Abstract：
:The maximal rate of some cerebral enzymatic activities related to energy transduction (hexokinase; phosphofructokinase; lactate dehydrogenase; citrate synthase; malate dehydrogenase; total NADH-cytochrome c reductase; cytochrome oxidase), amino acid metabolism (glutamate decarboxylase; glutamate dehydrogenase) and cholinergic metabolism (acetylcholine esterase) were tested in the cerebral cortex and in sub-cortical area of rats. The evaluations were performed both in the homogenate in toto and in the crude mitochondrial fraction, before and after a postdecapitative normothermic ischemia of 5, 10, 20, and 40 min duration. The results are discussed also with respect to the pharmacological pretreatment with two biological substances which may modulate amino acid (L-alanine) and phospholipid metabolism (CDP-choline). The analysis of the present data suggests the occurrence in brain tissue of a variety of interrelated factors implicated in the ischemia-induced changes of the maximal rate of the enzymatic activities related to the energy transduction. These include: (a) rearrangement of the enzymatic activities because of the changed metabolic and chemico-physical condition; (b) decrease in the activity of enzymes related to the electron transfer chain and glycolysis; (c) changes in enzymes related to mitochondrial membranes. The effects of in vivo administration of alanine or CDP-choline, even if significant, are not consistent throughout the time period studied.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Villa RF,Marzatico F,Benzi Gdoi
10.1007/BF00963926subject
Has Abstractpub_date
1983-02-01 00:00:00pages
269-90issue
2eissn
0364-3190issn
1573-6903journal_volume
8pub_type
杂志文章abstract：:Epilepsy is a disorder of the brain characterized by an enduring predisposition to generate epileptic seizures. The glycolytic inhibitor 2-deoxy-D-glucose (2-DG) has been reported to exert antiepileptic effects by upregulating KATP subunits (kir6.1 and kir6.2). We evaluated whether 2-DG exhibits anti-seizure effect by...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02734-3
更新日期：2019-04-01 00:00:00
abstract：:The copper-transporting ATPases Atp7A and Atp7B play a major role in controlling intracellular copper levels. In addition, they are believed to deliver copper to the copper-requiring proteins destined for the secretory vesicles. One cuproprotein, dopamine beta-hydroxylase (DBH) functions in the biosynthesis of norepin...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1023219308890
更新日期：2003-06-01 00:00:00
abstract：:Analysis in mouse brain slices of the uptake of acetyl-L-[N-methyl-14C]carnitine with time showed it to be concentrative, and kinetic analysis gave a Km of 1.92 mM and a Vmax of 1.96 mumol/min per ml, indicating the presence of a low-affinity carrier system. The uptake was energy-requiring and sodium-dependent, being ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964865
更新日期：1989-05-01 00:00:00
abstract：:This review presents data on metabotropic uridine nucleotide receptor subtypes (P2UR) activated by UTP, sometimes also by UDP and/or ATP. Some chemical details of receptor subtypes and ligand interactions are described. Ligand-activated P2UR subtypes may couple to different second messengers, yet little is known about...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022487128766
更新日期：1997-08-01 00:00:00
abstract：:The labeling of RNA in young and adult rat brain has been studied by measuring in vitro (tissue slices incubation) the incorporation of labeled uridine into RNA of total tissue and of the various subcellular fractions purified from cerebral hemispheres of 1- and 10-month-old rats. Gel electrophoretic analysis of the n...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965100
更新日期：1983-04-01 00:00:00
abstract：:Ion channels are targets of various antiepileptic drugs. In cerebral presynaptic nerve endings Na(+) and Ca(2+) channels are particularly abundant, as they control neurotransmitter release, including the release of glutamate (Glu), the most concentrated excitatory amino acid neurotransmitter in the brain. Several pre-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1749-0
更新日期：2016-04-01 00:00:00
abstract：:Eclalbasaponin II derived from Eclipta prostrata L. (Asteraceae) has been reported to have anti-fibrotic, anti-bacterial and autophagic activities, but its effect on cognitive function has not been investigated. We studied the effect of eclalbasaponin II on cholinergic blockade-induced memory impairment in mice using ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2430-6
更新日期：2018-02-01 00:00:00
