Abstract：
:The features of neuronal damage induced by the mitochondrial toxin NaN(3) were investigated in rat primary cortical neuron cultures. Cell viability (MTT colorimetric determination) and transmembrane mitochondrial potential (J-C1 fluorescence) were concentration-dependently reduced 24 h after NaN(3); neither nuclear fragmentation by DAPI, nor Annexin V positivity by flow cytometry were detected, ruling out the occurrence of apoptosis. The loss in cell viability (to 54 +/- 2%) observed 24 h after a 10-min treatment with 3 mM NaN(3) was prevented by the NMDA glutamate receptor antagonist MK801 (1 microM), by the antioxidants trolox (100 microM) and acetyl-L-carnitine (1 mM) and by the nitric oxide synthase inhibitor, L-NAME (100 microM), but not by the guanylylcyclase inhibitor ODQ, 10 microM. The mitochondrial dysfunction induced by NaN(3) provides a common platform for investigating the mechanisms of both ischemic and degenerative neuronal injury, useful for screening potential protective agents against neuronal death.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Selvatici R,Previati M,Marino S,Marani L,Falzarano S,Lanzoni I,Siniscalchi Adoi
10.1007/s11064-008-9852-0subject
Has Abstractpub_date
2009-05-01 00:00:00pages
909-16issue
5eissn
0364-3190issn
1573-6903journal_volume
34pub_type
杂志文章abstract：:Convulsions and brain levels of amino acids and 5-hydroxytryptamine (5-HT) in E1 mice were examined after oral administration of a 1% guanidinoethane sulfonate (GES) solution. The incidence of convulsions increased 3 days after starting GES administration, and this effect continued throughout the 6 months of drug admi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969763
更新日期：1989-01-01 00:00:00
abstract：:A prominent feature of brain pathology in Alzheimer's disease is a robust activation of the neuronal lysosomal system and major cellular pathways converging on the lysosome, namely, endocytosis and autophagy. Recent studies that identify a disturbance of the endocytic pathway as one of the earliest known manifestation...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1007675508413
更新日期：2000-10-01 00:00:00
abstract：:Suspensions of rat brain microsomes, synaptosomes, and synaptic vesicles were able to convert adenosine to inosine by means of adenosine deaminase. Isosbestic points of this transformation, at 222, 250 and 281 nm, remained unchanged with time-course. This fact suggests that adenosine deaminase (ADA, E.C. 3.5.4.4) is l...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965316
更新日期：1986-04-01 00:00:00
abstract：:A study was undertaken in rats to evaluate the effects of short-term oral ethanol administration on the levels of fatty acid ethyl esters (FAEE) in brain and peripheral organs in the presence and absence of pretreatment with L-carnitine. Administration of ethanol to rats for seven days resulted in fatty acid ethyl est...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020984114824
更新日期：1999-01-01 00:00:00
abstract：:We evaluated oxidative stress associated with a model of experimental epilepsy. Male Wistar rats were injected i.p. with 150 mg/kg convulsant 3-mercaptopropionic acid and decapitated in two stages: during seizures or in the post-seizure period. Spontaneous chemiluminescence, levels of thiobarbituric acid reactive subs...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF03177490
更新日期：1998-12-01 00:00:00
abstract：:Chemotherapy drugs such as vincristine (VCR) can cause neuropathic pain, and there is still lack of ideal strategy to treat it. The current study was designed to investigate effect of matrine (MT) on VCR-induced neuropathic pain in animal model. VCR (75 μg/kg, i.p. for 10 consecutive days) was administered to induce p...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-2040-8
更新日期：2016-11-01 00:00:00
abstract：:In this review we examine successively: 1) the major effects of thyroid hormone deficiency seen during brain development with special emphasis on the changes in neuronal morphology and migration occurring postnatally in the cerebellum. 2) The effects of this hormone on microtubule assembly during neurite outgrowth and...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00965840
更新日期：1991-09-01 00:00:00
abstract：:The preventive effect of dietary taurine supplementation on glial alterations in retina of streptozotocin-induced diabetic rats was examined in this study. Blood glucose content, content of taurine, glutamate and
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9763-0
更新日期：2009-02-01 00:00:00
