Abstract：
:Cell densities and respiratory rates of astrocytes from neonatal rat brain grown in primary culture were determined after 20-30 days in vitro. Cells grown in flasks reached lower densities (microgram DNA/cm2) and higher protein:DNA ratios than cells grown in petri dishes. Respiratory rates were lower for cells grown in flasks compared to cells grown in dishes. The pH of the medium in flasks fell below 6.9 between feedings while the pH of the medium in dishes remained at about 7.2. Cells grown in dishes with the medium pH adjusted to 6.8 also showed lower final cell densities, higher protein:DNA ratios, and lower respiratory rates, compared to cells grown under similar conditions at pH 7.5. Intermediate values of each parameter were found in cells grown at pH 7.5 for one week and then at 6.8 for 20 days. We conclude that the effects of ambient pH account for the differences in growth characteristics and respiratory rates of astrocytes grown in dishes versus those grown in flasks.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Olson JE,Holtzman Ddoi
10.1007/BF00964355subject
Has Abstractpub_date
1981-12-01 00:00:00pages
1337-43issue
12eissn
0364-3190issn
1573-6903journal_volume
6pub_type
杂志文章abstract：:DYT1 dystonia is caused by a glutamic acid deletion (ΔE) in the endoplasmic reticulum (ER) protein torsinA. Previous studies suggest that torsinA modulates the aggregation of cytosolic misfolded proteins and ER stress responses, although the mechanisms underlying those effects remain unclear. In order to investigate t...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0363-4
更新日期：2011-03-01 00:00:00
abstract：:The present study was designed to evaluate the potential role of miR-93 in cerebral ischemic/reperfusion (I/R) injury in mice. The stroke model was produced in C57BL/6 J mice via middle cerebral artery occlusion (MCAO) for 1 h followed by reperfusion. And miR-93 antagomir was transfected to down-regulate the miR-93 le...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1975-0
更新日期：2016-10-01 00:00:00
abstract：:Calcium-naive synaptosomes were used to assess the effects of divalent cations on [3H]acetylcholine release from rat hippocampal homogenates. Following equilibration with calcium-free buffer (containing 10 microM EGTA), calcium reversibly increased [3H]acetylcholine efflux (up to five-fold) while causing no measurable...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966598
更新日期：1991-10-01 00:00:00
abstract：:Precise control of the proliferation and differentiation of multipotent neural stem cells (NSCs) is crucial for the proper development of the nervous system. Although cyclinD1 has been implicated as a cause of cancer in many studies, its roles in NSCs remain elusive. In this study, we examined the over-expression of c...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1635-9
更新日期：2015-08-01 00:00:00
abstract：:Neuropeptides are derived from large and inactive proteins which require endoproteolytic processing for the biosynthesis of the bioactive peptides. The maturation of pro-neuropeptide to neuropeptide is believed to be performed by ortholog pro-protein convertase EGL-3 in Caenorhabditis elegans (C. elegans). Furthermore...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2636-2
更新日期：2018-11-01 00:00:00
abstract：:The release of labeled norepinephrine from preloaded synaptosomes requires the presence of potassium and calcium. ATP-dependent binding of calcium to synaptic plasma membranes (SPM) may provide a means of maintaining the cation in a readily available pool for the triggering of transmitter release. A high Ca-binding ca...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965106
更新日期：1983-04-01 00:00:00
abstract：:We have previously showed that peptide neurotensin inhibits neuronal Na(+), K(+)-ATPase activity, an effect which involves high affinity neurotensin receptor. Nitric oxide (NO) acts as a neurotransmitter or as a neuromodulator when it is synthesized by neuronal nitric oxide synthase. Neurotensin effect on Na(+), K(+)-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0552-9
更新日期：2011-12-01 00:00:00
abstract：:Brief limb ischemia was reported to protect neurons against injury induced by subsequent cerebral ischemia-reperfusion, and this phenomenon is known as limb ischemic preconditioning (LIP). To explore the role of nitric oxide (NO) in neuroprotection of LIP in rats, we observed changes in the content of nitric oxide (NO...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9381-2
更新日期：2007-11-01 00:00:00
