Abstract:
:Agonist occupancy of muscarinic cholinergic receptors in human SH-SY-5Y neuroblastoma cells elicited two kinetically distinct phases of phosphoinositide hydrolysis when monitored by either an increased mass of inositol 1,4,5-trisphosphate, or the accumulation of a total inositol phosphate fraction. Within 5s of the addition of the muscarinic agonist, oxotremorine-M, the phosphoinositide pool was hydrolyzed at a maximal rate of 9.5%/min. This initial phase of phosphoinositide hydrolysis was short-lived (t1/2 = 14s) and after 60s of agonist exposure, the rate of inositol lipid breakdown had declined to a steady state level of 3.4%/min which was then maintained for at least 5-10 min. This rapid, but partial, attenuation of muscarinic receptor stimulated phosphoinositide hydrolysis occurred prior to the agonist-induced internalization of muscarinic receptors.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Fisher SK,Slowiejko DM,McEwen ELdoi
10.1007/BF00971329subject
Has Abstractpub_date
1994-05-01 00:00:00pages
549-54issue
5eissn
0364-3190issn
1573-6903journal_volume
19pub_type
杂志文章abstract::The synthetic Liver X receptor (LXR) activator T0901317 has been reported to exert neuroprotective effect in Alzheimer's disease, but the relationship between LXR activation and beta-site amyloid precursor protein cleaving enzyme 1 (BACE-1) remains uncertain. This study investigated the effect of T0901317 on membrane ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0513-3
更新日期:2011-10-01 00:00:00
abstract::This review presents data on metabotropic uridine nucleotide receptor subtypes (P2UR) activated by UTP, sometimes also by UDP and/or ATP. Some chemical details of receptor subtypes and ligand interactions are described. Ligand-activated P2UR subtypes may couple to different second messengers, yet little is known about...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1022487128766
更新日期:1997-08-01 00:00:00
abstract::Exposure chronically to n-hexane produces peripheral-central axonopathy mediated by 2,5-hexanedione (HD). Previous studies have demonstrated decreases in neurofilament (NF) contents of peripheral and central nervous regions from rats intoxicated with HD, and recent analysis has demonstrated that axonal atrophy, instea...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-2439-5
更新日期:2005-02-01 00:00:00
abstract::The steady-state mRNA levels of NGF, BDNF and NT-3, and the mRNA levels of their receptors p75NGFR, trk, trkB, and trkC were examined in various human peripheral neuropathies, to determine the correlation with myelinated fiber pathology and T cell and macrophage invasions in the diseased nerves. Steady state levels of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022434209787
更新日期:1998-06-01 00:00:00
abstract::Hyperprolinemia type II (HPII) is an autosomal recessive disorder caused by the severe deficiency of enzyme delta1-pyrroline-5-carboxylic acid dehydrogenase leading to tissue accumulation of proline. Chronic administration of Pro led to significant reduction of cytosolic ALT activity of olfactory lobes (50.57%), cereb...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-7026-2
更新日期:2004-12-01 00:00:00
abstract::Despite immense advances in the treatment strategies, the effective treatment of patients suffering from neuropathic pain remains challenging. Saikosaponin a possesses anti-inflammatory activity. However, the role of saikosaponin a in neuropathic pain is still unclear. Therefore, the objective of this study was to inv...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1407-y
更新日期:2014-11-01 00:00:00
abstract::The concentration of vitamin E (alpha-tocopherol) was measured in samples of cortex from patients with Alzheimer's disease (AD), fetuses with Down's syndrome (DS), and also in a group of centenarians. The mean tocopherol concentrations in the two patient groups did not differ significantly from appropriate controls. W...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965511
更新日期:1989-12-01 00:00:00
abstract::The enzymes degrading leucine enkephalin in human plasma and the inhibitors active on these enzymes were studied by kinetic and chromatographic techniques. Data obtained evidence the existence of complex kinetics of leu-enkephalin hydrolysis and of formation of its hydrolysis by-products. These appear to originate fro...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00995551
更新日期:1995-09-01 00:00:00
