Abstract:
:Detergents Triton X-100, sodium deoxycholate, and octyl-beta-D-glucopyranoside, and proteinase papain proved to be excellent agents solubilizing the gamma-glutamyltransferase (gamma-GT) from human brain cortex microvessels. Ficin also solubilized gamma-GT but to a lesser extent than papain. The relative molecular mass of the detergent-solubilized enzyme form was greater than 200,000 (in the presence of Triton X-100). The relative molecular mass of the proteinase-solubilized form was slightly greater than that of albumine. gamma-GTs of microvessels from five human brain regions and from the choroid plexus were tested for their specificity toward acceptors. The best acceptors were found to be (in decreasing order of activity) L-cystine, glycylglycine, L-glutamine, L-methionine, and L-alanine. The findings suggest that the main features of gamma-GT of the human blood-brain barrier are very similar to those of gamma-GTs from other human tissues.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Veselý J,Cernoch Mdoi
10.1007/BF00964524subject
Has Abstractpub_date
1984-07-01 00:00:00pages
927-34issue
7eissn
0364-3190issn
1573-6903journal_volume
9pub_type
杂志文章abstract::We tested the action of proline-rich peptide (PRP-1) and cobra venom Naja Naja Oxiana (NOX) on Deiters' nucleus neurons at 3rd, 15th and 35th days after unilateral labyrinthectomy (UL). Early and late tetanic, post-tetanic potentiation and depression of Deiters'neurons to bilateral high frequency stimulation of hypoth...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0239-7
更新日期:2010-11-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:1979-06-01 00:00:00
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更新日期:2020-11-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章,评审
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:1996-03-01 00:00:00
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doi:10.1007/s11064-010-0327-8
更新日期:2011-02-01 00:00:00
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doi:10.1007/s11064-013-0985-4
更新日期:2013-04-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03156-2
更新日期:2020-10-27 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966702
更新日期:1991-11-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-008-9694-9
更新日期:2008-12-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9895-2
更新日期:2009-07-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1484-y
更新日期:2015-03-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964157
更新日期:1983-12-01 00:00:00
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更新日期:2016-12-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969089
更新日期:1993-03-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01705542
更新日期:1995-06-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970759
更新日期:1988-09-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:1978-02-01 00:00:00
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更新日期:2009-05-01 00:00:00
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更新日期:2019-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1023/a:1027320113151
更新日期:1997-04-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965657
更新日期:1983-01-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2366-x
更新日期:2017-11-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020984114824
更新日期:1999-01-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965201
更新日期:1983-08-01 00:00:00
abstract::Chemotherapy drugs such as vincristine (VCR) can cause neuropathic pain, and there is still lack of ideal strategy to treat it. The current study was designed to investigate effect of matrine (MT) on VCR-induced neuropathic pain in animal model. VCR (75 μg/kg, i.p. for 10 consecutive days) was administered to induce p...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2040-8
更新日期:2016-11-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964144
更新日期:1979-04-01 00:00:00