Abstract：
:An efficient exchange of lactate between different cell types (such as astrocytes and neurones) would require that lactate transporters are expressed in contiguous parts of the respective plasma membranes. To settle this issue we explored the subcellular expression pattern of monocarboxylate transporters (MCTs) by use of selective antibodies and high resolution immunogold cytochemistry. We investigated whether the membrane domains containing MCT1, MCT2 and MCT4 are spatially related to each other and to other membrane domains, i.e. those containing glutamate receptors. We used retina and cerebellum as a model for our investigations. We found that MCT1 was localized in the apical membrane of pigment epithelial cells and in the photoreceptor inner segment membrane in the retina. In the brain MCT1 was present in endothelial cells. MCT2 was localized in the postsynaptic membrane of parallel fiber-Purkinje cell synapses and MCT4 was situated in the membrane of glial cells in the cerebellum.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Bergersen L,Rafiki A,Ottersen OPdoi
10.1023/a:1014806723147keywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
89-96issue
1-2eissn
0364-3190issn
1573-6903journal_volume
27pub_type
杂志文章,评审abstract：:During pregnancy, emergence of endogenous opioid inhibition of oxytocin neurons is revealed by increased oxytocin secretion after administration of the opioid receptor antagonist, naloxone. Here we show that prolonged estradiol-17beta and progesterone treatment (mimicking pregnancy levels) potentiates naloxone-induced...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9506-7
更新日期：2008-04-01 00:00:00
abstract：:This study examined the relationship between the distance of axotomy and the death of injured retinal ganglion cells (RGCs) in adult hamsters and the relationship of cytochrome c and caspase-3 on the death pathway of RGCs. The left optic nerve (ON) of adult hamsters was transected either at 1 or 3 mm away from the opt...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-6889-6
更新日期：2004-11-01 00:00:00
abstract：:6-Hydroxydopamine (6-OHDA) induces the production of reactive oxygen species (ROS) that are associated with various neurodegenerative diseases such as Parkinson's disease. 3,3',4',7-Tetrahydroxyflavone (fisetin), a plant flavonoid has a variety of physiological effects such as antioxidant activity. In this study, we i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2445-z
更新日期：2018-02-01 00:00:00
abstract：:Thiamine deficiency (TD) produces severe neurodegenerative lesions. Studies have suggested that primary neurodegenerative events are associated with both oxidative stress and inflammation. Very little is known about the downstream effects on intracellular signaling pathways involved in neuronal death. The primary aim ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-02975-7
更新日期：2020-04-01 00:00:00
abstract：:Altered neurogenesis has been reported in Alzheimer disease (AD), the most common neurodegenerative disorder characterized with hyperphosphorylated tau and accumulation of β-amyloid (Aβ). Recent studies suggest that tau phosphorylation is essential for hippocampal neurogenesis, however, it is not known whether tau pho...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0316-y
更新日期：2011-02-01 00:00:00
abstract：:Disruptive effects of calcium upon neurofilaments and glial filaments were studied in white matter of rat optic nerve and spinal cord and in rat peripheral nerve. Filament ultrastructure and tissue protein composition were compared following a calcium influx into excised tissues. A calcium influx was induced by freeze...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964040
更新日期：1981-03-01 00:00:00
abstract：:Amphiphile dependency, solubility in aqueous solutions, and sensitivity to proteolysis of acetylcholinesterase (AChE) and nonspecific cholinesterase (nsChE) in the rat superior cervical ganglion were studied and compared to properties of soluble plasma cholinesterases. Ganglion AChE shows strong amphiphile dependency:...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964537
更新日期：1985-06-01 00:00:00
abstract：:The effects of chronic pentobarbital (PB) treatment on the binding characteristics of [3H]flunitrazepam (FLU) in rat brain were examined. Saline or sodium PB (500 micrograms/10 microliters/hr) was infused into the lateral cerebral ventricles of rats for 6 days using osmotic pumps. Immediately before withdrawal, there ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966726
更新日期：1994-01-01 00:00:00
