegenerative disorders. In all these pathologies, it is well accepted that the calcium-dependent cysteine proteases calpains are key playe...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0323-z
更新日期:2010-12-01 00:00:00