Abstract：
:Post-ischemic changes in energy metabolites and natural antioxidant compounds have been measured in rat brain in vitro concurrent with two different assays for peroxidized lipids. No exogenous free radical initiators were employed. In vitro oxygenation of minced brain preparations for periods of 10 minutes to 4 hours, following 5 minutes of preparatory ischemia, yielded increased levels of lipid conjugated dienes and TBA-reactive material, in contrast to anaerobically incubated preparations. However, either aerobic or anaerobic incubation of brain minces facilitated increased ratios of lactate:pyruvate and glutathione (oxidized):glutathione (reduced), as well as increased total ubiquinone content and loss of alpha-tocopherol. Observation of lipid radical formation in vivo was then attempted using rats given embolic stroke in one hemisphere and left in the post-ischemic condition for times up to 24 hours. Conjugated dienes were found in lipids extracted from the ipsilateral hemisphere but not from the contralateral hemisphere. These observations of conjugated dienes in vivo (formed presumably during post-ischemic reperfusion) and in vitro (facilitated by oxygenation of brain minces), indicate that lipid radical intermediates and associated chain peroxidation processes are potentiated by ischemia and occur during tissue reoxygenation.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Kogure K,Watson BD,Busto R,Abe Kdoi
10.1007/BF00965496subject
Has Abstractpub_date
1982-04-01 00:00:00pages
437-54issue
4eissn
0364-3190issn
1573-6903journal_volume
7pub_type
杂志文章abstract：:Harpagophytum procumbens, popularly known as devil's claw, is a plant commonly used in the treatment of diseases of inflammatory origin. The anti-inflammatory effects of H. procumbens have been studied; however, the mechanism of action is not elucidated. It is known that excess of reactive oxygen and nitrogen species ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1133-x
更新日期：2013-11-01 00:00:00
abstract：:The acetylcholinesterase from synaptosomal membranes is inhibited by anesthetics: Nembutal, brietal, and thiopental. Nembutal and brietal decrease the Km for acetylthiocholine, without changes in Vmas. A noncompetitive type of inhibition is produced by thiopental. This anesthetic decreases Arrhenius plot discontinuity...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964714
更新日期：1980-07-01 00:00:00
abstract：:Biotinylated derivatives of tetanus toxin were prepared and isolated by chromatofocusing and ganglioside-affinity chromatography. Biotinylation was monitored by the appearance of a 210,00 dalton complex upon SDS-polyacrylamide gel electrophoresis in the presence of avidin, and by selective binding to an avidin-Sepharo...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969922
更新日期：1990-04-01 00:00:00
abstract：:There is increasing evidence to suggest that hepatic encephalopathy in acute liver failure is the result of altered glutamatergic function. In particular, the high affinity uptake of glutamate is decreased in brain slices and synaptosomes from rats with acute liver failure as well as by exposure of cultured astrocytes...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022532623281
更新日期：1999-11-01 00:00:00
abstract：:The release of the inhibitory neurotransmitter GABA is generally enhanced under potentially cell-damaging conditions. The properties and regulation of preloaded [3H]GABA release from mouse hippocampal slices were now studied in free radical-containing medium in a superfusion system. Free radical production was induced...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9439-1
更新日期：2008-03-01 00:00:00
abstract：:Evidence is presented in support of the working hypothesis that "prenervous" neurotransmitters directly participate in cell-cell interactions occurring during the first several cleavage divisions of sea urchin embryos, a function which may occur during the early development of higher animals as well. This intercellula...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963906
更新日期：1981-01-01 00:00:00
abstract：:Rhesus macaque monkey brain microvessel endothelial cells (BMECs) were isolated and grown in culture in an effort to establish an appropriate primate in vitro model of the endothelial component of the blood-brain barrier. The presence of Factor VIII antigen, alkaline phosphatase, gamma-glutamyl transpeptidase, lactate...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00967320
更新日期：1994-04-01 00:00:00
