Abstract：
:We studied the dose-response characteristics and the temporal profile of inhibition of brain nitric oxide (NO) synthase (NOS) elicited by i.v. administration of the NOS inhibitor nitro-L-arginine methyl ester (L-NAME). L-NAME was administered i.v. in awake rats equipped with a venous cannula. L-NAME was injected in cumulative doses of 5, 10, 20 and 40 mg/kg and rats were sacrificed 30 min after the last dose. NOS catalytic activity was assayed in forebrain cytosol as the conversion of [3H]L-arginine into [3H]L-citrulline. L-NAME attenuated brain NOS activity in a dose-dependent manner but enzyme activity could not be inhibited by more than approximately 50%. After a single 20 mg/kg injection of L-NAME the inhibition of brain NOS activity was time dependent and reached a stable level at 2 hrs (52% of vehicle). Inhibition after a single injection was still present at 96 hrs, albeit to a lower magnitude. We conclude that intravenous administration of L-NAME in rats at concentrations commonly used in physiological experiments leads to a dose and time-dependent but partial inhibition of brain NOS catalytic activity. The finding that the inhibition persists for several days after a single administration is consistent with the hypothesis that nitro-L-arginine, the active principle of L-NAME, binds to NOS irreversibly.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Iadecola C,Xu X,Zhang F,Hu J,el-Fakahany EEdoi
10.1007/BF00967330subject
Has Abstractpub_date
1994-04-01 00:00:00pages
501-5issue
4eissn
0364-3190issn
1573-6903journal_volume
19pub_type
杂志文章abstract：:The efflux kinetics of 45Ca from rat brain cortex thin slices previously equilibrated with it, was studied in a superfusion system. Two first order kinetic components of efflux from the tissue were found: k2 = 0.0667 min-1, that was unchanged by lowering the temperature from 37 degrees C to 15 degrees, and k3 = 0.0167...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966071
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abstract：:There is a growing body of evidence confirming the involvement of oxidative stress and inflammation in pathogenesis of schizophrenia. Inter-individual variation in antioxidant capacity caused by different genetic profile could potentially influence patient's susceptibility to oxidative damage. In this study we evaluat...
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pub_type： 杂志文章,随机对照试验
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abstract：:Recent studies provide solid evidence for the importance to delineate the co-relationship between preconditioning stimuli and therapeutic efficacy of drugs commonly used in clinic. However, very little is known about this important topic. In the present study, we investigated the effects of hyperthermia on the protect...
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pub_type： 杂志文章
doi：10.1007/s11064-010-0327-8
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abstract：:The kinetics and specificity of taurine and beta-alanine uptake were studied in primary cultures of rat astrocytes under identical experimental conditions. The uptake consisted of nonsaturable penetration and saturable high-affinity transport that was strictly sodium dependent. The cells accumulated taurine more effec...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00967969
更新日期：1986-02-01 00:00:00
abstract：:We have previously reported that angiotensin receptor blockade reduces reperfusion hemorrhage in a suture occlusion model of stroke, despite increasing matrix metalloproteinase (MMP-9) activity. We hypothesized that candesartan will also decrease hemorrhage associated with delayed (6 h) tissue plasminogen activator (t...
journal_title：Neurochemical research
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000023600.25994.11
更新日期：2004-06-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
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journal_title：Neurochemical research
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更新日期：1989-11-01 00:00:00
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abstract：:Diminished thiamine (vitamin B1) dependent processes and oxidative stress accompany Alzheimer's disease (AD). Thiamine deficiency in animals leads to oxidative stress. These observations suggest that thiamin may act as an antioxidant. The current experiments first tested directly whether thiamin could act as an antiox...
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更新日期：2010-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2007-03-01 00:00:00
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更新日期：2006-06-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
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journal_title：Neurochemical research
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journal_title：Neurochemical research
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更新日期：1994-07-01 00:00:00
abstract：:Kv1.3 is a voltage gated potassium channel that has been implicated in pathophysiology of multiple sclerosis (MS). In the present study we investigated temporal and cellular expression pattern of this channel in the lumbar part of spinal cords of animals with experimental autoimmune encephalomyelitis (EAE), animal mod...
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更新日期：2018-05-01 00:00:00
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更新日期：2019-05-01 00:00:00
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journal_title：Neurochemical research
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更新日期：1988-12-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：1995-01-01 00:00:00
abstract：:Methylphenidate is commonly used for the treatment of attention deficit/hyperactivity disorder. There are still few works regarding the effects of methylphenidate on brain energy metabolism. Thus, in the present study we evaluated the effect of chronic administration of methylphenidate on the activities of mitochondri...
journal_title：Neurochemical research
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更新日期：2010-11-01 00:00:00
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更新日期：2008-01-01 00:00:00
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journal_title：Neurochemical research
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更新日期：2012-02-01 00:00:00
abstract：:Microglia, the resident immune cells of the CNS, are primary regulators of the neuroimmune response to injury. Type I interferons (IFNs), including the IFNαs and IFNβ, are key cytokines in the innate immune system. Their activity is implicated in the regulation of microglial function both during development and in res...
journal_title：Neurochemical research
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更新日期：2017-09-01 00:00:00
