Abstract：
:Nitric oxide has been strongly implicated in the development of tissue infarction in response to focal cerebral ischemia. Nitric oxide and its derivatives can inhibit components of the electron transport chain, providing a likely target for these substances in ischemic and post-ischemic brain. Lactate content is increased during post-ischemic reperfusion in tissue destined to become infarcted, consistent with impairment of mitochondrial respiration. To investigate the possible involvement of nitric oxide in generating these changes, we have tested the effect of 7-nitroindazole, a nitric oxide synthase (NOS) inhibitor, on the content of lactate and other metabolites during early reperfusion following temporary focal ischemia. This treatment inhibited total NOS by approximately 50%. However, the treatment did not significantly affect the marked increases in lactate in post-ischemic brain nor did it alter the recovery of other energy-related metabolites. These findings indicate that inhibition of oxidative metabolism is probably not the primary site of the deleterious effects of nitric oxide and derivatives during early post-ischemic reperfusion.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Helps SC,Sims NRdoi
10.1007/s11064-006-9160-5subject
Has Abstractpub_date
2007-04-01 00:00:00pages
663-70issue
4-5eissn
0364-3190issn
1573-6903journal_volume
32pub_type
杂志文章abstract：:We studied the effects of transcranial magnetic stimulation (TMS, 60 Hz and 0.7 mT for 4 h/day for 14 days) on oxidative and cell damage caused by olfactory bulbectomy (OBX) in Wistar rats. The levels of lipid peroxidation products and caspase-3 were enhanced by OBX, whereas it prompted a reduction in reduced glutathi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0172-9
更新日期：2010-08-01 00:00:00
abstract：:Amyotrophic lateral sclerosis (ALS) is a degenerative disorder caused by motor neuron loss. T-cell intracellular antigen-1 (TIA-1), a cytotoxic T lymphocyte granule-associated RNA binding protein, is a key component of stress granules. However, it remains uncertain whether ALS-causing superoxide dismutase-1 (SOD1) tox...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03137-5
更新日期：2020-12-01 00:00:00
abstract：:The decline in neuronal function during aging may result from increases in extracellular glutamate (Glu), Glu-induced neurotoxicity, and altered mitochondrial metabolism. To study metabolic responses to persistently high levels of Glu at synapses during aging, we used transgenic (Tg) mice that over-express the enzyme ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1239-9
更新日期：2014-01-01 00:00:00
abstract：:The activity, properties, and developmental pattern of cysteine sulfinate transaminase (CSA-T) were studied in chick retina and compared with the activity, properties, and developmental pattern of glutamate oxaloacetate transaminase (GOT). Their optimum pH is identical whereas the effect of pyridoxal phosphate seems t...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964358
更新日期：1978-02-01 00:00:00
abstract：:This study evaluates whether physiological variables differentially affect the local synthesis of protein constituents of synapses in subcellular fractions containing pinched-off dendrites (synaptodendrosomes). Synaptodendrosomes were pulse-labeled in a medium containing 35S-methionine with 3 or 25 mM KCl and in the p...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02527725
更新日期：1996-06-01 00:00:00
abstract：:The effect of ageing and the relationships between the catalytic properties of enzymes linked to Krebs' cycle, electron transfer chain, glutamate and aminoacid metabolism of cerebral cortex, a functional area very sensitive to both age and ischemia, were studied on mitochondria of adult and aged rats, after complete i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0004-y
更新日期：2009-12-01 00:00:00
abstract：:CNS trauma has been associated with an increase in free radical production, but the cellular sources of this increase or the mechanism involved in the production of free radicals are not known. We, therefore, investigated the effects of trauma on free radical production in cultured neurons, astrocytes and BV-2 microgl...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1014875210852
更新日期：2002-02-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common type of neurodegenerative dementia that affects the elderly population. Nerve growth factor (NGF) contributes to the survival, regeneration and death of neurons during aging and in neurodegenerative diseases. Recently, research has shown that NGF is related to the pathology,...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-016-1829-9
