Abstract：
:Release of endogenous dopamine (DA) and gamma-aminobutyric acid (GABA) from superfused rat caudate synaptosomes was monitored with liquid chromatography with electrochemical detection. Dopamine was analyzed by oxidative detection following alumina extraction while GABA was analyzed with reductive detection following pre-column derivatization with trinitrobenzenesulfonic acid and extraction. Both spontaneous and K+-stimulated (40 mM) release were examined as well as the effect of several possible neuromodulatory agents (DA, GABA, muscimol, ascorbic acid, acetylcholine). The content of GABA in the sample and the amount released by K+ were approximately fifty times those of DA although the relative amounts released by repetitive K+ stimulations were similar. Muscimol and DA significantly attenuated both the spontaneous and stimulated release of GABA while ascorbate and acetylcholine had no effect. Acetylcholine significantly increased both the stimulated and spontaneous release of DA while the other agents had no effect. Dopamine showed an absolute dependence on calcium for stimulated release while GABA exhibited a significant calcium-independent release. These results indicate that profound differences exist in the factors which modulate the release of endogenous DA and GABA.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Caudill WL,Bigelow JC,Wightman RMdoi
10.1007/BF00964602subject
Has Abstractpub_date
1985-03-01 00:00:00pages
319-31issue
3eissn
0364-3190issn
1573-6903journal_volume
10pub_type
杂志文章abstract：:Recent evidence indicates that mechanisms involved in reward and mechanisms involved in learning interact, in that reward includes learning processes and learning includes reward processes. In spite of such interactions, reward and learning represent distinct functions. In the present study, as part of an examination ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9343-8
更新日期：2007-10-01 00:00:00
abstract：:Axonal regeneration across the site of spinal cord lesion is often aborted in adult mammalian species. The use of DNA vaccine to nullify the inhibitory molecules has been shown to be effective in promoting axonal regeneration in injured spinal cord. The possible molecular mechanisms, however, remain to be elucidated. ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-9951-6
更新日期：2009-09-01 00:00:00
abstract：:We have synthesized the tertiary amines of pyridostigmine and neostigmine, 3-pyridinol dimethylcarbamate (norpyridostigmine) and 3-dimethylaminophenol dimethylcarbamate (norneostigmine) respectively, and we have tested their abilities to cross the blood-brain barrier and inhibit mouse brain AChE activity. The in vivo ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973747
更新日期：1990-06-01 00:00:00
abstract：:2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) was phosphorylated in vivo, in brain slices and in a cell free system. Phosphoamino acid analysis of immunoprecipitated CNP labeled in vivo and in brain slices revealed phosphorylation of phosphoserine (94%) and phosphothreonine (5%) residues. Phosphorylation of CNP in...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00967712
更新日期：1994-06-01 00:00:00
abstract：:Human dental follicle cells (DFCs) are ectomesenchymal multipotent stem cells that form spheroid cell clusters (SCCs) under serum free medium cell culture conditions (SFM). Until today, molecular mechanisms for the formation of SCCs are unknown. In this study a quantitative phosphoproteomics approach revealed regulate...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1298-y
更新日期：2014-07-01 00:00:00
abstract：:There is increasing evidence to suggest that hepatic encephalopathy in acute liver failure is the result of altered glutamatergic function. In particular, the high affinity uptake of glutamate is decreased in brain slices and synaptosomes from rats with acute liver failure as well as by exposure of cultured astrocytes...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022532623281
更新日期：1999-11-01 00:00:00
abstract：:It is thought that changes in gene expression in the brain mediate chronic ethanol-induced complex behaviors such as tolerance, dependence, and sensitization, and also relate to ethanol-induced brain toxicity. Using high-density filter-based cDNA microarrays (GeneFilters), we analyzed the expression of over 5000 genes...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020937728506
更新日期：2002-10-01 00:00:00
abstract：:A set of synaptic membrane antigens has been investigated in a number of tissues by indirect immunofluorescence histochemistry, using an antiserum (SPM-I) raised against a purified synaptosomal plasma membrane fraction prepared from day-old chick forebrain. The antigens were found to be present in both the central and...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966022
更新日期：1977-02-01 00:00:00