abstract::Acidosis (pH 6.0) led to significant decrease in high-affinity choline uptake by rat brain synaptosomes. The effects persisted following pH readjustment (7.4) of the incubation medium, consisting of decrease in both Km and Vmax of the affinity system. pH readjustment coincided with synaptosomal leakage of lactate dehy...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969699
更新日期:1995-07-01 00:00:00
abstract::The effects of carbachol (CCh) on the frequency (f) of the miniature endplate potentials were tested at temperatures between 5 and 30 degrees C. Higher CCh concentrations, 1 x 10(-5) and 5 x 10(-6) M, reduced the f to 60% and the temperature dependence was negligible. However, an inverse temperature dependence was fou...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1012372115058
更新日期:2001-09-01 00:00:00
abstract::The effect of maternal hypoxia on the modification of the fetal brain cell membrane N-methyl-D-aspartate (NMDA) receptor and its modulatory sites was investigated. Experiments were conducted in pregnant guinea pigs of 60 days of gestation. Guinea pig fetuses were exposed to maternal hypoxia (FiO2 = 7%) for 60 minutes....
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968402
更新日期:1992-12-01 00:00:00
abstract::Axonal regeneration across the site of spinal cord lesion is often aborted in adult mammalian species. The use of DNA vaccine to nullify the inhibitory molecules has been shown to be effective in promoting axonal regeneration in injured spinal cord. The possible molecular mechanisms, however, remain to be elucidated. ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-9951-6
更新日期:2009-09-01 00:00:00
abstract::Plasmalogens are ether-linked phospholipids that are abundant in nervous tissues. Their biological role is unclear, but may involve membrane structure/function and antioxidant activities. This study further investigates a recent report that chronic administration of myo-inositol in rats increased brain phosphatidyleth...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000018859.23881.f9
更新日期:2004-04-01 00:00:00
abstract::Memory impairment is a major problem afflicting mankind. The association between memory functions and neurotransmitter functions is of great interest for understanding brain function. Serotonergic pathways play an important role in the modulation of memory functions but the importance of its receptor types and subtype...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0839-5
更新日期:2012-10-01 00:00:00
abstract::Germline mutations in genes encoding succinate dehydrogenase subunits are associated with the development of familial pheochromocytomas and paragangliomas [hereditary paraganglioma/pheochromocytoma syndrome (HPPS)]. In particular, a mutation in succinate dehydrogenase subunit B (SDHB) is highly associated with abdomin...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1738-3
更新日期:2016-04-01 00:00:00
abstract::Attempts were made to solubilize acetylcholinesterase (AChE) from microsomal membranes isolated from rabbit white muscle. The preparative procedure included a step in which the microsomes were incubated in a solution containing high salt concentration (0.6 M KCl). About 15% of the total enzyme activity could be solubi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971007
更新日期:1987-07-01 00:00:00
abstract::The effects of pre-treatment with ENA-713, an acetylcholinesterase (AChE) inhibitor, on changes in pre- and postsynaptic cholinergic indices in gerbil brain following transient ischemia were studied at 4 and 14 days after recirculation. In the ischemic group, hippocampal acetylcholine (ACh) level was significantly red...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966804
更新日期:1994-02-01 00:00:00
abstract::Several previous studies utilizing selective pharmacological antagonists have demonstrated that type 5 metabotropic glutamate receptors (mGluR5) are potential therapeutic targets for the treatment of numerous disorders of the central nervous system, but the role of mGluR5 activation in traumatic brain injury (TBI) is ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0691-z
更新日期:2012-05-01 00:00:00
abstract::The vanilloid receptor 1(VR1) is a nonselective cation channel that is activated by pungent vanilloid compound, extracellular protons, or noxious heat. mRNA of VR1 and vanilloid receptor 1-like receptor (VRL1) were expressed in PC12 cells, and only VRI mRNA was detected in glioma and A10 cell lines. VRI protein was de...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000018839.59457.5c
