Abstract：
:The role of repulsive guidance molecule A (RGMa) in cerebral ischemia remains unclear. In the study, we examined the expression of RGMa in ischemic brain tissues following focal cerebral ischemia/reperfusion (IR) in rats. An established middle cerebral artery suture occlusion model was employed. A dipolar electrode was placed into the cerebellum to stimulate the cerebellar fastigial nucleus for 1 h at 2 h after ischemia. Reverse transcription-polymerase chain reaction was used to measure the mRNA RGMa and its downstream mediator, Ras homolog A (RhoA). Immunohistochemistry was applied to detect RGMa and RhoA expressions and to evaluate axonal regeneration by optical density analysis of 200 kDa neurofilaments. We found that both mRNA and protein levels of RGMa and RhoA were increased in the ischemic cortex and hippocampus 48 h following cerebral IR and these elevated levels were maintained for 2 weeks. Electrostimulation of the fastigial nucleus reduced the expression of RGMa and RhoA at 24 h and 2 weeks following cerebral IR. In addition, axonal growth was enhanced in the fastigial nucleus electrostimulated group compared to non-stimulated ischemic animals (P < 0.05). RGMa/RhoA expression was negatively correlated with the growth of axons (P < 0.05). Therefore, we concluded that RGMa and RhoA could be another key molecule and might inhibit axonal regeneration during cerebral IR injury. Electrostimulation of the fastigial nucleus enhances axonal growth, possibly by reducing the expression of RGMa and RhoA after cerebral IR.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Jiang F,Yin H,Qin Xdoi
10.1007/s11064-012-0809-ysubject
Has Abstractpub_date
2012-09-01 00:00:00pages
1906-14issue
9eissn
0364-3190issn
1573-6903journal_volume
37pub_type
杂志文章abstract：:Glutamine synthetase (GS) is the major glutamine-forming enzyme of vertebrates and is accepted to be a marker of astroglial cells. Maturation of astroglial cells is characterized by an increase of GS activity, and the regulation of this enzyme is the topic of many publications. Because of the fundamental role of the G...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-2754-5
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doi：10.1007/s11064-007-9287-z
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964958
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
doi：10.1007/BF00964417
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更新日期：2016-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2006-11-01 00:00:00
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更新日期：1995-01-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2354-1
更新日期：2017-11-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2008-10-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02532371
更新日期：1996-11-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
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更新日期：2014-09-01 00:00:00
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journal_title：Neurochemical research
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journal_title：Neurochemical research
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abstract：:The role of protein phosphorylation in catecholamine secretion from bovine adrenomedullary chromaffin cells was studied using different protein kinase inhibitors. Naphthalenesulfonamide derivatives as ML9 and ML7, more specific for the myosin light chain kinase, and the calmodulin antagonist W7 inhibited catecholamine...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969089
更新日期：1993-03-01 00:00:00
