Abstract：
:The effects of chronic pentobarbital (PB) treatment on the binding characteristics of [3H]flunitrazepam (FLU) in rat brain were examined. Saline or sodium PB (500 micrograms/10 microliters/hr) was infused into the lateral cerebral ventricles of rats for 6 days using osmotic pumps. Immediately before withdrawal, there were no significant differences in [3H]FLU binding constants (KD and Bmax) between saline and PB groups. However, 24 hr withdrawal caused an increase in Bmax with no changes in KD. The enhancement of [3H]FLU binding by in vitro addition of chloride ions and PB was not affected after the PB infusion. The PB enhancement of [3H]FLU binding was inhibited by the convulsant, picrotoxinin. PB withdrawal did not cause significant differences in the binding constants of [3H]Ro 15-1788, a benzodiazepine (BZ) antagonist, between the saline and PB groups. Pretreatment of membranes with 0.02 mM of 3-[(3-cholamidopropyl)-dimethylammonio]-1- propanesulfonate (CHAPS), a zwitterionic detergent, caused decreases in both KD and Bmax in FLU binding in PB-withdrawal membrane, but not in the saline-treated membrane. The enhancement of [3H]FLU binding by chloride ions and PB was not affected by the CHAPS treatment. These results suggest that the change in BZ receptors induced by PB withdrawal is functionally linked to the GABA-BZ-barbiturate receptor complex and that PB withdrawal induces some conformational changes in BZ receptors.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Miyaoka T,Kimura T,Saunders PA,Tseng YT,Ho IKdoi
10.1007/BF00966726subject
Has Abstractpub_date
1994-01-01 00:00:00pages
37-42issue
1eissn
0364-3190issn
1573-6903journal_volume
19pub_type
杂志文章abstract：:Cell densities and respiratory rates of astrocytes from neonatal rat brain grown in primary culture were determined after 20-30 days in vitro. Cells grown in flasks reached lower densities (microgram DNA/cm2) and higher protein:DNA ratios than cells grown in petri dishes. Respiratory rates were lower for cells grown i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964355
更新日期：1981-12-01 00:00:00
abstract：:Several methods have been described previously for the purification of the nervous-tissue specific protein kinase C substrate B-50 (GAP-43). In this paper we present a new purification method for B-50 from rat brain which employs 2-mercaptoethanol to release the protein from isolated synaptosomal plasma membranes. Mos...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00998271
更新日期：1993-08-01 00:00:00
abstract：:Single exposure of adult male rats to low environmental temperature (LET, 12 +/- 0.5 degrees C) for 2 h significantly increased the hypothalamic and striatal GABA levels without affecting those in other regions of brain. The activity of glutamate decarboxylase (GAD) was elevated in hypothalamus (H) and corpus striatum...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968559
更新日期：1990-08-01 00:00:00
abstract：:The endogenous sialidase (N-acetylneuraminidase) activity of membranes prepared from goldfish retina and optic tectum displays characteristics similar to those reported for neural plasma membrane sialidases of other organisms. Endogenous membrane sialidase activity was found to be optimal at ph 4.0, and maximal releas...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965657
更新日期：1983-01-01 00:00:00
abstract：:There is a growing body of evidence confirming the involvement of oxidative stress and inflammation in pathogenesis of schizophrenia. Inter-individual variation in antioxidant capacity caused by different genetic profile could potentially influence patient's susceptibility to oxidative damage. In this study we evaluat...