abstract：:Factors secreted by C6 glioma cells which induce electrical resistances across endothelial monolayers in an in vitro blood-brain barrier model have been partially characterised for the first time. These transendothelial electrical resistances (TEERs) were only evident when cell-free conditioned medium derived from C6 ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020932121378
更新日期：1998-12-01 00:00:00
abstract：:Deposition of amyloid beta peptide in human brain in the form of senile plaques is a neuropathological hallmark of Alzheimer's disease (AD). Levels of a phospholipid breakdown product, glycerophosphocholine (GPC), also increase in AD brain. The effect of GPC on amyloid beta(1-40) peptide (Abeta) aggregation in PBS buf...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-7036-0
更新日期：2004-12-01 00:00:00
abstract：:Human dental follicle cells (DFCs) are ectomesenchymal multipotent stem cells that form spheroid cell clusters (SCCs) under serum free medium cell culture conditions (SFM). Until today, molecular mechanisms for the formation of SCCs are unknown. In this study a quantitative phosphoproteomics approach revealed regulate...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1298-y
更新日期：2014-07-01 00:00:00
abstract：:Sonic hedgehog (Shh), a secreted glycoprotein factor, can activate the Shh pathway, which has been implicated in neuronal polarization involving neurite outgrowth. However, little evidence is available about the effect of Shh on neurite outgrowth in primary cortical neurons and its potential mechanism. Here, we reveal...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1736-5
更新日期：2016-04-01 00:00:00
abstract：:Oxidative stress-induced neuronal cell death requires opening of the mitochondrial permeability transition pore. P53 mitochondrial translocation and association with Cyclophilin D (Cyp-D) is required for the pore opening. Here we tested this signaling axis in oxygen glucose deprivation (OGD)/re-oxygenation-induced in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-0968-5
更新日期：2013-04-01 00:00:00
abstract：:An in vitro procedure for labeling of RNA in the excised rat nodose ganglion was used to evaluate the changes in incorporation of [3H]uridine into ganglionic RNA following transection of the abdominal vagus nerves. Significant increases in the incorporation into 28S, 18S and 4S RNA were observed at 1 day after injury,...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965726
更新日期：1983-03-01 00:00:00
abstract：:Although nitric oxide (NO) plays key signaling roles in the nervous systems, excess NO leads to cell death. In this study, the involvement of p38 mitogen-activated protein kinase (p38 MAPK) and apoptosis signal-regulating kinase-1 (ASK1) in NO-induced cell death was investigated in PC12 cells. NO donor transiently act...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1010917129951
更新日期：2001-05-01 00:00:00
abstract：:DNase I sensitivity experiments were performed utilizing DNA probes to genes which are either transcribed in rat cortical neurons (the 68 kDa neurofilament gene and the neuron-specific enolase gene) or are transcriptionally silent (albumin). Results suggest that unlike liver, in which a hierarchy in chromatin conforma...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969627
更新日期：1989-02-01 00:00:00
abstract：:A role for protein phosphorylation in the process of neurite outgrowth has been inferred from many studies of the effects of protein kinase inhibitors and activators on cultured neurotumor cells and primary neuronal cells from developing brain or ganglia. Here we re-examine this issue, using a culture system derived f...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021916509858
更新日期：1997-10-01 00:00:00
abstract：:Glutamate and its receptors are expressed very early during development and may play important roles in neurogenesis, synapse formation and brain wiring. The levels of glutamate and activity of its receptors can be influenced by exogenous factors, leading to neurodevelopmental disorders. To investigate the role of NMD...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9213-9
更新日期：2007-02-01 00:00:00
abstract：:Most studies of Alzheimer's disease (AD) have focused on a single precipitating alteration as the etiological event rather than global changes closely linked to aging. Recent evidence suggests that the most significant of these global changes are metabolic. Here we present data indicating that metabolic rate, nutritio...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1025678510480
更新日期：2003-10-01 00:00:00
abstract：:The cyclic GMP analog 8-bromo-cyclic GMP (8-Br-cGMP) protects differentiated murine oligodendrocytes (OLs) from caspase-mediated death initiated by staurosporine, thapsigargin or kainate. Caspase-independent death caused by high levels of NO is also partially prevented by 8-Br-cGMP. Inhibitors of protein kinase G (cGM...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9187-7
