Abstract:
:Rabbits were immunized with the myelin-associated glycoprotein (MAG) that had been purified from isolated rat brain myelin by selective extraction with lithium diiodosalicylate (LIS) and phenol followed by preparative SDS gel electrophoresis. Antibodies to MAG were detected qualitatively by immunodiffusion and quantitatively by a double antibody assay utilizing [3H]fucose-labeled MAG as antigen. The antisera were capable of precipitating between 300 and 500 micrograms of MAG/ml of serum under the conditions of the assay. Preincubation of the anti-MAG serum with other glycoproteins or glycolipids did not inhibit the precipitation of labeled MAG. Similarly, preincubation of the antiserum with LIS-phenol extracts of non-neural tissues did not inhibit the immune precipitation of MAG. The specificity of the antiserum was also indicated by the selective double antibody precipitation of MAG from solubilized whole myelin that contained a heterogeneous mixture of [3H]fucose-labeled glycoproteins. The antibodies to MAG were not effectively absorbed by whole brain homogenate or purified myelin, indicating that the antigenic site(s) is not accessible in the intact membranes, but can be exposed by treatment with detergent or partial purification. Low levels of antibodies reacting with MAG were detected in three rabbits with experimental allergic encephalomyelitis induced by injection of purified myelin in complete Freund's adjuvant.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Quarles RH,Johnson D,Brady RO,Sternberger NHdoi
10.1007/BF00964417subject
Has Abstractpub_date
1981-10-01 00:00:00pages
1115-27issue
10eissn
0364-3190issn
1573-6903journal_volume
6pub_type
杂志文章abstract::Exposure of guinea pig brain slices to low concentrations (10 microM) of NMDA caused decreases in PCr and ATP within 30 min, with a slower decrease in NAA and increase in lactate, both detectable after 1 h. Exposure to NMDA for over 1 h or at higher concentrations caused further increases in lactate and decreases in N...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1014898421330
更新日期:2002-02-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00
abstract::The inflammatory response mediated by microglia in the central nervous system is closely related to epilepsy. Notch signaling plays an important role in the microglial activation during hypoxia. This study aimed to investigate whether Notch signaling is involved in microglial activation and subsequent inflammation-rel...
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pub_type: 杂志文章
doi:10.1007/s11064-018-2544-5
更新日期:2018-06-01 00:00:00
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journal_title:Neurochemical research
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更新日期:2018-02-01 00:00:00
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journal_title:Neurochemical research
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journal_title:Neurochemical research
pub_type: 杂志文章,评审
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更新日期:2007-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-9966-z
更新日期:2009-11-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966692
更新日期:1993-10-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1020772706279
更新日期:1999-09-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1341-z
更新日期:2014-08-01 00:00:00
abstract::Intrauterine growth restricted (IUGR) infants are at increased risk for neurodevelopmental deficits that suggest the hippocampus and cerebral cortex may be particularly vulnerable. Evaluate regional neurochemical profiles in IUGR and normally grown (NG) 7-day old rat pups using in vivo 1H magnetic resonance (MR) spect...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1609-y
更新日期:2017-01-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-9929-4
更新日期:2009-08-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-9008-4
更新日期:2006-02-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964783
更新日期:1980-06-01 00:00:00
abstract::(4S)-4-Amino-5,6-heptadienoic acid [S)-gamma-allenyl-GABA; MDL 72483) is a potent inactivator of brain GABA-T in mice; (ED50 (i.p.) = 60 mg.kg-1; ED50 (oral) = 70 mg.kg-1). Its anticonvulsant effects against 3-mercaptopropionic acid (MPA)-induced seizures in mice is related to the elevation of whole brain GABA concent...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966092
更新日期:1991-03-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963906
更新日期:1981-01-01 00:00:00
abstract::Brief limb ischemia was reported to protect neurons against injury induced by subsequent cerebral ischemia-reperfusion, and this phenomenon is known as limb ischemic preconditioning (LIP). To explore the role of nitric oxide (NO) in neuroprotection of LIP in rats, we observed changes in the content of nitric oxide (NO...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9381-2
更新日期:2007-11-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2445-z
更新日期:2018-02-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9629-5
更新日期:2008-09-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9299-8
更新日期:2007-07-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022482520358
更新日期:1997-03-01 00:00:00
abstract::Analysis of gene expression in the brain is a valuable tool to study the function of the brain under normal and pathological conditions. Although there are many techniques used to measure gene expression the validity of any such experiment is directly related to the quality of the RNA in the samples. The most readily ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020996519419
更新日期:2002-10-01 00:00:00
abstract::Previous work from this laboratory and others has shown that neurotransmitters can activate phospholipase D. Unlike the phospholipase C that specifically hydrolyzes inositol-containing phospholipids, phospholipase D in neuronal tissue specifically hydrolyzes phosphatidylcholine. One route for the synthesis of phosphat...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020716304520
更新日期:1998-08-01 00:00:00
abstract::The release of the inhibitory neurotransmitter GABA is generally enhanced under potentially cell-damaging conditions. The properties and regulation of preloaded [3H]GABA release from mouse hippocampal slices were now studied in free radical-containing medium in a superfusion system. Free radical production was induced...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9439-1
更新日期:2008-03-01 00:00:00
abstract::During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering multiple cellular responses to injury. Here, we evaluated changes in extracellular ATP, ADP and AMP hydrolysis at different times (0-24 hours) after unilateral cortical stab injury (CSI) in adul...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9529-0
更新日期:2008-05-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03166-0
更新日期:2020-11-12 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965906
更新日期:1990-09-01 00:00:00
abstract::Passive avoidance (PA) conditioning is a fear motivated task able to initiate a cascade of altered gene expression within the hippocampus, a structure critical to learning and memory. We have previously shown that neurofibromin (NF1) and amyloid precursor protein (APP), two genes implicated in cognitive function, are ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0949-0
更新日期:2013-03-01 00:00:00
abstract::Taurine is a semiessential amino acid that is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, and leukocytes. Taurine reaches up to 50 mM concentration in leukocytes. Taurine has been shown to be tissue-protecti...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/b:nere.0000010440.37629.17
更新日期:2004-01-01 00:00:00