Abstract：
:The efflux kinetics of 45Ca from rat brain cortex thin slices previously equilibrated with it, was studied in a superfusion system. Two first order kinetic components of efflux from the tissue were found: k2 = 0.0667 min-1, that was unchanged by lowering the temperature from 37 degrees C to 15 degrees, and k3 = 0.0167 min-1 at 37 degrees C, that was reduced to 0.0897 min-1 at 15 degrees C. This suggests that k2 represents efflux from the extracellular space, and k3 that from the cellular compartment. Addition of the mitochondrial uncoupler carbonyl cyanide, m-chlorophenylhydrazone (CCCP) (10(-5)M) increased the efflux fractional rate constant of 45Ca by 35%, while no change in efflux was induced by 10 mM caffeine. Veratridine (10(-5)M) drastically reduced 45Ca efflux if superfusion was with physiological salt solution (150 mM sodium present), but not if 50 mM lithium replaced an equivalent amount of sodium in the superfusion fluid. This lithium-containing solution did not affect 45Ca efflux in the absence of veratridine. These results indicate that mitochondria accumulate only a minor fraction of intracellular 45Ca; that 45Ca possibly turns over very rapidly in the endoplasmic reticulum, and that most of 45Ca is present in a different, non-mitochondrial, non endoplasmic reticular compartment, the nature of which can be only conjectured.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Rubiales de Barioglio S,Orrego Fdoi
10.1007/BF00966071subject
Has Abstractpub_date
1982-11-01 00:00:00pages
1427-35issue
11eissn
0364-3190issn
1573-6903journal_volume
7pub_type
杂志文章abstract：:Anesthetics such as propofol can provide neuroprotective effects against cerebral ischemia. However, the underlying mechanism of this beneficial effect is not clear. Therefore, we subjected male Sprague-Dawley rats to 2 h of middle cerebral artery occlusion and investigated how post-ischemic administration of propofol...
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更新日期：2011-12-01 00:00:00
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