更新日期：2007-02-01 00:00:00
abstract：:In this paper, we show that caveolin-1 is abundantly present in a cell line of immortalized gonadotropin-releasing hormone-expressing neurons (GN11). In contrast to GN11, caveolin is undetectable in a cognate cell line of immortalized gonadotropin-releasing hormone-secreting neurons (GT1-7). These two cell lines are c...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020217309987
更新日期：2002-08-01 00:00:00
abstract：:Changes in serotonin (5-HT) and 5-hydroxy indole acetic acid (5-HIAA), its major metabolite, in cerebral cortex, corpus striatum and hippocampus were investigated at 10th and 21st days of chronic ethanol ingestion in Wistar rats. Ethanol (7.2% v/v) was given to rats in a modified liquid diet. Biochemical analysis was ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007579705230
更新日期：2000-02-01 00:00:00
abstract：:N-methyl-D-aspartate (NMDA) inhibits carbachol-stimulated phosphoinositide breakdown in rat brain cortical slices but not in isolated membranes (1). To gain insight into the mechanisms, we examined the effects of NMDA on carbachol-stimulated [3H]inositol phosphate and intermediates of phosphatidylinositol cycle accumu...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022426204583
更新日期：1997-05-01 00:00:00
abstract：:Microdissection of selected regions of central nervous system (CNS) has provided the basis of modern chemoarchitectonics. Laser microdissection is a modern variant of the "Palkovits punch" technique and used together with gene array analysis has revolutionalized CNS molecular analysis. Here we describe the use of such...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-9008-4
更新日期：2006-02-01 00:00:00
abstract：:Docosahexaenoic acid (DHA) is verified to have neuroprotective effects on traumatic brain injury (TBI) rats by activating Nrf2 signaling pathway, but the role of NOX2 in this effect has not been illuminated. So this study explored the role of NOX2 in TBI models treated with DHA, aiming to complete the mechanism of DHA...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03078-z
更新日期：2020-08-01 00:00:00
abstract：:The kinetics of the uptake of p-tyramine, m-tyramine, and dopamine were investigated in slices of the hypothalamus and striatum of the rat in the presence of nialamide. When uptake was analyzed by a least-squares fit to a Lineweaver-Burk plot, each amine appeared to be concentrated by both a "low"-affinity and a "high...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964440
更新日期：1979-10-01 00:00:00
abstract：:Consumption of fifteen percent alcohol, during gestation did not cause any decrease in the total calorie or fluid intake of the rats maintained on normal dietary regimen. However, the alcohol consumption by gestating mothers resulted in a decreased contents of both DNA and protein in the CNS of the in utero alcohol ex...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965614
更新日期：1989-11-01 00:00:00
abstract：:Chronic thiamine deprivation in the rat leads to ataxia, loss of righting reflex and neuropathological damage to lateral vestibular nucleus. Before onset of neurological symptoms, transketolase (TK) activities were found to be selectively reduced by 25% in lateral vestibular nucleus and surrounding pons. Further progr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00972141
更新日期：1987-03-01 00:00:00
abstract：:Isorhynchophylline (IRN), an oxindole alkaloid, has been identified as the main active ingredient responsible for the biological activities of Uncaria rhynchophylla (Miq) Miq ex Havil. (Rubiaceae). Previous studies in our laboratory have revealed that IRN possesses potent neuroprotective effects in different models of...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-2124-5
更新日期：2017-02-01 00:00:00
abstract：:Antipsychotics represent high affinity for sigma receptors and sigma-like drugs often have the psychotomimetic properties. Besides, the receptors are unevenly distributed in human brain. These findings suggest that sigma receptors might be involved in the pathophysiology of schizophrenia. Sigma receptors in rat and hu...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966825
更新日期：1992-10-01 00:00:00
abstract：:Neurons, purified by bulk-isolation procedures from 10 day-old rat brain, are 80-90% homogeneous. Contaminants are primarily blood vessels and occasional oligodendroglia. All sizes and shapes of neurons are obtained, as they are isolated from all areas of the brain, except the cerebellum. Neurotransmitter enzyme activ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00971368
更新日期：1987-01-01 00:00:00