abstract：:Malignant peripheral nerve sheath (MPNST) cell lines derived from patients with neurofibromatosis type 1 (NF!) were found to have basal cAMP levels which are two-fold higher than cAMP levels in normal human adult Schwann cells (nHSC). PCR analysis also revealed that normal adult human Schwann cells express mRNA for ty...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0433-2
更新日期：2011-09-01 00:00:00
abstract：:Neurons of the rat cerebral hemispheres are known to undergo a postnatal shift to a short DNA repeat length. In the present study we report that rat neuronal nuclei are more sensitive to digestion with DNAse I when isolated at a developmental stage after the shift in neuronal DNA repeated length compared to nuclei iso...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965136
更新日期：1982-08-01 00:00:00
abstract：:5- and 6-Hydroxydopamine, which we had earlier identified as naturally occurring amines in human urine, were quantified in Parkinson's patients treated with L-DOPA, Parkinson's patients whose treatment did not include L-DOPA and in age matched controls. Analysis was carried out by GC-MS of the ditrifluoromethylbenzoyl...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00978370
更新日期：1993-11-01 00:00:00
abstract：:Pyroptosis is a newly identified lytic form of programmed cell death which is characterized by plasma membrane blebbing and rupture. Pyroptosis occurs in cerebral ischemia injury, and contributes to the activation and secretion of the inflammatory cytokines interleukin (IL)-1β, IL-18, and IL-6. Previous reports have f...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02935-w
更新日期：2020-02-01 00:00:00
abstract：:The effect of optic nerve transsection on proteolytic degradation of axonally transported proteins in the superior colliculus of the rabbit was studied. Proteolysis of labeled proteins was determined in vitro in small pieces of the superior colliculus. Within 2 hours after sectioning the optic nerve there was a decrea...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973280
更新日期：1988-07-01 00:00:00
abstract：:Somatostatin (SS) is an inhibitory regulator of secretory and proliferative responses that activates a group of receptors in the plasma membrane termed SSR1-5. SSR2 is one of the most abundant SSR, which also is expressed in high numbers in many neuroendocrine tumor types. Here, we describe a study of the presence and...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022848718109
更新日期：2003-04-01 00:00:00
abstract：:Specific [3H]MK801 binding to rat brain NMDA receptors after the administration of the convulsant drug 3-mercaptopropionic acid (MP) and the adenosine analogue cyclopentyladenosine (CPA) was studied by means of a quantitative autoradiographic method. MP administration (150 mg/kg, i.p.) caused significant decreases in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020708603495
更新日期：1998-10-01 00:00:00
abstract：:Synaptosomal membrane proteins solubilized with 8% CHAPS-8 M urea were analyzed with two-dimensional electrophoresis (2DE). The membrane proteins were resolved up to 250 spots on a 2DE map, ranging in isoelectric points (pI) from 3.5 to 10.0 and molecular weights (MW) from 10 kDa to 200 kDa. Comparison of the mapped p...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966203
更新日期：1990-05-01 00:00:00
abstract：:Serotonin-synthesizing raphé/parapyramidal neurons (5-HT neurons) may function as sympathetic premotor neurons regulating sympathetic outflow to the cutaneous vascular bed. In the present study a genetically engineered pseudorabies virus (PRV) expressing green fluorescent protein (GFP) was injected into the rat tail. ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-9018-2
更新日期：2006-02-01 00:00:00
abstract：:Selective and limited proteolysis is a key step in the post-translational modification of peptide hormone precursors. This process appears to involve a proteolytic machinery including highly specific endoproteases. Some of the enzyme systems possibly involved in the processing of pro-neuropeptides will be described an...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00966318
更新日期：1987-10-01 00:00:00
abstract：:The role of repulsive guidance molecule A (RGMa) in cerebral ischemia remains unclear. In the study, we examined the expression of RGMa in ischemic brain tissues following focal cerebral ischemia/reperfusion (IR) in rats. An established middle cerebral artery suture occlusion model was employed. A dipolar electrode wa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0809-y
更新日期：2012-09-01 00:00:00
