Abstract：
:In the present study, we evaluated the expression of some proliferation and differentiation markers in 15 DIV astrocyte cultures pretreated or not with 0.5 mM glutamate for 24 h and than maintained under chronic or acute treatment with 50 μM R(+)enantiomer or raceme alpha-lipoic acid (ALA). GFAP expression significantly increased after (R+)enantiomer acute-treatment and also in glutamate-pretreated ones. Vimentin expression increased after R(+)enantiomer acute-treatment, but it decreased after raceme acute-treatment. Nestin expression drastically increased after acute raceme-treatment in glutamate-pretreated or not cultures, but significantly decreased after (R+)enantiomer acute and chronic-treatments. Cyclin D1 expression increased in raceme acute-treated cultures pretreated with glutamate. MAP-kinase expression slightly increased after (R+)enantiomer acute treatment in glutamate-pretreated or unpretreated ones. These preliminary findings may better clarify antioxidant and metabolic role played by ALA in proliferating and differentiating astrocyte cultures suggesting an interactive cross-talk between glial and neuronal cells, after brain lesions or damages.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Bramanti V,Tomassoni D,Bronzi D,Grasso S,Currò M,Avitabile M,Li Volsi G,Renis M,Ientile R,Amenta F,Avola Rdoi
10.1007/s11064-010-0256-6subject
Has Abstractpub_date
2010-12-01 00:00:00pages
2070-7issue
12eissn
0364-3190issn
1573-6903journal_volume
35pub_type
杂志文章abstract：:Brain regional 5-HT (5-hydroxytryptamine) and 5-HIAA (5-hydroxyindole-3-acetic acid) concentrations in male LE rats at three times were measured by a fluorescence method, to evaluate effects of intracranial surgery and administration of melatonin on the changes in these compounds during the first part of the dark phas...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965929
更新日期：1989-10-01 00:00:00
abstract：:The effect of maternal hypoxia on the modification of the fetal brain cell membrane N-methyl-D-aspartate (NMDA) receptor and its modulatory sites was investigated. Experiments were conducted in pregnant guinea pigs of 60 days of gestation. Guinea pig fetuses were exposed to maternal hypoxia (FiO2 = 7%) for 60 minutes....
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968402
更新日期：1992-12-01 00:00:00
abstract：:Release of endogenous dopamine (DA) and gamma-aminobutyric acid (GABA) from superfused rat caudate synaptosomes was monitored with liquid chromatography with electrochemical detection. Dopamine was analyzed by oxidative detection following alumina extraction while GABA was analyzed with reductive detection following p...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964602
更新日期：1985-03-01 00:00:00
abstract：:Gangliosides, the acidic glycosphingolipids (GSLs) containing N-acetylgalactosamine and sialic acid are ubiquitous in the central nervous system. At least six DSL-glycosyltransferase activities (GLTs Gangliosides, the acidic glycosphingolipids (GSLs) containing N-acetylgalactosamine and sialic acid (or NAc-Neuraminic ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0762-9
更新日期：2012-06-01 00:00:00
abstract：:The influence of phosphatidylserine (PS) on the isoniazid-induced convulsions has been studied in mice. Sonicated dispersions of this phospholipid given intravenously do not show anticonvulsant activity but they do so when gamma-aminobutyric acid (GABA) is simultaneously injected. GABA alone is inactive. The synergism...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964802
更新日期：1984-08-01 00:00:00
abstract：:Calpains, cathepsins and caspases play crucial role in mediating cell death. In the present study we observed a cascade of events involving the three proteases during middle cerebral artery occlusion (MCAo) in Wistar rats. The rats were MCA occluded and reperfused at various time points. We observed a maximal increase...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9567-7
更新日期：2008-11-01 00:00:00
abstract：:The recent publication of the human genome sequence provides an opportunity both to combat diseases that are presently considered as pharmaceutically intractable and also to improve current therapies for many common human diseases. The identification of every human gene by ongoing bioinformatic efforts has the potenti...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022349805831
更新日期：2003-02-01 00:00:00
abstract：:The action of electroacupuncture (EA) may be similar to analgesia by electrode stimulation or transcutaneous nerve stimulation. Since EA may directly stimulate nerve activity or indirectly enhance the release of opiate peptides and other neurotransmitter substances, we have used (Na+ + K+)-ATPase as a model to study t...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964513
