Abstract:
:The JNKs have been implicated in a variety of biological functions in mammalian cells, including apoptosis and the responses to stress. However, the physiological role of these pathways in the intracerebral hemorrhage (ICH) has not been fully elucidated. In this study, we identified a MAPK kinase kinase (MAPKKK), MEKK1, may be involved in neuronal apoptosis in the processes of ICH through the activation of JNKs. From the results of western blot, immunohistochemistry and immunofluorescence, we obtained a significant up-regulation of MEKK1 in neurons adjacent to the hematoma following ICH. Increasing MEKK1 level was found to be accompanied with the up-regulation of p-JNK 3, p53, and c-jun. Besides, MEKK1 co-localized well with p-JNK in neurons, indicating its potential role in neuronal apoptosis. What's more, our in vitro study, using MEKK1 siRNA interference in PC12 cells, further confirmed that MEKK1 might exert its pro-apoptotic function on neuronal apoptosis through extrinsic pathway. Thus, MEKK1 may play a role in promoting the brain damage following ICH.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Lu H,Ning X,Tao X,Ren J,Song X,Tao W,Zhu L,Han L,Tao T,Yang Jdoi
10.1007/s11064-016-2063-1subject
Has Abstractpub_date
2016-12-01 00:00:00pages
3308-3321issue
12eissn
0364-3190issn
1573-6903pii
10.1007/s11064-016-2063-1journal_volume
41pub_type
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