abstract：:Amyotrophic lateral sclerosis (ALS) is an adult-onset, irreversible neurodegenerative disease that leads to progressive paralysis and inevitable death 3-5 years after diagnosis. The mechanisms underlying this process remain unknown, but new evidence indicates that accumulating levels of D-serine result from the downre...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-2131-6
更新日期：2017-04-01 00:00:00
abstract：:The effects of mu, delta, and kappa receptor-agonists on forskolin stimulated cyclic adenosine-3',5'-monophosphate (cAMP) formation were examined in astroglial enriched primary cultures from the cerebral cortex of newborn rats. Intracellular cAMP accumulation was quantified by radioimmunoassay. Morphine was used as a ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01101714
更新日期：1990-11-01 00:00:00
abstract：:In this paper we propose a powerful procedure to measure functional activation of the mouse delta-opioid receptor transiently expressed in mammalian cells. Receptor stimulation was assessed using a population of electroporated COS cells, transfected at a 50% efficiency. Under those conditions, agonist-promoted activat...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02532371
更新日期：1996-11-01 00:00:00
abstract：:1-Methyl-4-phenylpyridinium (MPP(+)) or 6-hydroxydopamine (6-OHDA) caused a nuclear damage, the mitochondrial membrane permeability changes, leading to the cytochrome c release and caspase-3 activation, the formation of reactive oxygen species and the depletion of GSH in PC12 cells. Nicardipine (a calcium channel bloc...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9088-9
更新日期：2006-07-01 00:00:00
abstract：:Treatment for epilepsy, particularly temporal lobe epilepsy, is challenging. Baicalein has multiple effects, including anti-inflammatory action. However, little is known about its efficacy in treatment of epilepsy. In this study, we established a pilocarpine-induced rat model and used it for assessment of baicalein ef...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03150-8
更新日期：2020-12-01 00:00:00
abstract：:It is reported that CNS hemorrhage causes membrane dysfunction and may exacerbate this damage as a result of secondary ischemia or hypoxia. Since hyperbaric oxygenation improves oxygen metabolism, it may reduce this membrane damage. The present study was conducted to reveal whether hyperbaric oxygenation influences me...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966765
更新日期：1993-09-01 00:00:00
abstract：:Incubation of [3H]-tyrosine methionine5-enkephalin (MET) with human brain preparations (100,000g supernatant; sections of the limbic system, thalamus, basal ganglia, cerebellum, and cortex) results in its rapid and complete degradation; over 95% of the initial labeled tyrosine is recovered as the free aminoacid within...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9418-6
更新日期：2008-01-01 00:00:00
abstract：:These studies were designed to compare the effects of nitric oxide (NO) generating compounds with those of several iron containing compounds which do not generate NO on glutamate receptor function. Stimulation of primary cultures of cerebellar granule cells with N-methyl-D-aspartate (NMDA) or kainate results in the el...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969689
更新日期：1995-07-01 00:00:00
abstract：:The release of endogenous amino acids from depolarized rat hippocampal mossy fiber synaptosomes was investigated to assess the possible role(s) of glutamate and aspartate in mediating the excitatory mossy fiber synaptic input. The relative proportions of prodynorphin-derived peptides concomitantly released with amino ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969176
更新日期：1990-01-01 00:00:00
abstract：:The hippocampus-derived neuroestradiol plays a major role in neuroplasticity, independent of circulating estradiol that originates from gonads. The response of hypothalamus-pituitary regions towards the synthesis of neuroestradiol in the hippocampus is an emerging scientific concept in cognitive neuroscience. Hippocam...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-019-02833-1
更新日期：2019-08-01 00:00:00
abstract：:Ciliary neurotrophic factor (CNTF) is a promyelinating trophic factor that plays an important role in multiple sclerosis (MS). However, mechanisms by which CNTF expression could be increased in the brain are poorly understood. Recently we have discovered anti-inflammatory and immunomodulatory activities of sodium benz...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1723-x
更新日期：2015-11-01 00:00:00
abstract：:The concentrations of taurine in a number of brain regions and in other tissues of rhesus monkeys fed a taurine-free human infant formula for 3 months are substantially lower than in similar monkeys fed the same formula supplemented with taurine. Activities of enzymes involved in taurine biosynthesis were not differen...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00972479