abstract：:We investigated the distribution of serotonin (5-HT) receptors of type 3 (5-HT3) in human brain areas, by means of the the specific binding of [3H]GR65630. The brains were obtained during autoptic sessions from 6 subjects. Human brain membranes and the binding of [3H]GR65630 were carried out according to standardized ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1010939530412
更新日期：2001-03-01 00:00:00
abstract：:Parecoxib is a recently described novel COX-2 inhibitor whose functional significance and neuroprotective mechanisms remain elusive. Therefore, in this study, we aimed to investigate whether delayed administration of parecoxib inhibited mitochondria-mediated neuronal apoptosis induced by ischemic reperfusion injury vi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0615-y
更新日期：2012-02-01 00:00:00
abstract：:The control and maintenance of the intracellular redox state is an essential task for cells and organisms. NAD(+) and NADH constitute a redox pair crucially involved in cellular metabolism as a cofactor for many dehydrogenases. In addition, NAD(+) is used as a substrate independent of its redox-carrier function by enz...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-012-0760-y
更新日期：2012-11-01 00:00:00
abstract：:It has been reported that central dopaminergic system is implicated in the mechanism underlying general anesthesia. Whether dopamine (DA) neurons in midbrain ventral periaqueductal gray (vPAG) are involved in general anesthesia and how general anesthetics affect these neurons remain sparsely documented. To determine t...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2486-y
更新日期：2018-04-01 00:00:00
abstract：:Cell surface β-Amyloid precursor protein (APP) is known to have a functional role in iron homeostasis through stabilising the iron export protein ferroportin (FPN). Mechanistic evidence of this role has previously only been provided through transcriptional or translational depletion of total APP levels. However, numer...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02747-y
更新日期：2019-06-01 00:00:00
abstract：:Glutamate dehydrogenase (GDH) is a homohexameric enzyme that catalyzes the reversible oxidative deamination of L-glutamate to 2-oxoglutarate. Only in the animal kingdom is this enzyme heavily allosterically regulated by a wide array of metabolites. The major activators are ADP and leucine and inhibitors include GTP, p...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-013-1173-2
更新日期：2014-01-01 00:00:00
abstract：:It is thought that changes in gene expression in the brain mediate chronic ethanol-induced complex behaviors such as tolerance, dependence, and sensitization, and also relate to ethanol-induced brain toxicity. Using high-density filter-based cDNA microarrays (GeneFilters), we analyzed the expression of over 5000 genes...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020937728506
更新日期：2002-10-01 00:00:00
abstract：:The effect of the calcium channel blocker (KB-2796) on metabolic and functional recovery in rabbit spinal cord after 20, 30, and 40 min ischemia and 4 days of recovery was investigated. The drug was given intraperitoneally in three different doses, 10, 20, or 50 mg/kg pre- or post-ischemia of 20, 30, or 40 min duratio...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968997
更新日期：1994-12-01 00:00:00
abstract：:Zonisamide (ZNS), an antiepileptic drug having beneficial effects also against Parkinson's disease symptoms, has proven to display an antioxidant effects in different experimental models. In the present study, the effects of ZNS on rotenone-induced cell injury were investigated in human neuroblastoma SH-SY5Y cells dif...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1181-2
更新日期：2013-12-01 00:00:00
abstract：:Post-ischemic changes in energy metabolites and natural antioxidant compounds have been measured in rat brain in vitro concurrent with two different assays for peroxidized lipids. No exogenous free radical initiators were employed. In vitro oxygenation of minced brain preparations for periods of 10 minutes to 4 hours,...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965496
更新日期：1982-04-01 00:00:00
abstract：:Hypothalamic and plasma dopamine-beta-hydroxylase [DBH, EC 1.14.2.1] activites were measured by a coupled radioenzymatic method. Animals representing five experimental groups [intact controls, adrenalectomized, pinealectomized, adrenalectomized pinealectomized, doubly sham-operated] were killed and sampled at 8 times ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965575
更新日期：1978-06-01 00:00:00
