Abstract：
:Unrelated cells such as dental follicle precursor cells (DFPCs) and retinal Müller cells (MCs) make spheres after cultivation in serum-replacement medium (SRM). Until today, the relation and molecular processes of sphere formation from different cell types remain undescribed. Thus in this study we compared proteomes of spheres derived from MCs and DFPCs. 73% of 676 identified proteins were similar expressed in both cell types and many of them are expressed in the brain (55%). Moreover proteins are overrepresented that are associated with pathways for neural diseases such as Huntington disease or Alzheimer disease. Interestingly up-regulated proteins in DFPCs are involved in the biosynthesis of glycosphingolipids. These lipids are components of gangliosides such as GD3, which is a novel neural stem cell marker. In conclusion spheres from different types of cells have highly similar proteomes. These proteomes probably show essential cellular processes in neurosphere-like cell clusters.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Beck HC,Petersen J,Felthaus O,Schmalz G,Morsczeck Cdoi
10.1007/s11064-011-0524-0subject
Has Abstractpub_date
2011-11-01 00:00:00pages
2002-7issue
11eissn
0364-3190issn
1573-6903journal_volume
36pub_type
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