更新日期：1984-05-01 00:00:00
abstract：:The modulation of intracellular GABA activated 36Cl- in-->out permeation across single Deiters' neuron membranes has been studied in a microchamber system. Addition of Mg2+/ATP on the membrane cytoplasmic side reduces strongly the GABA effect as does ATP alone. However, the greatest inhibition of the GABA effect is gi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021073230464
更新日期：1999-08-01 00:00:00
abstract：:The antibodies against omega-conotoxin GVIA (omega-CTX GVIA; N-type voltage-dependent calcium channel [VDCC] blocker) and B1Nt (N-terminal segment [residues 1-13] of BI alpha1 subunits of VDCCs) were prepared, and the selectivity for each antigen omega-CTX GVIA and B1Nt was investigated. For the antigen selectivity of...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1023208103438
更新日期：2003-06-01 00:00:00
abstract：:A variety of naturally occurring amino acids, their isomers, and synthetic analogs were tested for their ability to inhibit uptake of [3H]glutamate into presynaptic vesicles from bovine cerebral cortex. Strongest inhibition (Ki < 1mM) was observed for trans-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) and erythr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966925
更新日期：1993-01-01 00:00:00
abstract：:Germline mutations in genes encoding succinate dehydrogenase subunits are associated with the development of familial pheochromocytomas and paragangliomas [hereditary paraganglioma/pheochromocytoma syndrome (HPPS)]. In particular, a mutation in succinate dehydrogenase subunit B (SDHB) is highly associated with abdomin...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1738-3
更新日期：2016-04-01 00:00:00
abstract：:The concentrations of dopamine (DA), 5-hydroxytryptamine (5-HT) and noradrenaline (NA) in the rat vas deferens divided in eight or four sections were determined by high performance liquid chromatography with electrochemical detection. Dopamine and NA had the same regional distribution; their concentrations were maxima...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00970770
更新日期：1988-10-01 00:00:00
abstract：:Changes in serotonin (5-HT) and 5-hydroxy indole acetic acid (5-HIAA), its major metabolite, in cerebral cortex, corpus striatum and hippocampus were investigated at 10th and 21st days of chronic ethanol ingestion in Wistar rats. Ethanol (7.2% v/v) was given to rats in a modified liquid diet. Biochemical analysis was ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007579705230
更新日期：2000-02-01 00:00:00
abstract：:The tyrosine phosphatase inhibitor BpV(phen) stimulated a concentration-dependent increase of phospholipase C (PLC) activity in bovine adrenal medullary chromaffin cells. This response was accompanied by an increase in PLCgamma1 tyrosine phosphorylation and its cytosketetal translocation. Insulin, at high concentratio...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000042211.76499.c6
更新日期：2004-10-01 00:00:00
abstract：:Protocatechuic acid (PA), a major metabolite of anthocyanins, has been reported to possess antioxidant and anti-inflammatory activities. However, the effects of PA on LPS-induced inflammatory responses in microglia have not been reported. The aim of this study was to investigate the anti-inflammatory effects and molec...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1646-6
更新日期：2015-08-01 00:00:00
abstract：:It is well known that the principal biomolecules involved in Alzheimer's disease (AD) are acetylcholinesterase (AChE), acetylcholine (ACh) and the amyloid beta peptide of 42 amino acid residues (Aβ42). ACh plays an important role in human memory and learning, but it is susceptible to hydrolysis by AChE, while the aggr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1400-5
更新日期：2014-11-01 00:00:00
abstract：:Nicotine is the principal psychoactive ingredient in cigarette smoke, and has been associated with health problems in humans. However, the pure form of nicotine may prove to be a valuable pharmaceutical agent for the treatment of AD. However, the beneficial effects of nicotine remain a matter of much controversy. In o...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9629-5
更新日期：2008-09-01 00:00:00
abstract：:The activation of caspase-3 is considered to be a reliable marker for apoptotic cell death, and a 120-kDa fragment of αII-spectrin is generated by caspase-3 mediated cleavage of this structural protein. In the present study, we compared cleaved αII-spectrin (120-kDa) and cleaved caspase-3-immunoreactive cells and thei...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0633-9