更新日期:2004-04-01 00:00:00
abstract::Antiretroviral protease inhibitors are a class of important drugs that are used for the treatment of human immunodeficiency virus infections. Among those compounds, ritonavir is applied frequently in combination with other antiretroviral protease inhibitors, as it has been reported to boost their therapeutic efficienc...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-0971-x
更新日期:2013-04-01 00:00:00
abstract::The potent parkinsonian neurotoxin 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) is known to cause dopaminergic neurodegeneration in nigrostriatal system. In the present study we investigated the nuclear morphology of cells in the substantia nigra pars compacta (SNpc) region of rats following unilateral intra...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9299-8
更新日期:2007-07-01 00:00:00
abstract::Amyloidogenic proteins (Abeta peptide) in Alzheimer's disease (AD) and alpha-synuclein (alpha-Syn) in Parkinson's disease (PD) are typically soluble monomeric precursors, which undergo remarkable conformational changes and culminate in the form of aggregates in diseased condition. Overlap of clinical and neuropatholog...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9140-9
更新日期:2006-09-01 00:00:00
abstract::Metabolic turnover of axonally transported glycoproteins has been examined in membranous and soluble subfractions of goldfish optic tectum following intraocular injection of [3H]fucose. Radioactivity in total transported glycoproteins reached a maximum in the tectum after 24-30 hr, then declined with a half-life of ap...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964224
更新日期:1980-04-01 00:00:00
abstract::Regional and developmental changes in metabolite concentrations were measured by 1H NMR spectroscopy and HPLC of perchloric acid extracts from rat brain and eye. The highest concentrations of N-acetylaspartate were found in grey matter as opposed to white matter with concentration increasing with age from neonate to a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02532417
更新日期:1996-09-01 00:00:00
abstract::The current study demonstrates that in hippocampal neurons mitochondrial Ca(2+) processing supports Ca(2+) influx via ionotropic glutamate (Glu) receptors. We define mitochondrial Ca(2+) processing as Ca(2+) uptake via mitochondrial Ca(2+) uniporter (MCU) combined with subsequent Ca(2+) release via mitochondrial Na(+)...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1819-3
更新日期:2016-06-01 00:00:00
abstract::The preventive effect of dietary taurine supplementation on glial alterations in retina of streptozotocin-induced diabetic rats was examined in this study. Blood glucose content, content of taurine, glutamate and
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9763-0
更新日期:2009-02-01 00:00:00
abstract::Disruptive effects of calcium upon neurofilaments and glial filaments were studied in white matter of rat optic nerve and spinal cord and in rat peripheral nerve. Filament ultrastructure and tissue protein composition were compared following a calcium influx into excised tissues. A calcium influx was induced by freeze...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964040
更新日期:1981-03-01 00:00:00
abstract::We review here the possible mechanisms of neuronal degeneration caused by L-cysteine, an odd excitotoxin. L-Cysteine lacks the omega carboxyl group required for excitotoxic actions via excitatory amino acid receptors, yet it evokes N-methyl-D-aspartate (NMDA) -like excitotoxic neuronal death and potentiates the Ca2+ i...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1007616817499
更新日期:2000-10-01 00:00:00
abstract::Hypothalamic and plasma dopamine-beta-hydroxylase [DBH, EC 1.14.2.1] activites were measured by a coupled radioenzymatic method. Animals representing five experimental groups [intact controls, adrenalectomized, pinealectomized, adrenalectomized pinealectomized, doubly sham-operated] were killed and sampled at 8 times ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965575
更新日期:1978-06-01 00:00:00
abstract::Experimental evidence has suggested that the high endogenous levels of taurine found in the rat heart and retina are maintained to a large extent by transport processes out of the blood, rather than by endogenous biosynthesis. When these high levels are depleted, dysfunction ensues. In vitro studies have shown that be...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970775
更新日期:1988-10-01 00:00:00