journal_title：Neurochemical research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s11064-012-0937-4
更新日期：2013-02-01 00:00:00
abstract：:Detergents Triton X-100, sodium deoxycholate, and octyl-beta-D-glucopyranoside, and proteinase papain proved to be excellent agents solubilizing the gamma-glutamyltransferase (gamma-GT) from human brain cortex microvessels. Ficin also solubilized gamma-GT but to a lesser extent than papain. The relative molecular mass...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964524
更新日期：1984-07-01 00:00:00
abstract：:The present study was designed to evaluate the potential role of miR-93 in cerebral ischemic/reperfusion (I/R) injury in mice. The stroke model was produced in C57BL/6 J mice via middle cerebral artery occlusion (MCAO) for 1 h followed by reperfusion. And miR-93 antagomir was transfected to down-regulate the miR-93 le...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1975-0
更新日期：2016-10-01 00:00:00
abstract：:A double ligand enzyme-linked immunosorbent assay (ELISA) has been developed to detect antibodies against brain tissue antigens in the sera of patients with neurological diseases. The sera were tested on human white matter homogenate. The technique consists of successive incubations with the human serum to be tested, ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964651
更新日期：1985-04-01 00:00:00
abstract：:Changes in serotonin (5-HT) and 5-hydroxy indole acetic acid (5-HIAA), its major metabolite, in cerebral cortex, corpus striatum and hippocampus were investigated at 10th and 21st days of chronic ethanol ingestion in Wistar rats. Ethanol (7.2% v/v) was given to rats in a modified liquid diet. Biochemical analysis was ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007579705230
更新日期：2000-02-01 00:00:00
abstract：:Although nitric oxide (NO) plays key signaling roles in the nervous systems, excess NO leads to cell death. In this study, the involvement of p38 mitogen-activated protein kinase (p38 MAPK) and apoptosis signal-regulating kinase-1 (ASK1) in NO-induced cell death was investigated in PC12 cells. NO donor transiently act...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1010917129951
更新日期：2001-05-01 00:00:00
abstract：:Memory impairment is a major problem afflicting mankind. The association between memory functions and neurotransmitter functions is of great interest for understanding brain function. Serotonergic pathways play an important role in the modulation of memory functions but the importance of its receptor types and subtype...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0839-5
更新日期：2012-10-01 00:00:00
abstract：:The expression and localization of syntaxin isoforms 1A and 1B in adrenergic and noradrenergic chromaffin cells were examined by both immunoblot analysis and confocal immunofluorescence microscopy. Syntaxin 1A was found in higher levels in noradrenergic cells, whereas syntaxin 1B was similarly expressed in most noradr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1025658005937
更新日期：2003-10-01 00:00:00
abstract：:The aim of this study was to determine the anticancer potential of Leonurus sibiricus extract derived from in vitro transgenic roots transformed by Agrobacetrium rhizogenes with AtPAP1 transcriptional factor, and that of transformed roots without construct, on grade IV human glioma cells and the U87MG cell line, and a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2551-6
更新日期：2018-07-01 00:00:00
abstract：:Zinc oxide nanoparticles (ZnO NPs) have diverse utility these days ranging from being part of nanosensors to be ingredient of cosmetics. Present study was designed to report the effect of variable doses of ZnO NPs on selected aspects of male albino mice behavior. Nano particles were synthesized by sol-gel auto-combust...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-2090-y
更新日期：2017-02-01 00:00:00
abstract：:Oxidative stress-induced neuronal cell death requires opening of the mitochondrial permeability transition pore. P53 mitochondrial translocation and association with Cyclophilin D (Cyp-D) is required for the pore opening. Here we tested this signaling axis in oxygen glucose deprivation (OGD)/re-oxygenation-induced in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-0968-5
更新日期：2013-04-01 00:00:00
abstract：:The vesicular hypothesis has stimulated fruitful investigations on many secreting systems. In the case of rapid synaptic transmission, however, the hypothesis has been found difficult to reconcile with a number of well established observations. Brief impulses of transmitter molecules (quanta) are emitted from nerve te...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022451224748
更新日期：1998-05-01 00:00:00
abstract：:Lipid synthesis from acetoacetate and 3-hydroxybutyrate was studied in chick embryo from 15 to 21 days and in chick neonate from 1 to 21 days. Embryonic spinal cord showed higher ability than brain to incorporate acetoacetate into total lipids, although a sharp decrease was found at hatching. 3-Hydroxybutyrate incorpo...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966692
更新日期：1993-10-01 00:00:00