abstract：:We have synthesized the tertiary amines of pyridostigmine and neostigmine, 3-pyridinol dimethylcarbamate (norpyridostigmine) and 3-dimethylaminophenol dimethylcarbamate (norneostigmine) respectively, and we have tested their abilities to cross the blood-brain barrier and inhibit mouse brain AChE activity. The in vivo ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973747
更新日期：1990-06-01 00:00:00
abstract：:Glutamine synthetase (GS) is the major glutamine-forming enzyme of vertebrates and is accepted to be a marker of astroglial cells. Maturation of astroglial cells is characterized by an increase of GS activity, and the regulation of this enzyme is the topic of many publications. Because of the fundamental role of the G...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-2754-5
更新日期：2005-05-01 00:00:00
abstract：:Clinical studies have demonstrated that decreased adiponectin is associated with the development of Type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD). We focused on determining the neuroprotective effect offered by adiponectin against streptozotocin-induced brain damage in ICV-STZ rat model. We found that a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2426-2
更新日期：2018-02-01 00:00:00
abstract：:Under some pathological conditions in brain, a large amount of superoxide anion (O(2)(-)) is produced, causing various cellular damages. Among three isozymes of superoxide dismutase (SOD), extracellular (EC)-SOD should play a role to detoxify O(2)(-) in extracellular space; however, a little is known about EC-SOD in b...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0882-2
更新日期：2013-01-01 00:00:00
abstract：:Recent studies have demonstrated a scrutinized association of diabetes mellitus with depressive symptoms and major depression. Glycogen synthase kinase-3 (GSK-3) is a protein kinase enzyme constitutively active in non-stimulated cells and in multiple signalings. Independent lines of research provide a converging evide...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1670-6
更新日期：2015-09-01 00:00:00
abstract：:The pathophysiological mechanism(s) underlying Alzheimer's disease (AD) still remain unclear, and no disease-modifying or prophylactic therapies are currently available. Unraveling the fundamental neuropathogenesis of AD is an important challenge. Several studies on AD have suggested lesions in a number of CNS areas i...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-011-0680-2
更新日期：2012-04-01 00:00:00
abstract：:The first mentioning of the word "receptor" for the structure with which a bioactive compound should react for obtaining its specific influence on a physiological system goes back to the years around 1900. The receptor concept was adapted from the lock and key theory for the enzyme substrate and blockers interactions....
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-014-1398-8
更新日期：2014-10-01 00:00:00
abstract：:The levels of selected neuroregulatory proteins that inhibit or promote apoptotic cell death were measured in the striatum of piglets subjected to precisely controlled 1 h hypoxic insult followed by 0, 2 and 4 h recovery and compared to sham operated animals. The anti-apoptotic proteins: there were increases in Surviv...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0394-x
更新日期：2011-05-01 00:00:00
abstract：:We have investigated gene expression changes produced by acute and chronic daily treatment with a prototypical antidepressant, imipramine, using DNA microarrays. The analysis of similarities in gene expression patterns among functionally related genes revealed four expression profile cluster areas that showed a highly...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000023610.89631.d6
更新日期：2004-06-01 00:00:00
abstract：:The binding of the enkephalin dimer [D-Ala2, Leu5-NH-CH2-]2 (DPE2) is characterized by its high affinity for receptors on NG108-15 hybrid cells, the affinity constant K = 4.7 X 10(9) M-1 is up to 8-fold that of monomers (0.6 to 1.0 X 10(9) M-1), and a maximal binding capacity equal to one half that of the monomers. Ki...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965208
更新日期：1986-06-01 00:00:00
abstract：:Ellagic acid (EA, 2,3,7,8-tetrahydroxy-chromeno; C14H6O8) is a polyphenol derived from fruits (pomegranates, berries) and nuts. EA exhibits antioxidant capacity and induces anti-inflammatory actions in several mammalian tissues. EA has been characterized as a possible neuroprotective agent, but the number of reports i...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-016-1853-9
更新日期：2016-06-01 00:00:00