abstract：:Omega-conotoxin GVIA (omega-CTX), as a selective blocker for an N-type Ca2+ channel, has been conveniently used in many molecular biochemical and pharmacological experiments. There has been little elucidation of 125I-omega-CTX binding sites (mainly the 135-kDa band) in the crude membranes from chick brain, although th...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022805615926
更新日期：2003-05-01 00:00:00
abstract：:This study was undertaken to evaluate whether chemical hypoxia-induced cell injury is a result of reactive oxygen species (ROS) generation, ATP depletion, mitochondrial permeability transition, and an increase in intracellular Ca2+, in A172 cells, a human glioma cell line. Chemical hypoxia was induced by incubating ce...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1024280429036
更新日期：2003-08-01 00:00:00
abstract：:N-terminal protein arginylation has been demonstrated in vitro and in situ and has been reported to increase following injury to sciatic nerves of rats. The present study attempts to demonstrate these reactions in vivo by applying [3H]Arg to the cut end of sciatic nerves in anesthetized rats and assaying for N-termina...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021998227237
更新日期：1997-12-01 00:00:00
abstract：:Tyrosinemia type II, also known as Richner-Hanhart syndrome, is an autosomal recessive inborn error of metabolism caused by a deficiency of hepatic cytosolic tyrosine aminotransferase, and is associated with neurologic and development difficulties in numerous patients. Considering that the mechanisms underlying the ne...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1180-3
更新日期：2013-12-01 00:00:00
abstract：:It has been reported that electrical stimulation (ES) can promote nerve regeneration. One of the key factors leading to the promotion is calcium, which plays a vital role in the regulation on electrical activity of neurons, but the detailed mechanism is still an open question. In this study, ES was used to stimulate c...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1197-7
更新日期：2014-01-01 00:00:00
abstract：:The influence of hypothyroidism (HO) induced by treatment with propylthiouracil on lipid composition, receptor responsiveness of M1-muscarinic receptors (M1AChRs) and metabotropic glutamate receptors (mGluRs) as well as on protein kinase C (PKC) activity was investigated in the cerebral cortex of Lewis rats. HO did no...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02527750
更新日期：1996-05-01 00:00:00
abstract：:Metformin is the most frequently used drug for the treatment of type 2 diabetes in humans. However, only little is known about effects of metformin on brain metabolism. To investigate potential metabolic consequences of an exposure of brain cells to metformin, we incubated rat astrocyte-rich primary cultures with this...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1733-8
更新日期：2017-01-01 00:00:00
abstract：:Protein misfolding and deposition in the brain are implicated in the etiology of numerous neurodegenerative disorders. Here, organic solutes characteristic of microorganisms adapted to hot environments, were tested on experimental cell models of Huntington's and Parkinson's diseases. Diglycerol phosphate, di-myo-inosi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0440-3
更新日期：2011-06-01 00:00:00
abstract：:Experimentalists would like to perform large-scale expression analysis on samples drawn from small cell populations and even single cells. Amplification of the source material is required to assay such small samples under all existing array technologies. In this report we consider the very different theoretical proper...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020917223962
更新日期：2002-10-01 00:00:00
abstract：:Propofol and ketamine may provide certain degree of neuroprotection, but the underlying mechanism remains unclear to date. The cAMP response element-binding protein (CREB) was proposed that its phosphorylation at Ser133 (P-CREB) constituted a convergence point involved in neuroprotection. The purpose of this study was...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0582-3
更新日期：2012-01-01 00:00:00
abstract：:Because it appears that oxidative stress and inflammation are implicated with disease pathogenesis in the diabetic brain, many researchers have used streptozotocin (STZ)-induced diabetic animals to study superoxide production and the effects of superoxide scavengers like Cu,Zn-superoxide dismutase (SOD1). However, man...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0280-6
更新日期：2011-01-01 00:00:00
abstract：:The Editors have retracted this article [1] following an investigation conducted by the journal. After publication concerns were raised regarding interpretation of the data presented in Fig. 4. The Editors requested additional data and clarification to confirm interpretation of data results. After further review, the ...