更新日期：2016-06-01 00:00:00
abstract：:Calcium-naive synaptosomes were used to assess the effects of divalent cations on [3H]acetylcholine release from rat hippocampal homogenates. Following equilibration with calcium-free buffer (containing 10 microM EGTA), calcium reversibly increased [3H]acetylcholine efflux (up to five-fold) while causing no measurable...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966598
更新日期：1991-10-01 00:00:00
abstract：:In this study, we observed the effects of metformin, one of the most widely prescribed drugs for the treatment of type 2 diabetes, on cell proliferation and neuroblast differentiation in the subgranular zone of the hippocampal dentate gyrus (SZDG) in Zucker diabetic fatty (ZDF) rats, which are a model for type 2 diabe...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0115-5
更新日期：2010-04-01 00:00:00
abstract：:Overactivation of glutamate receptors results in neurodegeneration in a variety of brain pathologies, including ischemia, epilepsy, traumatic brain injury and slow-progressing neurodegenerative disorders. In all these pathologies, it is well accepted that the calcium-dependent cysteine proteases calpains are key playe...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0323-z
更新日期：2010-12-01 00:00:00
abstract：:Mitochondrially bound brain hexokinase is solubilized by anesthetics and this effect has been suggested to contribute to anesthesia. In the present investigation the influence of the metabolic inhibitor 2-deoxy-D-glucose (2-DOG) was studied. An isolated rat brain preparation was used to avoid the contribution of perip...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965526
更新日期：1982-06-01 00:00:00
abstract：:There is growing evidence that ketone bodies (KB)-derived from fatty acid oxidation and produced during fasting or consumption of high-fat diets-can exert broad neuroprotective effects. With respect to epilepsy, KB (such as β-hydroxybutyrate or BHB, acetoacetate and acetone) have been shown to block acutely induced an...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2253-5
更新日期：2017-07-01 00:00:00
abstract：:Exposure to Hg2+ below 10 micrometer destroys synaptosomal membrane-associated sialidase of bovine brain in situ. Inhibition by Cu2+ occurs only at relatively higher concentrations, and is demonstrable after the synaptosomal membrane preparation has been presaturated with Cu2+ . Pb2+ does not inhibit enzymatic activit...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964363
更新日期：1978-02-01 00:00:00
abstract：:The interactions of S-nitrosoglutathione (GSNO) with the ionotropic glutamate receptors were studied on synaptic membranes isolated from the pig cerebral cortex. GSNO displaced the binding of [3H]glutamate, 3-[(R)-2-carboxypiperazin-4-yl] [3H]propyl-1-phosphonate ([3H]CPP), a competitive N-methyl-D-aspartate (NMDA) an...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007626230278
更新日期：2000-08-01 00:00:00
abstract：:The pathophysiological mechanism(s) underlying Alzheimer's disease (AD) still remain unclear, and no disease-modifying or prophylactic therapies are currently available. Unraveling the fundamental neuropathogenesis of AD is an important challenge. Several studies on AD have suggested lesions in a number of CNS areas i...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-011-0680-2
更新日期：2012-04-01 00:00:00
abstract：:A very large body of evidence from in vivo studies has been accumulated on a link between the change of energy and cell survival/apoptosis. Using an in vitro ischemia model, we have previously shown that ischemia could induce apoptosis in astrocytes. In this study, we utilized the same in vitro model to investigate ch...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021691112190
更新日期：2002-12-01 00:00:00
abstract：:Ecto-nucleotidases play a pivotal role in terminating the signalling via ATP and in producing adenosine, a neuromodulator in the nervous system. We have now investigated the pattern of adenosine formation with different concentrations of extracellular ATP in rat hippocampal nerve terminals. It was found that adenosine...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1012392719601
更新日期：2001-09-01 00:00:00
abstract：:During the past decade a new approach to pathogenetic studies of hepatic encephalopathy has been undertaken to identify the neurochemical alterations which characterize the syndrome. Using animal models of hepatic encephalopathy electrophysiological, behavioral, pharmacological and biochemical evidence were provided o...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00972205