更新日期：2012-03-01 00:00:00
abstract：:N-terminal protein arginylation has been demonstrated in vitro and in situ and has been reported to increase following injury to sciatic nerves of rats. The present study attempts to demonstrate these reactions in vivo by applying [3H]Arg to the cut end of sciatic nerves in anesthetized rats and assaying for N-termina...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021998227237
更新日期：1997-12-01 00:00:00
abstract：:In the experimental setting, taurine is known to be released from swollen cells to reestablish their normal volume. However, its clinical relevance has not been fully understood. This study was undertaken to reveal changes in cerebrospinal fluid (CSF) amino acids concentration in patients with severe traumatic brain i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-9693-4
更新日期：2005-01-01 00:00:00
abstract：:The concentrations of taurine in a number of brain regions and in other tissues of rhesus monkeys fed a taurine-free human infant formula for 3 months are substantially lower than in similar monkeys fed the same formula supplemented with taurine. Activities of enzymes involved in taurine biosynthesis were not differen...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00972479
更新日期：1988-04-01 00:00:00
abstract：:Epilepsy is a disorder of the brain characterized by an enduring predisposition to generate epileptic seizures. The glycolytic inhibitor 2-deoxy-D-glucose (2-DG) has been reported to exert antiepileptic effects by upregulating KATP subunits (kir6.1 and kir6.2). We evaluated whether 2-DG exhibits anti-seizure effect by...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02734-3
更新日期：2019-04-01 00:00:00
abstract：:McGill-R-Thy1-APP rats express the human amyloid precursor protein carrying the Swedish and Indiana mutations. We examined the neurochemical content of the dorsal hippocampus in three-months-old male and female transgenic rats and healthy age- and gender-matched controls using in vivo (1)H MRS in order to assess early...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1222-x
更新日期：2014-02-01 00:00:00
abstract：:This study attempted to investigate the possible involvement of the brain stem noradrenergic system in the development of hypertension in spontaneously hypertensive rats. Steady-state norepinephrine, dopamine, serotonin and 5-hydroxyindoleacetic acid concentrations and norepinephrine turnover were determined in the in...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969761
更新日期：1989-01-01 00:00:00
abstract：:Treatment for epilepsy, particularly temporal lobe epilepsy, is challenging. Baicalein has multiple effects, including anti-inflammatory action. However, little is known about its efficacy in treatment of epilepsy. In this study, we established a pilocarpine-induced rat model and used it for assessment of baicalein ef...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03150-8
更新日期：2020-12-01 00:00:00
abstract：:Glutamine synthetase (GS) is the key enzyme in cerebral glutamine production. Understanding the regulation of the expression of GS is important for definition of the control of glutamine metabolism in brain. Therefore, we studied the control of GS expression by 8-bromo cyclic AMP in primary cultures of astrocytes prep...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973746
更新日期：1990-06-01 00:00:00
abstract：:Effects of single and repeated doses of acrylamide on fast and slow axonal transport of radio labeled proteins following the injection of L-[4,5-3H] leucine have been studied in the optic system of male Sprague-Dawley rats. A single dose of acrylamide (100 mg/kg) had no effect, but higher concentrations (200-300 mg/kg...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973750
更新日期：1990-06-01 00:00:00
abstract：:Myelinating cells of both the peripheral and central nervous systems (CNSs) undergo dramatic cytoskeletal reorganization in order to differentiate and produce myelin. Myelinating oligodendrocytes in the CNS show a periodic actin pattern, demonstrating tight regulation of actin. Furthermore, recent data demonstrate tha...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-019-02753-0
更新日期：2020-03-01 00:00:00
abstract：:Acute tryptophan depletion (ATD) is extensively used to investigate the role of central serotonin (5-HT). However, several studies reported that ATD had no significant effect on central 5-HT concentration and some ATD-induced changes was independent of 5-HT in the rodent brain. Therefore, the potential mechanism of AT...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1177-y
更新日期：2013-12-01 00:00:00