abstract：:The vesicular hypothesis has stimulated fruitful investigations on many secreting systems. In the case of rapid synaptic transmission, however, the hypothesis has been found difficult to reconcile with a number of well established observations. Brief impulses of transmitter molecules (quanta) are emitted from nerve te...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022451224748
更新日期：1998-05-01 00:00:00
abstract：:Parkinson's disease (PD) is the second most prevalent age-related neurodegenerative disease with physiological manifestations including tremors, bradykinesia, abnormal postural reflexes, rigidity and akinesia and pathological landmarks showing losses of dopaminergic neurons in the substantia nigra. Although the etiolo...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-008-9656-2
更新日期：2009-01-01 00:00:00
abstract：:Passive avoidance (PA) conditioning is a fear motivated task able to initiate a cascade of altered gene expression within the hippocampus, a structure critical to learning and memory. We have previously shown that neurofibromin (NF1) and amyloid precursor protein (APP), two genes implicated in cognitive function, are ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0949-0
更新日期：2013-03-01 00:00:00
abstract：:Several models for the transmission and progression of prion diseases have arisen, evolving with the acquisition of new experimental results. It is generally accepted that the PrP(Sc) protein is at least part of the infectious particle and the major protein component of the scrapie-associated fibrils (SAFs) that chara...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1023215207981
更新日期：2003-06-01 00:00:00
abstract：:We examined the impact of peroxiredoxin-I (Prx-I) and peroxiredoxin-II (Prx-II) stable transduction on oxidative stress in PC12 neurons and NIH3T3 fibroblasts and found variability depending on cell type and Prx subtype. In PC12 neurons, Prx-II suppressed reactive oxygen species (ROS) generation by 36% (p < 0.01) rela...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1026670620633
更新日期：2000-12-01 00:00:00
abstract：:Recent evidence indicates that mechanisms involved in reward and mechanisms involved in learning interact, in that reward includes learning processes and learning includes reward processes. In spite of such interactions, reward and learning represent distinct functions. In the present study, as part of an examination ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9343-8
更新日期：2007-10-01 00:00:00
abstract：:The features of neuronal damage induced by the mitochondrial toxin NaN(3) were investigated in rat primary cortical neuron cultures. Cell viability (MTT colorimetric determination) and transmembrane mitochondrial potential (J-C1 fluorescence) were concentration-dependently reduced 24 h after NaN(3); neither nuclear fr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9852-0
更新日期：2009-05-01 00:00:00
abstract：:Changes in serotonin (5-HT) and 5-hydroxy indole acetic acid (5-HIAA), its major metabolite, in cerebral cortex, corpus striatum and hippocampus were investigated at 10th and 21st days of chronic ethanol ingestion in Wistar rats. Ethanol (7.2% v/v) was given to rats in a modified liquid diet. Biochemical analysis was ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007579705230
更新日期：2000-02-01 00:00:00
abstract：:Activation of glial cells has been observed in neurodegenerative diseases including Alzheimer's disease (AD). Aggregation of amyloid β (Aβ) is profusely observed as characteristic pathology in AD brain. In our previous study using microglial cell line BV-2, tissue-type transglutaminase (TG2) was found to be involved i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2284-y
更新日期：2018-01-01 00:00:00
abstract：:Glucocorticoid receptors (GRs) exert actions on the hippocampus that are important for memory formation. There are correlations between vascular dysfunctions and GR-related gene expression. Both vascular dysfunction and GR gene expression decline occur during the ageing process. Therefore, hypotensors, which have effe...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2366-x
更新日期：2017-11-01 00:00:00
abstract：:Diurnal changes in Chordc1 mRNA were recently described in mouse hypothalamus. This report shows that Chordc1 mRNA changes rhythmically throughout the entire adult brain with highest expression levels occurring around the dark-light transition. The rhythmic cycling pattern of Chordc1 was retained under various light-d...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9271-z
更新日期：2007-02-01 00:00:00