abstract：:Convulsant doses of penicillin and elevated ambient pressure of 41 bar enhance the excitability of neurons. Their effects have been studied in neostriatal tissue with methods allowing differentiation between transmitter and metabolic glutamate pools. Levels of glutamate (Glu), glutamine (Gln), aspartate (Asp); gamma-a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965621
更新日期：1989-11-01 00:00:00
abstract：:Mammalian MTH1 protein, a MutT-related protein, catalyzes the hydrolysis of 8-oxo-7,8-dihydroguanosine triphosphate (8-oxoGTP) to monophosphate, thereby preventing incorporation of 8-oxo-7,8-dihydroguanine (8-oxoguanine) into RNA. In this study, we applied immunohistochemistry to follow the expression of MTH1 and the ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0484-4
更新日期：2011-08-01 00:00:00
abstract：:Chicken neurons in culture display measurable activities of mono- and diacylglycerol lipases. Treatment of chicken neuronal cultures with gangliosides (10(-8)M to 10(-5)M) resulted in a time and dose dependent increase in monoacylglycerol lipase activity. The diacylglycerol lipase showed significant increase in specif...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966702
更新日期：1991-11-01 00:00:00
abstract：:In this study, changes of the expression of two mitochondrial and two nuclear genes encoding the subunits of cytochrome c oxidase (CO) and NADH dehydrogenase (ND) were studied in the hippocampus, inferior parietal lobule, and cerebellum of 10 Alzheimer's disease (AD) and 10 age-matched control subjects. The altered pr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020783614031
更新日期：1999-06-01 00:00:00
abstract：:Metabolic modeling of dynamic (13)C labeling curves during infusion of (13)C-labeled substrates allows quantitative measurements of metabolic rates in vivo. However metabolic modeling studies performed in the brain to date have only modeled time courses of total isotopic enrichment at individual carbon positions (posi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0782-5
更新日期：2012-11-01 00:00:00
abstract：:Adaptive changes in serotonergic 5HT1 receptor signalling are believed to underlie the therapeutic effectiveness of antidepressant drugs. Since cells are continuously exposed to neurotransmitters/neuromodulators, spatially and temporally integrated, the responsiveness of a receptor system is dependent upon the physio-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9582-8
更新日期：2008-07-01 00:00:00
abstract：:In this paper we propose a powerful procedure to measure functional activation of the mouse delta-opioid receptor transiently expressed in mammalian cells. Receptor stimulation was assessed using a population of electroporated COS cells, transfected at a 50% efficiency. Under those conditions, agonist-promoted activat...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02532371
更新日期：1996-11-01 00:00:00
abstract：:Primary malignant brain cancer, one of the most deadly diseases, has a high rate of recurrence after treatment. Studies in the past several years have led to the hypothesis that the root of the recurrence may be brain tumor stem cells (BTSCs), stem-like subpopulation of cells that are responsible for propagating the t...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-009-0079-5
更新日期：2009-12-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common form of dementia that progressively disrupts neurocognitive function, which has neither cure nor effective treatment. Hypercholesterolemia might be involved in brain alterations that could evolve into AD. The present study aims to evaluate the potential of omega-3, Co-enzyme...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-02996-2
更新日期：2020-05-01 00:00:00
abstract：:Any spinal cord injury carries the potential for persistent disability affecting motor, sensory and autonomic functions. To prevent this outcome, it is highly desirable to block a chain of deleterious reactions developing in the spinal areas immediately around the primary lesion. Thus, early timing of pharmacological ...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2459-6
更新日期：2019-01-01 00:00:00
abstract：:Metabolic turnover of axonally transported glycoproteins has been examined in membranous and soluble subfractions of goldfish optic tectum following intraocular injection of [3H]fucose. Radioactivity in total transported glycoproteins reached a maximum in the tectum after 24-30 hr, then declined with a half-life of ap...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964224
更新日期：1980-04-01 00:00:00
