Abstract:
:Activities toward arachidonyl-labelled phospholipase A2 substrates were assayed in fractions of white matter and cerebral cortex from control subjects and in fractions of demyelinated plaque, normal-appearing white matter and cerebral cortex from subjects who died with multiple sclerosis. Membranous activity at pH 8.6 in the presence of Ca2+, characteristic of 14 kDa "secretory" phospholipase A2, in either multiple sclerosis white matter or cortex did not differ from controls, whereas membranous activity at pH 4.5 in the absence of added Ca2+, characteristic of lysosomal enzymes was increased over controls in both plaque and normal-appearing white matter but not cerebral cortex. Activity in the cytosol fraction, at pH 8.6 in the presence of Ca2+ and glycerol characteristic of the "cytosolic" 85 kDa enzyme was decreased by greater than 50% in both white matter and cortex samples from multiple sclerosis subjects. Immuno-precipitation and -blotting confirmed that the deficient activity was largely attributable to the 85 kDa enzyme although the enzyme protein was not similarly reduced.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Huterer SJ,Tourtellotte WW,Wherrett JRdoi
10.1007/BF00992509subject
Has Abstractpub_date
1995-11-01 00:00:00pages
1335-43issue
11eissn
0364-3190issn
1573-6903journal_volume
20pub_type
杂志文章abstract::The copper-transporting ATPases Atp7A and Atp7B play a major role in controlling intracellular copper levels. In addition, they are believed to deliver copper to the copper-requiring proteins destined for the secretory vesicles. One cuproprotein, dopamine beta-hydroxylase (DBH) functions in the biosynthesis of norepin...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1023219308890
更新日期:2003-06-01 00:00:00
abstract::A simple, rapid method for determining depolarization-induced 45Ca influx into synaptosomes is described. Synaptosomes which had been depolarized in the presence of 45Ca were applied to a small column of Chelex-100 resin to separate free Ca2+ from that taken up by the tissue. Approximately 70% of the synaptosomal prot...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965062
更新日期:1982-02-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0866-2
更新日期:2012-12-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1007541020736
更新日期:2000-03-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2170-z
更新日期:2017-05-01 00:00:00
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更新日期:1999-10-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章,已发布勘误
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更新日期:2018-02-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章,随机对照试验
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更新日期:2013-02-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
abstract::The ketone body, β-hydroxybutyrate (βOHB), is metabolised by the brain alongside the mandatory brain fuel glucose. To examine the extent and circumstances by which βOHB can supplement glucose metabolism, we studied guinea pig cortical brain slices using increasing concentrations of [U-13C]D-βOHB in conjunction with [1...
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更新日期:2017-06-01 00:00:00
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更新日期:2000-06-01 00:00:00
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journal_title:Neurochemical research
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abstract::The vanilloid receptor 1(VR1) is a nonselective cation channel that is activated by pungent vanilloid compound, extracellular protons, or noxious heat. mRNA of VR1 and vanilloid receptor 1-like receptor (VRL1) were expressed in PC12 cells, and only VRI mRNA was detected in glioma and A10 cell lines. VRI protein was de...
journal_title:Neurochemical research
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更新日期:2004-04-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1717-8
更新日期:2015-11-01 00:00:00
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journal_title:Neurochemical research
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更新日期:2003-05-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章,评审
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更新日期:2008-10-01 00:00:00
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journal_title:Neurochemical research
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
abstract::Omega-conotoxin GVIA (omega-CTX), as a selective blocker for an N-type Ca2+ channel, has been conveniently used in many molecular biochemical and pharmacological experiments. There has been little elucidation of 125I-omega-CTX binding sites (mainly the 135-kDa band) in the crude membranes from chick brain, although th...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022805615926
更新日期:2003-05-01 00:00:00
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更新日期:2011-01-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
abstract::Sandhoff disease is an incurable neurodegenerative disorder caused by mutations in the lysosomal hydrolase β-hexosaminidase. Deficiency in this enzyme leads to excessive accumulation of ganglioside GM2 and its asialo derivative, GA2, in brain and visceral tissues. Small molecule inhibitors of ceramide-specific glucosy...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-0992-5
更新日期:2013-04-01 00:00:00
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journal_title:Neurochemical research
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更新日期:2008-03-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:1979-10-01 00:00:00
abstract::The volume-regulated anion channel, VRAC, plays an important role in cell volume regulation. This channel is permeable for a wide variety of anions, amino acids, and organic osmolytes, including taurine. However, nothing is known about possible water permeability of this channel. Water permeability of endothelial cell...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000010430.23137.be
更新日期:2004-01-01 00:00:00
abstract::Oxidative stress can induce neuronal apoptosis via the production of superoxide and hydroxyl radicals. This process is as a major pathogenic mechanism in neurodegenerative disorders. In this study, we aimed to clarify whether theaflavins protect PC12 cells from oxidative stress damage induced by H2O2. A cell model of ...
journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:2016-12-01 00:00:00