journal_title：Neurochemical research
pub_type： 杂志文章,撤回出版物
doi：10.1007/s11064-020-03077-0
更新日期：2020-09-01 00:00:00
abstract：:Pathophysiological concentrations of branched chain keto-acids (BCKAs), such as those that occur in maple syrup urine disease, inhibit oxygen consumption in liver homogenates and brain slices and the enzymatic activity of alpha-ketoglutarate- and pyruvate dehydrogenase complexes. Consistent with previous work, studies...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9291-3
更新日期：2007-04-01 00:00:00
abstract：:Methylguanidine (MG) is known as not only a nephrotoxin but also as a neurotoxine. We have previously showed that MG itself generates hydroxyl radicals (*OH) in an in vitro study. In this study, we examined the inhibitory effects of ascorbate, EPC-K(1) (alpha-tocopheryl-L-ascorbate-2-O-phosphate diester), Trolox (wate...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9811-9
更新日期：2009-04-01 00:00:00
abstract：:The amyloid peptide (Abeta) deposited in Alzheimer's disease (AD) is generated by beta- and gamma-secretase processing of a larger integral membrane protein precursor (APP). Intramembrane processing of APP by gamma-secretase also yields an intracellular fragment, CTFgamma (a.k.a. AICD), which is highly conserved and i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9449-z
更新日期：2007-12-01 00:00:00
abstract：:The SAM strains, a group of related inbred strains consisting of senescence-prone inbred strains (SAMP) and senescence-resistant inbred strains (SAMR), have been successfully developed by selective inbreeding of the AKR/J strain of mice donated by the Jackson laboratory in 1968. The characteristic feature of aging com...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-009-9922-y
更新日期：2009-04-01 00:00:00
abstract：:Parkinson's disease (PD) is a neurodegenerative disorder of dopaminergic, noradrenergic, and serotonergic systems, in which dopamine, noradrenaline, and serotonin levels are depleted and lead to the development of motor and non-motor symptoms such as tremor, bradykinesia, weight changes, fatigue, depression, and visua...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03130-y
更新日期：2020-11-01 00:00:00
abstract：:The maintaining of homeostasis in the organism in response to a variable environment is provided by the highly hierarchic neuroendocrine-immune system. The crucial component of this system is the hypothalamus providing the endocrine regulation of key peripheral organs, and the adenohypophysis. In this case, neuron-der...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0127-1
更新日期：2010-06-01 00:00:00
abstract：:Using the mouse maximal electroshock test, the reference model of tonic-clonic seizures, the aim of the present study was to determine the type of interaction between mexiletine (a class IB antiarrhythmic drug) and classical antiepileptics: valproate, carbamazepine, phenytoin, and phenobarbital. Isobolographic analysi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1812-x
更新日期：2016-05-01 00:00:00
abstract：:In our previous study vesamicol, an inhibitor of the acetylcholine transporter of the cholinergic vesicles, inhibited veratridine-evoked external Ca(2+)-dependent acetylcholine release from striatal slices but did not influence acetylcholine release observed in Ca(2+)-free medium (4). Here we examined if the effect of...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968780
更新日期：1992-06-01 00:00:00
abstract：:The presence and specificity of insulin receptors was investigated in culture cells obtained from 15-16 days old embryonic mouse cerebra. Developmental studies suggested that the maximum insulin binding occurred at about 11 days in vitro (DIV). Scatchard analysis of binding data revealed two types of binding sites. On...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01268877
更新日期：1988-05-01 00:00:00
abstract：:Emerging data suggests that mitochondrial dysfunction is prominently involved in Alzheimer disease (AD) progression. Sirtuin-3 (Sirt3) is a member of the sirtuin family of nicotinamide adenine dinucleotide dependent deacetylases that regulates a variety of mitochondrial functions and suppresses mitochondria-related ph...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1630-1
更新日期：2015-08-01 00:00:00