更新日期：1990-02-01 00:00:00
abstract：:This review summarizes the recent research development on mammalian sialidase molecular cloning. Sialic acid-containing compounds are involved in several physiological processes, and sialidases, as glycohydrolytic enzymes that remove sialic acid residues, play a pivotal role as well. Sialidases hydrolyze the nonreduci...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1020276000901
更新日期：2002-08-01 00:00:00
abstract：:It is well-known that pH changes can influence a lot of cellular processes. In this work, we have specifically studied the influence of alkalinization, which can be developed in spinal cord neurons during hyperventilation (respiratory alkalosis) and chronic renal failure (metabolic alkalosis) on calcium homeostasis. A...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000035800.65411.3e
更新日期：2004-09-01 00:00:00
abstract：:It has been reported that electrical stimulation (ES) can promote nerve regeneration. One of the key factors leading to the promotion is calcium, which plays a vital role in the regulation on electrical activity of neurons, but the detailed mechanism is still an open question. In this study, ES was used to stimulate c...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1197-7
更新日期：2014-01-01 00:00:00
abstract：:Unrelated cells such as dental follicle precursor cells (DFPCs) and retinal Müller cells (MCs) make spheres after cultivation in serum-replacement medium (SRM). Until today, the relation and molecular processes of sphere formation from different cell types remain undescribed. Thus in this study we compared proteomes o...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0524-0
更新日期：2011-11-01 00:00:00
abstract：:We observed that AP-3, an antagonist of metabotropic glutamate receptors, reduced carbachol-induced hydrolysis of phospholipids in hippocampal slices. This inhibition could be explained in different ways, e.g.: 1) AP-3 acts also as antagonist of muscarinic receptors mediating the hydrolysis of phospholipids induced by...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022423415238
更新日期：1998-06-01 00:00:00
abstract：:The developmental profiles of acetylcholinesterase and choline acetyltransferase in chick optic tectum and retina cell aggregates, over a 30-day period, have been determined and compared with the corresponding developmental curves obtained in vivo. Both acetylcholinesterase and choline acetyltransferase activities in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965466
更新日期：1977-08-01 00:00:00
abstract：:A study was undertaken in rats to evaluate the effects of short-term oral ethanol administration on the levels of fatty acid ethyl esters (FAEE) in brain and peripheral organs in the presence and absence of pretreatment with L-carnitine. Administration of ethanol to rats for seven days resulted in fatty acid ethyl est...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020984114824
更新日期：1999-01-01 00:00:00
abstract：:We investigated the ability of hydrogen peroxide (H2O2) to cause apoptotic cell death in cultured rat forebrain neurons and the potential mechanisms by which oxidative stress triggers delayed neuronal death. H2O2 (25 microM for 5 min) reduced cell viability to 34.5 +/- 8.3% of untreated controls 20 h after exposure, a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022403224901
更新日期：1997-03-01 00:00:00
abstract：:Convulsant doses of penicillin and elevated ambient pressure of 41 bar enhance the excitability of neurons. Their effects have been studied in neostriatal tissue with methods allowing differentiation between transmitter and metabolic glutamate pools. Levels of glutamate (Glu), glutamine (Gln), aspartate (Asp); gamma-a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965621
更新日期：1989-11-01 00:00:00
abstract：:Metformin is the most frequently used drug for the treatment of type 2 diabetes in humans. However, only little is known about effects of metformin on brain metabolism. To investigate potential metabolic consequences of an exposure of brain cells to metformin, we incubated rat astrocyte-rich primary cultures with this...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1733-8
更新日期：2017-01-01 00:00:00
abstract：:Neurons, purified by bulk-isolation procedures from 10 day-old rat brain, are 80-90% homogeneous. Contaminants are primarily blood vessels and occasional oligodendroglia. All sizes and shapes of neurons are obtained, as they are isolated from all areas of the brain, except the cerebellum. Neurotransmitter enzyme activ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00971368
更新日期：1987-01-01 00:00:00