更新日期：1988-04-01 00:00:00
abstract：:The effect of isoproterenol, norepinephrine, dopamine, and serotonin on the accumulation of adenosine 3',5'-monophosphate (cAMP) was studied in homogeneous neuronal cultures from 8-day chick embryo hemispheres. Among the catecholamines, isoproterenol had a more pronounced effect on the accumulation of cAMP. Norepineph...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966167
更新日期：1980-10-01 00:00:00
abstract：:Exposure of rat pheochromocytoma PC12 cells to 0.1 mM 6-aminonicotinamide (6AN) for 24 hours resulted in a 500-fold increase in 6-phosphogluconate indicating active metabolism of glucose via the oxidative enzymes of the pentose phosphate pathway. Amounts of 6-phosphogluconate that accumulated in 6AN-treated cells at 2...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01000236
更新日期：1987-06-01 00:00:00
abstract：:Several epidemiological studies have shown that consumption of large quantities of vegetables especially cruciferous vegetables (Broccoli and Brussels sprouts) can protect against chronic diseases. Sulforaphane, an isothiocynate found in cruciferous vegetables has been demonstrated to have neuroprotective effects in s...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1965-2
更新日期：2016-10-01 00:00:00
abstract：:Systemic inflammation is present in obesity and emerging evidence, primarily from studies using male rodents fed high-fat diets, suggests neuroimmune signaling also is involved. We investigated early changes in neuroimmune signaling during the weight gain that follows ovariectomy in rats. Ovariectomized (OVX) rats wer...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2596-6
更新日期：2018-09-01 00:00:00
abstract：:Seizures are accompanied by an exacerbated activation of cerebral ion channels. 4-aminopyridine (4-AP) is a pro-convulsive agent which mechanism of action involves activation of Na(+) and Ca(2+) channels, and several antiepileptic drugs control seizures by reducing these channels permeability. The antidepressant, sert...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1840-1
更新日期：2016-06-01 00:00:00
abstract：:α-Synuclein (α-Syn) plays a crucial role in the pathophysiology of Parkinson's disease (PD), the degeneration of dopaminergic neurons. Previous studies have shown that α-Syn regulates dopamine synthesis by binding to and inhibiting tyrosine hydroxylase (TH). In neurons, protein phosphatases (PPs) play a prominent role...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1477-x
更新日期：2015-03-01 00:00:00
abstract：:The p53 tumor suppressor gene is a sequence-specific transcription factor that activates the expression of genes engaged in promoting growth arrest or cell death in response to multiple forms of cellular stress. p53 expression is elevated in damaged neurons in acute models of injury such as ischemia and epilepsy and i...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1021687810103
更新日期：2003-01-01 00:00:00
abstract：:Numerous studies have demonstrated that the Acetyl Coenzyme A-dependent arylamine NAT enzyme exist in many tissues of experimental animals including humans, and that NAT has been shown to be exist in mouse brain tissue. Increased NAT activity levels are associated with increased sensitivity to the mutagenic effects of...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007632326953
更新日期：2000-11-01 00:00:00
abstract：:Glioblastoma (GBM) is the most aggressive and lethal form of brain tumor. However, therapeutic strategies against malignant gliomas have not been completely established. Runt-related transcription factor 2 (Runx2) is an essential gene for skeletal development but its regulatory role in the malignant progression of gli...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2626-4
更新日期：2018-11-01 00:00:00
abstract：:Metabolic modeling of dynamic (13)C labeling curves during infusion of (13)C-labeled substrates allows quantitative measurements of metabolic rates in vivo. However metabolic modeling studies performed in the brain to date have only modeled time courses of total isotopic enrichment at individual carbon positions (posi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0782-5
更新日期：2012-11-01 00:00:00
abstract：:This study examined the relationship between the distance of axotomy and the death of injured retinal ganglion cells (RGCs) in adult hamsters and the relationship of cytochrome c and caspase-3 on the death pathway of RGCs. The left optic nerve (ON) of adult hamsters was transected either at 1 or 3 mm away from the opt...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-6889-6
更新日期：2004-11-01 00:00:00