abstract：:Due to the biological importance of sodium and its relative scarcity within many natural environments, 'salt appetite' has evolved whereby dietary salt is highly sought after and palatable when tasted. In addition to peripheral responses, salt depletion is detected within the brain via circumventricular organs and 11β...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2336-3
更新日期：2018-01-01 00:00:00
abstract：:Parkinson's disease (PD) is a neurodegenerative disease, with genetics and environment contributing to the disease onset. The limited pathological cognize of the disease restrained the approaches to improve the clinical treatment. Recently, studies showed that endoplasmic reticulum (ER) stress played an important role...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1922-0
更新日期：2016-08-01 00:00:00
abstract：:The central control of growth hormone (GH) secretion from the pituitary gland is ultimately achieved by the interaction between two hypothalamic neurohormones, somatostatin which inhibits and growth hormone-releasing hormone (GHRH) which stimulates GH release. The regulation of the somatostatin and GHRH release from t...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-005-9017-3
更新日期：2006-02-01 00:00:00
abstract：:The decline in neuronal function during aging may result from increases in extracellular glutamate (Glu), Glu-induced neurotoxicity, and altered mitochondrial metabolism. To study metabolic responses to persistently high levels of Glu at synapses during aging, we used transgenic (Tg) mice that over-express the enzyme ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1239-9
更新日期：2014-01-01 00:00:00
abstract：:Catecholamine secretion in the bovine adrenal medulla is evoked largely by nicotinic receptor activation. However, bovine adrenal medulla also contain muscarinic receptors that mediate several cell responses. To understand the physiological role of muscarinic receptors in the bovine adrenal medulla it is important to ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968406
更新日期：1992-12-01 00:00:00
abstract：:The interactions of S-nitrosoglutathione (GSNO) with the ionotropic glutamate receptors were studied on synaptic membranes isolated from the pig cerebral cortex. GSNO displaced the binding of [3H]glutamate, 3-[(R)-2-carboxypiperazin-4-yl] [3H]propyl-1-phosphonate ([3H]CPP), a competitive N-methyl-D-aspartate (NMDA) an...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007626230278
更新日期：2000-08-01 00:00:00
abstract：:Plasmalogens are ether-linked phospholipids that are abundant in nervous tissues. Their biological role is unclear, but may involve membrane structure/function and antioxidant activities. This study further investigates a recent report that chronic administration of myo-inositol in rats increased brain phosphatidyleth...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000018859.23881.f9
更新日期：2004-04-01 00:00:00
abstract：:Specific [3H]MK801 binding to rat brain NMDA receptors after the administration of the convulsant drug 3-mercaptopropionic acid (MP) and the adenosine analogue cyclopentyladenosine (CPA) was studied by means of a quantitative autoradiographic method. MP administration (150 mg/kg, i.p.) caused significant decreases in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020708603495
更新日期：1998-10-01 00:00:00
abstract：:We analyzed the impact of axonopathy-inducing agents 1,2-diacetylbenzene (1,2-DAB) and 2,5-hexanedione (2,5-HD) on membrane-bound protein disulfide isomerase (mPDI) versus soluble PDI (sPDI), or PDI-family member thioredoxin (THX), and asked whether changes in PDI/THX were associated with production of oxidative/nitro...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-9977-9
更新日期：2009-11-01 00:00:00
abstract：:Synaptosomes isolated from mouse brain were incubated with [14C]glutamate and [3H]gamma-amino-butyric acid ([3H]GABA), and then [14C]GABA (newly synthesized GABA) and [3H]GABA (newly captured GABA) in the synaptosomes were analysed. (1) the [3H]GABA was rapidly degraded in the synaptosomes, (2) when the synaptosomes w...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968675
更新日期：1990-03-01 00:00:00
abstract：:Previous studies have shown that chronic administration of SR-3 (a 1:4 mixture of alpha-linolenic and linoleic acid) affects spatial learning, thermoregulation, pain threshold and protection from seizures. The mode of action is unknown. One possible explanation is that the preparation induces changes in the fatty acid...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022430620205
更新日期：1998-05-01 00:00:00