abstract：:This study was designed to investigate the effects of chronic toluene inhalation in high concentration on lipid peroxidation, antioxidant enzyme activities and ultrastructural changes in the sciatic nerves of rats. Male Wistar albino rats (150-250 g) were divided in two experimental groups: the control and the toluene...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-9683-6
更新日期：2005-01-01 00:00:00
abstract：:The NG 108-15 (neuroblastoma X glioma hybrid) cell line was used as an in vitro neuronal model to evaluate potential antagonists of the Na+-selective carboxylic ionophore monensin. Changes in membrane electrical characteristics induced by monensin with and without the simultaneous administration of antagonists were me...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007548307554
更新日期：2000-07-01 00:00:00
abstract：:Anesthetics such as propofol can provide neuroprotective effects against cerebral ischemia. However, the underlying mechanism of this beneficial effect is not clear. Therefore, we subjected male Sprague-Dawley rats to 2 h of middle cerebral artery occlusion and investigated how post-ischemic administration of propofol...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0945-4
更新日期：2013-03-01 00:00:00
abstract：:Evidence has suggested that cerebrospinal fluid-contacting nucleus (CSF-contacting nucleus) is correlated with the development and recurrence of pain. A recent research showed that the CSF-contacting nucleus acts as a component of the descending 5-hydroxytryptamine (5-HT) system and plays a role in descending pain inh...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1880-6
更新日期：2016-07-01 00:00:00
abstract：:In our previous study vesamicol, an inhibitor of the acetylcholine transporter of the cholinergic vesicles, inhibited veratridine-evoked external Ca(2+)-dependent acetylcholine release from striatal slices but did not influence acetylcholine release observed in Ca(2+)-free medium (4). Here we examined if the effect of...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968780
更新日期：1992-06-01 00:00:00
abstract：:The maximal rate of some cerebral enzymatic activities related to energy transduction (hexokinase; phosphofructokinase; lactate dehydrogenase; citrate synthase; malate dehydrogenase; total NADH-cytochrome c reductase; cytochrome oxidase), amino acid metabolism (glutamate decarboxylase; glutamate dehydrogenase) and cho...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963926
更新日期：1983-02-01 00:00:00
abstract：:Mevalonate phosphorylation was studied in neonatal chick brain. Formation of phosphorylated derivatives of mevalonic acid increased with the pH in the range assays (5.5-9.5). Phosphomevalonate kinase was completely inactivated after treatment at 50 degree C for 5-10 min, whereas mevalonate kinase was found to retain i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965047
更新日期：1981-08-01 00:00:00
abstract：:Long-term intake of aspartame at the acceptable daily dose causes oxidative stress in rodent brain mainly due to the dysregulation of glutathione (GSH) homeostasis. N-Acetylcysteine provides the cysteine that is required for the production of GSH, being effective in treating disorders associated with oxidative stress....
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1360-9
更新日期：2014-09-01 00:00:00
abstract：:Catecholamine secretion in the bovine adrenal medulla is evoked largely by nicotinic receptor activation. However, bovine adrenal medulla also contain muscarinic receptors that mediate several cell responses. To understand the physiological role of muscarinic receptors in the bovine adrenal medulla it is important to ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968406
更新日期：1992-12-01 00:00:00
abstract：:The recent publication of the human genome sequence provides an opportunity both to combat diseases that are presently considered as pharmaceutically intractable and also to improve current therapies for many common human diseases. The identification of every human gene by ongoing bioinformatic efforts has the potenti...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022349805831
更新日期：2003-02-01 00:00:00
abstract：:The pineal gland is a neuroendocrine gland responsible for nocturnal synthesis of melatonin. During early development of the rodent pineal gland from the roof of the diencephalon, homeobox genes of the orthodenticle homeobox (Otx)- and paired box (Pax)-families are expressed and are essential for normal pineal develop...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-012-0906-y
更新日期：2013-06-01 00:00:00
abstract：:Using the replication-defective adenovirus vector, we overexpressed rat glia maturation factor (GMF) in primary astrocyte cultures derived from embryonic rat brains. Among the three isoforms of MAP kinase, there was a big increase in the phosphorylation of p38, as detected with Western blotting using the phosphospecif...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1014241300179
更新日期：2001-12-01 00:00:00
abstract：:The Editors have retracted this article [1] following an investigation conducted by the journal. After publication concerns were raised regarding interpretation of the data presented in Fig. 4. The Editors requested additional data and clarification to confirm interpretation of data results. After further review, the ...
journal_title：Neurochemical research
pub_type： 杂志文章,撤回出版物
doi：10.1007/s11064-020-03077-0
更新日期：2020-09-01 00:00:00
